AT2G23420

nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005674
Physical Location & Seq
Forward (+)
9971603 .. 9975207
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23420.1

Sequence Viewer

Length: 1674 bp
ATGGAGCCGAAAGAGAACGGCTCAGAATTGGGTCAGAAGATCATTGACGGACCAACGAATCCAATGGTCACACCTTTACTCAATGATCTTTATCAATTCACCATGGCTTATGCTTATTGGAAAGCTGGCAAACACAACGAACGATCCGTTTTCGATCTGTATTTTCGTAAGAACCCATTTGGTGGTGAGTACACTGTGTTTGCTGGATTAGAAGAGTGTGTTAAGTTCTTAGCCAATTTCAAATTGACTGATGAAGAAATCGATTTCGTTCAAGAGTGTTTGCCTGGATCTGAGGAAGCTTTTTGTGATTATCTTAGAGGGCTTGATTGTTCTGATGTTGAAGTTTATGCAATTCCGGAAGGATCAGTTGTTTTTCCTAAAGTACCTCTCATGAGAGTTGAAGGACCTGTTGGTGTTGTTCAATTGTTGGAAACTCCATTCCTCAATCTTGTCAATTTTGCATCTTTGGTAGCTACTAACGCAGCTAGGCATCGCTTTGTTGCCGGAAAATCTAAGAGTCTACTCGAGTTTGGTGCTCGAAGGGCTCAGGGTCCGGATGGTGCAATAAGCGCATCAAAATATTGCTACCTTGGAGGTTTTGATGCAACAAGTAATGTAGCAGCTGGAAAACTTTTTGGGATTCCTCTTCGTGGAACACACTCTCATGCTTATGTTAGCTCATTCATGAGTACTGATGAGATTGTTGACAAAGTACTTCGTAGTGCTGATGGGAAAACCACGTGCGAGGATTTTGTTAGTCATGTTCAGACATGGTTAAAAAAGATTCAGTATTCACCATCTCTAAGTGGCATTTTCTCTGAGACAAATCAAAGCGAGCTAGCAGCTTTCACCTCATATGCACTGGCATTCCCCAAAACTTTTCTTGCCCTCGTAGATACATACGATGTGATGAAGAGTGGAATCCCTAACTTCTGTGCAGTTGCTTTAGCACTCAATGACTTTGGATATAAAGCATTAGGTATTAGACTGGATTCAGGTGATTTAGCTTATCTATCTAGAGAGGCCAGAAATTTCTTCTGCACGGTAGAGAGAGAACTAAAAGTGCCTGGTTTTGGGAAGATGGTCGTCACTGCTAGTAATGATCTAAATGAAGAGACGATTGACGCTTTAAATAAACAGGGACATGAGGTGGATGCTTTTGGCATCGGGACCTACTTGGTCACTTGCTATTCACAAGCGGCCTTAGGTTGCGTTTTCAAACTTGTGGAGATAAACAATCAGCCTCGGATTAAACTTTCTGAAGATGTTACAAAGGTATCAATACCGTGTAAAAAGCGAAGTTACAGATTATACGGCAAAGAAGGTTACCCTCTGGTAGATATAATGACTGGAGAGAACGAACCACCTCCAAAGGTTGGTGAGCGTTTACTTTGTCGTCACCCATTCAACGAATCCAAAAGAGCATATGTAGTGCCACAACGTGTCGAAGAGCTCCTCAAATGTTATTGGCGTGGAAGTGCAGATGAAGCAAGAGAAGTATTACCGCCTTTGAAAGAGATAAGAGACCGTTGCATCAAACAGCTCGAAAACATGCGACCTGATCATATGAGGAGATTAAACCCAACTCCTTATAAGGTTAGTGTAAGCGCAAAGCTGTACGATTTCATCCACTTCTTATGGCTAAACGAAGCACCTGTTGGTGAATTGCAGTGA

Protein Analysis

557

Amino Acids

62.27

Weight (kDa)

5.86

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 26 - 152 1.6e-39 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 110 - 155 1.3e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase PF04095 174 - 425 6.5e-06 Nicotinate phosphoribosyltransferase (NAPRTase) family
NAPRTase_C PF17956 431 - 542 1.7e-31 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1593
AccB7I CCANNNNNTGG 2 cut(s) 182, 1376
