MD02G1174500.v1.1

Nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005674
Physical Location & Seq
Reverse (-)
15330527 .. 15336289
5763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1174500.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGCGGCGAAATCGAACGGCCCGATTGATACGGGGAGGGTCATTCCGGGCCCCACCAATCCAATGGTCACGCCCCTGCTCACGGATCTCTACCAGTTCACCATGGCCTACGCTTACTGGAAAGCCGGCAAGCACAACGAACGAGCCGTGTTTGATTTGTATTTCCGGAAGAACCCCTTCGGCGGCGAATACACAATCTTTGCCGGTTTGGAAGAATGCATCAGATTCATTGCCAATTTCAAATTGACTGAGGATGAAATTGCTTTCGTCCGCGAATCGTTGTCTTCTTCGTGCGAGGATGGATTCTATGACTATCTTAGAGGAGTTGATTGTTTGGGAGTTGAAGTGTATGCCATTGCTGAGGGTACAGTTGTTTTTCCAAAGGTTCCCCTGATTCGAGTCGAAGGGCCAATTGCGGTGGTTCAATTGCTCGAAACTCCCTTTTTGAATCTTGTTAATTATGCATCTTTAGTTGCTACGAATGCTGCAAGGCATCGTTTTGTTGCTGGAGAATCGAAGAATCTGCTTGAGTTTGGGCTTCGACGGGCTCAGGGACCTGATGGGGGCATAGCGGCATCAAGGTACTGCTACCTGGGAGGATTTCATGCAACAAGTAATGTTGCAGCTGGGAAGTTATTCGGGATACCACTTCGTGGAACCCATTCTCATGCTTTTGTCAGCTCATTTGTGAGCCCTGACGAGATTATTGATAAAACACTTCGTAGTAGTGATGGCCAGAGTACTTGTGAGGATTTTGTTAGCCTGGTTCAGACTTGGTTAAGCAAGATTAAGTGGTCAAAATCATTAAATGGCACTTTTGGCGAGACCAATCAAAGTGAACTAGCAGCATTCACATCATATGCATTGGCATTCCCTAGTAACTTTCTAGCTCTTGTAGACACATATGATGTTATGAGGAGTGGAATCCCTAACTATTGTGCCGTTGCTTTAGCACTCAATGATTTAGGGTACAAAGCAAGCGGAATTAGGTTGGATTCAGGTGACCTAGCATATTTGTCTTGCGAGGCTCGGAAGATTTTCCGCATCATTGAGAAAGAATTTGGAGTACCTGAGTTTGGAAAGACCAATATTACAGCTAGTAATGATCTGAACGAGGAAACATTGGATGCTTTAAACAAACAGGGTCATGAGGTTGACTCATTTGGAATTGGAACCTACGTAGTTACTTGCTATGCTCAAGCTGCTTTAGGGTGTGTTTTCAAGCTTGTTGAGATAAATAATGAGCCCAGAATTAAACTTTCTGAAGATGTTACAAAGGTCTCCATTCCTTGTAAAAAGCGGAGTTACAGACTGTACGGGAAAGAAGGTTATCCCTTGGTAGATATAATGACGGGGGAAAATGAACCACCTCCAAAGGTGGGAGAAAGAATCCTGTGTCGTCACCCTTTTAATGAATCAAAGAGAGCATACGTGGTGCCGCAGCGCGTTGAAGAACTTATGAAATGTTTCTGGCCAGGGAGTCCAGGTGGAGTAAGAGAAGATCTGCCACCATTAAAGGACATTAGGGAGCGCTGCATTACACAACTCAAGAAAATGCGTCCCGACCACATGAGGAAACTAAATCCAACGCCGTACAAGGTTAGCGTGAGTGCGAAATTGTACGAATTCATTCATTTCTTGTGGCTCAACGAAGCACCTGTTGGGGAGTTGCAGTGA

Protein Analysis

559

Amino Acids

62.07

Weight (kDa)

6.36

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 26 - 153 3.2e-39 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 112 - 156 7.8e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 432 - 543 4.9e-32 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1435
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 897
AccII CGCG 2 cut(s) 273, 1446
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 9 cut(s) 5, 183, 271, 416, 572, 981, 1042, 1300, 1439
AclWI GGATC 1 cut(s) 93
AcoI YGGCCR 2 cut(s) 733, 1472
AcsI RAATTY 2 cut(s) 1058, 1625
AcuI CTGAAG 1 cut(s) 1284
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 8 cut(s) 367, 584, 742, 971, 1068, 1316, 1595, 1622
AfeI AGCGCT 1 cut(s) 1532
AfiI CCNNNNNNNGG 6 cut(s) 82, 182, 563, 653, 1076, 1379
AgsI TTSAA 6 cut(s) 241, 344, 425, 448, 1222, 1451
AjnI CCWGG 4 cut(s) 591, 762, 1474, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 1644, 1676
AluBI AGCT 6 cut(s) 626, 681, 890, 1097, 1202, 1225
