Rh7CG469100

Nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0005674
Physical Location & Seq
Reverse (-)
62816455 .. 62818131
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG469100.1

Sequence Viewer

Length: 1677 bp
ATGGCGGCCATATCCAACGGCCAGGTCCTCGCCGGCCCCACCAATCCAATGGTCACGCCTCTCCTCACCGATCTCTACCAGTTCACCATGGCCTACGCTTACTGGAAAGCCGGCAAGCACAAGGAACGAGCCGTGTTTGATTTGTATTTCCGCAAGAATCCATTTGGCGGCGAATACACAGTGTTTGCTGGATTAGAAGAATGCATCAAATTCATTGCTAATATCAAATTGGCCGACGACGAAATCGATTTCGTCCGCCAATCCTTGTCTTCTTCATGTGAGCTAGAGGAAGGATTCTATAAGTATCTGAGTGGAGTTGACTGTTCCGAAGTTGAAGTCTATGCTATCCAAGAAGGATCAGTCGTTTTTCCAAAGGTTCCCCTCGTTAGAGTTGAAGGACCAATCGCTGTGGTTCAGTTACTCGAAACTCCATTCTTGAATCTCATTAATTATGCGTCATTAGTTGCTACAAATGCCGCAAGGCATCGTTTCGTTGCTGGAGAATCTAAAATGCTACTTGAGTTTGGGCTTCGACCAGCTCAGGGACCTGATGGTGGAATAGGGGCATCTAGGTACTGCTACATGGGAGGATTTCATGCAACAAGTAATGTTGCCGCTGGAAAGATATTTGGGATCCCGCTTCGTGGAACACATTCACATGCTTTTGTCAGCTCATACATGAGCCCAGACGAGATTATAGATAAATCACTTCATCATAGCAGTGATAAGCGCAGTACTTGTGAGGATTTTGTTAGCCTGGTTCAGACTTGGTTGAGCAAAATTCAGTCGTGGTCAAATTCATTAAATAATGGTACTTTTTTTGGTGAGACCAATCATAGTGAGCTTGCAGCATTCACATCGTATGCCCTGGCATTCCCTGATAACTTTCTAGCTCTTGTAGACACATATGATGTTATGAGGAGTGGAATTCCCAACTTTTGTGCAGTTGCTTTAGCACTCAATGATCTAGGGTACAAACTAGCTGGCATTAGGTTGGATTCAGGTGATCTAGCATATCTCTCCTCTGAGGCTCGAAAGATTTTTCTCATCATTGAGAAAGAATTTGGAGTAGCTGGATTTGGAAAGACGGTTATCACAGCTAGTAATGATCTAAATGAGGAAACTTTGGATGCTTTAAACAAACAGGGCCATGAGGTTGATGCATTTGGAATTGGGACCCATGTAGTTACTTGTTATGCTCAAGCTGCTCTGGGTTGTGTTTTCAAGCTTGTTGAGATAAATAATCAGCCTCGCATCAAACTTTCTGAAGATGTCACAAAGGTCTCCATCCCTTGTAAAAAACGATCTTACAGATTGTTTGGGAAAGAAGGTTATCCCCTGGTAGACATAATGACCGGAGAAAATGAACCACCTCCAAAGGTTGGAGAACGGATCCTGTGTCGTCACCCCTTTAATGAATCGAAGAGAGCATATGTAGTGCCGCAGCGTGTTGAGGAACTTATGAAATGCTTTTGGCATGGTCCAGGTGGAGTGAGAGAAGATCTGCCACCATTAAAGGACATCAGGGAGCGCTGCATCAGCCAACTCGGGAAAATGCGTCCCGACCACATGAGGAGACTAAACCCCACTCCGTATAAGGTTAGTGTAAGTGCAAAACTGTATGACTTCATTCACTTCCTATGGCTCAATGAGGCACCTGTCGGGGAGTTGCAGTGA

Protein Analysis

558

Amino Acids

61.99

Weight (kDa)

