Rh2DG189000

Nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005674
Physical Location & Seq
Reverse (-)
16968312 .. 16974921
6610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG189000.1

Sequence Viewer

Length: 1665 bp
ATGGCAGCCATATCCAACGGCCAGGTCCTCGCCGGCCCCACCAATCCAATGGTCACGCCTCTCCTCACCGATCTCTACCAGTTCACCATGGCCTATGCTTACTGGAAAGCCGGCAAGCACAACGAACGAGCCGTGTTTGATTTGTATTTCCGCAAGAATCCATTTGGCGGCGAATACACAGTGTTTGCTGGATTAGAGGAATGCATCAAATTCATTGCTAATTTCAAATTGGCCGACGACGAAATCGATTTCGTCCGCCAATCCTTGTCTTCTTCGTGTGAGGAAGGATTCTATAAGTATCTGAGTGGAGTTGACTGTTCCGAAGTTGAAGTCTATGCTATCCAAGAAGGATCAGTCGTTTTTCCAAAGGTTCCTCTCGTTAGAGTTGAAGGACCAATCGCTGTGGTTCAGTTACTCGAAACTCCATTCTTGAATCTCATTAATTATGCGTCATTAGTTGCTACAAATGCCGCAAGGCATCGTTTCGTTGCTGGAGAATCTAAAATGCTACTTGAGTTTGGGCTTCGACGAGCTCAGGGACCTGATGGTGGAATAGGGGCATCTAGGTACTGCTACATGGGAGGATTTCATGCAACAAGTAATGTTGCGGCTGGAAAGATATTTGGGATCCCGCTTCGTGGAACACATTCGCATGCTTTTGTCAGCTCATTCATGAGCCCAGATGAGATTATAGACAAATCACTTCATAGCAGTGATAAGCGCAGTACTTGTGAGGATTTTGTTAGCCTGGTTCAGACTTGGTTGAGCAAAATTCAGTGGTCAAATTCATTAAATGGTACTTTTGGTGAGACCAATCATAGTGAGCTTGCAGCATTCACATCGTATGCCCTGGCATTCCCTGATAACTTTCTAGCTCTTGTAGACACATATGATGTTATGAGGAGTGGAATTCCCAACTTTTGTGCAGTTGCTTTAGCACTCAATGATCTAGGGTACAAAGCAGCTGGCATTAGGTTGGATTCAGGTGATCTAGCATATCTCTCCTCTGAGGCTCGGAAGATTTTTCGCATCATTGAGAAAGAATTTGGAGTACCTGGATTTGGAAAGACGGTTGTCACAGCTAGTAATGATCTAAATGAGGAAACTTTGGATGCTTTAAACAAACAGGGCCATGAGGTTGATGCATTTGGAATTGGGACCCATGTAGTTACTTGTTATGCTCAAGCTGCTCTGGGTTGTGTTTTCAAGCTTGTTGAGATAAATAATCAGCCTCGCATCAAACTTTCTGAAGATGTCACAAAGGTCTCCATCCCTTGTAAAAAACGATCTTACAGATTGTTTGGGAAAGAAGGTTATCCCCTGGTAGACATAATGACCGGAGAAAATGAACCTCCTCCAAAGGTTGGAGAACGGATCCTGTGTCGTCACCCCTTTAATGAATCGAAGAGAGCATATGTAGTGCCGCAGCGTGTTGAGGAACTTATGAAATGCTTTTGGCATGGCCCAGGTATGGGTTCCGGATTTGTCAATCTTCTATCTGAAATCACTGCCTGCTCATTCTTGTTATGGCTATCAGTTTTATCATTTAACCCCACAGTTTTCTCAGATATAGTTATTATGTTACTTTCTAAGACAACAGAAAGCCATGCGCACCTGAAGTTGATGCTTGATATACTTATTTGTTTACCATTGTGCTTCTTGTAA

Protein Analysis

554

Amino Acids

61.16

Weight (kDa)