AluI AGCT 6 cut(s) 626, 681, 890, 1097, 1202, 1225
Alw26I GTCTC 2 cut(s) 818, 1285
AlwI GGATC 1 cut(s) 93
Aor13HI TCCGGA 1 cut(s) 165
Aor51HI AGCGCT 1 cut(s) 1532
AoxI GGCC 6 cut(s) 19, 49, 105, 407, 733, 1472
ApaI GGGCCC 1 cut(s) 53
ApeKI GCWGC 6 cut(s) 485, 623, 845, 1202, 1441, 1533
ApoI RAATTY 2 cut(s) 1058, 1625
Asp700I GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1466, 1629
AspLEI GCGC 2 cut(s) 1446, 1533
AspS9I GGNCC 5 cut(s) 20, 49, 50, 407, 554
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 91, 1013, 1394
AvaII GGWCC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 53
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BalI TGGCCA 2 cut(s) 735, 1474
BanI GGYRCC 1 cut(s) 1435
BanII GRGCYC 4 cut(s) 53, 550, 695, 1248
BbsI GAAGAC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 6 cut(s) 472, 635, 857, 1189, 1453, 1520
BccI CCATC 3 cut(s) 293, 554, 725
BceAI ACGGC 4 cut(s) 34, 131, 926, 1576
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciT130I CCWGG 4 cut(s) 593, 764, 1476, 1485
BciVI GTATCC 1 cut(s) 636
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 2 cut(s) 818, 1285
BfaI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
BfoI RGCGCY 1 cut(s) 1534
BfuI GTATCC 1 cut(s) 636
BglII AGATCT 1 cut(s) 1501
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 5 cut(s) 48, 593, 764, 1476, 1485
Bme18I GGWCC 1 cut(s) 554
BmgT120I GGNCC 5 cut(s) 20, 49, 50, 407, 554
BmiI GGNNCC 7 cut(s) 51, 52, 387, 555, 658, 1174, 1437
BmrFI CCNGG 5 cut(s) 48, 593, 764, 1476, 1485
BmsI GCATC 6 cut(s) 228, 473, 502, 584, 1053, 1117
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 1142, 1174
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 2 cut(s) 360, 549
BpuEI CTTGAG 3 cut(s) 548, 1182, 1532
BpuMI CCSGG 1 cut(s) 48
BsaAI YACGTR 2 cut(s) 1180, 1432
BsaI GGTCTC 2 cut(s) 818, 1285
BsaJI CCNNGG 4 cut(s) 102, 592, 1335, 1475
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 5 cut(s) 330, 360, 1471, 1501, 1658
Bsc4I CCNNNNNNNGG 6 cut(s) 82, 182, 563, 653, 1076, 1379
Bse118I RCCGGY 2 cut(s) 125, 203
Bse1I ACTGG 2 cut(s) 94, 122
Bse3DI GCAATG 2 cut(s) 228, 354
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 4 cut(s) 593, 764, 1476, 1485
BseDI CCNNGG 4 cut(s) 102, 592, 1335, 1475
BseGI GGATG 3 cut(s) 259, 304, 1132
BseLI CCNNNNNNNGG 6 cut(s) 82, 182, 563, 653, 1076, 1379
BseMI GCAATG 2 cut(s) 228, 354
BseMII CTCAG 4 cut(s) 240, 351, 563, 1062
BseNI ACTGG 2 cut(s) 94, 122
BseRI GAGGAG 2 cut(s) 336, 931
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 6 cut(s) 472, 635, 857, 1189, 1453, 1520
BseYI CCCAGC 1 cut(s) 626
Bsh1236I CGCG 2 cut(s) 273, 1446
BshFI GGCC 6 cut(s) 21, 51, 107, 409, 735, 1474
BshNI GGYRCC 1 cut(s) 1435
BsiSI CCGG 4 cut(s) 47, 126, 166, 204
BslFI GGGAC 2 cut(s) 567, 1545
BslI CCNNNNNNNGG 6 cut(s) 82, 182, 563, 653, 1076, 1379
BsmAI GTCTC 2 cut(s) 818, 1285
BsmFI GGGAC 2 cut(s) 567, 1545
BsmI GAATGC 4 cut(s) 221, 487, 848, 869
BsnI GGCC 6 cut(s) 21, 51, 107, 409, 735, 1474
