MD15G1285500.v1.1

Nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005674
Physical Location & Seq
Reverse (-)
26008592 .. 26014739
6148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1285500.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGAAGCGAATTCGAACGGCCCGATTGATTCGGGTCGGGTCATTGCGCCCCCCACCAATCCAATGGTCACCCCCCTGCTCACGGATCTCTACCAGTTCACCATGGCCTACGCTTACTGGAAAGCCGGCAAGCACAACGAACAAGCTGTGTTTGATTTGTATTTCCGGAAGAACCCCTTCGGCGGCGAATACACAATCTTTGCCGGTTTGGAAGAATGCATCAGATTCATTGCCAATTTCAAATTGACTGAGGATGATATCGGCTTCGTCCGCGAATCATTGTCTTCTTCGTGCGAGGATGGATTCTATGACTATCTTAGAGGAGTTGATTGTTCAGAAGTTAAAGTGTATGCCATTCCTGAGGGTACAGTTGTTTTTCCAAAGGTTCCCCTGATTCGAGTTGAAGGGCCAATTGCGGTGGTTCAATTGCTCGAAACTCCGTTTTTGAATCTTGTTAATTATGCATCTTTAGTTGCTACGAATGCTGCAAGGCATCGTTTTGTTGCTGGAGAATCAAAGAACCTACTTGAGTTTGGGCTTCGACGGGCTCAGGGACCTGATGGGGGAATAGGGGCATCAAGGTACTGTTACCTGGGAGGATTTCATGCAACAAGTAATGTTGCAGCTGGGAAGTTATTCGGGATACCACTTCGTGGAACCCATTCTCATGCTTTTGTCAGCTCATTTATGAGCCCTGACGAGATTATTGATAAATCACTTCATAGCAGTGATGGCCAGAGTACTTGTGAGGATTTTGTTGGCCTGGTTCAGACTTGGTTAAGCAAGATTCAGTGGTCAAAATCATTAAATGGAACTTTTGGCGAGACCAATCAAAGTGAGCTAGCAGCATTCACATCATATGCATTGGCATTCCCTAGTAACTTTCTAGCTCTTGTAGACACATATGATGTTATGAGGAGTGGAATCCCTAACTATTGTGCCGTTGCTTTAGCACTCAATGATTTAGGGTACAAAGCAGCCGGAATTAGGTTGGATTCAGGTGACCTAGCATATTTGTCATGCGAGGCTCGGAAGATTTTCTGCACCATTGAGAAAGAATTTGGAGTACCTTGGTTTGGAAAGACCAATATTACAGCTAGTAATGATCTGAACGAGGAAACTTTGGATGCTTTAAACAAACAGGGTCATGAGGTTGACTCATTTGGAATTGGAACCTATGTAGTTACTTGCTATGCTCAAGCTGCTTTAGGGTGCGTTTTCAAGCTTGTTGAGATAAATAATCAGCCTCGAATAAAACTTTCTGAAGATGTTACGAAGGTCTCCATTCCTTGTAAAAAGCGGAGTTACAGACTGTATGGGAAAGAAGGTTATCCCCTGGTAGATATAATGACAGGGGAAAATGAACCACCTCCAAAGGTGGGAGAAAGAATTCTATGTCGTCACCCTTTTAATGAATCAAAGAGAGCATATGTGGTGCCACAGCGCGTTGAAGAACTTATGAAATGTTTCTGGCCTGGCAGCCCAGGTGGAGTAAGAGAAGATCTGCCACCATTGAAGGACATTAGGGAGCGCTGCATTACACAACTCAAGAAAATGCGTCCCGACCACATGAGGAGACTAAACCCAACGCCGTACAAGGTTAGTGTGAGTGCGGAATTGTACGACTTCATTCATTTCTTGTGGCTCAACGAAGCACCTGTTGGGGAGTTGCAGTGA

Protein Analysis

559

Amino Acids

62.07

Weight (kDa)

5.81

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 26 - 153 1.8e-39 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 113 - 156 3.8e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 432 - 543 7.1e-32 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1435
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 897
AccII CGCG 2 cut(s) 273, 1446
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 5 cut(s) 183, 271, 416, 1300, 1613
AclWI GGATC 1 cut(s) 93
AcoI YGGCCR 1 cut(s) 733
AcsI RAATTY 3 cut(s) 10, 1058, 1389
AcuI CTGAAG 1 cut(s) 1284
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 7 cut(s) 367, 584, 742, 971, 1068, 1595, 1622
AfeI AGCGCT 1 cut(s) 1532
AfiI CCNNNNNNNGG 7 cut(s) 82, 182, 563, 653, 987, 1076, 1379
AgsI TTSAA 7 cut(s) 241, 404, 425, 448, 1222, 1451, 1516
AjnI CCWGG 5 cut(s) 591, 762, 1335, 1474, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 1644, 1676
AluBI AGCT 8 cut(s) 146, 626, 681, 841, 890, 1097, 1202, 1225
AluI AGCT 8 cut(s) 146, 626, 681, 841, 890, 1097, 1202, 1225
