AT2G23890

5' nucleotidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006733
Physical Location & Seq
Forward (+)
10170576 .. 10173945
3370 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23890.3

Sequence Viewer

Length: 1662 bp
ATGACTTCCTCTCGCCGTCTTCTTCTTCCTCTCGGAGCATCGCTCACTAGAGGAAGATTTTCTTCCGATCAAATCCGAAATGGATTTCTAAGAAACTTCCGTGGATTCGCCACCGTAACTTCGTCGGAACCGGCCTTAGCCAATCTGGAAGCGAAATATGCCGTAGCGTTGCCAGAATGTTCAACAGTAGAGGACGAGATCACGAAGATCCGTCATGAATTCGAGTTAGCGAAACAGAGGTTTCTTAATATCCCTGAAGCTATTAATAGTATGCCGAAGATGAATCCTCAAGGGATATATGTGAATAAGAATCTGAGATTGGATAATATACAAGTTTATGGATTTGATTATGATTACACTTTGGCACATTACTCTTCTCACTTACAGAGTTTGATCTATGATCTTGCCAAGAAACATATGGTTAATGAGTTTAGATATCCTGATGTTTGCACTCAGTTTGAGTATGATCCTACTTTTCCAATCCGTGGGTTGTACTATGATAAACTAAAAGGATGCCTCATGAAATTGGATTTCTTCGGTTCAATCGAGCCAGATGGGTGTTATTTTGGTCGTCGTAAGCTTAGTAGGAAGGAAATAGAAAGCATGTATGGAACGCGGCACATAGGTCGTGATCAAGCGAGAGGTTTGGTGGGATTGATGGATTTCTTCTGTTTTAGCGAGGCGTGTCTTATAGCAGACATGGTGCAATATTTTGTTGACGCCAAACTTGAGTTTGATGCCTCTAACATCTACAATGATGTCAATCGTGCTATTCAACATGTCCATAGAAGTGGATTGGTTCATAGAGGAATTCTTGCTGATCCCAACAGATATTTGCTAAAAAATGGTCAGCTTCTACGTTTCCTGAGAATGCTAAAAGATAAAGGAAAGAAGCTTTTTTTGCTGACCAACTCTCCGTATAATTTTGTTGATGGCGGAATGCGCTTTCTAATGGAGGAATCTTTTGGCTTCGGAGATTCCTGGCGAGAACTCTTTGATGTTGTGATTGCTAAAGCAAATAAACCAGAATTTTACACATCTGAGCACCCTTTCCGTTGTTATGATTCGGAGAGGGATAATTTGGCATTTACAAAAGTGGATGCATTTGACCCAAAGAAAGTTTATTATCATGGTTGTCTTAAATCCTTCCTTGAAATCACAAAGTGGCATGGCCCTGAGGTGATTTATTTCGGAGATCACTTATTTAGTGATCTAAGAGGGCCTTCAAAAGCTGGTTGGCGAACTGCTGCCATAATTCATGAGCTCGAGCGAGAGATACAGATACAAAATGATGATAGCTACCGGTTTGAGCAGGCCAAGTTCCATATTATCCAAGAGTTACTCGGTAGATTTCACGCGACTGTATCAAACAATCAGAGAAGTGAAGCATGCCAATCACTTTTGGATGAGCTGAACAATGCGAGGCAGAGAGCAAGAGACACGATGAAACAAATGTTCAACAGATCGTTTGGAGCTACATTTGTCACAGACACTGGTCAAGAATCAGCATTCTCTTATCACATCCACCAATACGCAGACGTTTATACCAGTAAACCTGAGAACTTTCTGTTATACCGACCTGAAGCCTGGCTTCACGTTCCTTACGATATCAAGATCATGCCACATCATGTCAAGGTTGCTTCAACCCTTTTCAAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

553

Amino Acids

64.31

Weight (kDa)