Bso31I GGTCTC 2 cut(s) 818, 1285
Bsp120I GGGCCC 1 cut(s) 49
Bsp1286I GDGCHC 4 cut(s) 53, 550, 695, 1248
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 85, 1105, 1501
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 9 cut(s) 5, 183, 271, 416, 572, 981, 1042, 1300, 1439
BspANI GGCC 6 cut(s) 21, 51, 107, 409, 735, 1474
BspCNI CTCAG 4 cut(s) 241, 352, 562, 1063
BspEI TCCGGA 1 cut(s) 165
BspFNI CGCG 2 cut(s) 273, 1446
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 7 cut(s) 51, 52, 387, 555, 658, 1174, 1437
BspPI GGATC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 1435
BspTNI GGTCTC 2 cut(s) 818, 1285
BsrDI GCAATG 2 cut(s) 228, 354
BsrFI RCCGGY 2 cut(s) 125, 203
BsrI ACTGG 2 cut(s) 94, 122
BssAI RCCGGY 2 cut(s) 125, 203
BssECI CCNNGG 4 cut(s) 102, 592, 1335, 1475
BssMI GATC 3 cut(s) 85, 1105, 1501
BssT1I CCWWGG 2 cut(s) 102, 1335
Bst2UI CCWGG 4 cut(s) 593, 764, 1476, 1485
Bst4CI ACNGT 2 cut(s) 370, 1314
BstBAI YACGTR 2 cut(s) 1180, 1432
BstC8I GCNNGC 3 cut(s) 127, 131, 979
BstDEI CTNAG 5 cut(s) 249, 317, 360, 549, 1071
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 1001
BstF5I GGATG 3 cut(s) 259, 304, 1132
BstFNI CGCG 2 cut(s) 273, 1446
BstH2I RGCGCY 1 cut(s) 1534
BstHHI GCGC 2 cut(s) 1446, 1533
BstKTI GATC 3 cut(s) 88, 1108, 1504
BstMAI GTCTC 2 cut(s) 818, 1285
BstMBI GATC 3 cut(s) 85, 1105, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 482, 819, 1202
BstNI CCWGG 4 cut(s) 593, 764, 1476, 1485
BstPI GGTNACC 1 cut(s) 1001
BstSCI CCNGG 5 cut(s) 46, 591, 762, 1474, 1483
BstSLI GKGCMC 1 cut(s) 53
BstSNI TACGTA 1 cut(s) 1180
BstUI CGCG 2 cut(s) 273, 1446
BstV1I GCAGC 6 cut(s) 472, 635, 857, 1189, 1453, 1520
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 85, 1501
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 2 cut(s) 85, 1501
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 6 cut(s) 21, 51, 107, 409, 735, 1474
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 259, 304, 1132
Cac8I GCNNGC 3 cut(s) 127, 131, 979
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 2 cut(s) 1446, 1533
Cfr10I RCCGGY 2 cut(s) 125, 203
Cfr13I GGNCC 5 cut(s) 20, 49, 50, 407, 554
CseI GACGC 1 cut(s) 1547
Csp6I GTAC 8 cut(s) 366, 583, 741, 970, 1067, 1315, 1594, 1621
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
CviAII CATG 5 cut(s) 103, 605, 668, 1148, 1570
CviQI GTAC 8 cut(s) 366, 583, 741, 970, 1067, 1315, 1594, 1621
DdeI CTNAG 5 cut(s) 249, 317, 360, 549, 1071
DpnI GATC 3 cut(s) 87, 1107, 1503
DpnII GATC 3 cut(s) 85, 1105, 1501
DraI TTTAAA 1 cut(s) 1134
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 2 cut(s) 733, 1472
Eco105I TACGTA 1 cut(s) 1180
Eco130I CCWWGG 2 cut(s) 102, 1335
Eco24I GRGCYC 4 cut(s) 53, 550, 695, 1248
Eco31I GGTCTC 2 cut(s) 818, 1285
Eco47I GGWCC 1 cut(s) 554
Eco47III AGCGCT 1 cut(s) 1532
Eco57I CTGAAG 1 cut(s) 1284
Eco91I GGTNACC 1 cut(s) 1001
EcoO109I RGGNCCY 2 cut(s) 50, 554
EcoO65I GGTNACC 1 cut(s) 1001
EcoRI GAATTC 1 cut(s) 1625