5.86

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 21 - 148 1.8e-38 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 107 - 151 3e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 427 - 487 5.4e-15 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1611
AccB7I CCANNNNNTGG 1 cut(s) 1364
AccI GTMKAC 2 cut(s) 882, 1326
AccIII TCCGGA 1 cut(s) 1478
AciI CCGC 7 cut(s) 151, 168, 256, 471, 608, 632, 1424
AclWI GGATC 5 cut(s) 358, 622, 635, 1369, 1382
AcoI YGGCCR 2 cut(s) 19, 231
AcsI RAATTY 5 cut(s) 209, 771, 784, 909, 1043
AcuI CTGAAG 2 cut(s) 1269, 1637
AfaI GTAC 5 cut(s) 569, 727, 799, 956, 1053
AfiI CCNNNNNNNGG 7 cut(s) 167, 548, 638, 1061, 1364, 1471, 1472
AgsI TTSAA 5 cut(s) 226, 329, 389, 433, 1207
AjnI CCWGG 6 cut(s) 21, 747, 849, 1054, 1320, 1465
AluBI AGCT 8 cut(s) 533, 666, 826, 875, 965, 1082, 1187, 1210
AluI AGCT 8 cut(s) 533, 666, 826, 875, 965, 1082, 1187, 1210
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 2 cut(s) 803, 1270
AlwI GGATC 5 cut(s) 358, 622, 635, 1369, 1382
Aor13HI TCCGGA 1 cut(s) 1478
AoxI GGCC 6 cut(s) 19, 34, 90, 231, 1129, 1462
ApeKI GCWGC 5 cut(s) 5, 830, 962, 1187, 1426
ApoI RAATTY 5 cut(s) 209, 771, 784, 909, 1043
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AseI ATTAAT 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 646
AspLEI GCGC 2 cut(s) 723, 1612
AspS9I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
AsuHPI GGTGA 5 cut(s) 58, 76, 818, 998, 1379
AvaII GGWCC 4 cut(s) 25, 392, 539, 1158
BamHI GGATCC 2 cut(s) 627, 1374
BanII GRGCYC 2 cut(s) 535, 680
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 5 cut(s) 17, 842, 974, 1174, 1438
BccI CCATC 2 cut(s) 539, 1277
BceAI ACGGC 2 cut(s) 34, 116
BcgI CGANNNNNNTGC 2 cut(s) 822, 856
BciT130I CCWGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
BcoDI GTCTC 2 cut(s) 803, 1270
BfaI CTAG 5 cut(s) 564, 872, 950, 992, 1083
BisI GCNGC 9 cut(s) 6, 169, 471, 609, 831, 963, 1188, 1424, 1427
BlsI GCNGC 9 cut(s) 7, 170, 472, 610, 832, 964, 1189, 1425, 1428
BmcAI AGTACT 1 cut(s) 727
Bme1390I CCNGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
Bme18I GGWCC 4 cut(s) 25, 392, 539, 1158
BmgT120I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
BmiI GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1477
BmrFI CCNGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
BmsI GCATC 8 cut(s) 213, 487, 569, 1038, 1102, 1132, 1245, 1614
BoxI GACNNNNGTC 1 cut(s) 1073
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 2 cut(s) 533, 1167
Bsa29I ATCGAT 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 803, 1270
BsaJI CCNNGG 4 cut(s) 87, 849, 1320, 1465
BsaWI WCCGGW 2 cut(s) 1337, 1478
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 548, 638, 1061, 1364, 1471, 1472
Bse118I RCCGGY 2 cut(s) 32, 110
Bse1I ACTGG 2 cut(s) 79, 107
Bse3DI GCAATG 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 1478
BseBI CCWGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 4 cut(s) 87, 849, 1320, 1465
BseGI GGATG 2 cut(s) 1117, 1269
BseLI CCNNNNNNNGG 7 cut(s) 167, 548, 638, 1061, 1364, 1471, 1472
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 4 cut(s) 293, 548, 999, 1578
BseNI ACTGG 2 cut(s) 79, 107
BseRI GAGGAG 4 cut(s) 53, 916, 994, 1344
BseXI GCAGC 5 cut(s) 17, 842, 974, 1174, 1438
