AT4G36940

nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005674
Physical Location & Seq
Forward (+)
17416855 .. 17420252
3398 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G36940.1

Sequence Viewer

Length: 1680 bp
ATGGAGAAGAAAGAAAATGGTCTCGATGGAAAGCAATCGGGTCGGGTCATTAACGGACCCACTAACCCGATGGTCACACCTCTGCTCAACGATCTTTACCAATTCACCATGGCTTATGCTTATTGGAAAGCTGGCAAACAATCTGAGCGATCTGTGTTTGATCTGTATTTTCGTAAGAATCCTTTTGGTGGAGAATACACTATCTTTGCTGGTTTAGAAGAATGCATCAAATTTCTCGCTAATTTCAATTTGACTGATGAAGAGATCGATTTCGTTCGTGATTCGTTACCTGGATGTGAGGAAGCTTTCTGTGATTATCTTCGAGGGCTTGATTGTTCTGACATTGAAGTGTATGCCATTTCGGAAGGATCAGTTGTTTTTCCTAAAGTTCCTTTACTCAGAATCGAAGGTCCTGTTGCTGTGGTGCAATTGTTGGAAACTCCATTCCTCAATCTCATCAATTACGCATCTTTGGTTGCTACAAATGCAGCAAGACATCGGTTTGTTGCAGGAAAATCTAAGCTTCTGCTTGAGTTTGGTGCTAGAAGAGCTCAGGGACCCGATGGTGCAATAAGCGCATCAAAGTATTGCTACCTTGGAGGTTTTGATGCAACAAGTAATGTTGCAGCGGGAAAACTGTTTGGGATACCCCTCCGTGGTACTCATTCCCATGCTTTTGTTAGCTCATTCATGAGCCTTGATGAAATTGTTGACAAAGTGCTTCGAAGTTCTGATGGGAAAAGCACTTGTAAGGATTTTATATGTTTGGTCCAAACTTGCCTAACAAAGATTCAGAATTCATCTTCATTACAAGGAATTTTTTCCGAGACAAATCAAAGCGAGCTTGCAGCGTTCATTTCATATGCACTGGCATTCCCAAACTCCTTTCTCGCTCTTGTAGACACTTATGATGTGATGAAGAGTGGTATTCCAAACTTCTGTGCTGTTGCTCTAGCACTTAATGAATTGGGATACAAAGCAGTAGGCATTAGACTGGATTCAGGTGACTTAGCCTATCTTTCTACTGAGGTCAGGAAATTCTTTTGTGCCATAGAGAGAGACCTCAAAGTTCCTGATTTCGGGAAGATGATCGTCACTGCTAGTAACGATCTAAACGAAGAGACAGTCGATGCTCTAAATAAACAGGGTCATGAAGTAGATGCATTTGGAATTGGAACCAACTTAGTGACTTGCTATGCGCAAGCTGCGTTAGGTTGTGTTTTCAAACTTGTGGAAATAAACAATCAGCCTCGGATCAAACTTTCTGAAGATGTTACTAAGGTATCGATTCCATGTAAAAAGCGTACTTACAGATTGTTCGGAAAAGAGGGTTACCCTCTTGTTGATATAATGACTGGAGAGAACGAACCACCTCCAAAGGTCGGTGAAAGGTTACTTTGCCGTCATCCATTCAATGAATCAAAAAGGGCTTATGTGGTTCCACAACGCGTTGAAGAGCTTCTGAAATGTTATTGGCGTGGCAATGCAGATGAAGCTAGGGAAGAGCTAGAGCCATTGAAAGAGCTAAGAAATCGTTGCATCAAACAGCTCGAAAATATGCGACCCGATCATATGAGAAGATTAAACCCTACTCCTTATAAGGTTAGTGTCAGCGCCAAGTTGTATGACTTCATCCACTTCCTCTGGCTCAACGAAGCTCCTGTCGGTGAACTGCATTGA

Protein Analysis

559

Amino Acids

62.42

Weight (kDa)