8.2

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 9 - 454 7e-151 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 485
AccII CGCG 2 cut(s) 616, 1358
AciI CCGC 2 cut(s) 616, 936
AclWI GGATC 3 cut(s) 202, 461, 815
AcsI RAATTY 3 cut(s) 218, 810, 1028
AcuI CTGAAG 2 cut(s) 276, 1602
AcyI GRCGYC 1 cut(s) 720
AdeI CACNNNGTG 1 cut(s) 1164
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 485
AflIII ACRYGT 1 cut(s) 778
AgeI ACCGGT 1 cut(s) 1302
AgsI TTSAA 8 cut(s) 183, 543, 776, 1154, 1227, 1459, 1644, 1654
AjnI CCWGG 2 cut(s) 980, 1586
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AluBI AGCT 9 cut(s) 260, 580, 853, 895, 1232, 1264, 1299, 1411, 1475
AluI AGCT 9 cut(s) 260, 580, 853, 895, 1232, 1264, 1299, 1411, 1475
Alw21I GWGCWC 2 cut(s) 1047, 1266
Alw26I GTCTC 1 cut(s) 1431
AlwI GGATC 3 cut(s) 202, 461, 815
AlwNI CAGNNNCTG 1 cut(s) 1493
Ama87I CYCGRG 1 cut(s) 1265
AoxI GGCC 4 cut(s) 132, 1171, 1220, 1314
ApeKI GCWGC 1 cut(s) 1247
ApoI RAATTY 3 cut(s) 218, 810, 1028
AseI ATTAAT 1 cut(s) 264
AsiGI ACCGGT 1 cut(s) 1302
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 1 cut(s) 945
AspS9I GGNCC 2 cut(s) 1172, 1220
AsuHPI GGTGA 1 cut(s) 1192
AvaI CYCGRG 1 cut(s) 1265
AxyI CCTNAGG 1 cut(s) 1176
BanII GRGCYC 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 1047, 1266
BbvI GCAGC 1 cut(s) 1234
BccI CCATC 3 cut(s) 548, 652, 926
BceAI ACGGC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 2 cut(s) 982, 1588
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 1 cut(s) 1431
BfaI CTAG 1 cut(s) 48
BisI GCNGC 2 cut(s) 617, 1248
BlsI GCNGC 2 cut(s) 618, 1249
Bme1390I CCNGG 2 cut(s) 982, 1588
BmeT110I CYCGRG 1 cut(s) 1265
BmgT120I GGNCC 2 cut(s) 1172, 1220
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 2 cut(s) 982, 1588
BmsI GCATC 4 cut(s) 47, 503, 727, 1090
BoxI GACNNNNGTC 1 cut(s) 1494
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 27, 59
Bpu10I CCTNAGC 1 cut(s) 136
BpuEI CTTGAG 2 cut(s) 273, 749
BsaBI GATNNNNATC 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 720
BsaJI CCNNGG 2 cut(s) 100, 484
BsaWI WCCGGW 1 cut(s) 1302
BsaXI ACNNNNNCTCC 2 cut(s) 898, 928
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse118I RCCGGY 2 cut(s) 130, 1302
Bse1I ACTGG 2 cut(s) 1498, 1548
Bse21I CCTNAGG 1 cut(s) 1176
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 2 cut(s) 982, 1588
BseDI CCNNGG 2 cut(s) 100, 484
BseGI GGATG 4 cut(s) 518, 1105, 1411, 1521
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 6 cut(s) 305, 467, 857, 1032, 1167, 1548
BseNI ACTGG 2 cut(s) 1498, 1548
BseXI GCAGC 1 cut(s) 1234
Bsh1236I CGCG 2 cut(s) 616, 1358
BshFI GGCC 4 cut(s) 134, 1173, 1222, 1316
BshTI ACCGGT 1 cut(s) 1302
BsiHKAI GWGCWC 2 cut(s) 1047, 1266
BsiHKCI CYCGRG 1 cut(s) 1265
BsiSI CCGG 2 cut(s) 131, 1303
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 1431
BsmI GAATGC 3 cut(s) 876, 945, 1508
BsnI GGCC 4 cut(s) 134, 1173, 1222, 1316
BsoBI CYCGRG 1 cut(s) 1265
Bsp1286I GDGCHC 2 cut(s) 1047, 1266
BspACI CCGC 2 cut(s) 616, 936
BspANI GGCC 4 cut(s) 134, 1173, 1222, 1316
BspCNI CTCAG 6 cut(s) 306, 466, 858, 1033, 1168, 1549
BspFNI CGCG 2 cut(s) 616, 1358
BspHI TCATGA 3 cut(s) 214, 519, 1258
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 3 cut(s) 202, 461, 815
BsrFI RCCGGY 2 cut(s) 130, 1302
BsrI ACTGG 2 cut(s) 1498, 1548
BssAI RCCGGY 2 cut(s) 130, 1302
BssECI CCNNGG 2 cut(s) 100, 484
BssNI GRCGYC 1 cut(s) 720
Bst2UI CCWGG 2 cut(s) 982, 1588
