FvH4_1g16212

Nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005674
Physical Location & Seq
Reverse (-)
9287729 .. 9293452
5724 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16212.t2

Sequence Viewer

Length: 1659 bp
ATGGCGGCCAAATCCAACGGCCAGGCCCTCGCCGGCCCCACCAATCCAATGGTGACGCCTCTCCTCACCGATCTCTACCAGTTCACCATGGCCTACGCTTACTGGAAAGCCGGCAAGCACAACGAACGCGCCGTGTTTGATTTGTATTTCCGGAAGAATCCGTTCGGCGGCGAGTACACTGTGTTCGCCGGTCTAGAGGAATGCATCAAATTCATTGCTAATTTCAAATTGGCCGAGGACGAAATCGATTTCGTCCGCCAATCCTTGTCTTCTTCGTGTGAGGAAGGATTCTTTGACTATCTGAGAGGAGTTGACTGTGCCGATGTCGAAGTGTATGCTATCCAGGAGGGATCAGTTGTTTTTCCGAAGGTTCCTTTGGTTAGAGTTGAAGGACCTATCGCTGTGGTTCAGTTGCTCGAAACACCGTTCTTGAATCTCATTAACTACGCGTCATTAGTTTCTACAAATGCCGCGAGGCATCGTTTCGTTGCTGGAGAATCTAAAATGCTACTCGAGTTTGGGCTTCGTCGAGCTCAGGGACCTGATGGTGGAATAGCGGCATCTAGGTACTGCTATATGGGAGGATTTCATGCAACAAGTAATGTTGCCGCTGGAAAGATATTTGGGATCCCGCTTCGTGGAACACATTCACATGCTTTTGTCAGCTCATTCATGAGCCCAGACGAGATTATAGATAAAACACTGCATAGCAGTGATAAAAGCAGTACTTGTGAGGATTTTGTTAGCCTGGTTCAGACTTGGTTGAGCAAAATTCAGTGGTCAAAGTCATTAAATGGTACTTTTGGTGAGACCAATCAAAGTGAGCTTGCAGCTTTCACATCTTATGCCCTGGCATTCCCTGATAACTTTCTAGCTCTTGTAGACACATATGATGTTATGAGGAGCGGAATCCCCAACTTCTGTGCAGTTGCTTTAGCATTAAATGATTTAGGGTACAAAGCAGCTGGTATTAGGTTGGATTCAGGTGATCTGGCATATCTCTCCTCTGAGGCTCGTAAGATTTTCAACATCATTGAGAAAGAATTTGGAGTACCTGGATTTGGAAAGACACTTATCACAGCTAGTAATGATCTAAATGAAGAAACTTTGGATGCTTTAAATAAACAGGGCCATGAGGTTGATGCATTTGGAATTGGGACCCATGTAGTTACTTGCTATGCTCAAGCTGCTCTTGGTTGTGTTTTCAAGCTTGTTGAGATAAATAATCAACCTCGCATCAAGCTTTCTGAAGACGTTACAAAGGTCTCTATCCCTTGTAAAAAACGATCTTACAGATTGTTTGGGAAAGAAGGTTATCCCCTGGTAGACATAATGACTGGAGAAAATGAACCACCTCCAAAGGTTGGAGAACGGATCCTGTGTCGTCACCCCTTTAATGAATCAAAGAGAGCATATGTAGTACCACAGCGTGTTGAGGAACTTATGAAATGCTTTTGGCATGGCCCAGATGGAGTGAGAGAAGATTTGCCACCATTGAAGGACATACGGGAGCGCTGCATCAGCCAACTCAAGAAAATGCGTCCTGACCACATGAGGAGACTAAACCCCACTCCGTATAAGGTTAGTGTAAGTGCGAAATTGTACGACTTCATTCACTTCCTATGGCTCAACGAGGCACCTGTCGGGGAATTGCAGTGA

Protein Analysis

553

Amino Acids

61.44

Weight (kDa)

6.11

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 21 - 148 5.5e-39 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 107 - 151 3.6e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 427 - 521 1.6e-15 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1636
AccB7I CCANNNNNTGG 1 cut(s) 1364
AccBSI CCGCTC 1 cut(s) 906
AccI GTMKAC 2 cut(s) 882, 1326
AccII CGCG 3 cut(s) 129, 449, 473
AccIII TCCGGA 1 cut(s) 150
AciI CCGC 8 cut(s) 5, 168, 256, 471, 557, 609, 632, 906
AclWI GGATC 5 cut(s) 358, 622, 635, 1369, 1382
AcoI YGGCCR 3 cut(s) 6, 19, 231
AcsI RAATTY 3 cut(s) 209, 771, 1043
AcuI CTGAAG 1 cut(s) 1269
AcyI GRCGYC 1 cut(s) 56