6.1

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 21 - 150 1.6e-37 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 110 - 153 5.9e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 433 - 543 1.5e-31 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1654
AccB7I CCANNNNNTGG 1 cut(s) 1382
AccI GTMKAC 2 cut(s) 900, 1344
AciI CCGC 8 cut(s) 5, 151, 168, 256, 477, 615, 638, 1442
AclWI GGATC 5 cut(s) 364, 628, 641, 1387, 1400
AcoI YGGCCR 3 cut(s) 6, 19, 231
AcsI RAATTY 5 cut(s) 209, 780, 796, 927, 1061
AcuI CTGAAG 1 cut(s) 1287
AfaI GTAC 4 cut(s) 575, 736, 814, 974
AfeI AGCGCT 1 cut(s) 1532
AfiI CCNNNNNNNGG 5 cut(s) 167, 542, 554, 644, 1382
AgsI TTSAA 4 cut(s) 335, 395, 439, 1225
AjnI CCWGG 5 cut(s) 21, 756, 867, 1338, 1483
Alw26I GTCTC 3 cut(s) 821, 1288, 1570
AlwI GGATC 5 cut(s) 364, 628, 641, 1387, 1400
Ama87I CYCGRG 1 cut(s) 1547
Aor51HI AGCGCT 1 cut(s) 1532
AoxI GGCC 6 cut(s) 6, 19, 34, 90, 231, 1147
ApeKI GCWGC 4 cut(s) 848, 1205, 1444, 1533
ApoI RAATTY 5 cut(s) 209, 780, 796, 927, 1061
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AseI ATTAAT 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 652
AspLEI GCGC 2 cut(s) 732, 1533
AspS9I GGNCC 7 cut(s) 25, 35, 398, 545, 1147, 1176, 1481
AsuHPI GGTGA 5 cut(s) 58, 76, 836, 1016, 1397
AvaI CYCGRG 1 cut(s) 1547
AvaII GGWCC 5 cut(s) 25, 398, 545, 1176, 1481
BamHI GGATCC 2 cut(s) 633, 1392
BanI GGYRCC 1 cut(s) 1654
BanII GRGCYC 1 cut(s) 686
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 860, 1192, 1456, 1520
BccI CCATC 2 cut(s) 545, 1295
BceAI ACGGC 2 cut(s) 34, 116
BcgI CGANNNNNNTGC 2 cut(s) 840, 874
BciT130I CCWGG 5 cut(s) 23, 758, 869, 1340, 1485
BcoDI GTCTC 3 cut(s) 821, 1288, 1570
BfaI CTAG 7 cut(s) 284, 570, 890, 968, 980, 1010, 1101
BfoI RGCGCY 1 cut(s) 1534
BglII AGATCT 1 cut(s) 1501
BisI GCNGC 9 cut(s) 6, 169, 477, 615, 849, 1206, 1442, 1445, 1534
BlsI GCNGC 9 cut(s) 7, 170, 478, 616, 850, 1207, 1443, 1446, 1535
BmcAI AGTACT 1 cut(s) 736
Bme1390I CCNGG 5 cut(s) 23, 758, 869, 1340, 1485
Bme18I GGWCC 5 cut(s) 25, 398, 545, 1176, 1481
BmeT110I CYCGRG 1 cut(s) 1547
BmgT120I GGNCC 7 cut(s) 25, 35, 398, 545, 1147, 1176, 1481
BmiI GGNNCC 8 cut(s) 37, 378, 546, 635, 1177, 1178, 1394, 1656
BmrFI CCNGG 5 cut(s) 23, 758, 869, 1340, 1485
BmsI GCATC 7 cut(s) 213, 493, 575, 1120, 1150, 1263, 1545
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 2 cut(s) 539, 1185
Bsa29I ATCGAT 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 821, 1288
BsaJI CCNNGG 3 cut(s) 87, 867, 1338
BsaWI WCCGGW 1 cut(s) 1355
BsaXI ACNNNNNCTCC 1 cut(s) 1658
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 542, 554, 644, 1382
Bse118I RCCGGY 2 cut(s) 32, 110
Bse1I ACTGG 2 cut(s) 79, 107
Bse3DI GCAATG 1 cut(s) 213
BseBI CCWGG 5 cut(s) 23, 758, 869, 1340, 1485
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 87, 867, 1338
BseGI GGATG 2 cut(s) 1135, 1287
BseLI CCNNNNNNNGG 5 cut(s) 167, 542, 554, 644, 1382
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 3 cut(s) 299, 554, 1017