BsgI GTGCAG 1 cut(s) 945
BshFI GGCC 6 cut(s) 21, 36, 92, 233, 1131, 1464
BshVI ATCGAT 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 535
BsiSI CCGG 4 cut(s) 33, 111, 1338, 1479
BslFI GGGAC 2 cut(s) 552, 1171
BslI CCNNNNNNNGG 7 cut(s) 167, 548, 638, 1061, 1364, 1471, 1472
BsmAI GTCTC 2 cut(s) 803, 1270
BsmFI GGGAC 2 cut(s) 552, 1171
BsmI GAATGC 3 cut(s) 206, 833, 854
BsnI GGCC 6 cut(s) 21, 36, 92, 233, 1131, 1464
Bso31I GGTCTC 2 cut(s) 803, 1270
Bsp1286I GDGCHC 2 cut(s) 535, 680
Bsp13I TCCGGA 1 cut(s) 1478
Bsp143I GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 7 cut(s) 151, 168, 256, 471, 608, 632, 1424
BspANI GGCC 6 cut(s) 21, 36, 92, 233, 1131, 1464
BspCNI CTCAG 4 cut(s) 294, 547, 1000, 1577
BspDI ATCGAT 1 cut(s) 246
BspEI TCCGGA 1 cut(s) 1478
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1477
BspPI GGATC 5 cut(s) 358, 622, 635, 1369, 1382
BspTNI GGTCTC 2 cut(s) 803, 1270
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 2 cut(s) 32, 110
BsrI ACTGG 2 cut(s) 79, 107
BssAI RCCGGY 2 cut(s) 32, 110
BssECI CCNNGG 4 cut(s) 87, 849, 1320, 1465
BssMI GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
Bst4CI ACNGT 4 cut(s) 181, 317, 1072, 1558
Bst6I CTCTTC 1 cut(s) 1400
BstC8I GCNNGC 7 cut(s) 34, 112, 116, 654, 828, 967, 1513
BstDEI CTNAG 5 cut(s) 302, 534, 1008, 1564, 1590
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 1117, 1269
BstHHI GCGC 2 cut(s) 723, 1612
BstKTI GATC 8 cut(s) 73, 353, 630, 949, 991, 1093, 1289, 1377
BstMAI GTCTC 2 cut(s) 803, 1270
BstMBI GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
BstMWI GCNNNNNNNGC 2 cut(s) 467, 1187
BstNI CCWGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
BstNSI RCATGY 1 cut(s) 656
BstPAI GACNNNNGTC 1 cut(s) 1073
BstSCI CCNGG 6 cut(s) 21, 747, 849, 1054, 1320, 1465
BstV1I GCAGC 5 cut(s) 17, 842, 974, 1174, 1438
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 2 cut(s) 627, 1374
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 2 cut(s) 627, 1374
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 6 cut(s) 21, 36, 92, 233, 1131, 1464
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 1117, 1269
BtsI GCAGTG 2 cut(s) 718, 1506
BtsIMutI CAGTG 4 cut(s) 186, 718, 782, 1506
Cac8I GCNNGC 7 cut(s) 34, 112, 116, 654, 828, 967, 1513
CciI TCATGA 1 cut(s) 672
CfoI GCGC 2 cut(s) 723, 1612
Cfr10I RCCGGY 2 cut(s) 32, 110
Cfr13I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 1 cut(s) 438
Csp6I GTAC 5 cut(s) 568, 726, 798, 955, 1052
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 9 cut(s) 88, 577, 590, 653, 673, 1133, 1163, 1460, 1607
CviQI GTAC 5 cut(s) 568, 726, 798, 955, 1052
DdeI CTNAG 5 cut(s) 302, 534, 1008, 1564, 1590
DpnI GATC 8 cut(s) 72, 352, 629, 948, 990, 1092, 1288, 1376
DpnII GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
DraI TTTAAA 1 cut(s) 1119
EaeI YGGCCR 2 cut(s) 19, 231
Eam1104I CTCTTC 1 cut(s) 1400
EarI CTCTTC 1 cut(s) 1400
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 533
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 2 cut(s) 535, 680
Eco31I GGTCTC 2 cut(s) 803, 1270
Eco47I GGWCC 4 cut(s) 25, 392, 539, 1158
Eco53kI GAGCTC 1 cut(s) 533
Eco57I CTGAAG 2 cut(s) 1269, 1637
EcoICRI GAGCTC 1 cut(s) 533
EcoO109I RGGNCCY 3 cut(s) 25, 539, 1158