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 8 cut(s) 176, 569, 727, 799, 956, 1053, 1422, 1604
AfeI AGCGCT 1 cut(s) 1514
AfiI CCNNNNNNNGG 5 cut(s) 167, 548, 638, 1061, 1364
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 6 cut(s) 226, 389, 433, 1027, 1207, 1498
AjnI CCWGG 6 cut(s) 21, 342, 747, 849, 1054, 1320
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 3 cut(s) 803, 1270, 1552
AlwI GGATC 5 cut(s) 358, 622, 635, 1369, 1382
Ama87I CYCGRG 1 cut(s) 512
Aor13HI TCCGGA 1 cut(s) 150
Aor51HI AGCGCT 1 cut(s) 1514
AoxI GGCC 8 cut(s) 6, 19, 24, 34, 90, 231, 1129, 1462
ApeKI GCWGC 4 cut(s) 830, 962, 1187, 1515
ApoI RAATTY 3 cut(s) 209, 771, 1043
Asp700I GAANNNNTTC 2 cut(s) 161, 646
AspLEI GCGC 2 cut(s) 131, 1515
AspS9I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
AsuHPI GGTGA 6 cut(s) 58, 64, 76, 818, 998, 1379
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 3 cut(s) 392, 539, 1158
BamHI GGATCC 2 cut(s) 627, 1374
BanI GGYRCC 1 cut(s) 1636
BanII GRGCYC 2 cut(s) 535, 680
BbsI GAAGAC 2 cut(s) 261, 1257
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 4 cut(s) 842, 974, 1174, 1502
BccI CCATC 2 cut(s) 539, 1463
BceAI ACGGC 2 cut(s) 34, 116
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
BcoDI GTCTC 3 cut(s) 803, 1270, 1552
BfaI CTAG 4 cut(s) 194, 564, 872, 1083
BfoI RGCGCY 1 cut(s) 1516
BisI GCNGC 9 cut(s) 6, 169, 471, 558, 609, 831, 963, 1188, 1516
BlsI GCNGC 9 cut(s) 7, 170, 472, 559, 610, 832, 964, 1189, 1517
BmcAI AGTACT 1 cut(s) 727
Bme1390I CCNGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
Bme18I GGWCC 3 cut(s) 392, 539, 1158
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
BmiI GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1638
BmrFI CCNGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
BmsI GCATC 7 cut(s) 213, 487, 569, 1102, 1132, 1245, 1527
BpiI GAAGAC 2 cut(s) 261, 1257
BpmI CTGGAG 2 cut(s) 513, 1359
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 2 cut(s) 1167, 1514
Bsa29I ATCGAT 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 2 cut(s) 803, 1270
BsaJI CCNNGG 4 cut(s) 87, 234, 849, 1320
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 548, 638, 1061, 1364
Bse118I RCCGGY 3 cut(s) 32, 110, 188
Bse1I ACTGG 3 cut(s) 79, 107, 1342
Bse3DI GCAATG 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 150
BseBI CCWGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 4 cut(s) 87, 234, 849, 1320
BseGI GGATG 1 cut(s) 1117
BseLI CCNNNNNNNGG 5 cut(s) 167, 548, 638, 1061, 1364
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 3 cut(s) 293, 548, 999
BseNI ACTGG 3 cut(s) 79, 107, 1342
