RchiOBHm_Chr7g0236011

Nicotinate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005674
Physical Location & Seq
Forward (+)
60883139 .. 60884791
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21150

Sequence Viewer

Length: 1653 bp
ATGGTCCTCGCCGGCCCCACCAATCCAATGGTCACGCCTCTCCTCACCGATCTCTACCAGTTCACCATGGCCTACGCTTACTGGAAAGCCGGCAAGCACAAGGAACGAGCCGTGTTTGATTTGTATTTCCGCAAGAATCCATTCGGCGGCGAATACACAGTGTTTGCTGGATTAGAGGAATGCATCAAATTCATTGCTAATTTCAAACTGGCCGACGACGAAATCGATTTCGTCCGCCAATCCTTGTCTACTTCATGTGAGCTAGAGGAAGGATTCTATAAGTATCTGAGTGGAGTTGACTGTTCCGAAGTTGAAGTCTATGCTATCCAAGAAGGATCAGTCGTTTTTCCAAAGGTTCCTCTCGTTAGAGTTGAAGGACCAATCGCTGTGGTTCAGTTACTCGAAACTCCATTCTTGAATCTCATTAATTATGCGTCATTAGTTGCTACAAATGCCGCAAGGCATCGTTACGTTGCTGGAGAATCTAAAATGCTACTTGAGTTTGGGCTTCGACGAGCTCAGGGACCTGATGGTGGAATAGGGGCATCTAGGTACTGCTACATGGGAGGATTTCATGCAACAAGTAATGTTGCCGCTGGAAAGATATTTGGGATCCCGCTTCGCGGAACACATTCGCATGCTTTTGTCAGCTCATTCATGAGCCCAGACGAGATTATAGATAAATCACTTCGTCATAGCAGTGATAAGCGCAGTACTTGTGAGGATTTTGTTAGCCTGGTTCAGACTTGGTTGCGCAAAATTCAGTCGTGGTCAAATTCATTAAATGGTACTTTTGGTGGTGAGACCAATCATAGTGAGCTTGCAGCATTCACATCGTATGCCCTGGCATTCCCTGATAACTTTCTAGCTCTTGTAGACACATATGATGTTATGAGGAGTGGAATTCCCAACTTTTGTGCGGTTGCTTTAGCACTCAATGATCTAGGGTACAAAGCAGCTGGCATTAGGTTGGATTCAGGTGATCTAGCATATCTCTCCTCTGAGGCTCGAAAGATTTTTCTCATCGTTGAGAAAGAATTTGGAGTACCTGGATTTGGAAAGACGGTTATCACAGCTAGTAATGATCTAAATGAGGAAACTTTGGATGCTTTAAACAAACAGCGCCATGAGGTTGATGCATTTGGAATTGGGACCCATGTAGTTACTTGTTATGCTCAAGCTGCTCTGGGTTGTGTTTTCAAGCTTGTTGAGATAAATAATCAGCCTCGCATCAAACTTTCTGAAGATGTCACAAAGGTCTCCATCCCTTGTAAAAAACGATCGTACAGATTGTTTGGGAAAGAAGGTTATCCCCTGGTAGACATAATGACCGGAGAAAATGAACCACCTCCAAAGGTTGGAGAACGGATCCTGTGTCGTCACCCCTTTAATGAATCGAAGAGAGCATATGTAGTGCCGCAGCGTGTTGAGGAACTTATGAAATGCTTTTGGCATGGCCCAGGTGGAGTGAGAGAAGATCTGCCACCATTAAAGGACATCAGGGAGCGCTGCATCAGCCAACTCGGGAAAATGCGCCCCGACCACATGAGGAGACTAAACCCCACTCCGTATAAGGTTAGTGTAAGTGCAAAACTGTATGACTTCATTCACTTCCTATGGCTCAATGAGGCACCTGTCGGGGAGTTGCAGTGA

Protein Analysis

550

Amino Acids

61.41

Weight (kDa)

6.52

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAPRTase_N PF17767 14 - 143 4e-38 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
QRPTase_N PF02749 103 - 146 5.7e-06 Quinolinate phosphoribosyl transferase, N-terminal domain
NAPRTase_C PF17956 425 - 535 1.5e-31 Nicotinate phosphoribosyltransferase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 1630
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccI GTMKAC 3 cut(s) 248, 876, 1320
AccII CGCG 1 cut(s) 624
AciI CCGC 9 cut(s) 130, 147, 235, 456, 594, 617, 624, 920, 1418
AclWI GGATC 5 cut(s) 343, 607, 620, 1363, 1376