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 6 cut(s) 21, 747, 849, 1054, 1320, 1465
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 2 cut(s) 206, 1147
EcoT38I GRGCYC 2 cut(s) 535, 680
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 9 cut(s) 91, 580, 593, 656, 676, 1136, 1166, 1463, 1610
FaqI GGGAC 2 cut(s) 552, 1171
FatI CATG 9 cut(s) 87, 576, 589, 652, 672, 1132, 1162, 1459, 1606
FauI CCCGC 1 cut(s) 639
FauNDI CATATG 2 cut(s) 889, 1414
FblI GTMKAC 2 cut(s) 882, 1326
Fnu4HI GCNGC 9 cut(s) 6, 169, 471, 609, 831, 963, 1188, 1424, 1427
FokI GGATG 2 cut(s) 1124, 1256
FriOI GRGCYC 2 cut(s) 535, 680
Fsp4HI GCNGC 9 cut(s) 6, 169, 471, 609, 831, 963, 1188, 1424, 1427
FspAI RTGCGCAY 1 cut(s) 1611
FspBI CTAG 5 cut(s) 564, 872, 950, 992, 1083
FspI TGCGCA 1 cut(s) 1611
GlaI GCGC 2 cut(s) 722, 1611
GluI GCNGC 9 cut(s) 6, 169, 471, 609, 831, 963, 1188, 1424, 1427
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 6 cut(s) 21, 36, 92, 233, 1131, 1464
HapII CCGG 4 cut(s) 33, 111, 1338, 1479
HgaI GACGC 1 cut(s) 438
HhaI GCGC 2 cut(s) 723, 1612
Hin1II CATG 9 cut(s) 91, 580, 593, 656, 676, 1136, 1166, 1463, 1610
Hin6I GCGC 2 cut(s) 721, 1610
HinP1I GCGC 2 cut(s) 721, 1610
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 6 cut(s) 157, 288, 433, 497, 980, 1400
HpaII CCGG 4 cut(s) 33, 111, 1338, 1479
HphI GGTGA 5 cut(s) 58, 76, 818, 998, 1379
Hpy166II GTNNAC 5 cut(s) 84, 313, 883, 1327, 1646
Hpy188I TCNGA 8 cut(s) 303, 322, 756, 1009, 1017, 1249, 1501, 1567
Hpy188III TCNNGA 3 cut(s) 430, 673, 1479
Hpy8I GTNNAC 5 cut(s) 84, 313, 883, 1327, 1646
Hpy99I CGWCG 3 cut(s) 239, 242, 531
HpyAV CCTTC 4 cut(s) 278, 341, 383, 1304
HpyCH4III ACNGT 4 cut(s) 181, 317, 1072, 1558
HpyCH4V TGCA 5 cut(s) 204, 593, 830, 926, 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 467, 1187
HpyF3I CTNAG 5 cut(s) 302, 534, 1008, 1564, 1590
Hsp92II CATG 9 cut(s) 91, 580, 593, 656, 676, 1136, 1166, 1463, 1610
HspAI GCGC 2 cut(s) 721, 1610
KflI GGGWCCC 1 cut(s) 1158
Kpn2I TCCGGA 1 cut(s) 1478
KroI GCCGGC 2 cut(s) 32, 110
KroNI GCCGGC 2 cut(s) 34, 112
Kzo9I GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
Lsp1109I GCAGC 5 cut(s) 17, 842, 974, 1174, 1438
LweI GCATC 8 cut(s) 213, 487, 569, 1038, 1102, 1132, 1245, 1614
MaeI CTAG 5 cut(s) 564, 872, 950, 992, 1083
MaeIII GTNAC 7 cut(s) 52, 411, 1075, 1168, 1255, 1385, 1581
MalI GATC 8 cut(s) 72, 352, 629, 948, 990, 1092, 1288, 1376
MboI GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
MboII GAAGA 6 cut(s) 261, 264, 1030, 1262, 1417, 1484
MflI RGATCY 2 cut(s) 627, 1374
MhlI GDGCHC 2 cut(s) 535, 680
MluCI AATT 9 cut(s) 209, 220, 227, 442, 771, 784, 909, 1043, 1152
MmeI TCCRAC 3 cut(s) 39, 957, 1345
Mph1103I ATGCAT 2 cut(s) 206, 1147
MroI TCCGGA 1 cut(s) 1478
MroNI GCCGGC 2 cut(s) 32, 110
MroXI GAANNNNTTC 1 cut(s) 646
MseI TTAA 5 cut(s) 441, 791, 1118, 1395, 1548
MslI CAYNNNNRTG 2 cut(s) 651, 711
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 4 cut(s) 33, 111, 1338, 1479
MspR9I CCNGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
Mva1269I GAATGC 3 cut(s) 206, 833, 854
MvaI CCWGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
MwoI GCNNNNNNNGC 2 cut(s) 467, 1187
NaeI GCCGGC 2 cut(s) 34, 112
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 2 cut(s) 889, 1414
