Rroxscaffold_2G00154610

FAD-NAD(P)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007219
Physical Location & Seq
Forward (+)
91178148 .. 91181225
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154610.1

Sequence Viewer

Length: 417 bp
ATGAAGAACATGATGGTGCTGAAAGATAATGCCCAGAATGGCCTTGCCAGTTGTAGAATAGAAACCATTGACGAGGATGGTGCCCAAAATCTTGTACCCGGCATCTGTTCACCTCTCAATACGTCTTTTTATATGTCTGAAGCTGTTAATGTCCATCCTCAACGCTATCTCGAGGCACTCTTCTTAGCATGTCAAACTTTGGTGAAAGAACTTTGTGCTTCTGGATTGGGGGTAAAAGAATTGCATTTGCACAAGGACTCCATTCACAAACTGCTAGACTTGGAAGGGGAATACCAGGCAGTAATTGTATGTCTAGGAGCCAAAGCAGATCTGCTTCCTGAGCTCTCTGGGAGGCTTCCATTGGGGACATGCAGAGGTGTCGTTGCACATCTGCAGCTTCCTGATAATATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

138

Amino Acids

14.99

Weight (kDa)

5.43

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 13 - 135 2.4e-08 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 1 cut(s) 96
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 3 cut(s) 143, 343, 397
AluI AGCT 3 cut(s) 143, 343, 397
Alw21I GWGCWC 1 cut(s) 345
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 394
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 102, 214
AvaI CYCGRG 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 406
BccI CCATC 3 cut(s) 7, 71, 162
BcgI CGANNNNNNTGC 4 cut(s) 62, 96, 361, 395
BciT130I CCWGG 1 cut(s) 296
BcnI CCSGG 1 cut(s) 99
BfaI CTAG 2 cut(s) 275, 314
BfmI CTRYAG 1 cut(s) 392
BglII AGATCT 1 cut(s) 328
BisI GCNGC 1 cut(s) 395
BlsI GCNGC 1 cut(s) 396
Bme1390I CCNGG 2 cut(s) 99, 296
BmeT110I CYCGRG 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 82, 319
BmrFI CCNGG 2 cut(s) 99, 296
BmsI GCATC 1 cut(s) 111
Bpu10I CCTNAGC 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bse1I ACTGG 1 cut(s) 48
BseBI CCWGG 1 cut(s) 296
BseGI GGATG 2 cut(s) 82, 154
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 1 cut(s) 406
BshFI GGCC 1 cut(s) 42
BshNI GGYRCC 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 1 cut(s) 42
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 85, 345
Bsp143I GATC 1 cut(s) 328
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 82, 319
BspMAI CTGCAG 1 cut(s) 396
BspT107I GGYRCC 1 cut(s) 80
BsrI ACTGG 1 cut(s) 48
BssMI GATC 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 184, 339
BstF5I GGATG 2 cut(s) 82, 154
BstKTI GATC 1 cut(s) 331
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 2 cut(s) 192, 372
BstSCI CCNGG 2 cut(s) 97, 294
BstSFI CTRYAG 1 cut(s) 392
BstSLI GKGCMC 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 1 cut(s) 42
BtsCI GGATG 2 cut(s) 82, 154
Csp6I GTAC 1 cut(s) 95
CviAII CATG 3 cut(s) 10, 189, 369
CviJI RGCY 6 cut(s) 42, 143, 320, 343, 355, 397
CviKI_1 RGCY 6 cut(s) 42, 143, 320, 343, 355, 397
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 2 cut(s) 184, 339
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 345
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 345
FaeI CATG 3 cut(s) 13, 192, 372
FaiI YATR 7 cut(s) 11, 132, 134, 190, 310, 370, 410
FaqI GGGAC 1 cut(s) 379
FatI CATG 3 cut(s) 9, 188, 368
Fnu4HI GCNGC 1 cut(s) 395
FokI GGATG 2 cut(s) 89, 141
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 395
FspBI CTAG 2 cut(s) 275, 314
GluI GCNGC 1 cut(s) 395
HaeIII GGCC 1 cut(s) 42
HapII CCGG 1 cut(s) 99
Hin1II CATG 3 cut(s) 13, 192, 372
HinfI GANTC 1 cut(s) 257
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 102, 214
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 4 cut(s) 170, 222, 338, 401
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 5 cut(s) 244, 250, 372, 386, 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 2 cut(s) 184, 339
HpySE526I ACGT 1 cut(s) 122
Hsp92II CATG 3 cut(s) 13, 192, 372
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 8 cut(s) 47, 61, 112, 207, 281, 308, 333, 351
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 1 cut(s) 111
MaeI CTAG 2 cut(s) 275, 314
MaeII ACGT 1 cut(s) 122
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 2 cut(s) 16, 172
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 85, 345
MluCI AATT 2 cut(s) 239, 303
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 6 cut(s) 67, 123, 166, 168, 345, 368
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 99, 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 340
NciI CCSGG 1 cut(s) 99
NdeII GATC 1 cut(s) 328
NlaIII CATG 3 cut(s) 13, 192, 372
NlaIV GGNNCC 2 cut(s) 82, 319
NspI RCATGY 2 cut(s) 192, 372
PaeR7I CTCGAG 1 cut(s) 170
PkrI GCNGC 1 cut(s) 396
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 82, 319
PstI CTGCAG 1 cut(s) 396
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 1 cut(s) 395
Sau3AI GATC 1 cut(s) 328
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 2 cut(s) 99, 296
SduI GDGCHC 2 cut(s) 85, 345
SetI ASST 6 cut(s) 115, 125, 145, 345, 379, 399
SfaNI GCATC 1 cut(s) 111
SfcI CTRYAG 1 cut(s) 392
Sfr274I CTCGAG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 2 cut(s) 239, 303
SspMI CTAG 2 cut(s) 275, 314
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 97, 294
TaiI ACGT 1 cut(s) 125
TaqI TCGA 1 cut(s) 171
TasI AATT 2 cut(s) 239, 303
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseI GCWGC 1 cut(s) 394
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 2 cut(s) 192, 372
XhoI CTCGAG 1 cut(s) 170
XspI CTAG 2 cut(s) 275, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.