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 5 cut(s) 188, 759, 775, 903, 1037
AcuI CTGAAG 1 cut(s) 1263
AfaI GTAC 6 cut(s) 554, 715, 790, 950, 1047, 1286
AfeI AGCGCT 1 cut(s) 1508
AfiI CCNNNNNNNGG 5 cut(s) 146, 533, 623, 1055, 1358
AgsI TTSAA 5 cut(s) 205, 314, 374, 418, 1201
AjnI CCWGG 5 cut(s) 735, 843, 1048, 1314, 1459
AluBI AGCT 9 cut(s) 262, 518, 651, 820, 869, 959, 1076, 1181, 1204
AluI AGCT 9 cut(s) 262, 518, 651, 820, 869, 959, 1076, 1181, 1204
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 3 cut(s) 797, 1264, 1546
AlwI GGATC 5 cut(s) 343, 607, 620, 1363, 1376
Ama87I CYCGRG 1 cut(s) 1523
Aor51HI AGCGCT 1 cut(s) 1508
AoxI GGCC 4 cut(s) 13, 69, 210, 1456
ApeKI GCWGC 5 cut(s) 824, 956, 1181, 1420, 1509
ApoI RAATTY 5 cut(s) 188, 759, 775, 903, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AseI ATTAAT 1 cut(s) 426
Asp700I GAANNNNTTC 2 cut(s) 140, 631
AspLEI GCGC 5 cut(s) 711, 756, 1125, 1509, 1536
AspS9I GGNCC 6 cut(s) 4, 14, 377, 524, 1152, 1457
AsuHPI GGTGA 5 cut(s) 37, 55, 812, 992, 1373
AvaI CYCGRG 1 cut(s) 1523
AvaII GGWCC 4 cut(s) 4, 377, 524, 1152
BamHI GGATCC 2 cut(s) 612, 1368
BanI GGYRCC 1 cut(s) 1630
BanII GRGCYC 2 cut(s) 520, 665
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 5 cut(s) 836, 968, 1168, 1432, 1496
BccI CCATC 2 cut(s) 524, 1271
BceAI ACGGC 1 cut(s) 95
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BciT130I CCWGG 5 cut(s) 737, 845, 1050, 1316, 1461
BcoDI GTCTC 3 cut(s) 797, 1264, 1546
BfaI CTAG 6 cut(s) 263, 549, 866, 944, 986, 1077
BfoI RGCGCY 2 cut(s) 1126, 1510
BglII AGATCT 1 cut(s) 1477
BisI GCNGC 9 cut(s) 148, 456, 594, 825, 957, 1182, 1418, 1421, 1510
BlsI GCNGC 9 cut(s) 149, 457, 595, 826, 958, 1183, 1419, 1422, 1511
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 5 cut(s) 737, 845, 1050, 1316, 1461
Bme18I GGWCC 4 cut(s) 4, 377, 524, 1152
BmeT110I CYCGRG 1 cut(s) 1523
BmgT120I GGNCC 6 cut(s) 4, 14, 377, 524, 1152, 1457
BmiI GGNNCC 8 cut(s) 16, 357, 525, 614, 1153, 1154, 1370, 1632
BmrFI CCNGG 5 cut(s) 737, 845, 1050, 1316, 1461
BmsI GCATC 7 cut(s) 192, 472, 554, 1096, 1126, 1239, 1521
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 2 cut(s) 518, 1161
Bsa29I ATCGAT 1 cut(s) 225
BsaI GGTCTC 2 cut(s) 797, 1264
BsaJI CCNNGG 4 cut(s) 66, 843, 1314, 1459
BsaWI WCCGGW 1 cut(s) 1331
BsaXI ACNNNNNCTCC 1 cut(s) 1634
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 533, 623, 1055, 1358
Bse118I RCCGGY 2 cut(s) 11, 89
Bse1I ACTGG 3 cut(s) 58, 86, 213
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 5 cut(s) 737, 845, 1050, 1316, 1461