Alw26I GTCTC 3 cut(s) 818, 1285, 1570
AlwI GGATC 1 cut(s) 93
Aor13HI TCCGGA 1 cut(s) 165
Aor51HI AGCGCT 1 cut(s) 1532
AoxI GGCC 6 cut(s) 19, 105, 407, 733, 760, 1472
ApeKI GCWGC 7 cut(s) 485, 623, 845, 977, 1202, 1479, 1533
ApoI RAATTY 3 cut(s) 10, 1058, 1389
Asp700I GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1389, 1466
AspLEI GCGC 3 cut(s) 49, 1446, 1533
AspS9I GGNCC 3 cut(s) 20, 407, 554
AsuHPI GGTGA 4 cut(s) 61, 91, 1013, 1394
AsuII TTCGAA 1 cut(s) 14
AsuNHI GCTAGC 1 cut(s) 841
AvaII GGWCC 1 cut(s) 554
AxyI CCTNAGG 1 cut(s) 360
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BalI TGGCCA 1 cut(s) 735
BanI GGYRCC 1 cut(s) 1435
BanII GRGCYC 2 cut(s) 550, 695
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 7 cut(s) 472, 635, 857, 989, 1189, 1491, 1520
BccI CCATC 3 cut(s) 293, 554, 725
BceAI ACGGC 3 cut(s) 34, 926, 1576
BciT130I CCWGG 5 cut(s) 593, 764, 1337, 1476, 1485
BciVI GTATCC 1 cut(s) 636
BcoDI GTCTC 3 cut(s) 818, 1285, 1570
BfaI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
BfoI RGCGCY 1 cut(s) 1534
BfuI GTATCC 1 cut(s) 636
BglII AGATCT 1 cut(s) 1501
BisI GCNGC 8 cut(s) 184, 486, 624, 846, 978, 1203, 1480, 1534
BlsI GCNGC 8 cut(s) 185, 487, 625, 847, 979, 1204, 1481, 1535
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 5 cut(s) 593, 764, 1337, 1476, 1485
Bme18I GGWCC 1 cut(s) 554
BmgT120I GGNCC 3 cut(s) 20, 407, 554
BmiI GGNNCC 5 cut(s) 387, 555, 658, 1174, 1437
BmrFI CCNGG 5 cut(s) 593, 764, 1337, 1476, 1485
BmsI GCATC 5 cut(s) 228, 473, 502, 584, 1117
BmtI GCTAGC 1 cut(s) 845
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 1142, 1174
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 14
BpuEI CTTGAG 3 cut(s) 548, 1182, 1532
BsaI GGTCTC 2 cut(s) 818, 1285
BsaJI CCNNGG 5 cut(s) 102, 592, 1070, 1335, 1483
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 1 cut(s) 1658
Bsc4I CCNNNNNNNGG 7 cut(s) 82, 182, 563, 653, 987, 1076, 1379
Bse118I RCCGGY 2 cut(s) 125, 203
Bse1I ACTGG 2 cut(s) 94, 122
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 2 cut(s) 42, 228
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 5 cut(s) 593, 764, 1337, 1476, 1485
BseDI CCNNGG 5 cut(s) 102, 592, 1070, 1335, 1483
BseGI GGATG 3 cut(s) 259, 304, 1132
BseLI CCNNNNNNNGG 7 cut(s) 82, 182, 563, 653, 987, 1076, 1379
BseMI GCAATG 2 cut(s) 42, 228
BseMII CTCAG 3 cut(s) 240, 351, 563
BseNI ACTGG 2 cut(s) 94, 122
BseRI GAGGAG 3 cut(s) 336, 931, 1588
BseXI GCAGC 7 cut(s) 472, 635, 857, 989, 1189, 1491, 1520
BseYI CCCAGC 1 cut(s) 626
BsgI GTGCAG 1 cut(s) 1027
Bsh1236I CGCG 2 cut(s) 273, 1446
BshFI GGCC 6 cut(s) 21, 107, 409, 735, 762, 1474
BshNI GGYRCC 1 cut(s) 1435
BsiSI CCGG 4 cut(s) 126, 166, 204, 981
BslFI GGGAC 2 cut(s) 567, 1545
BslI CCNNNNNNNGG 7 cut(s) 82, 182, 563, 653, 987, 1076, 1379
BsmAI GTCTC 3 cut(s) 818, 1285, 1570
BsmFI GGGAC 2 cut(s) 567, 1545
BsmI GAATGC 4 cut(s) 221, 487, 848, 869
BsnI GGCC 6 cut(s) 21, 107, 409, 735, 762, 1474
Bso31I GGTCTC 2 cut(s) 818, 1285
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 550, 695