NdeII GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
NgoMIV GCCGGC 2 cut(s) 32, 110
NlaIII CATG 9 cut(s) 91, 580, 593, 656, 676, 1136, 1166, 1463, 1610
NlaIV GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1477
NmuCI GTSAC 4 cut(s) 52, 1075, 1255, 1385
NsbI TGCGCA 1 cut(s) 1611
NsiI ATGCAT 2 cut(s) 206, 1147
NspI RCATGY 1 cut(s) 656
PaeI GCATGC 1 cut(s) 656
PagI TCATGA 1 cut(s) 672
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 243
PctI GAATGC 3 cut(s) 206, 833, 854
PdiI GCCGGC 2 cut(s) 34, 112
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 6 cut(s) 157, 288, 433, 497, 980, 1400
PflMI CCANNNNNTGG 1 cut(s) 1364
PkrI GCNGC 9 cut(s) 7, 170, 472, 610, 832, 964, 1189, 1425, 1428
PpuMI RGGWCCY 3 cut(s) 25, 539, 1158
PshAI GACNNNNGTC 1 cut(s) 1073
PshBI ATTAAT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 535
Psp5II RGGWCCY 3 cut(s) 25, 539, 1158
Psp6I CCWGG 6 cut(s) 21, 747, 849, 1054, 1320, 1465
PspGI CCWGG 6 cut(s) 21, 747, 849, 1054, 1320, 1465
PspN4I GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1477
PspPI GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
PspPPI RGGWCCY 3 cut(s) 25, 539, 1158
PsuI RGATCY 2 cut(s) 627, 1374
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 5 cut(s) 569, 727, 799, 956, 1053
RsaNI GTAC 5 cut(s) 568, 726, 798, 955, 1052
RseI CAYNNNNRTG 2 cut(s) 651, 711
SacI GAGCTC 1 cut(s) 535
SaqAI TTAA 5 cut(s) 441, 791, 1118, 1395, 1548
SatI GCNGC 9 cut(s) 6, 169, 471, 609, 831, 963, 1188, 1424, 1427
Sau3AI GATC 8 cut(s) 70, 350, 627, 946, 988, 1090, 1286, 1374
Sau96I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
ScaI AGTACT 1 cut(s) 727
ScrFI CCNGG 6 cut(s) 23, 749, 851, 1056, 1322, 1467
SduI GDGCHC 2 cut(s) 535, 680
SfaNI GCATC 8 cut(s) 213, 487, 569, 1038, 1102, 1132, 1245, 1614
SinI GGWCC 4 cut(s) 25, 392, 539, 1158
SmiMI CAYNNNNRTG 2 cut(s) 651, 711
SmlI CTYRAG 2 cut(s) 512, 1182
SmoI CTYRAG 2 cut(s) 512, 1182
SphI GCATGC 1 cut(s) 656
Sse9I AATT 9 cut(s) 209, 220, 227, 442, 771, 784, 909, 1043, 1152
SsiI CCGC 7 cut(s) 151, 168, 256, 471, 608, 632, 1424
SspMI CTAG 5 cut(s) 564, 872, 950, 992, 1083
SstI GAGCTC 1 cut(s) 535
StyD4I CCNGG 6 cut(s) 21, 747, 849, 1054, 1320, 1465
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 181, 317, 1072, 1558
TaqI TCGA 4 cut(s) 246, 417, 526, 1403
TasI AATT 9 cut(s) 209, 220, 227, 442, 771, 784, 909, 1043, 1152
TatI WGTACW 1 cut(s) 725
TauI GCSGC 4 cut(s) 171, 473, 611, 1426
TfiI GAWTC 6 cut(s) 157, 288, 433, 497, 980, 1400
Tru1I TTAA 5 cut(s) 441, 791, 1118, 1395, 1548
Tru9I TTAA 5 cut(s) 441, 791, 1118, 1395, 1548
TscAI CASTG 4 cut(s) 186, 718, 782, 1513
TseFI GTSAC 4 cut(s) 52, 1075, 1255, 1385
TseI GCWGC 5 cut(s) 5, 830, 962, 1187, 1426
Tsp45I GTSAC 4 cut(s) 52, 1075, 1255, 1385
TspDTI ATGAA 8 cut(s) 202, 578, 661, 695, 777, 1362, 1413, 1460
TspGWI ACGGA 1 cut(s) 1387
TspRI CASTG 4 cut(s) 186, 718, 782, 1513
Van91I CCANNNNNTGG 1 cut(s) 1364
VpaK11BI GGWCC 4 cut(s) 25, 392, 539, 1158
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 5 cut(s) 209, 771, 784, 909, 1043
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 882, 1326
XmnI GAANNNNTTC 1 cut(s) 646
XspI CTAG 5 cut(s) 564, 872, 950, 992, 1083
ZrmI AGTACT 1 cut(s) 727
Zsp2I ATGCAT 2 cut(s) 206, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.