BseRI GAGGAG 5 cut(s) 53, 321, 916, 994, 1570
BseXI GCAGC 4 cut(s) 842, 974, 1174, 1502
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 3 cut(s) 129, 449, 473
BshFI GGCC 8 cut(s) 8, 21, 26, 36, 92, 233, 1131, 1464
BshNI GGYRCC 1 cut(s) 1636
BshVI ATCGAT 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 535
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 33, 111, 151, 189
BslFI GGGAC 2 cut(s) 552, 1171
BslI CCNNNNNNNGG 5 cut(s) 167, 548, 638, 1061, 1364
BsmAI GTCTC 3 cut(s) 803, 1270, 1552
BsmFI GGGAC 2 cut(s) 552, 1171
BsmI GAATGC 2 cut(s) 206, 854
BsnI GGCC 8 cut(s) 8, 21, 26, 36, 92, 233, 1131, 1464
Bso31I GGTCTC 2 cut(s) 803, 1270
BsoBI CYCGRG 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 535, 680
Bsp13I TCCGGA 1 cut(s) 150
Bsp143I GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 8 cut(s) 5, 168, 256, 471, 557, 609, 632, 906
BspANI GGCC 8 cut(s) 8, 21, 26, 36, 92, 233, 1131, 1464
BspCNI CTCAG 3 cut(s) 294, 547, 1000
BspDI ATCGAT 1 cut(s) 246
BspEI TCCGGA 1 cut(s) 150
BspFNI CGCG 3 cut(s) 129, 449, 473
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1638
BspPI GGATC 5 cut(s) 358, 622, 635, 1369, 1382
BspT107I GGYRCC 1 cut(s) 1636
BspTNI GGTCTC 2 cut(s) 803, 1270
BsrBI CCGCTC 1 cut(s) 906
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 3 cut(s) 32, 110, 188
BsrI ACTGG 3 cut(s) 79, 107, 1342
BssAI RCCGGY 3 cut(s) 32, 110, 188
BssECI CCNNGG 4 cut(s) 87, 234, 849, 1320
BssMI GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
BssNI GRCGYC 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
Bst4CI ACNGT 3 cut(s) 181, 317, 426
BstACI GRCGYC 1 cut(s) 56
BstC8I GCNNGC 4 cut(s) 34, 112, 116, 828
BstDEI CTNAG 3 cut(s) 302, 534, 1008
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 1117
BstFNI CGCG 3 cut(s) 129, 449, 473
BstH2I RGCGCY 1 cut(s) 1516
BstHHI GCGC 2 cut(s) 131, 1515
BstKTI GATC 7 cut(s) 73, 353, 630, 991, 1093, 1289, 1377
BstMAI GTCTC 3 cut(s) 803, 1270, 1552
BstMBI GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
BstMWI GCNNNNNNNGC 2 cut(s) 1187, 1521
BstNI CCWGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 21, 342, 747, 849, 1054, 1320
BstUI CGCG 3 cut(s) 129, 449, 473
BstV1I GCAGC 4 cut(s) 842, 974, 1174, 1502
BstV2I GAAGAC 2 cut(s) 261, 1257
BstX2I RGATCY 2 cut(s) 627, 1374
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 2 cut(s) 627, 1374
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 8 cut(s) 8, 21, 26, 36, 92, 233, 1131, 1464
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 1117
BtsI GCAGTG 2 cut(s) 701, 718
BtsIMutI CAGTG 4 cut(s) 177, 701, 718, 782