AccI GTMKAC 1 cut(s) 520
AccIII TCCGGA 2 cut(s) 355, 553
AciI CCGC 2 cut(s) 1199, 1505
AclWI GGATC 3 cut(s) 138, 295, 370
AcsI RAATTY 1 cut(s) 1030
AcuI CTGAAG 1 cut(s) 1281
AcvI CACGTG 1 cut(s) 741
AdeI CACNNNGTG 1 cut(s) 1442
AfaI GTAC 5 cut(s) 191, 384, 691, 714, 1619
AfiI CCNNNNNNNGG 4 cut(s) 182, 650, 1073, 1376
AflIII ACRYGT 1 cut(s) 1441
AgsI TTSAA 8 cut(s) 241, 272, 341, 401, 422, 1219, 1408, 1513
AjnI CCWGG 2 cut(s) 283, 1066
AjuI GAANNNNNNNTTGG 4 cut(s) 393, 425, 1641, 1673
Alw21I GWGCWC 2 cut(s) 538, 1455
Alw26I GTCTC 3 cut(s) 815, 1109, 1518
AlwI GGATC 3 cut(s) 138, 295, 370
Ama87I CYCGRG 1 cut(s) 524
Aor13HI TCCGGA 2 cut(s) 355, 553
AoxI GGCC 2 cut(s) 1023, 1200
ApeKI GCWGC 3 cut(s) 482, 620, 842
ApoI RAATTY 1 cut(s) 1030
AspLEI GCGC 2 cut(s) 572, 1610
AspS9I GGNCC 4 cut(s) 50, 404, 551, 1170
AsuHPI GGTGA 8 cut(s) 91, 197, 786, 841, 1010, 1391, 1391, 1673
AsuNHI GCTAGC 1 cut(s) 838
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 4 cut(s) 50, 404, 551, 1170
AxyI CCTNAGG 1 cut(s) 1204
BaeI ACNNNNGTAYC 2 cut(s) 1260, 1293
BanII GRGCYC 2 cut(s) 547, 1455
BbrPI CACGTG 1 cut(s) 741
Bbv12I GWGCWC 2 cut(s) 538, 1455
BbvI GCAGC 3 cut(s) 494, 632, 854
BccI CCATC 4 cut(s) 551, 722, 805, 1075
BceAI ACGGC 2 cut(s) 34, 1330
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 2 cut(s) 285, 1068
BclI TGATCA 1 cut(s) 1561
BcoDI GTCTC 3 cut(s) 815, 1109, 1518
BfaI CTAG 4 cut(s) 486, 839, 1017, 1095
BisI GCNGC 4 cut(s) 483, 621, 843, 1200
BlsI GCNGC 4 cut(s) 484, 622, 844, 1201
BmcAI AGTACT 2 cut(s) 691, 714
Bme1390I CCNGG 2 cut(s) 285, 1068
Bme18I GGWCC 4 cut(s) 50, 404, 551, 1170
BmeT110I CYCGRG 1 cut(s) 524
BmgT120I GGNCC 4 cut(s) 50, 404, 551, 1170
BmiI GGNNCC 3 cut(s) 6, 552, 1171
BmrFI CCNGG 2 cut(s) 285, 1068
BmsI GCATC 7 cut(s) 470, 499, 581, 592, 1144, 1173, 1542
BmtI GCTAGC 1 cut(s) 842
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 1371
Bpu10I CCTNAGC 1 cut(s) 546
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 1518
BsaJI CCNNGG 3 cut(s) 102, 589, 1244
BsaWI WCCGGW 2 cut(s) 355, 553
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 650, 1073, 1376
Bse1I ACTGG 3 cut(s) 867, 993, 1354
Bse21I CCTNAGG 1 cut(s) 1204
Bse8I GATNNNNATC 1 cut(s) 90
BseAI TCCGGA 2 cut(s) 355, 553
BseBI CCWGG 2 cut(s) 285, 1068
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 3 cut(s) 102, 589, 1244
BseGI GGATG 3 cut(s) 562, 1159, 1626