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 85, 1105, 1501
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 5 cut(s) 183, 271, 416, 1300, 1613
BspANI GGCC 6 cut(s) 21, 107, 409, 735, 762, 1474
BspCNI CTCAG 3 cut(s) 241, 352, 562
BspEI TCCGGA 1 cut(s) 165
BspFNI CGCG 2 cut(s) 273, 1446
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 5 cut(s) 387, 555, 658, 1174, 1437
BspOI GCTAGC 1 cut(s) 845
BspPI GGATC 1 cut(s) 93
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 1435
BspTNI GGTCTC 2 cut(s) 818, 1285
BsrDI GCAATG 2 cut(s) 42, 228
BsrFI RCCGGY 2 cut(s) 125, 203
BsrI ACTGG 2 cut(s) 94, 122
BssAI RCCGGY 2 cut(s) 125, 203
BssECI CCNNGG 5 cut(s) 102, 592, 1070, 1335, 1483
BssMI GATC 3 cut(s) 85, 1105, 1501
BssT1I CCWWGG 2 cut(s) 102, 1070
Bst2UI CCWGG 5 cut(s) 593, 764, 1337, 1476, 1485
Bst4CI ACNGT 3 cut(s) 370, 587, 1314
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 127, 131, 843
BstDEI CTNAG 4 cut(s) 249, 317, 360, 549
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 2 cut(s) 67, 1001
BstF5I GGATG 3 cut(s) 259, 304, 1132
BstFNI CGCG 2 cut(s) 273, 1446
BstH2I RGCGCY 1 cut(s) 1534
BstHHI GCGC 3 cut(s) 49, 1446, 1533
BstKTI GATC 3 cut(s) 88, 1108, 1504
BstMAI GTCTC 3 cut(s) 818, 1285, 1570
BstMBI GATC 3 cut(s) 85, 1105, 1501
BstMWI GCNNNNNNNGC 4 cut(s) 270, 482, 732, 1202
BstNI CCWGG 5 cut(s) 593, 764, 1337, 1476, 1485
BstPI GGTNACC 2 cut(s) 67, 1001
BstSCI CCNGG 5 cut(s) 591, 762, 1335, 1474, 1483
BstUI CGCG 2 cut(s) 273, 1446
BstV1I GCAGC 7 cut(s) 472, 635, 857, 989, 1189, 1491, 1520
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 85, 1501
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 2 cut(s) 85, 1501
Bsu36I CCTNAGG 1 cut(s) 360
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 6 cut(s) 21, 107, 409, 735, 762, 1474
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 259, 304, 1132
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 2 cut(s) 733, 797
Cac8I GCNNGC 3 cut(s) 127, 131, 843
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 3 cut(s) 49, 1446, 1533
Cfr10I RCCGGY 2 cut(s) 125, 203
Cfr13I GGNCC 3 cut(s) 20, 407, 554
CseI GACGC 1 cut(s) 1547
Csp6I GTAC 7 cut(s) 366, 583, 741, 970, 1067, 1594, 1621
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
CviAII CATG 6 cut(s) 103, 605, 668, 1020, 1148, 1570
CviQI GTAC 7 cut(s) 366, 583, 741, 970, 1067, 1594, 1621
DdeI CTNAG 4 cut(s) 249, 317, 360, 549
DpnI GATC 3 cut(s) 87, 1107, 1503
DpnII GATC 3 cut(s) 85, 1105, 1501
DraI TTTAAA 1 cut(s) 1134
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 1 cut(s) 733
Eco130I CCWWGG 2 cut(s) 102, 1070
Eco24I GRGCYC 2 cut(s) 550, 695
Eco31I GGTCTC 2 cut(s) 818, 1285
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 554
Eco47III AGCGCT 1 cut(s) 1532
Eco57I CTGAAG 1 cut(s) 1284
Eco81I CCTNAGG 1 cut(s) 360
Eco91I GGTNACC 2 cut(s) 67, 1001
EcoO109I RGGNCCY 1 cut(s) 554
EcoO65I GGTNACC 2 cut(s) 67, 1001
EcoRI GAATTC 2 cut(s) 10, 1389
EcoRII CCWGG 5 cut(s) 591, 762, 1335, 1474, 1483
