Rh2BG017300

FAD-NAD(P)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007219
Physical Location & Seq
Reverse (-)
1266033 .. 1268858
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG017300.1

Sequence Viewer

Length: 1284 bp
ATGCGGACACCTTCACCGCTTACAGTAGTCTCACCTTCACCTTCACCTTCATCTCCCTCCTCTTCTTCTCCTTTTCTCTTCTCTCATTCTCAGAGTCCTTCTCGTCTTCGGTACGCCGTCCTCGGCGCTGGCTTCGCCGGCCTCTCCGTCGCTTACCACTTGCTGAAGCAGTGTCCCAAGGACTCGAATCTCCGCATCGACGTTTTCGATGAAGTCGGAATCGGCGGCGGCGCTTCCGGCGTCTCCGGCGGCCTTCTTCACCCTTACTCTCCCAAAGCCAAGCTCCTATGGCGCGCCGCCGAGTGCTGGGATGAGGCTTTGAGGCTGTTGAACGTCGCCGAAGCTGAACTGCCTCCCAACTCCGATGATGCTCCCATTGTTAGAAGAAGAGGCATCTTGAGACCAGCTTTGAGCATGAAGAACATGATGGTGCTGAAAGATAATGCCCAGAATGGCCTTGCCAGTTGCAGAATAGAAACCATTGACGAGGATAGTGCCCAAAATCTTGTACCCGGCATCTGTTCACCTTTCAATACATCCTTTTATATGCCTGAAGCTGTTAATGTCCATCCTCAACGCTATCTCGAGGCACTCTTCTTAGCGTGTCAAACTTTGGTGAAAGAACTTTGTGCTTCTGGATCGGGGGTAAAAGAATTGCATTTGCACAAGGACTCCATTCACAAACTGCTAGACTTGGAAGGGGAATACCAGGCAGTAATTGTATGTCTAGGAGCCAAAGCAGATCTGCTTCCTGAGCTCTCTGGGAGGCTTCCATTGAGGACATGCAGAGGTGTCGTTGCACATCTGCAGCTTCCTGATGACATGGGAGGGGAGTATCCAGGCTTTGGCCCCTCAATATTGTCAGATGCTTGGCTTGCTGTCCAAGGACCTCGTAGTTTGTATATGGGCTCAACATGGGAATGGAAATCAAGAAATTCATCATCAAACGTATCTGCAGATGAAGCTACTGAAGCCCTTAAAGAGCTTCTACCAAAGGCATGCGGCATTTATCCAGTTGTAAAAGATTGGACCTTTATTGGAGCCAGGGCTGGCCTTAGGGCAATGCCACCACTCACACCTCATGGGTCACTTCCTCTTTTGGGATGTGTCAATAACATTATAGGTCAAAATCAAACTTGCAAATACTGGTTATTTGGAGGGCTTGGTTCAAGGGGATTGTTGTACCATGGTTGGCTTGGGAAGTTGATGGCACAAGCTGTGCTTTCGTGTAATGAAGAATTAATTCCATATGAATTAACCTCTTGGAAAAGAACAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

427

Amino Acids

46.33

Weight (kDa)

7.54

Isoelectric Point (pI)