BseJI GATNNNNATC 1 cut(s) 90
BseLI CCNNNNNNNGG 4 cut(s) 182, 650, 1073, 1376
BseMII CTCAG 4 cut(s) 36, 282, 560, 810
BseNI ACTGG 3 cut(s) 867, 993, 1354
BseRI GAGGAG 2 cut(s) 1445, 1585
BseXI GCAGC 3 cut(s) 494, 632, 854
BsgI GTGCAG 3 cut(s) 957, 1024, 1500
BshFI GGCC 2 cut(s) 1025, 1202
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 538, 1455
BsiHKCI CYCGRG 1 cut(s) 524
BsiSI CCGG 3 cut(s) 356, 504, 554
BslFI GGGAC 2 cut(s) 1155, 1183
BslI CCNNNNNNNGG 4 cut(s) 182, 650, 1073, 1376
BsmAI GTCTC 3 cut(s) 815, 1109, 1518
BsmBI CGTCTC 1 cut(s) 1109
BsmFI GGGAC 2 cut(s) 1155, 1183
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 2 cut(s) 1025, 1202
Bso31I GGTCTC 1 cut(s) 1518
BsoBI CYCGRG 1 cut(s) 524
Bsp1286I GDGCHC 3 cut(s) 538, 547, 1455
Bsp13I TCCGGA 2 cut(s) 355, 553
Bsp143I GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 2 cut(s) 1199, 1505
BspANI GGCC 2 cut(s) 1025, 1202
BspCNI CTCAG 4 cut(s) 35, 283, 559, 811
BspDI ATCGAT 1 cut(s) 261
BspEI TCCGGA 2 cut(s) 355, 553
BspHI TCATGA 2 cut(s) 390, 684
BspLI GGNNCC 3 cut(s) 6, 552, 1171
BspOI GCTAGC 1 cut(s) 842
BspPI GGATC 3 cut(s) 138, 295, 370
BspQI GCTCTTC 1 cut(s) 1443
BspTNI GGTCTC 1 cut(s) 1518
BsrI ACTGG 3 cut(s) 867, 993, 1354
BssECI CCNNGG 3 cut(s) 102, 589, 1244
BssMI GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
BssT1I CCWWGG 2 cut(s) 102, 589
Bst2UI CCWGG 2 cut(s) 285, 1068
Bst4CI ACNGT 4 cut(s) 196, 1045, 1287, 1529
Bst6I CTCTTC 5 cut(s) 207, 651, 908, 1107, 1443
BstBAI YACGTR 1 cut(s) 741
BstC8I GCNNGC 3 cut(s) 127, 836, 840
BstDEI CTNAG 9 cut(s) 22, 229, 291, 314, 513, 546, 803, 819, 1204
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 1325
BstF5I GGATG 3 cut(s) 562, 1159, 1626
BstHHI GCGC 2 cut(s) 572, 1610
BstKTI GATC 8 cut(s) 42, 88, 146, 157, 290, 365, 1105, 1564
BstMAI GTCTC 3 cut(s) 815, 1109, 1518
BstMBI GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
BstMWI GCNNNNNNNGC 4 cut(s) 479, 542, 569, 1487
BstNI CCWGG 2 cut(s) 285, 1068
BstNSI RCATGY 1 cut(s) 1555
BstPI GGTNACC 1 cut(s) 1325
BstSCI CCNGG 2 cut(s) 283, 1066
BstV1I GCAGC 3 cut(s) 494, 632, 854
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
Bsu15I ATCGAT 1 cut(s) 261
Bsu36I CCTNAGG 1 cut(s) 1204
BsuRI GGCC 2 cut(s) 1025, 1202
BsuTUI ATCGAT 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 476
BtsCI GGATG 3 cut(s) 562, 1159, 1626