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 102, 1070
EcoT22I ATGCAT 3 cut(s) 221, 466, 865
EcoT38I GRGCYC 2 cut(s) 550, 695
ErhI CCWWGG 2 cut(s) 102, 1070
FaeI CATG 6 cut(s) 106, 608, 671, 1023, 1151, 1573
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 2 cut(s) 567, 1545
FatI CATG 6 cut(s) 102, 604, 667, 1019, 1147, 1569
FauNDI CATATG 3 cut(s) 859, 904, 1429
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 8 cut(s) 184, 486, 624, 846, 978, 1203, 1480, 1534
FokI GGATG 3 cut(s) 266, 311, 1139
FriOI GRGCYC 2 cut(s) 550, 695
Fsp4HI GCNGC 8 cut(s) 184, 486, 624, 846, 978, 1203, 1480, 1534
FspBI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
GlaI GCGC 3 cut(s) 48, 1445, 1532
GluI GCNGC 8 cut(s) 184, 486, 624, 846, 978, 1203, 1480, 1534
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 1 cut(s) 1534
HaeIII GGCC 6 cut(s) 21, 107, 409, 735, 762, 1474
HapII CCGG 4 cut(s) 126, 166, 204, 981
HgaI GACGC 1 cut(s) 1547
HhaI GCGC 3 cut(s) 49, 1446, 1533
Hin1II CATG 6 cut(s) 106, 608, 671, 1023, 1151, 1573
Hin6I GCGC 3 cut(s) 47, 1444, 1531
HinP1I GCGC 3 cut(s) 47, 1444, 1531
HincII GTYRAC 1 cut(s) 1156
HindII GTYRAC 1 cut(s) 1156
HindIII AAGCTT 1 cut(s) 1223
HpaII CCGG 4 cut(s) 126, 166, 204, 981
HphI GGTGA 4 cut(s) 61, 91, 1013, 1394
Hpy166II GTNNAC 3 cut(s) 99, 898, 1156
Hpy188I TCNGA 6 cut(s) 224, 337, 771, 1032, 1110, 1264
Hpy188III TCNNGA 6 cut(s) 166, 359, 640, 1148, 1549, 1562
Hpy8I GTNNAC 3 cut(s) 99, 898, 1156
Hpy99I CGWCG 1 cut(s) 546
HpyAV CCTTC 5 cut(s) 187, 398, 1270, 1319, 1510
HpyCH4III ACNGT 3 cut(s) 370, 587, 1314
HpyCH4V TGCA 9 cut(s) 219, 464, 488, 608, 623, 863, 1044, 1536, 1672
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 482, 732, 1202
HpyF3I CTNAG 4 cut(s) 249, 317, 360, 549
Hsp92II CATG 6 cut(s) 106, 608, 671, 1023, 1151, 1573
HspAI GCGC 3 cut(s) 47, 1444, 1531
Kpn2I TCCGGA 1 cut(s) 165
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 85, 1105, 1501
LmnI GCTCC 1 cut(s) 1528
Lsp1109I GCAGC 7 cut(s) 472, 635, 857, 989, 1189, 1491, 1520
LweI GCATC 5 cut(s) 228, 473, 502, 584, 1117
MaeI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
MaeIII GTNAC 8 cut(s) 67, 587, 878, 1001, 1183, 1270, 1304, 1400
MalI GATC 3 cut(s) 87, 1107, 1503
MboI GATC 3 cut(s) 85, 1105, 1501
MboII GAAGA 8 cut(s) 181, 224, 276, 279, 1045, 1277, 1463, 1511
MfeI CAATTG 2 cut(s) 411, 425
MflI RGATCY 2 cut(s) 85, 1501
MhlI GDGCHC 2 cut(s) 550, 695
MlsI TGGCCA 1 cut(s) 735
MluNI TGGCCA 1 cut(s) 735
MlyI GAGTC 1 cut(s) 1151
MmeI TCCRAC 1 cut(s) 972
Mox20I TGGCCA 1 cut(s) 735
Mph1103I ATGCAT 3 cut(s) 221, 466, 865
MroI TCCGGA 1 cut(s) 165
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1389, 1466
MscI TGGCCA 1 cut(s) 735
MseI TTAA 6 cut(s) 342, 456, 779, 806, 1133, 1410
MslI CAYNNNNRTG 2 cut(s) 666, 726
Msp20I TGGCCA 1 cut(s) 735
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 4 cut(s) 126, 166, 204, 981
MspR9I CCNGG 5 cut(s) 593, 764, 1337, 1476, 1485
MunI CAATTG 2 cut(s) 411, 425
Mva1269I GAATGC 4 cut(s) 221, 487, 848, 869