59.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 38 - 405 1.6e-39 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 845
AccII CGCG 1 cut(s) 294
AciI CCGC 8 cut(s) 4, 17, 193, 225, 228, 249, 297, 1002
AclWI GGATC 1 cut(s) 646
AcsI RAATTY 1 cut(s) 934
AcuI CTGAAG 3 cut(s) 185, 573, 990
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 3 cut(s) 113, 510, 1184
AfiI CCNNNNNNNGG 3 cut(s) 306, 845, 1100
AgsI TTSAA 3 cut(s) 331, 532, 1170
AjnI CCWGG 3 cut(s) 708, 838, 1043
AluBI AGCT 9 cut(s) 283, 344, 407, 557, 757, 811, 965, 985, 1217
AluI AGCT 9 cut(s) 283, 344, 407, 557, 757, 811, 965, 985, 1217
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 3 cut(s) 34, 247, 394
AlwI GGATC 1 cut(s) 646
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 5 cut(s) 139, 250, 454, 847, 1051
ApeKI GCWGC 1 cut(s) 808
ApoI RAATTY 1 cut(s) 934
AscI GGCGCGCC 1 cut(s) 292
AseI ATTAAT 1 cut(s) 1241
Asp700I GAANNNNTTC 1 cut(s) 1242
AspLEI GCGC 4 cut(s) 128, 233, 294, 296
AspS9I GGNCC 3 cut(s) 848, 887, 1029
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 7 cut(s) 6, 24, 30, 36, 251, 516, 628
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 2 cut(s) 887, 1029
AxyI CCTNAGG 1 cut(s) 1055
BaeGI GKGCMC 1 cut(s) 499
BanII GRGCYC 2 cut(s) 759, 911
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 1 cut(s) 820
BccI CCATC 3 cut(s) 421, 576, 1201
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 4 cut(s) 476, 510, 775, 809
BciT130I CCWGG 3 cut(s) 710, 840, 1045
BciVI GTATCC 1 cut(s) 846
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 3 cut(s) 34, 247, 394
BfaI CTAG 2 cut(s) 689, 728
BfmI CTRYAG 2 cut(s) 806, 954
BfoI RGCGCY 2 cut(s) 129, 234
BfuI GTATCC 1 cut(s) 846
BglII AGATCT 1 cut(s) 742
BisI GCNGC 6 cut(s) 226, 229, 250, 297, 809, 1003
BlsI GCNGC 6 cut(s) 227, 230, 251, 298, 810, 1004
Bme1390I CCNGG 4 cut(s) 513, 710, 840, 1045
Bme18I GGWCC 2 cut(s) 887, 1029
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 3 cut(s) 848, 887, 1029
BmiI GGNNCC 3 cut(s) 733, 850, 1042
BmrFI CCNGG 4 cut(s) 513, 710, 840, 1045
BmsI GCATC 5 cut(s) 204, 358, 402, 525, 856
BpiI GAAGAC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 85, 117
Bpu10I CCTNAGC 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 418
BpuMI CCSGG 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 394
BsaJI CCNNGG 5 cut(s) 121, 177, 883, 1044, 1186
BsaXI ACNNNNNCTCC 2 cut(s) 656, 686
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 845, 1100
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 3 cut(s) 462, 1013, 1151
Bse21I CCTNAGG 1 cut(s) 1055
Bse3DI GCAATG 1 cut(s) 1068
BseBI CCWGG 3 cut(s) 710, 840, 1045
BseDI CCNNGG 5 cut(s) 121, 177, 883, 1044, 1186
BseGI GGATG 4 cut(s) 316, 536, 568, 1109
BseLI CCNNNNNNNGG 3 cut(s) 306, 845, 1100
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 2 cut(s) 104, 744
BseNI ACTGG 3 cut(s) 462, 1013, 1151
BsePI GCGCGC 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 1 cut(s) 820