EcoRII CCWGG 4 cut(s) 591, 762, 1474, 1483
EcoT14I CCWWGG 2 cut(s) 102, 1335
EcoT22I ATGCAT 3 cut(s) 221, 466, 865
EcoT38I GRGCYC 4 cut(s) 53, 550, 695, 1248
ErhI CCWWGG 2 cut(s) 102, 1335
FaeI CATG 5 cut(s) 106, 608, 671, 1151, 1573
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 2 cut(s) 567, 1545
FatI CATG 5 cut(s) 102, 604, 667, 1147, 1569
FauNDI CATATG 2 cut(s) 859, 904
FblI GTMKAC 1 cut(s) 897
FokI GGATG 3 cut(s) 266, 311, 1139
FriOI GRGCYC 4 cut(s) 53, 550, 695, 1248
FspBI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
GlaI GCGC 2 cut(s) 1445, 1532
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 1 cut(s) 1534
HaeIII GGCC 6 cut(s) 21, 51, 107, 409, 735, 1474
HapII CCGG 4 cut(s) 47, 126, 166, 204
HgaI GACGC 1 cut(s) 1547
HhaI GCGC 2 cut(s) 1446, 1533
Hin1II CATG 5 cut(s) 106, 608, 671, 1151, 1573
Hin6I GCGC 2 cut(s) 1444, 1531
HinP1I GCGC 2 cut(s) 1444, 1531
HincII GTYRAC 1 cut(s) 1156
HindII GTYRAC 1 cut(s) 1156
HindIII AAGCTT 1 cut(s) 1223
HpaII CCGG 4 cut(s) 47, 126, 166, 204
HphI GGTGA 3 cut(s) 91, 1013, 1394
Hpy166II GTNNAC 4 cut(s) 99, 839, 898, 1156
Hpy188I TCNGA 5 cut(s) 224, 771, 1032, 1110, 1264
Hpy188III TCNNGA 5 cut(s) 166, 640, 1148, 1549, 1562
Hpy8I GTNNAC 4 cut(s) 99, 839, 898, 1156
Hpy99I CGWCG 1 cut(s) 546
HpyAV CCTTC 3 cut(s) 187, 398, 1319
HpyCH4III ACNGT 2 cut(s) 370, 1314
HpyCH4IV ACGT 2 cut(s) 1179, 1431
HpyCH4V TGCA 8 cut(s) 219, 464, 488, 608, 623, 863, 1536, 1672
HpyF10VI GCNNNNNNNGC 3 cut(s) 482, 819, 1202
HpyF3I CTNAG 5 cut(s) 249, 317, 360, 549, 1071
HpySE526I ACGT 2 cut(s) 1179, 1431
Hsp92II CATG 5 cut(s) 106, 608, 671, 1151, 1573
HspAI GCGC 2 cut(s) 1444, 1531
Kpn2I TCCGGA 1 cut(s) 165
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 85, 1105, 1501
LmnI GCTCC 1 cut(s) 1528
Lsp1109I GCAGC 6 cut(s) 472, 635, 857, 1189, 1453, 1520
LweI GCATC 6 cut(s) 228, 473, 502, 584, 1053, 1117
MaeI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
MaeII ACGT 2 cut(s) 1179, 1431
MaeIII GTNAC 7 cut(s) 67, 878, 1001, 1183, 1270, 1304, 1400
MalI GATC 3 cut(s) 87, 1107, 1503
MboI GATC 3 cut(s) 85, 1105, 1501
MboII GAAGA 9 cut(s) 181, 224, 276, 279, 529, 1045, 1277, 1463, 1511
MfeI CAATTG 2 cut(s) 411, 425
MflI RGATCY 2 cut(s) 85, 1501
MhlI GDGCHC 4 cut(s) 53, 550, 695, 1248
MlsI TGGCCA 2 cut(s) 735, 1474
MluNI TGGCCA 2 cut(s) 735, 1474
MlyI GAGTC 3 cut(s) 408, 1151, 1489
MmeI TCCRAC 2 cut(s) 972, 1610
Mox20I TGGCCA 2 cut(s) 735, 1474
Mph1103I ATGCAT 3 cut(s) 221, 466, 865
MroI TCCGGA 1 cut(s) 165
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1466, 1629
MscI TGGCCA 2 cut(s) 735, 1474
MseI TTAA 8 cut(s) 456, 779, 789, 806, 1133, 1254, 1410, 1514
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 2 cut(s) 735, 1474
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 4 cut(s) 47, 126, 166, 204
MspR9I CCNGG 5 cut(s) 48, 593, 764, 1476, 1485
MunI CAATTG 2 cut(s) 411, 425
Mva1269I GAATGC 4 cut(s) 221, 487, 848, 869
MvaI CCWGG 4 cut(s) 593, 764, 1476, 1485