Cac8I GCNNGC 4 cut(s) 34, 112, 116, 828
CciI TCATGA 1 cut(s) 672
CfoI GCGC 2 cut(s) 131, 1515
Cfr10I RCCGGY 3 cut(s) 32, 110, 188
Cfr13I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 3 cut(s) 64, 438, 1529
Csp6I GTAC 8 cut(s) 175, 568, 726, 798, 955, 1052, 1421, 1603
CviAII CATG 8 cut(s) 88, 590, 653, 673, 1133, 1163, 1460, 1552
CviQI GTAC 8 cut(s) 175, 568, 726, 798, 955, 1052, 1421, 1603
DdeI CTNAG 3 cut(s) 302, 534, 1008
DpnI GATC 7 cut(s) 72, 352, 629, 990, 1092, 1288, 1376
DpnII GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
DraI TTTAAA 1 cut(s) 1119
DraIII CACNNNGTG 1 cut(s) 1430
EaeI YGGCCR 3 cut(s) 6, 19, 231
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 533
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 2 cut(s) 535, 680
Eco31I GGTCTC 2 cut(s) 803, 1270
Eco47I GGWCC 3 cut(s) 392, 539, 1158
Eco47III AGCGCT 1 cut(s) 1514
Eco53kI GAGCTC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 1269
Eco88I CYCGRG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 533
EcoO109I RGGNCCY 4 cut(s) 25, 392, 539, 1158
EcoRII CCWGG 6 cut(s) 21, 342, 747, 849, 1054, 1320
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 2 cut(s) 206, 1147
EcoT38I GRGCYC 2 cut(s) 535, 680
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 8 cut(s) 91, 593, 656, 676, 1136, 1166, 1463, 1555
FalI AAGNNNNNCTT 4 cut(s) 712, 744, 1176, 1208
FaqI GGGAC 2 cut(s) 552, 1171
FatI CATG 8 cut(s) 87, 589, 652, 672, 1132, 1162, 1459, 1551
FauI CCCGC 1 cut(s) 639
FauNDI CATATG 2 cut(s) 889, 1414
FblI GTMKAC 2 cut(s) 882, 1326
Fnu4HI GCNGC 9 cut(s) 6, 169, 471, 558, 609, 831, 963, 1188, 1516
FokI GGATG 1 cut(s) 1124
FriOI GRGCYC 2 cut(s) 535, 680
Fsp4HI GCNGC 9 cut(s) 6, 169, 471, 558, 609, 831, 963, 1188, 1516
FspBI CTAG 4 cut(s) 194, 564, 872, 1083
GlaI GCGC 2 cut(s) 130, 1514
GluI GCNGC 9 cut(s) 6, 169, 471, 558, 609, 831, 963, 1188, 1516
GsuI CTGGAG 2 cut(s) 513, 1359
HaeII RGCGCY 1 cut(s) 1516
HaeIII GGCC 8 cut(s) 8, 21, 26, 36, 92, 233, 1131, 1464
HapII CCGG 4 cut(s) 33, 111, 151, 189
HgaI GACGC 3 cut(s) 64, 438, 1529
HhaI GCGC 2 cut(s) 131, 1515
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 8 cut(s) 91, 593, 656, 676, 1136, 1166, 1463, 1555
Hin6I GCGC 2 cut(s) 129, 1513
HinP1I GCGC 2 cut(s) 129, 1513
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 2 cut(s) 1208, 1241
HinfI GANTC 7 cut(s) 157, 288, 433, 497, 909, 980, 1400
HpaII CCGG 4 cut(s) 33, 111, 151, 189
HphI GGTGA 6 cut(s) 58, 64, 76, 818, 998, 1379
Hpy166II GTNNAC 5 cut(s) 84, 177, 313, 883, 1327
Hpy188I TCNGA 5 cut(s) 303, 366, 756, 1009, 1249
Hpy188III TCNNGA 6 cut(s) 151, 194, 430, 673, 1531, 1544