BseYI CCCAGC 1 cut(s) 306
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 5 cut(s) 141, 252, 456, 849, 1053
BsiHKAI GWGCWC 1 cut(s) 759
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 4 cut(s) 138, 237, 246, 513
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 3 cut(s) 306, 845, 1100
BsmAI GTCTC 3 cut(s) 34, 247, 394
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 5 cut(s) 141, 252, 456, 849, 1053
Bso31I GGTCTC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 3 cut(s) 499, 759, 911
Bsp143I GATC 2 cut(s) 638, 742
Bsp19I CCATGG 1 cut(s) 1186
BspACI CCGC 8 cut(s) 4, 17, 193, 225, 228, 249, 297, 1002
BspANI GGCC 5 cut(s) 141, 252, 456, 849, 1053
BspCNI CTCAG 2 cut(s) 103, 745
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 733, 850, 1042
BspMAI CTGCAG 2 cut(s) 810, 958
BspPI GGATC 1 cut(s) 646
BspTNI GGTCTC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 1068
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 3 cut(s) 462, 1013, 1151
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 5 cut(s) 121, 177, 883, 1044, 1186
BssHII GCGCGC 1 cut(s) 292
BssMI GATC 2 cut(s) 638, 742
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 3 cut(s) 177, 883, 1186
Bst2UI CCWGG 3 cut(s) 710, 840, 1045
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 4 cut(s) 67, 83, 382, 599
BstACI GRCGYC 1 cut(s) 240
BstC8I GCNNGC 6 cut(s) 130, 139, 294, 876, 1000, 1051
BstDEI CTNAG 4 cut(s) 90, 598, 753, 1055
BstDSI CCRYGG 1 cut(s) 1186
BstF5I GGATG 4 cut(s) 316, 536, 568, 1109
BstFNI CGCG 1 cut(s) 294
BstH2I RGCGCY 2 cut(s) 129, 234
BstHHI GCGC 4 cut(s) 128, 233, 294, 296
BstKTI GATC 2 cut(s) 641, 745
BstMAI GTCTC 3 cut(s) 34, 247, 394
BstMBI GATC 2 cut(s) 638, 742
BstMWI GCNNNNNNNGC 9 cut(s) 134, 138, 237, 246, 289, 754, 875, 962, 971
BstNI CCWGG 3 cut(s) 710, 840, 1045
BstNSI RCATGY 2 cut(s) 786, 1002
BstSCI CCNGG 4 cut(s) 511, 708, 838, 1043
BstSFI CTRYAG 2 cut(s) 806, 954
BstSLI GKGCMC 1 cut(s) 499
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 820
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
Bsu36I CCTNAGG 1 cut(s) 1055
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 5 cut(s) 141, 252, 456, 849, 1053
BtgI CCRYGG 1 cut(s) 1186
BtsCI GGATG 4 cut(s) 316, 536, 568, 1109
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 176
Cac8I GCNNGC 6 cut(s) 130, 139, 294, 876, 1000, 1051
CfoI GCGC 4 cut(s) 128, 233, 294, 296
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 3 cut(s) 848, 887, 1029
CseI GACGC 1 cut(s) 229
Csp6I GTAC 3 cut(s) 112, 509, 1183
CviAII CATG 8 cut(s) 415, 424, 783, 823, 915, 999, 1082, 1187
CviQI GTAC 3 cut(s) 112, 509, 1183
DdeI CTNAG 4 cut(s) 90, 598, 753, 1055
DpnI GATC 2 cut(s) 640, 744
DpnII GATC 2 cut(s) 638, 742
Eam1104I CTCTTC 4 cut(s) 67, 83, 382, 599
EarI CTCTTC 4 cut(s) 67, 83, 382, 599
Ecl136II GAGCTC 1 cut(s) 757
Eco130I CCWWGG 3 cut(s) 177, 883, 1186
Eco24I GRGCYC 2 cut(s) 759, 911
Eco31I GGTCTC 1 cut(s) 394
Eco47I GGWCC 2 cut(s) 887, 1029