5.91

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 28 - 154 8.8e-40 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 114 - 157 2.7e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 433 - 544 5e-31 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1599
Acc16I TGCGCA 1 cut(s) 1200
AccI GTMKAC 1 cut(s) 900
AccII CGCG 1 cut(s) 1449
AciI CCGC 1 cut(s) 629
AclWI GGATC 2 cut(s) 376, 1262
AcsI RAATTY 4 cut(s) 230, 796, 816, 1037
AcuI CTGAAG 1 cut(s) 1287
AfaI GTAC 2 cut(s) 661, 1306
AfiI CCNNNNNNNGG 4 cut(s) 188, 656, 1079, 1382
AflIII ACRYGT 1 cut(s) 1447
AgsI TTSAA 6 cut(s) 247, 347, 1225, 1414, 1454, 1519
AjnI CCWGG 1 cut(s) 289
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 26, 821, 1053, 1115
AlwI GGATC 2 cut(s) 376, 1262
ApeKI GCWGC 4 cut(s) 488, 626, 848, 1205
ApoI RAATTY 4 cut(s) 230, 796, 816, 1037
ArsI GACNNNNNNTTYG 4 cut(s) 1110, 1142, 1647, 1679
Asp700I GAANNNNTTC 2 cut(s) 820, 1458
AspLEI GCGC 3 cut(s) 578, 1201, 1616
AspS9I GGNCC 4 cut(s) 56, 410, 557, 769
AsuHPI GGTGA 4 cut(s) 97, 1016, 1397, 1679
AsuII TTCGAA 1 cut(s) 724
AvaII GGWCC 4 cut(s) 56, 410, 557, 769
BaeI ACNNNNGTAYC 2 cut(s) 1266, 1299
BanII GRGCYC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 4 cut(s) 500, 638, 860, 1192
BccI CCATC 4 cut(s) 20, 64, 557, 728
BceAI ACGGC 1 cut(s) 1386
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 1 cut(s) 291
BciVI GTATCC 2 cut(s) 639, 965
BcoDI GTCTC 4 cut(s) 26, 821, 1053, 1115
BfaI CTAG 5 cut(s) 543, 953, 1101, 1497, 1508
BfoI RGCGCY 1 cut(s) 1617
BfuI GTATCC 2 cut(s) 639, 965
BisI GCNGC 4 cut(s) 489, 627, 849, 1206
BlsI GCNGC 4 cut(s) 490, 628, 850, 1207
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 4 cut(s) 56, 410, 557, 769
BmgT120I GGNCC 4 cut(s) 56, 410, 557, 769
BmiI GGNNCC 5 cut(s) 58, 558, 559, 1177, 1440
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 7 cut(s) 234, 476, 587, 598, 1120, 1150, 1548
BpmI CTGGAG 1 cut(s) 1377
Bpu10I CCTNAGC 1 cut(s) 552
Bpu14I TTCGAA 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 551
Bsa29I ATCGAT 2 cut(s) 267, 1286
BsaI GGTCTC 2 cut(s) 26, 1053
BsaJI CCNNGG 4 cut(s) 108, 595, 655, 1250
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 656, 1079, 1382
Bse1I ACTGG 3 cut(s) 873, 999, 1360
Bse3DI GCAATG 1 cut(s) 1489
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 2 cut(s) 267, 1286
BseDI CCNNGG 4 cut(s) 108, 595, 655, 1250
BseGI GGATG 3 cut(s) 299, 1405, 1632
BseLI CCNNNNNNNGG 4 cut(s) 188, 656, 1079, 1382
BseMI GCAATG 1 cut(s) 1489
BseMII CTCAG 4 cut(s) 135, 412, 566, 1017
BseNI ACTGG 3 cut(s) 873, 999, 1360
BseXI GCAGC 4 cut(s) 500, 638, 860, 1192