BseNI ACTGG 2 cut(s) 79, 107
BseRI GAGGAG 4 cut(s) 53, 934, 1012, 1588
BseXI GCAGC 4 cut(s) 860, 1192, 1456, 1520
BsgI GTGCAG 1 cut(s) 963
BshFI GGCC 6 cut(s) 8, 21, 36, 92, 233, 1149
BshNI GGYRCC 1 cut(s) 1654
BshVI ATCGAT 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 1547
BsiSI CCGG 3 cut(s) 33, 111, 1356
BslFI GGGAC 3 cut(s) 558, 1189, 1545
BslI CCNNNNNNNGG 5 cut(s) 167, 542, 554, 644, 1382
BsmAI GTCTC 3 cut(s) 821, 1288, 1570
BsmFI GGGAC 3 cut(s) 558, 1189, 1545
BsmI GAATGC 3 cut(s) 206, 851, 872
BsnI GGCC 6 cut(s) 8, 21, 36, 92, 233, 1149
Bso31I GGTCTC 2 cut(s) 821, 1288
BsoBI CYCGRG 1 cut(s) 1547
Bsp1286I GDGCHC 1 cut(s) 686
Bsp143I GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 8 cut(s) 5, 151, 168, 256, 477, 615, 638, 1442
BspANI GGCC 6 cut(s) 8, 21, 36, 92, 233, 1149
BspCNI CTCAG 3 cut(s) 300, 553, 1018
BspDI ATCGAT 1 cut(s) 246
BspLI GGNNCC 8 cut(s) 37, 378, 546, 635, 1177, 1178, 1394, 1656
BspPI GGATC 5 cut(s) 364, 628, 641, 1387, 1400
BspT107I GGYRCC 1 cut(s) 1654
BspTNI GGTCTC 2 cut(s) 821, 1288
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 2 cut(s) 32, 110
BsrI ACTGG 2 cut(s) 79, 107
BssAI RCCGGY 2 cut(s) 32, 110
BssECI CCNNGG 3 cut(s) 87, 867, 1338
BssMI GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 5 cut(s) 23, 758, 869, 1340, 1485
Bst4CI ACNGT 4 cut(s) 181, 323, 1090, 1620
Bst6I CTCTTC 1 cut(s) 1418
BstC8I GCNNGC 5 cut(s) 34, 112, 116, 846, 985
BstDEI CTNAG 3 cut(s) 308, 540, 1026
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 1135, 1287
BstH2I RGCGCY 1 cut(s) 1534
BstHHI GCGC 2 cut(s) 732, 1533
BstKTI GATC 9 cut(s) 73, 359, 636, 967, 1009, 1111, 1307, 1395, 1504
BstMAI GTCTC 3 cut(s) 821, 1288, 1570
BstMBI GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 473, 1205, 1539
BstNI CCWGG 5 cut(s) 23, 758, 869, 1340, 1485
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 5 cut(s) 21, 756, 867, 1338, 1483
BstV1I GCAGC 4 cut(s) 860, 1192, 1456, 1520
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 3 cut(s) 633, 1392, 1501
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 3 cut(s) 633, 1392, 1501
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 6 cut(s) 8, 21, 36, 92, 233, 1149
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 1135, 1287
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 2 cut(s) 186, 727
Cac8I GCNNGC 5 cut(s) 34, 112, 116, 846, 985
CfoI GCGC 2 cut(s) 732, 1533
Cfr10I RCCGGY 2 cut(s) 32, 110
Cfr13I GGNCC 7 cut(s) 25, 35, 398, 545, 1147, 1176, 1481
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 2 cut(s) 444, 1547
Csp6I GTAC 4 cut(s) 574, 735, 813, 973
CspCI CAANNNNNGTGG 2 cut(s) 390, 425
CviQI GTAC 4 cut(s) 574, 735, 813, 973
DdeI CTNAG 3 cut(s) 308, 540, 1026
DpnI GATC 9 cut(s) 72, 358, 635, 966, 1008, 1110, 1306, 1394, 1503
DpnII GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
DraI TTTAAA 1 cut(s) 1137
EaeI YGGCCR 3 cut(s) 6, 19, 231
Eam1104I CTCTTC 1 cut(s) 1418
EarI CTCTTC 1 cut(s) 1418
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 686