Eco53kI GAGCTC 1 cut(s) 757
Eco57I CTGAAG 3 cut(s) 185, 573, 990
Eco81I CCTNAGG 1 cut(s) 1055
Eco88I CYCGRG 1 cut(s) 584
EcoICRI GAGCTC 1 cut(s) 757
EcoO109I RGGNCCY 1 cut(s) 887
EcoRII CCWGG 3 cut(s) 708, 838, 1043
EcoT14I CCWWGG 3 cut(s) 177, 883, 1186
EcoT38I GRGCYC 2 cut(s) 759, 911
ErhI CCWWGG 3 cut(s) 177, 883, 1186
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 8 cut(s) 418, 427, 786, 826, 918, 1002, 1085, 1190
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FaqI GGGAC 1 cut(s) 159
FatI CATG 8 cut(s) 414, 423, 782, 822, 914, 998, 1081, 1186
FauNDI CATATG 1 cut(s) 1249
Fnu4HI GCNGC 6 cut(s) 226, 229, 250, 297, 809, 1003
FokI GGATG 4 cut(s) 323, 523, 555, 1116
FriOI GRGCYC 2 cut(s) 759, 911
Fsp4HI GCNGC 6 cut(s) 226, 229, 250, 297, 809, 1003
FspBI CTAG 2 cut(s) 689, 728
GlaI GCGC 4 cut(s) 127, 232, 293, 295
GluI GCNGC 6 cut(s) 226, 229, 250, 297, 809, 1003
GsaI CCCAGC 1 cut(s) 310
HaeII RGCGCY 2 cut(s) 129, 234
HaeIII GGCC 5 cut(s) 141, 252, 456, 849, 1053
HapII CCGG 4 cut(s) 138, 237, 246, 513
HgaI GACGC 1 cut(s) 229
HhaI GCGC 4 cut(s) 128, 233, 294, 296
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 8 cut(s) 418, 427, 786, 826, 918, 1002, 1085, 1190
Hin6I GCGC 4 cut(s) 126, 231, 292, 294
HinP1I GCGC 4 cut(s) 126, 231, 292, 294
HinfI GANTC 5 cut(s) 94, 182, 187, 219, 671
HpaII CCGG 4 cut(s) 138, 237, 246, 513
HphI GGTGA 7 cut(s) 6, 24, 30, 36, 251, 516, 628
Hpy166II GTNNAC 1 cut(s) 524
Hpy188I TCNGA 4 cut(s) 93, 218, 364, 865
Hpy188III TCNNGA 6 cut(s) 397, 584, 636, 752, 815, 930
Hpy8I GTNNAC 1 cut(s) 524
Hpy99I CGWCG 3 cut(s) 152, 203, 338
HpyAV CCTTC 7 cut(s) 21, 45, 51, 57, 108, 263, 692
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 3 cut(s) 201, 333, 948
HpyCH4V TGCA 8 cut(s) 468, 658, 664, 786, 800, 808, 956, 1140
HpyF10VI GCNNNNNNNGC 9 cut(s) 134, 138, 237, 246, 289, 754, 875, 962, 971
HpyF3I CTNAG 4 cut(s) 90, 598, 753, 1055
HpySE526I ACGT 3 cut(s) 201, 333, 948
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 8 cut(s) 418, 427, 786, 826, 918, 1002, 1085, 1190
HspAI GCGC 4 cut(s) 126, 231, 292, 294
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 638, 742
LmnI GCTCC 4 cut(s) 288, 376, 731, 1040
Lsp1109I GCAGC 1 cut(s) 820
LweI GCATC 5 cut(s) 204, 358, 402, 525, 856
MaeI CTAG 2 cut(s) 689, 728
MaeII ACGT 3 cut(s) 201, 333, 948
MaeIII GTNAC 1 cut(s) 1086
MalI GATC 2 cut(s) 640, 744
MboI GATC 2 cut(s) 638, 742
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 3 cut(s) 499, 759, 911
MluCI AATT 6 cut(s) 653, 717, 934, 1238, 1242, 1253
MlyI GAGTC 3 cut(s) 103, 176, 665
MmeI TCCRAC 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 1242
MseI TTAA 4 cut(s) 561, 978, 1241, 1256
MslI CAYNNNNRTG 2 cut(s) 428, 919
MspI CCGG 4 cut(s) 138, 237, 246, 513
MspR9I CCNGG 4 cut(s) 513, 710, 840, 1045
MvaI CCWGG 3 cut(s) 710, 840, 1045
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 9 cut(s) 134, 138, 237, 246, 289, 754, 875, 962, 971
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 513