Bsh1236I CGCG 1 cut(s) 1449
BshVI ATCGAT 2 cut(s) 267, 1286
BsiHKAI GWGCWC 1 cut(s) 553
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 4 cut(s) 188, 656, 1079, 1382
BsmAI GTCTC 4 cut(s) 26, 821, 1053, 1115
BsmFI GGGAC 1 cut(s) 570
BsmI GAATGC 2 cut(s) 227, 872
Bso31I GGTCTC 2 cut(s) 26, 1053
Bsp119I TTCGAA 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 553
Bsp143I GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 1 cut(s) 629
BspCNI CTCAG 4 cut(s) 136, 411, 565, 1018
BspDI ATCGAT 2 cut(s) 267, 1286
BspFNI CGCG 1 cut(s) 1449
BspHI TCATGA 2 cut(s) 690, 1150
BspLI GGNNCC 5 cut(s) 58, 558, 559, 1177, 1440
BspPI GGATC 2 cut(s) 376, 1262
BspQI GCTCTTC 3 cut(s) 541, 1449, 1497
BspT104I TTCGAA 1 cut(s) 724
BspTNI GGTCTC 2 cut(s) 26, 1053
BsrDI GCAATG 1 cut(s) 1489
BsrI ACTGG 3 cut(s) 873, 999, 1360
BssECI CCNNGG 4 cut(s) 108, 595, 655, 1250
BssMI GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
BssT1I CCWWGG 2 cut(s) 108, 595
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 639, 1126
Bst6I CTCTTC 6 cut(s) 255, 541, 914, 1113, 1449, 1497
BstBI TTCGAA 1 cut(s) 724
BstC8I GCNNGC 4 cut(s) 133, 842, 846, 1203
BstDEI CTNAG 9 cut(s) 144, 398, 519, 552, 1009, 1026, 1183, 1278, 1526
BstDSI CCRYGG 2 cut(s) 108, 655
BstEII GGTNACC 1 cut(s) 1331
BstF5I GGATG 3 cut(s) 299, 1405, 1632
BstFNI CGCG 1 cut(s) 1449
BstH2I RGCGCY 1 cut(s) 1617
BstHHI GCGC 3 cut(s) 578, 1201, 1616
BstKTI GATC 9 cut(s) 94, 152, 163, 267, 371, 1092, 1111, 1257, 1570
BstMAI GTCTC 4 cut(s) 26, 821, 1053, 1115
BstMBI GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
BstMWI GCNNNNNNNGC 5 cut(s) 485, 548, 575, 1205, 1493
BstNI CCWGG 1 cut(s) 291
BstPI GGTNACC 1 cut(s) 1331
BstSCI CCNGG 1 cut(s) 289
BstUI CGCG 1 cut(s) 1449
BstV1I GCAGC 4 cut(s) 500, 638, 860, 1192
Bsu15I ATCGAT 2 cut(s) 267, 1286
BsuI GTATCC 2 cut(s) 639, 965
BsuTUI ATCGAT 2 cut(s) 267, 1286
BtgI CCRYGG 2 cut(s) 108, 655
BtsCI GGATG 3 cut(s) 299, 1405, 1632
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 2 cut(s) 866, 1095
Cac8I GCNNGC 4 cut(s) 133, 842, 846, 1203
CciI TCATGA 2 cut(s) 690, 1150
CfoI GCGC 3 cut(s) 578, 1201, 1616
Cfr13I GGNCC 4 cut(s) 56, 410, 557, 769
ClaI ATCGAT 2 cut(s) 267, 1286
Csp6I GTAC 2 cut(s) 660, 1305
CviAII CATG 5 cut(s) 109, 671, 691, 1151, 1293
CviQI GTAC 2 cut(s) 660, 1305
DdeI CTNAG 9 cut(s) 144, 398, 519, 552, 1009, 1026, 1183, 1278, 1526
DpnI GATC 9 cut(s) 93, 151, 162, 266, 370, 1091, 1110, 1256, 1569
DpnII GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
Eam1104I CTCTTC 6 cut(s) 255, 541, 914, 1113, 1449, 1497
EarI CTCTTC 6 cut(s) 255, 541, 914, 1113, 1449, 1497