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 4 cut(s) 66, 843, 1314, 1459
BseGI GGATG 2 cut(s) 1111, 1263
BseLI CCNNNNNNNGG 5 cut(s) 146, 533, 623, 1055, 1358
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 3 cut(s) 278, 533, 993
BseNI ACTGG 3 cut(s) 58, 86, 213
BseRI GAGGAG 4 cut(s) 32, 910, 988, 1564
BseXI GCAGC 5 cut(s) 836, 968, 1168, 1432, 1496
Bsh1236I CGCG 1 cut(s) 624
Bsh1285I CGRYCG 1 cut(s) 1283
BshFI GGCC 4 cut(s) 15, 71, 212, 1458
BshNI GGYRCC 1 cut(s) 1630
BshVI ATCGAT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 1283
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 1523
BsiSI CCGG 3 cut(s) 12, 90, 1332
BslFI GGGAC 2 cut(s) 537, 1165
BslI CCNNNNNNNGG 5 cut(s) 146, 533, 623, 1055, 1358
BsmAI GTCTC 3 cut(s) 797, 1264, 1546
BsmFI GGGAC 2 cut(s) 537, 1165
BsmI GAATGC 3 cut(s) 185, 827, 848
BsnI GGCC 4 cut(s) 15, 71, 212, 1458
Bso31I GGTCTC 2 cut(s) 797, 1264
BsoBI CYCGRG 1 cut(s) 1523
Bsp1286I GDGCHC 2 cut(s) 520, 665
Bsp143I GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 9 cut(s) 130, 147, 235, 456, 594, 617, 624, 920, 1418
BspANI GGCC 4 cut(s) 15, 71, 212, 1458
BspCNI CTCAG 3 cut(s) 279, 532, 994
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 1 cut(s) 624
BspHI TCATGA 1 cut(s) 657
BspLI GGNNCC 8 cut(s) 16, 357, 525, 614, 1153, 1154, 1370, 1632
BspPI GGATC 5 cut(s) 343, 607, 620, 1363, 1376
BspT107I GGYRCC 1 cut(s) 1630
BspTNI GGTCTC 2 cut(s) 797, 1264
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 11, 89
BsrI ACTGG 3 cut(s) 58, 86, 213
BssAI RCCGGY 2 cut(s) 11, 89
BssECI CCNNGG 4 cut(s) 66, 843, 1314, 1459
BssMI GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 5 cut(s) 737, 845, 1050, 1316, 1461
Bst4CI ACNGT 4 cut(s) 160, 302, 1066, 1596
Bst6I CTCTTC 1 cut(s) 1394
BstC8I GCNNGC 6 cut(s) 13, 91, 95, 639, 822, 961
BstDEI CTNAG 3 cut(s) 287, 519, 1002
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 1111, 1263
BstFNI CGCG 1 cut(s) 624
BstH2I RGCGCY 2 cut(s) 1126, 1510
BstHHI GCGC 5 cut(s) 711, 756, 1125, 1509, 1536
BstKTI GATC 9 cut(s) 52, 338, 615, 943, 985, 1087, 1283, 1371, 1480
BstMAI GTCTC 3 cut(s) 797, 1264, 1546
BstMBI GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
BstMCI CGRYCG 1 cut(s) 1283
BstMWI GCNNNNNNNGC 3 cut(s) 452, 1181, 1515
BstNI CCWGG 5 cut(s) 737, 845, 1050, 1316, 1461
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 5 cut(s) 735, 843, 1048, 1314, 1459
BstUI CGCG 1 cut(s) 624
BstV1I GCAGC 5 cut(s) 836, 968, 1168, 1432, 1496
BstX2I RGATCY 3 cut(s) 612, 1368, 1477
BstXI CCANNNNNNTGG 1 cut(s) 28