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 3 cut(s) 192, 860, 1089
Cac8I GCNNGC 3 cut(s) 127, 836, 840
CciI TCATGA 2 cut(s) 390, 684
CfoI GCGC 2 cut(s) 572, 1610
Cfr13I GGNCC 4 cut(s) 50, 404, 551, 1170
ClaI ATCGAT 1 cut(s) 261
CseI GACGC 1 cut(s) 1133
Csp6I GTAC 5 cut(s) 190, 383, 690, 713, 1618
CviAII CATG 8 cut(s) 103, 391, 665, 685, 761, 771, 1145, 1552
CviQI GTAC 5 cut(s) 190, 383, 690, 713, 1618
DdeI CTNAG 9 cut(s) 22, 229, 291, 314, 513, 546, 803, 819, 1204
DpnI GATC 8 cut(s) 41, 87, 145, 156, 289, 364, 1104, 1563
DpnII GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
DraI TTTAAA 1 cut(s) 1131
DraIII CACNNNGTG 1 cut(s) 1442
Eam1104I CTCTTC 5 cut(s) 207, 651, 908, 1107, 1443
EarI CTCTTC 5 cut(s) 207, 651, 908, 1107, 1443
Ecl136II GAGCTC 1 cut(s) 1453
Eco130I CCWWGG 2 cut(s) 102, 589
Eco24I GRGCYC 2 cut(s) 547, 1455
Eco31I GGTCTC 1 cut(s) 1518
Eco47I GGWCC 4 cut(s) 50, 404, 551, 1170
Eco53kI GAGCTC 1 cut(s) 1453
Eco57I CTGAAG 1 cut(s) 1281
Eco72I CACGTG 1 cut(s) 741
Eco81I CCTNAGG 1 cut(s) 1204
Eco88I CYCGRG 1 cut(s) 524
Eco91I GGTNACC 1 cut(s) 1325
EcoICRI GAGCTC 1 cut(s) 1453
EcoO109I RGGNCCY 2 cut(s) 404, 1170
EcoO65I GGTNACC 1 cut(s) 1325
EcoRII CCWGG 2 cut(s) 283, 1066
EcoT14I CCWWGG 2 cut(s) 102, 589
EcoT38I GRGCYC 2 cut(s) 547, 1455
ErhI CCWWGG 2 cut(s) 102, 589
Esp3I CGTCTC 1 cut(s) 1109
FaeI CATG 8 cut(s) 106, 394, 668, 688, 764, 774, 1148, 1555
FaqI GGGAC 2 cut(s) 1155, 1183
FatI CATG 8 cut(s) 102, 390, 664, 684, 760, 770, 1144, 1551
FauNDI CATATG 3 cut(s) 856, 1426, 1566
FbaI TGATCA 1 cut(s) 1561
FblI GTMKAC 1 cut(s) 520
Fnu4HI GCNGC 4 cut(s) 483, 621, 843, 1200
FokI GGATG 3 cut(s) 569, 1166, 1613
FriOI GRGCYC 2 cut(s) 547, 1455
Fsp4HI GCNGC 4 cut(s) 483, 621, 843, 1200
FspBI CTAG 4 cut(s) 486, 839, 1017, 1095
GlaI GCGC 2 cut(s) 571, 1609
GluI GCNGC 4 cut(s) 483, 621, 843, 1200
GsuI CTGGAG 1 cut(s) 1371
HaeIII GGCC 2 cut(s) 1025, 1202
HapII CCGG 3 cut(s) 356, 504, 554
HgaI GACGC 1 cut(s) 1133
HhaI GCGC 2 cut(s) 572, 1610
Hin1II CATG 8 cut(s) 106, 394, 668, 688, 764, 774, 1148, 1555
Hin6I GCGC 2 cut(s) 570, 1608
HinP1I GCGC 2 cut(s) 570, 1608
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 7 cut(s) 58, 517, 640, 784, 921, 992, 1412
HpaII CCGG 3 cut(s) 356, 504, 554
HphI GGTGA 8 cut(s) 91, 197, 786, 841, 1010, 1391, 1391, 1673
Hpy166II GTNNAC 4 cut(s) 192, 521, 706, 1388
Hpy188I TCNGA 8 cut(s) 25, 36, 292, 334, 768, 820, 1248, 1261