Eco31I GGTCTC 2 cut(s) 821, 1288
Eco47I GGWCC 5 cut(s) 25, 398, 545, 1176, 1481
Eco47III AGCGCT 1 cut(s) 1532
Eco57I CTGAAG 1 cut(s) 1287
Eco88I CYCGRG 1 cut(s) 1547
EcoO109I RGGNCCY 3 cut(s) 25, 545, 1176
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 5 cut(s) 21, 756, 867, 1338, 1483
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 2 cut(s) 206, 1165
EcoT38I GRGCYC 1 cut(s) 686
ErhI CCWWGG 1 cut(s) 87
FaqI GGGAC 3 cut(s) 558, 1189, 1545
FauI CCCGC 1 cut(s) 645
FauNDI CATATG 2 cut(s) 907, 1432
FblI GTMKAC 2 cut(s) 900, 1344
Fnu4HI GCNGC 9 cut(s) 6, 169, 477, 615, 849, 1206, 1442, 1445, 1534
FokI GGATG 2 cut(s) 1142, 1274
FriOI GRGCYC 1 cut(s) 686
Fsp4HI GCNGC 9 cut(s) 6, 169, 477, 615, 849, 1206, 1442, 1445, 1534
FspBI CTAG 7 cut(s) 284, 570, 890, 968, 980, 1010, 1101
GlaI GCGC 2 cut(s) 731, 1532
GluI GCNGC 9 cut(s) 6, 169, 477, 615, 849, 1206, 1442, 1445, 1534
GsuI CTGGAG 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 1534
HaeIII GGCC 6 cut(s) 8, 21, 36, 92, 233, 1149
HapII CCGG 3 cut(s) 33, 111, 1356
HgaI GACGC 2 cut(s) 444, 1547
HhaI GCGC 2 cut(s) 732, 1533
Hin6I GCGC 2 cut(s) 730, 1531
HinP1I GCGC 2 cut(s) 730, 1531
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 1226
HinfI GANTC 6 cut(s) 157, 294, 439, 503, 998, 1418
HpaII CCGG 3 cut(s) 33, 111, 1356
HphI GGTGA 5 cut(s) 58, 76, 836, 1016, 1397
Hpy166II GTNNAC 4 cut(s) 84, 319, 901, 1345
Hpy188I TCNGA 5 cut(s) 309, 328, 765, 1027, 1267
Hpy188III TCNNGA 3 cut(s) 436, 1549, 1562
Hpy8I GTNNAC 4 cut(s) 84, 319, 901, 1345
Hpy99I CGWCG 2 cut(s) 239, 242
HpyAV CCTTC 4 cut(s) 284, 347, 389, 1322
HpyCH4III ACNGT 4 cut(s) 181, 323, 1090, 1620
HpyCH4V TGCA 8 cut(s) 204, 599, 848, 944, 1163, 1536, 1613, 1672
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 1205, 1539
HpyF3I CTNAG 3 cut(s) 308, 540, 1026
HspAI GCGC 2 cut(s) 730, 1531
KflI GGGWCCC 1 cut(s) 1176
KroI GCCGGC 2 cut(s) 32, 110
KroNI GCCGGC 2 cut(s) 34, 112
Kzo9I GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
LmnI GCTCC 1 cut(s) 1528
Lsp1109I GCAGC 4 cut(s) 860, 1192, 1456, 1520
LweI GCATC 7 cut(s) 213, 493, 575, 1120, 1150, 1263, 1545
MaeI CTAG 7 cut(s) 284, 570, 890, 968, 980, 1010, 1101
MaeIII GTNAC 5 cut(s) 52, 417, 1186, 1273, 1403
MalI GATC 9 cut(s) 72, 358, 635, 966, 1008, 1110, 1306, 1394, 1503
MboI GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
MboII GAAGA 6 cut(s) 209, 261, 264, 1280, 1435, 1511
MflI RGATCY 3 cut(s) 633, 1392, 1501
MhlI GDGCHC 1 cut(s) 686
MluCI AATT 8 cut(s) 209, 227, 448, 780, 796, 927, 1061, 1170
MmeI TCCRAC 3 cut(s) 39, 975, 1363
Mph1103I ATGCAT 2 cut(s) 206, 1165
MroNI GCCGGC 2 cut(s) 32, 110
MroXI GAANNNNTTC 1 cut(s) 652
MseI TTAA 5 cut(s) 447, 803, 1136, 1413, 1514
MslI CAYNNNNRTG 2 cut(s) 657, 720
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 3 cut(s) 33, 111, 1356
MspR9I CCNGG 5 cut(s) 23, 758, 869, 1340, 1485
Mva1269I GAATGC 3 cut(s) 206, 851, 872
MvaI CCWGG 5 cut(s) 23, 758, 869, 1340, 1485
MwoI GCNNNNNNNGC 3 cut(s) 473, 1205, 1539