Ecl136II GAGCTC 1 cut(s) 551
Eco130I CCWWGG 2 cut(s) 108, 595
Eco24I GRGCYC 1 cut(s) 553
Eco31I GGTCTC 2 cut(s) 26, 1053
Eco47I GGWCC 4 cut(s) 56, 410, 557, 769
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 1287
Eco91I GGTNACC 1 cut(s) 1331
EcoICRI GAGCTC 1 cut(s) 551
EcoO109I RGGNCCY 2 cut(s) 410, 557
EcoO65I GGTNACC 1 cut(s) 1331
EcoRI GAATTC 1 cut(s) 796
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 108, 595
EcoT22I ATGCAT 2 cut(s) 227, 1165
EcoT38I GRGCYC 1 cut(s) 553
ErhI CCWWGG 2 cut(s) 108, 595
FaeI CATG 5 cut(s) 112, 674, 694, 1154, 1296
FaqI GGGAC 1 cut(s) 570
FatI CATG 5 cut(s) 108, 670, 690, 1150, 1292
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 2 cut(s) 862, 1572
FblI GTMKAC 1 cut(s) 900
Fnu4HI GCNGC 4 cut(s) 489, 627, 849, 1206
FokI GGATG 3 cut(s) 306, 1392, 1619
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 4 cut(s) 489, 627, 849, 1206
FspBI CTAG 5 cut(s) 543, 953, 1101, 1497, 1508
FspI TGCGCA 1 cut(s) 1200
GlaI GCGC 3 cut(s) 577, 1200, 1615
GluI GCNGC 4 cut(s) 489, 627, 849, 1206
GsuI CTGGAG 1 cut(s) 1377
HaeII RGCGCY 1 cut(s) 1617
HhaI GCGC 3 cut(s) 578, 1201, 1616
Hin1II CATG 5 cut(s) 112, 674, 694, 1154, 1296
Hin6I GCGC 3 cut(s) 576, 1199, 1614
HinP1I GCGC 3 cut(s) 576, 1199, 1614
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 2 cut(s) 303, 521
HinfI GANTC 7 cut(s) 178, 281, 402, 790, 998, 1288, 1418
HphI GGTGA 4 cut(s) 97, 1016, 1397, 1679
Hpy166II GTNNAC 3 cut(s) 712, 901, 1670
Hpy188III TCNNGA 7 cut(s) 23, 278, 691, 1033, 1073, 1081, 1151
Hpy8I GTNNAC 3 cut(s) 712, 901, 1670
HpyAV CCTTC 2 cut(s) 359, 401
HpyCH4III ACNGT 2 cut(s) 639, 1126
HpyF10VI GCNNNNNNNGC 5 cut(s) 485, 548, 575, 1205, 1493
HpyF3I CTNAG 9 cut(s) 144, 398, 519, 552, 1009, 1026, 1183, 1278, 1526
Hsp92II CATG 5 cut(s) 112, 674, 694, 1154, 1296
HspAI GCGC 3 cut(s) 576, 1199, 1614
KflI GGGWCCC 1 cut(s) 557
Kzo9I GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
LguI GCTCTTC 3 cut(s) 541, 1449, 1497
LmnI GCTCC 1 cut(s) 1663
Lsp1109I GCAGC 4 cut(s) 500, 638, 860, 1192
LweI GCATC 7 cut(s) 234, 476, 587, 598, 1120, 1150, 1548
MaeI CTAG 5 cut(s) 543, 953, 1101, 1497, 1508
MaeIII GTNAC 9 cut(s) 73, 285, 1004, 1093, 1103, 1186, 1273, 1331, 1392
MalI GATC 9 cut(s) 93, 151, 162, 266, 370, 1091, 1110, 1256, 1569
MboI GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
MfeI CAATTG 1 cut(s) 428
MhlI GDGCHC 1 cut(s) 553
MluI ACGCGT 1 cut(s) 1447
MmeI TCCRAC 1 cut(s) 414
Mph1103I ATGCAT 2 cut(s) 227, 1165
MroXI GAANNNNTTC 2 cut(s) 820, 1458
MseI TTAA 3 cut(s) 51, 960, 1583
MslI CAYNNNNRTG 2 cut(s) 347, 669
MspA1I CMGCKG 1 cut(s) 629