Hpy8I GTNNAC 5 cut(s) 84, 177, 313, 883, 1327
Hpy99I CGWCG 1 cut(s) 531
HpyAV CCTTC 5 cut(s) 278, 361, 383, 1304, 1492
HpyCH4III ACNGT 3 cut(s) 181, 317, 426
HpyCH4IV ACGT 1 cut(s) 1254
HpyCH4V TGCA 8 cut(s) 204, 593, 706, 830, 926, 1145, 1518, 1654
HpyF10VI GCNNNNNNNGC 2 cut(s) 1187, 1521
HpyF3I CTNAG 3 cut(s) 302, 534, 1008
HpySE526I ACGT 1 cut(s) 1254
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 8 cut(s) 91, 593, 656, 676, 1136, 1166, 1463, 1555
HspAI GCGC 2 cut(s) 129, 1513
KflI GGGWCCC 1 cut(s) 1158
Kpn2I TCCGGA 1 cut(s) 150
KroI GCCGGC 2 cut(s) 32, 110
KroNI GCCGGC 2 cut(s) 34, 112
Kzo9I GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
LmnI GCTCC 2 cut(s) 903, 1510
Lsp1109I GCAGC 4 cut(s) 842, 974, 1174, 1502
LweI GCATC 7 cut(s) 213, 487, 569, 1102, 1132, 1245, 1527
MaeI CTAG 4 cut(s) 194, 564, 872, 1083
MaeII ACGT 1 cut(s) 1254
MaeIII GTNAC 4 cut(s) 52, 1168, 1255, 1385
MalI GATC 7 cut(s) 72, 352, 629, 990, 1092, 1288, 1376
MbiI CCGCTC 1 cut(s) 906
MboI GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
MboII GAAGA 6 cut(s) 166, 261, 264, 1112, 1262, 1493
MflI RGATCY 2 cut(s) 627, 1374
MhlI GDGCHC 2 cut(s) 535, 680
MluCI AATT 8 cut(s) 209, 220, 227, 771, 1043, 1152, 1598, 1649
MluI ACGCGT 1 cut(s) 447
MmeI TCCRAC 3 cut(s) 39, 957, 1345
Mph1103I ATGCAT 2 cut(s) 206, 1147
MroI TCCGGA 1 cut(s) 150
MroNI GCCGGC 2 cut(s) 32, 110
MroXI GAANNNNTTC 2 cut(s) 161, 646
MseI TTAA 5 cut(s) 441, 791, 941, 1118, 1395
MslI CAYNNNNRTG 2 cut(s) 651, 711
MspA1I CMGCKG 2 cut(s) 611, 965
MspI CCGG 4 cut(s) 33, 111, 151, 189
MspR9I CCNGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
Mva1269I GAATGC 2 cut(s) 206, 854
MvaI CCWGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
MvnI CGCG 3 cut(s) 129, 449, 473
MwoI GCNNNNNNNGC 2 cut(s) 1187, 1521
NaeI GCCGGC 2 cut(s) 34, 112
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 2 cut(s) 889, 1414
NdeII GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
NgoMIV GCCGGC 2 cut(s) 32, 110
NlaIII CATG 8 cut(s) 91, 593, 656, 676, 1136, 1166, 1463, 1555
NlaIV GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1638
NmeAIII GCCGAG 1 cut(s) 259
NmuCI GTSAC 2 cut(s) 52, 1385
NsiI ATGCAT 2 cut(s) 206, 1147
NspI RCATGY 1 cut(s) 656
PaeR7I CTCGAG 1 cut(s) 512
PagI TCATGA 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 206, 854
PdiI GCCGGC 2 cut(s) 34, 112
PdmI GAANNNNTTC 2 cut(s) 161, 646
PfeI GAWTC 7 cut(s) 157, 288, 433, 497, 909, 980, 1400
PflMI CCANNNNNTGG 1 cut(s) 1364
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 9 cut(s) 7, 170, 472, 559, 610, 832, 964, 1189, 1517
PpuMI RGGWCCY 3 cut(s) 392, 539, 1158