BstYI RGATCY 3 cut(s) 612, 1368, 1477
Bsu15I ATCGAT 1 cut(s) 225
BsuRI GGCC 4 cut(s) 15, 71, 212, 1458
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 1111, 1263
BtsI GCAGTG 1 cut(s) 706
BtsIMutI CAGTG 2 cut(s) 165, 706
Cac8I GCNNGC 6 cut(s) 13, 91, 95, 639, 822, 961
CciI TCATGA 1 cut(s) 657
CfoI GCGC 5 cut(s) 711, 756, 1125, 1509, 1536
Cfr10I RCCGGY 2 cut(s) 11, 89
Cfr13I GGNCC 6 cut(s) 4, 14, 377, 524, 1152, 1457
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 1 cut(s) 423
Csp6I GTAC 6 cut(s) 553, 714, 789, 949, 1046, 1285
CspCI CAANNNNNGTGG 2 cut(s) 369, 404
CviQI GTAC 6 cut(s) 553, 714, 789, 949, 1046, 1285
DdeI CTNAG 3 cut(s) 287, 519, 1002
DpnI GATC 9 cut(s) 51, 337, 614, 942, 984, 1086, 1282, 1370, 1479
DpnII GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
DraI TTTAAA 1 cut(s) 1113
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 1394
EarI CTCTTC 1 cut(s) 1394
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 1 cut(s) 518
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 2 cut(s) 520, 665
Eco31I GGTCTC 2 cut(s) 797, 1264
Eco47I GGWCC 4 cut(s) 4, 377, 524, 1152
Eco47III AGCGCT 1 cut(s) 1508
Eco53kI GAGCTC 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 1263
Eco88I CYCGRG 1 cut(s) 1523
EcoICRI GAGCTC 1 cut(s) 518
EcoO109I RGGNCCY 2 cut(s) 524, 1152
EcoRI GAATTC 1 cut(s) 903
EcoRII CCWGG 5 cut(s) 735, 843, 1048, 1314, 1459
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 2 cut(s) 185, 1141
EcoT38I GRGCYC 2 cut(s) 520, 665
ErhI CCWWGG 1 cut(s) 66
FaqI GGGAC 2 cut(s) 537, 1165
FauI CCCGC 1 cut(s) 624
FauNDI CATATG 2 cut(s) 883, 1408
FblI GTMKAC 3 cut(s) 248, 876, 1320
Fnu4HI GCNGC 9 cut(s) 148, 456, 594, 825, 957, 1182, 1418, 1421, 1510
FokI GGATG 2 cut(s) 1118, 1250
FriOI GRGCYC 2 cut(s) 520, 665
Fsp4HI GCNGC 9 cut(s) 148, 456, 594, 825, 957, 1182, 1418, 1421, 1510
FspBI CTAG 6 cut(s) 263, 549, 866, 944, 986, 1077
FspI TGCGCA 1 cut(s) 755
GlaI GCGC 5 cut(s) 710, 755, 1124, 1508, 1535
GluI GCNGC 9 cut(s) 148, 456, 594, 825, 957, 1182, 1418, 1421, 1510
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 2 cut(s) 1126, 1510
HaeIII GGCC 4 cut(s) 15, 71, 212, 1458
HapII CCGG 3 cut(s) 12, 90, 1332
HgaI GACGC 1 cut(s) 423
HhaI GCGC 5 cut(s) 711, 756, 1125, 1509, 1536
Hin6I GCGC 5 cut(s) 709, 754, 1123, 1507, 1534
HinP1I GCGC 5 cut(s) 709, 754, 1123, 1507, 1534
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 1202
HinfI GANTC 6 cut(s) 136, 273, 418, 482, 974, 1394
HpaII CCGG 3 cut(s) 12, 90, 1332
HphI GGTGA 5 cut(s) 37, 55, 812, 992, 1373