Hpy188III TCNNGA 7 cut(s) 272, 356, 391, 554, 685, 1017, 1168
Hpy8I GTNNAC 4 cut(s) 192, 521, 706, 1388
HpyAV CCTTC 4 cut(s) 353, 395, 534, 1316
HpyCH4III ACNGT 4 cut(s) 196, 1045, 1287, 1529
HpyCH4IV ACGT 2 cut(s) 740, 1441
HpyF10VI GCNNNNNNNGC 4 cut(s) 479, 542, 569, 1487
HpyF3I CTNAG 9 cut(s) 22, 229, 291, 314, 513, 546, 803, 819, 1204
HpySE526I ACGT 2 cut(s) 740, 1441
Hsp92II CATG 8 cut(s) 106, 394, 668, 688, 764, 774, 1148, 1555
HspAI GCGC 2 cut(s) 570, 1608
Kpn2I TCCGGA 2 cut(s) 355, 553
Ksp22I TGATCA 1 cut(s) 1561
Kzo9I GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
LguI GCTCTTC 1 cut(s) 1443
LmnI GCTCC 2 cut(s) 4, 1458
Lsp1109I GCAGC 3 cut(s) 494, 632, 854
LweI GCATC 7 cut(s) 470, 499, 581, 592, 1144, 1173, 1542
MaeI CTAG 4 cut(s) 486, 839, 1017, 1095
MaeII ACGT 2 cut(s) 740, 1441
MaeIII GTNAC 7 cut(s) 67, 1087, 1180, 1267, 1301, 1325, 1397
MalI GATC 8 cut(s) 41, 87, 145, 156, 289, 364, 1104, 1563
MboI GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
MfeI CAATTG 1 cut(s) 422
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 3 cut(s) 538, 547, 1455
MluCI AATT 9 cut(s) 26, 95, 235, 242, 351, 422, 454, 1030, 1664
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 408
MroI TCCGGA 2 cut(s) 355, 553
MseI TTAA 5 cut(s) 222, 776, 1130, 1251, 1577
MslI CAYNNNNRTG 2 cut(s) 663, 669
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 3 cut(s) 356, 504, 554
MspR9I CCNGG 2 cut(s) 285, 1068
MunI CAATTG 1 cut(s) 422
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 2 cut(s) 285, 1068
MwoI GCNNNNNNNGC 4 cut(s) 479, 542, 569, 1487
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 3 cut(s) 856, 1426, 1566
NdeII GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
NheI GCTAGC 1 cut(s) 838
NlaIII CATG 8 cut(s) 106, 394, 668, 688, 764, 774, 1148, 1555
NlaIV GGNNCC 3 cut(s) 6, 552, 1171
NmuCI GTSAC 4 cut(s) 67, 1087, 1180, 1397
NspI RCATGY 1 cut(s) 1555
PaeR7I CTCGAG 1 cut(s) 524
PagI TCATGA 2 cut(s) 390, 684
PciSI GCTCTTC 1 cut(s) 1443
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 866
PfeI GAWTC 6 cut(s) 58, 640, 784, 921, 992, 1412
PflMI CCANNNNNTGG 2 cut(s) 182, 1376
PkrI GCNGC 4 cut(s) 484, 622, 844, 1201
PleI GAGTC 1 cut(s) 525
PmaCI CACGTG 1 cut(s) 741
PmlI CACGTG 1 cut(s) 741
PpsI GAGTC 1 cut(s) 525
Ppu21I YACGTR 1 cut(s) 741
PpuMI RGGWCCY 2 cut(s) 404, 1170