NcoI CCATGG 1 cut(s) 1186
NdeI CATATG 1 cut(s) 1249
NdeII GATC 2 cut(s) 638, 742
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 8 cut(s) 418, 427, 786, 826, 918, 1002, 1085, 1190
NlaIV GGNNCC 3 cut(s) 733, 850, 1042
NmeAIII GCCGAG 2 cut(s) 102, 325
NmuCI GTSAC 1 cut(s) 1086
NspI RCATGY 2 cut(s) 786, 1002
PaeI GCATGC 1 cut(s) 1002
PaeR7I CTCGAG 1 cut(s) 584
PalAI GGCGCGCC 1 cut(s) 292
PauI GCGCGC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 1242
PfeI GAWTC 2 cut(s) 187, 219
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 6 cut(s) 227, 230, 251, 298, 810, 1004
PleI GAGTC 3 cut(s) 102, 176, 665
PpsI GAGTC 3 cut(s) 102, 176, 665
PpuMI RGGWCCY 1 cut(s) 887
PshBI ATTAAT 1 cut(s) 1241
Psp124BI GAGCTC 1 cut(s) 759
Psp5II RGGWCCY 1 cut(s) 887
Psp6I CCWGG 3 cut(s) 708, 838, 1043
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 3 cut(s) 708, 838, 1043
PspN4I GGNNCC 3 cut(s) 733, 850, 1042
PspPI GGNCC 3 cut(s) 848, 887, 1029
PspPPI RGGWCCY 1 cut(s) 887
PstI CTGCAG 2 cut(s) 810, 958
PsuI RGATCY 1 cut(s) 742
PteI GCGCGC 1 cut(s) 292
RsaI GTAC 3 cut(s) 113, 510, 1184
RsaNI GTAC 3 cut(s) 112, 509, 1183
RseI CAYNNNNRTG 2 cut(s) 428, 919
SacI GAGCTC 1 cut(s) 759
SaqAI TTAA 4 cut(s) 561, 978, 1241, 1256
SatI GCNGC 6 cut(s) 226, 229, 250, 297, 809, 1003
Sau3AI GATC 2 cut(s) 638, 742
Sau96I GGNCC 3 cut(s) 848, 887, 1029
SchI GAGTC 3 cut(s) 103, 176, 665
ScrFI CCNGG 4 cut(s) 513, 710, 840, 1045
SduI GDGCHC 3 cut(s) 499, 759, 911
SfaNI GCATC 5 cut(s) 204, 358, 402, 525, 856
SfcI CTRYAG 2 cut(s) 806, 954
Sfr274I CTCGAG 1 cut(s) 584
SgsI GGCGCGCC 1 cut(s) 292
SinI GGWCC 2 cut(s) 887, 1029
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 2 cut(s) 428, 919
SmlI CTYRAG 2 cut(s) 397, 584
SmoI CTYRAG 2 cut(s) 397, 584
SphI GCATGC 1 cut(s) 1002
Sse9I AATT 6 cut(s) 653, 717, 934, 1238, 1242, 1253
SsiI CCGC 8 cut(s) 4, 17, 193, 225, 228, 249, 297, 1002
SspI AATATT 1 cut(s) 858
SspMI CTAG 2 cut(s) 689, 728
SstI GAGCTC 1 cut(s) 759
StyD4I CCNGG 4 cut(s) 511, 708, 838, 1043
StyI CCWWGG 3 cut(s) 177, 883, 1186
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 3 cut(s) 204, 336, 951
TaqI TCGA 4 cut(s) 185, 198, 207, 585
TasI AATT 6 cut(s) 653, 717, 934, 1238, 1242, 1253
TauI GCSGC 5 cut(s) 228, 231, 252, 299, 1005
TfiI GAWTC 2 cut(s) 187, 219
Tru1I TTAA 4 cut(s) 561, 978, 1241, 1256
Tru9I TTAA 4 cut(s) 561, 978, 1241, 1256
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 1 cut(s) 1086
TseI GCWGC 1 cut(s) 808
Tsp45I GTSAC 1 cut(s) 1086
TspDTI ATGAA 7 cut(s) 39, 225, 431, 927, 975, 1248, 1266
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 1 cut(s) 176
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 2 cut(s) 887, 1029
VspI ATTAAT 1 cut(s) 1241
XapI RAATTY 1 cut(s) 934
XceI RCATGY 2 cut(s) 786, 1002
XcmI CCANNNNNNNNNTGG 1 cut(s) 1193
XhoI CTCGAG 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 1242
XspI CTAG 2 cut(s) 689, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.