Psp124BI GAGCTC 1 cut(s) 535
Psp5II RGGWCCY 3 cut(s) 392, 539, 1158
Psp6I CCWGG 6 cut(s) 21, 342, 747, 849, 1054, 1320
PspGI CCWGG 6 cut(s) 21, 342, 747, 849, 1054, 1320
PspN4I GGNNCC 8 cut(s) 37, 372, 540, 629, 1159, 1160, 1376, 1638
PspPI GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
PspPPI RGGWCCY 3 cut(s) 392, 539, 1158
PspXI VCTCGAGB 1 cut(s) 512
PsrI GAACNNNNNNTAC 2 cut(s) 167, 199
PsuI RGATCY 2 cut(s) 627, 1374
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 8 cut(s) 176, 569, 727, 799, 956, 1053, 1422, 1604
RsaNI GTAC 8 cut(s) 175, 568, 726, 798, 955, 1052, 1421, 1603
RseI CAYNNNNRTG 2 cut(s) 651, 711
SacI GAGCTC 1 cut(s) 535
SaqAI TTAA 5 cut(s) 441, 791, 941, 1118, 1395
SatI GCNGC 9 cut(s) 6, 169, 471, 558, 609, 831, 963, 1188, 1516
Sau3AI GATC 7 cut(s) 70, 350, 627, 988, 1090, 1286, 1374
Sau96I GGNCC 7 cut(s) 25, 35, 392, 539, 1129, 1158, 1463
ScaI AGTACT 1 cut(s) 727
ScrFI CCNGG 6 cut(s) 23, 344, 749, 851, 1056, 1322
SduI GDGCHC 2 cut(s) 535, 680
SfaNI GCATC 7 cut(s) 213, 487, 569, 1102, 1132, 1245, 1527
Sfr274I CTCGAG 1 cut(s) 512
SinI GGWCC 3 cut(s) 392, 539, 1158
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 2 cut(s) 651, 711
SmlI CTYRAG 3 cut(s) 512, 1182, 1529
SmoI CTYRAG 3 cut(s) 512, 1182, 1529
Sse9I AATT 8 cut(s) 209, 220, 227, 771, 1043, 1152, 1598, 1649
SsiI CCGC 8 cut(s) 5, 168, 256, 471, 557, 609, 632, 906
SspMI CTAG 4 cut(s) 194, 564, 872, 1083
SstI GAGCTC 1 cut(s) 535
StyD4I CCNGG 6 cut(s) 21, 342, 747, 849, 1054, 1320
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 181, 317, 426
TaiI ACGT 1 cut(s) 1257
TaqI TCGA 5 cut(s) 246, 327, 417, 513, 529
TasI AATT 8 cut(s) 209, 220, 227, 771, 1043, 1152, 1598, 1649
TatI WGTACW 2 cut(s) 174, 725
TauI GCSGC 5 cut(s) 8, 171, 473, 560, 611
TfiI GAWTC 7 cut(s) 157, 288, 433, 497, 909, 980, 1400
Tru1I TTAA 5 cut(s) 441, 791, 941, 1118, 1395
Tru9I TTAA 5 cut(s) 441, 791, 941, 1118, 1395
TscAI CASTG 4 cut(s) 184, 708, 718, 782
TseFI GTSAC 2 cut(s) 52, 1385
TseI GCWGC 4 cut(s) 830, 962, 1187, 1515
Tsp45I GTSAC 2 cut(s) 52, 1385
TspDTI ATGAA 8 cut(s) 202, 578, 661, 1113, 1362, 1413, 1460, 1600
TspGWI ACGGA 3 cut(s) 150, 1387, 1563
TspRI CASTG 4 cut(s) 184, 708, 718, 782
Van91I CCANNNNNTGG 1 cut(s) 1364
VpaK11BI GGWCC 3 cut(s) 392, 539, 1158
XapI RAATTY 3 cut(s) 209, 771, 1043
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XhoI CTCGAG 1 cut(s) 512
XmiI GTMKAC 2 cut(s) 882, 1326
XmnI GAANNNNTTC 2 cut(s) 161, 646
XspI CTAG 4 cut(s) 194, 564, 872, 1083
ZrmI AGTACT 1 cut(s) 727
Zsp2I ATGCAT 2 cut(s) 206, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.