NaeI GCCGGC 2 cut(s) 34, 112
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 2 cut(s) 907, 1432
NdeII GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
NgoMIV GCCGGC 2 cut(s) 32, 110
NlaIV GGNNCC 8 cut(s) 37, 378, 546, 635, 1177, 1178, 1394, 1656
NmuCI GTSAC 3 cut(s) 52, 1273, 1403
NsiI ATGCAT 2 cut(s) 206, 1165
NspI RCATGY 1 cut(s) 662
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 3 cut(s) 206, 851, 872
PdiI GCCGGC 2 cut(s) 34, 112
PdmI GAANNNNTTC 1 cut(s) 652
PfeI GAWTC 6 cut(s) 157, 294, 439, 503, 998, 1418
PflMI CCANNNNNTGG 1 cut(s) 1382
PkrI GCNGC 9 cut(s) 7, 170, 478, 616, 850, 1207, 1443, 1446, 1535
PpuMI RGGWCCY 3 cut(s) 25, 545, 1176
PshBI ATTAAT 1 cut(s) 447
Psp5II RGGWCCY 3 cut(s) 25, 545, 1176
Psp6I CCWGG 5 cut(s) 21, 756, 867, 1338, 1483
PspGI CCWGG 5 cut(s) 21, 756, 867, 1338, 1483
PspN4I GGNNCC 8 cut(s) 37, 378, 546, 635, 1177, 1178, 1394, 1656
PspPI GGNCC 7 cut(s) 25, 35, 398, 545, 1147, 1176, 1481
PspPPI RGGWCCY 3 cut(s) 25, 545, 1176
PsuI RGATCY 3 cut(s) 633, 1392, 1501
RsaI GTAC 4 cut(s) 575, 736, 814, 974
RsaNI GTAC 4 cut(s) 574, 735, 813, 973
RseI CAYNNNNRTG 2 cut(s) 657, 720
SaqAI TTAA 5 cut(s) 447, 803, 1136, 1413, 1514
SatI GCNGC 9 cut(s) 6, 169, 477, 615, 849, 1206, 1442, 1445, 1534
Sau3AI GATC 9 cut(s) 70, 356, 633, 964, 1006, 1108, 1304, 1392, 1501
Sau96I GGNCC 7 cut(s) 25, 35, 398, 545, 1147, 1176, 1481
ScaI AGTACT 1 cut(s) 736
ScrFI CCNGG 5 cut(s) 23, 758, 869, 1340, 1485
SduI GDGCHC 1 cut(s) 686
SfaNI GCATC 7 cut(s) 213, 493, 575, 1120, 1150, 1263, 1545
SinI GGWCC 5 cut(s) 25, 398, 545, 1176, 1481
SmiMI CAYNNNNRTG 2 cut(s) 657, 720
SmlI CTYRAG 2 cut(s) 518, 1200
SmoI CTYRAG 2 cut(s) 518, 1200
Sse9I AATT 8 cut(s) 209, 227, 448, 780, 796, 927, 1061, 1170
SsiI CCGC 8 cut(s) 5, 151, 168, 256, 477, 615, 638, 1442
SspMI CTAG 7 cut(s) 284, 570, 890, 968, 980, 1010, 1101
StyD4I CCNGG 5 cut(s) 21, 756, 867, 1338, 1483
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 181, 323, 1090, 1620
TaqI TCGA 5 cut(s) 246, 423, 532, 1033, 1421
TasI AATT 8 cut(s) 209, 227, 448, 780, 796, 927, 1061, 1170
TatI WGTACW 1 cut(s) 734
TauI GCSGC 5 cut(s) 8, 171, 479, 617, 1444
TfiI GAWTC 6 cut(s) 157, 294, 439, 503, 998, 1418
Tru1I TTAA 5 cut(s) 447, 803, 1136, 1413, 1514
Tru9I TTAA 5 cut(s) 447, 803, 1136, 1413, 1514
TscAI CASTG 2 cut(s) 186, 727
TseFI GTSAC 3 cut(s) 52, 1273, 1403
TseI GCWGC 4 cut(s) 848, 1205, 1444, 1533
Tsp45I GTSAC 3 cut(s) 52, 1273, 1403
TspDTI ATGAA 9 cut(s) 202, 264, 584, 701, 789, 1380, 1431, 1478, 1618
TspGWI ACGGA 2 cut(s) 1405, 1581
TspRI CASTG 2 cut(s) 186, 727
Van91I CCANNNNNTGG 1 cut(s) 1382
VpaK11BI GGWCC 5 cut(s) 25, 398, 545, 1176, 1481
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 5 cut(s) 209, 780, 796, 927, 1061
XceI RCATGY 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 900, 1344
XmnI GAANNNNTTC 1 cut(s) 652
XspI CTAG 7 cut(s) 284, 570, 890, 968, 980, 1010, 1101
ZrmI AGTACT 1 cut(s) 736
Zsp2I ATGCAT 2 cut(s) 206, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.