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 2 cut(s) 227, 872
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 1449
MwoI GCNNNNNNNGC 5 cut(s) 485, 548, 575, 1205, 1493
NcoI CCATGG 1 cut(s) 108
NdeI CATATG 2 cut(s) 862, 1572
NdeII GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
NlaIII CATG 5 cut(s) 112, 674, 694, 1154, 1296
NlaIV GGNNCC 5 cut(s) 58, 558, 559, 1177, 1440
NmuCI GTSAC 4 cut(s) 73, 1004, 1093, 1186
NsbI TGCGCA 1 cut(s) 1200
NsiI ATGCAT 2 cut(s) 227, 1165
NspV TTCGAA 1 cut(s) 724
PagI TCATGA 2 cut(s) 690, 1150
PciSI GCTCTTC 3 cut(s) 541, 1449, 1497
PcsI WCGNNNNNNNCGW 1 cut(s) 1113
PctI GAATGC 2 cut(s) 227, 872
PdmI GAANNNNTTC 2 cut(s) 820, 1458
PfeI GAWTC 7 cut(s) 178, 281, 402, 790, 998, 1288, 1418
PkrI GCNGC 4 cut(s) 490, 628, 850, 1207
PpuMI RGGWCCY 2 cut(s) 410, 557
PsiI TTATAA 1 cut(s) 1599
Psp124BI GAGCTC 1 cut(s) 553
Psp5II RGGWCCY 2 cut(s) 410, 557
Psp6I CCWGG 1 cut(s) 289
PspEI GGTNACC 1 cut(s) 1331
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 5 cut(s) 58, 558, 559, 1177, 1440
PspPI GGNCC 4 cut(s) 56, 410, 557, 769
PspPPI RGGWCCY 2 cut(s) 410, 557
RsaI GTAC 2 cut(s) 661, 1306
RsaNI GTAC 2 cut(s) 660, 1305
RseI CAYNNNNRTG 2 cut(s) 347, 669
SacI GAGCTC 1 cut(s) 553
SapI GCTCTTC 3 cut(s) 541, 1449, 1497
SaqAI TTAA 3 cut(s) 51, 960, 1583
SatI GCNGC 4 cut(s) 489, 627, 849, 1206
Sau3AI GATC 9 cut(s) 91, 149, 160, 264, 368, 1089, 1108, 1254, 1567
Sau96I GGNCC 4 cut(s) 56, 410, 557, 769
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 7 cut(s) 234, 476, 587, 598, 1120, 1150, 1548
SfuI TTCGAA 1 cut(s) 724
SinI GGWCC 4 cut(s) 56, 410, 557, 769
SmiMI CAYNNNNRTG 2 cut(s) 347, 669
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SsiI CCGC 1 cut(s) 629
SspMI CTAG 5 cut(s) 543, 953, 1101, 1497, 1508
SstI GAGCTC 1 cut(s) 553
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 108, 595
TaaI ACNGT 2 cut(s) 639, 1126
TaqI TCGA 8 cut(s) 24, 267, 322, 405, 724, 1128, 1286, 1551
TfiI GAWTC 7 cut(s) 178, 281, 402, 790, 998, 1288, 1418
Tru1I TTAA 3 cut(s) 51, 960, 1583
Tru9I TTAA 3 cut(s) 51, 960, 1583
TscAI CASTG 2 cut(s) 873, 1102
TseFI GTSAC 4 cut(s) 73, 1004, 1093, 1186
TseI GCWGC 4 cut(s) 488, 626, 848, 1205
Tsp45I GTSAC 4 cut(s) 73, 1004, 1093, 1186
TspGWI ACGGA 2 cut(s) 69, 644
TspRI CASTG 2 cut(s) 873, 1102
VpaK11BI GGWCC 4 cut(s) 56, 410, 557, 769
XapI RAATTY 4 cut(s) 230, 796, 816, 1037
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 900
XmnI GAANNNNTTC 2 cut(s) 820, 1458
XspI CTAG 5 cut(s) 543, 953, 1101, 1497, 1508
Zsp2I ATGCAT 2 cut(s) 227, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.