Hpy166II GTNNAC 5 cut(s) 63, 249, 298, 877, 1321
Hpy188I TCNGA 5 cut(s) 288, 307, 744, 1003, 1243
Hpy188III TCNNGA 3 cut(s) 415, 658, 1525
Hpy8I GTNNAC 5 cut(s) 63, 249, 298, 877, 1321
Hpy99I CGWCG 3 cut(s) 218, 221, 516
HpyAV CCTTC 4 cut(s) 263, 326, 368, 1298
HpyCH4III ACNGT 4 cut(s) 160, 302, 1066, 1596
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 7 cut(s) 183, 578, 824, 1139, 1512, 1589, 1648
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 1181, 1515
HpyF3I CTNAG 3 cut(s) 287, 519, 1002
HpySE526I ACGT 1 cut(s) 471
HspAI GCGC 5 cut(s) 709, 754, 1123, 1507, 1534
KflI GGGWCCC 1 cut(s) 1152
KroI GCCGGC 2 cut(s) 11, 89
KroNI GCCGGC 2 cut(s) 13, 91
Kzo9I GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
LmnI GCTCC 1 cut(s) 1504
Lsp1109I GCAGC 5 cut(s) 836, 968, 1168, 1432, 1496
LweI GCATC 7 cut(s) 192, 472, 554, 1096, 1126, 1239, 1521
MaeI CTAG 6 cut(s) 263, 549, 866, 944, 986, 1077
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 6 cut(s) 31, 396, 467, 1162, 1249, 1379
MalI GATC 9 cut(s) 51, 337, 614, 942, 984, 1086, 1282, 1370, 1479
MboI GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
MboII GAAGA 3 cut(s) 1256, 1411, 1487
MflI RGATCY 3 cut(s) 612, 1368, 1477
MhlI GDGCHC 2 cut(s) 520, 665
MluCI AATT 8 cut(s) 188, 199, 427, 759, 775, 903, 1037, 1146
MmeI TCCRAC 2 cut(s) 951, 1339
Mph1103I ATGCAT 2 cut(s) 185, 1141
MroNI GCCGGC 2 cut(s) 11, 89
MroXI GAANNNNTTC 2 cut(s) 140, 631
MseI TTAA 5 cut(s) 426, 782, 1112, 1389, 1490
MslI CAYNNNNRTG 2 cut(s) 636, 699
MspA1I CMGCKG 2 cut(s) 596, 959
MspI CCGG 3 cut(s) 12, 90, 1332
MspR9I CCNGG 5 cut(s) 737, 845, 1050, 1316, 1461
Mva1269I GAATGC 3 cut(s) 185, 827, 848
MvaI CCWGG 5 cut(s) 737, 845, 1050, 1316, 1461
MvnI CGCG 1 cut(s) 624
MwoI GCNNNNNNNGC 3 cut(s) 452, 1181, 1515
NaeI GCCGGC 2 cut(s) 13, 91
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 2 cut(s) 883, 1408
NdeII GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
NgoMIV GCCGGC 2 cut(s) 11, 89
NlaIV GGNNCC 8 cut(s) 16, 357, 525, 614, 1153, 1154, 1370, 1632
NmuCI GTSAC 3 cut(s) 31, 1249, 1379
NsbI TGCGCA 1 cut(s) 755
NsiI ATGCAT 2 cut(s) 185, 1141
NspI RCATGY 1 cut(s) 641
PaeI GCATGC 1 cut(s) 641
PagI TCATGA 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 3 cut(s) 185, 827, 848
PdiI GCCGGC 2 cut(s) 13, 91
PdmI GAANNNNTTC 2 cut(s) 140, 631
PfeI GAWTC 6 cut(s) 136, 273, 418, 482, 974, 1394
PflMI CCANNNNNTGG 1 cut(s) 1358
PkrI GCNGC 9 cut(s) 149, 457, 595, 826, 958, 1183, 1419, 1422, 1511
Ple19I CGATCG 1 cut(s) 1283
PpuMI RGGWCCY 2 cut(s) 524, 1152