MvaI CCWGG 5 cut(s) 593, 764, 1337, 1476, 1485
MvnI CGCG 2 cut(s) 273, 1446
MwoI GCNNNNNNNGC 4 cut(s) 270, 482, 732, 1202
NaeI GCCGGC 1 cut(s) 127
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 3 cut(s) 859, 904, 1429
NdeII GATC 3 cut(s) 85, 1105, 1501
NgoMIV GCCGGC 1 cut(s) 125
NheI GCTAGC 1 cut(s) 841
NlaIII CATG 6 cut(s) 106, 608, 671, 1023, 1151, 1573
NlaIV GGNNCC 5 cut(s) 387, 555, 658, 1174, 1437
NmuCI GTSAC 3 cut(s) 67, 1001, 1400
NsiI ATGCAT 3 cut(s) 221, 466, 865
NspV TTCGAA 1 cut(s) 14
PagI TCATGA 1 cut(s) 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 4 cut(s) 221, 487, 848, 869
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1389, 1466
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 8 cut(s) 185, 487, 625, 847, 979, 1204, 1481, 1535
PleI GAGTC 1 cut(s) 1151
PpsI GAGTC 1 cut(s) 1151
PpuMI RGGWCCY 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 554
Psp6I CCWGG 5 cut(s) 591, 762, 1335, 1474, 1483
PspEI GGTNACC 2 cut(s) 67, 1001
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 5 cut(s) 591, 762, 1335, 1474, 1483
PspN4I GGNNCC 5 cut(s) 387, 555, 658, 1174, 1437
PspPI GGNCC 3 cut(s) 20, 407, 554
PspPPI RGGWCCY 1 cut(s) 554
PsuI RGATCY 2 cut(s) 85, 1501
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 7 cut(s) 367, 584, 742, 971, 1068, 1595, 1622
RsaNI GTAC 7 cut(s) 366, 583, 741, 970, 1067, 1594, 1621
RseI CAYNNNNRTG 2 cut(s) 666, 726
SaqAI TTAA 6 cut(s) 342, 456, 779, 806, 1133, 1410
SatI GCNGC 8 cut(s) 184, 486, 624, 846, 978, 1203, 1480, 1534
Sau3AI GATC 3 cut(s) 85, 1105, 1501
Sau96I GGNCC 3 cut(s) 20, 407, 554
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 1 cut(s) 1151
ScrFI CCNGG 5 cut(s) 593, 764, 1337, 1476, 1485
SduI GDGCHC 2 cut(s) 550, 695
SfaNI GCATC 5 cut(s) 228, 473, 502, 584, 1117
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 1 cut(s) 554
SmiMI CAYNNNNRTG 2 cut(s) 666, 726
SmlI CTYRAG 3 cut(s) 527, 1197, 1547
SmoI CTYRAG 3 cut(s) 527, 1197, 1547
SsiI CCGC 5 cut(s) 183, 271, 416, 1300, 1613
SspI AATATT 1 cut(s) 1090
SspMI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
StyD4I CCNGG 5 cut(s) 591, 762, 1335, 1474, 1483
StyI CCWWGG 2 cut(s) 102, 1070
TaaI ACNGT 3 cut(s) 370, 587, 1314
TaqI TCGA 5 cut(s) 14, 397, 432, 541, 1249
TatI WGTACW 1 cut(s) 740
TauI GCSGC 1 cut(s) 186
Tru1I TTAA 6 cut(s) 342, 456, 779, 806, 1133, 1410
Tru9I TTAA 6 cut(s) 342, 456, 779, 806, 1133, 1410
TscAI CASTG 2 cut(s) 733, 797
TseFI GTSAC 3 cut(s) 67, 1001, 1400
TseI GCWGC 7 cut(s) 485, 623, 845, 977, 1202, 1479, 1533
Tsp45I GTSAC 3 cut(s) 67, 1001, 1400
TspDTI ATGAA 8 cut(s) 217, 593, 710, 1377, 1428, 1475, 1618, 1622
TspGWI ACGGA 2 cut(s) 98, 429
TspRI CASTG 2 cut(s) 733, 797
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 554
XapI RAATTY 3 cut(s) 10, 1058, 1389
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1389, 1466
XspI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
ZrmI AGTACT 1 cut(s) 742
Zsp2I ATGCAT 3 cut(s) 221, 466, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.