MvnI CGCG 2 cut(s) 273, 1446
MwoI GCNNNNNNNGC 3 cut(s) 482, 819, 1202
NaeI GCCGGC 1 cut(s) 127
NciI CCSGG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 2 cut(s) 859, 904
NdeII GATC 3 cut(s) 85, 1105, 1501
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 5 cut(s) 106, 608, 671, 1151, 1573
NlaIV GGNNCC 7 cut(s) 51, 52, 387, 555, 658, 1174, 1437
NmuCI GTSAC 3 cut(s) 67, 1001, 1400
NsiI ATGCAT 3 cut(s) 221, 466, 865
PagI TCATGA 1 cut(s) 1147
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 144
PctI GAATGC 4 cut(s) 221, 487, 848, 869
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1466, 1629
PflMI CCANNNNNTGG 1 cut(s) 653
PleI GAGTC 3 cut(s) 407, 1151, 1488
PpsI GAGTC 3 cut(s) 407, 1151, 1488
Ppu21I YACGTR 2 cut(s) 1180, 1432
PpuMI RGGWCCY 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 554
Psp6I CCWGG 4 cut(s) 591, 762, 1474, 1483
PspEI GGTNACC 1 cut(s) 1001
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 4 cut(s) 591, 762, 1474, 1483
PspN4I GGNNCC 7 cut(s) 51, 52, 387, 555, 658, 1174, 1437
PspOMI GGGCCC 1 cut(s) 49
PspPI GGNCC 5 cut(s) 20, 49, 50, 407, 554
PspPPI RGGWCCY 1 cut(s) 554
PsuI RGATCY 2 cut(s) 85, 1501
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 8 cut(s) 367, 584, 742, 971, 1068, 1316, 1595, 1622
RsaNI GTAC 8 cut(s) 366, 583, 741, 970, 1067, 1315, 1594, 1621
RseI CAYNNNNRTG 1 cut(s) 666
SaqAI TTAA 8 cut(s) 456, 779, 789, 806, 1133, 1254, 1410, 1514
Sau3AI GATC 3 cut(s) 85, 1105, 1501
Sau96I GGNCC 5 cut(s) 20, 49, 50, 407, 554
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 3 cut(s) 408, 1151, 1489
ScrFI CCNGG 5 cut(s) 48, 593, 764, 1476, 1485
SduI GDGCHC 4 cut(s) 53, 550, 695, 1248
SfaNI GCATC 6 cut(s) 228, 473, 502, 584, 1053, 1117
SinI GGWCC 1 cut(s) 554
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 3 cut(s) 527, 1197, 1547
SmoI CTYRAG 3 cut(s) 527, 1197, 1547
SnaBI TACGTA 1 cut(s) 1180
SsiI CCGC 9 cut(s) 5, 183, 271, 416, 572, 981, 1042, 1300, 1439
SspI AATATT 1 cut(s) 1090
SspMI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
StyD4I CCNGG 5 cut(s) 46, 591, 762, 1474, 1483
StyI CCWWGG 2 cut(s) 102, 1335
TaaI ACNGT 2 cut(s) 370, 1314
TaiI ACGT 2 cut(s) 1182, 1434
TaqI TCGA 6 cut(s) 14, 397, 402, 432, 515, 541
TatI WGTACW 1 cut(s) 740
TauI GCSGC 4 cut(s) 8, 186, 575, 1441
Tru1I TTAA 8 cut(s) 456, 779, 789, 806, 1133, 1254, 1410, 1514
Tru9I TTAA 8 cut(s) 456, 779, 789, 806, 1133, 1254, 1410, 1514
TseFI GTSAC 3 cut(s) 67, 1001, 1400
TseI GCWGC 6 cut(s) 485, 623, 845, 1202, 1441, 1533
Tsp45I GTSAC 3 cut(s) 67, 1001, 1400
TspDTI ATGAA 8 cut(s) 217, 270, 593, 1377, 1428, 1475, 1618, 1622
TspGWI ACGGA 1 cut(s) 98
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 554
XapI RAATTY 2 cut(s) 1058, 1625
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 6 cut(s) 176, 635, 661, 1037, 1466, 1629
XspI CTAG 5 cut(s) 842, 876, 887, 1007, 1098
ZrmI AGTACT 1 cut(s) 742
Zsp2I ATGCAT 3 cut(s) 221, 466, 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.