PsiI TTATAA 1 cut(s) 1593
Psp124BI GAGCTC 1 cut(s) 1455
Psp5II RGGWCCY 2 cut(s) 404, 1170
Psp6I CCWGG 2 cut(s) 283, 1066
PspCI CACGTG 1 cut(s) 741
PspEI GGTNACC 1 cut(s) 1325
PspGI CCWGG 2 cut(s) 283, 1066
PspN4I GGNNCC 3 cut(s) 6, 552, 1171
PspPI GGNCC 4 cut(s) 50, 404, 551, 1170
PspPPI RGGWCCY 2 cut(s) 404, 1170
PspXI VCTCGAGB 1 cut(s) 524
PsuI RGATCY 1 cut(s) 287
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 5 cut(s) 191, 384, 691, 714, 1619
RsaNI GTAC 5 cut(s) 190, 383, 690, 713, 1618
RseI CAYNNNNRTG 2 cut(s) 663, 669
SacI GAGCTC 1 cut(s) 1455
SapI GCTCTTC 1 cut(s) 1443
SaqAI TTAA 5 cut(s) 222, 776, 1130, 1251, 1577
SatI GCNGC 4 cut(s) 483, 621, 843, 1200
Sau3AI GATC 8 cut(s) 39, 85, 143, 154, 287, 362, 1102, 1561
Sau96I GGNCC 4 cut(s) 50, 404, 551, 1170
ScaI AGTACT 2 cut(s) 691, 714
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 2 cut(s) 285, 1068
SduI GDGCHC 3 cut(s) 538, 547, 1455
SfaNI GCATC 7 cut(s) 470, 499, 581, 592, 1144, 1173, 1542
Sfr274I CTCGAG 1 cut(s) 524
SinI GGWCC 4 cut(s) 50, 404, 551, 1170
SlaI CTCGAG 1 cut(s) 524
SmiMI CAYNNNNRTG 2 cut(s) 663, 669
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 9 cut(s) 26, 95, 235, 242, 351, 422, 454, 1030, 1664
SsiI CCGC 2 cut(s) 1199, 1505
SspI AATATT 1 cut(s) 581
SspMI CTAG 4 cut(s) 486, 839, 1017, 1095
SstI GAGCTC 1 cut(s) 1455
StyD4I CCNGG 2 cut(s) 283, 1066
StyI CCWWGG 2 cut(s) 102, 589
TaaI ACNGT 4 cut(s) 196, 1045, 1287, 1529
TaiI ACGT 2 cut(s) 743, 1444
TaqI TCGA 6 cut(s) 153, 261, 525, 538, 1446, 1545
TasI AATT 9 cut(s) 26, 95, 235, 242, 351, 422, 454, 1030, 1664
TatI WGTACW 3 cut(s) 189, 689, 712
TauI GCSGC 1 cut(s) 1202
TfiI GAWTC 6 cut(s) 58, 640, 784, 921, 992, 1412
Tru1I TTAA 5 cut(s) 222, 776, 1130, 1251, 1577
Tru9I TTAA 5 cut(s) 222, 776, 1130, 1251, 1577
TscAI CASTG 3 cut(s) 199, 867, 1096
TseFI GTSAC 4 cut(s) 67, 1087, 1180, 1397
TseI GCWGC 3 cut(s) 482, 620, 842
Tsp45I GTSAC 4 cut(s) 67, 1087, 1180, 1397
TspDTI ATGAA 6 cut(s) 267, 673, 926, 1125, 1500, 1615
TspGWI ACGGA 2 cut(s) 63, 136
TspRI CASTG 3 cut(s) 199, 867, 1096
Van91I CCANNNNNTGG 2 cut(s) 182, 1376
VpaK11BI GGWCC 4 cut(s) 50, 404, 551, 1170
XapI RAATTY 1 cut(s) 1030
XbaI TCTAGA 1 cut(s) 1016
XceI RCATGY 1 cut(s) 1555
XhoI CTCGAG 1 cut(s) 524
XmiI GTMKAC 1 cut(s) 520
XspI CTAG 4 cut(s) 486, 839, 1017, 1095
ZrmI AGTACT 2 cut(s) 691, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.