PshBI ATTAAT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 520
Psp5II RGGWCCY 2 cut(s) 524, 1152
Psp6I CCWGG 5 cut(s) 735, 843, 1048, 1314, 1459
PspGI CCWGG 5 cut(s) 735, 843, 1048, 1314, 1459
PspN4I GGNNCC 8 cut(s) 16, 357, 525, 614, 1153, 1154, 1370, 1632
PspPI GGNCC 6 cut(s) 4, 14, 377, 524, 1152, 1457
PspPPI RGGWCCY 2 cut(s) 524, 1152
PsuI RGATCY 3 cut(s) 612, 1368, 1477
PvuI CGATCG 1 cut(s) 1283
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 6 cut(s) 554, 715, 790, 950, 1047, 1286
RsaNI GTAC 6 cut(s) 553, 714, 789, 949, 1046, 1285
RseI CAYNNNNRTG 2 cut(s) 636, 699
SacI GAGCTC 1 cut(s) 520
SaqAI TTAA 5 cut(s) 426, 782, 1112, 1389, 1490
SatI GCNGC 9 cut(s) 148, 456, 594, 825, 957, 1182, 1418, 1421, 1510
Sau3AI GATC 9 cut(s) 49, 335, 612, 940, 982, 1084, 1280, 1368, 1477
Sau96I GGNCC 6 cut(s) 4, 14, 377, 524, 1152, 1457
ScaI AGTACT 1 cut(s) 715
ScrFI CCNGG 5 cut(s) 737, 845, 1050, 1316, 1461
SduI GDGCHC 2 cut(s) 520, 665
SfaNI GCATC 7 cut(s) 192, 472, 554, 1096, 1126, 1239, 1521
SinI GGWCC 4 cut(s) 4, 377, 524, 1152
SmiMI CAYNNNNRTG 2 cut(s) 636, 699
SmlI CTYRAG 2 cut(s) 497, 1176
SmoI CTYRAG 2 cut(s) 497, 1176
SphI GCATGC 1 cut(s) 641
Sse9I AATT 8 cut(s) 188, 199, 427, 759, 775, 903, 1037, 1146
SsiI CCGC 9 cut(s) 130, 147, 235, 456, 594, 617, 624, 920, 1418
SspMI CTAG 6 cut(s) 263, 549, 866, 944, 986, 1077
SstI GAGCTC 1 cut(s) 520
StyD4I CCNGG 5 cut(s) 735, 843, 1048, 1314, 1459
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 4 cut(s) 160, 302, 1066, 1596
TaiI ACGT 1 cut(s) 474
TaqI TCGA 5 cut(s) 225, 402, 511, 1009, 1397
TasI AATT 8 cut(s) 188, 199, 427, 759, 775, 903, 1037, 1146
TatI WGTACW 1 cut(s) 713
TauI GCSGC 4 cut(s) 150, 458, 596, 1420
TfiI GAWTC 6 cut(s) 136, 273, 418, 482, 974, 1394
Tru1I TTAA 5 cut(s) 426, 782, 1112, 1389, 1490
Tru9I TTAA 5 cut(s) 426, 782, 1112, 1389, 1490
TscAI CASTG 2 cut(s) 165, 706
TseFI GTSAC 3 cut(s) 31, 1249, 1379
TseI GCWGC 5 cut(s) 824, 956, 1181, 1420, 1509
Tsp45I GTSAC 3 cut(s) 31, 1249, 1379
TspDTI ATGAA 9 cut(s) 181, 243, 563, 646, 768, 1356, 1407, 1454, 1594
TspGWI ACGGA 2 cut(s) 1381, 1557
TspRI CASTG 2 cut(s) 165, 706
Van91I CCANNNNNTGG 1 cut(s) 1358
VpaK11BI GGWCC 4 cut(s) 4, 377, 524, 1152
VspI ATTAAT 1 cut(s) 426
XapI RAATTY 5 cut(s) 188, 759, 775, 903, 1037
XceI RCATGY 1 cut(s) 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 3 cut(s) 248, 876, 1320
XmnI GAANNNNTTC 2 cut(s) 140, 631
XspI CTAG 6 cut(s) 263, 549, 866, 944, 986, 1077
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 2 cut(s) 185, 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.