Bst4CI ACNGT 3 cut(s) 115, 187, 1363
Bst6I CTCTTC 1 cut(s) 379
BstACI GRCGYC 1 cut(s) 720
BstC8I GCNNGC 2 cut(s) 1314, 1390
BstDSI CCRYGG 2 cut(s) 100, 484
BstF5I GGATG 4 cut(s) 518, 1105, 1411, 1521
BstFNI CGCG 2 cut(s) 616, 1358
BstHHI GCGC 1 cut(s) 945
BstMAI GTCTC 1 cut(s) 1431
BstMWI GCNNNNNNNGC 3 cut(s) 158, 901, 942
BstNI CCWGG 2 cut(s) 982, 1588
BstNSI RCATGY 3 cut(s) 607, 782, 1392
BstPAI GACNNNNGTC 1 cut(s) 1494
BstSCI CCNGG 2 cut(s) 980, 1586
BstUI CGCG 2 cut(s) 616, 1358
BstV1I GCAGC 1 cut(s) 1234
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 1 cut(s) 207
Bsu36I CCTNAGG 1 cut(s) 1176
BsuRI GGCC 4 cut(s) 134, 1173, 1222, 1316
BtgI CCRYGG 2 cut(s) 100, 484
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 4 cut(s) 518, 1105, 1411, 1521
BtsIMutI CAGTG 1 cut(s) 1491
Cac8I GCNNGC 2 cut(s) 1314, 1390
CaiI CAGNNNCTG 1 cut(s) 1493
CciI TCATGA 3 cut(s) 214, 519, 1258
CfoI GCGC 1 cut(s) 945
Cfr10I RCCGGY 2 cut(s) 130, 1302
Cfr13I GGNCC 2 cut(s) 1172, 1220
CseI GACGC 1 cut(s) 728
Csp6I GTAC 1 cut(s) 493
CspAI ACCGGT 1 cut(s) 1302
CviQI GTAC 1 cut(s) 493
DraIII CACNNNGTG 1 cut(s) 1164
Eam1104I CTCTTC 1 cut(s) 379
EarI CTCTTC 1 cut(s) 379
EciI GGCGGA 1 cut(s) 951
Ecl136II GAGCTC 1 cut(s) 1264
Eco24I GRGCYC 1 cut(s) 1266
Eco32I GATATC 2 cut(s) 437, 1609
Eco53kI GAGCTC 1 cut(s) 1264
Eco57I CTGAAG 2 cut(s) 276, 1602
Eco81I CCTNAGG 1 cut(s) 1176
Eco88I CYCGRG 1 cut(s) 1265
EcoICRI GAGCTC 1 cut(s) 1264
EcoO109I RGGNCCY 1 cut(s) 1220
EcoRI GAATTC 2 cut(s) 218, 810
EcoRII CCWGG 2 cut(s) 980, 1586
EcoRV GATATC 2 cut(s) 437, 1609
EcoT22I ATGCAT 1 cut(s) 1105
EcoT38I GRGCYC 1 cut(s) 1266
FalI AAGNNNNNCTT 6 cut(s) 46, 78, 1207, 1239, 1575, 1607
FauNDI CATATG 1 cut(s) 417
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 2 cut(s) 617, 1248
FokI GGATG 4 cut(s) 525, 1112, 1418, 1508
FriOI GRGCYC 1 cut(s) 1266
Fsp4HI GCNGC 2 cut(s) 617, 1248
FspBI CTAG 1 cut(s) 48
GlaI GCGC 1 cut(s) 944
GluI GCNGC 2 cut(s) 617, 1248
HaeIII GGCC 4 cut(s) 134, 1173, 1222, 1316
HapII CCGG 2 cut(s) 131, 1303
HgaI GACGC 1 cut(s) 728
HhaI GCGC 1 cut(s) 945
Hin1I GRCGYC 1 cut(s) 720
Hin6I GCGC 1 cut(s) 943
HinP1I GCGC 1 cut(s) 943
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 2 cut(s) 578, 893
HinfI GANTC 7 cut(s) 105, 283, 310, 959, 977, 1064, 1502
HpaII CCGG 2 cut(s) 131, 1303
HphI GGTGA 1 cut(s) 1192
Hpy166II GTNNAC 2 cut(s) 718, 1553
Hpy8I GTNNAC 2 cut(s) 718, 1553
Hpy99I CGWCG 2 cut(s) 127, 576
HpyAV CCTTC 3 cut(s) 583, 1156, 1233
HpyCH4III ACNGT 3 cut(s) 115, 187, 1363
HpyCH4IV ACGT 3 cut(s) 859, 1539, 1596
HpyCH4V TGCA 3 cut(s) 450, 706, 1103
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 901, 942
HpySE526I ACGT 3 cut(s) 859, 1539, 1596
Hsp92I GRCGYC 1 cut(s) 720
HspAI GCGC 1 cut(s) 943
Ksp22I TGATCA 1 cut(s) 631
LmnI GCTCC 2 cut(s) 35, 1472
Lsp1109I GCAGC 1 cut(s) 1234
LweI GCATC 4 cut(s) 47, 503, 727, 1090
MaeI CTAG 1 cut(s) 48
MaeII ACGT 3 cut(s) 859, 1539, 1596
MaeIII GTNAC 3 cut(s) 115, 1338, 1483
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 1047, 1266
MluCI AATT 7 cut(s) 218, 524, 810, 922, 1028, 1078, 1254
MmeI TCCRAC 1 cut(s) 105
Mph1103I ATGCAT 1 cut(s) 1105
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 4 cut(s) 246, 264, 423, 1140
MslI CAYNNNNRTG 1 cut(s) 789
MspI CCGG 2 cut(s) 131, 1303
MspR9I CCNGG 2 cut(s) 982, 1588
Mva1269I GAATGC 3 cut(s) 876, 945, 1508
MvaI CCWGG 2 cut(s) 982, 1588
MvnI CGCG 2 cut(s) 616, 1358
MwoI GCNNNNNNNGC 3 cut(s) 158, 901, 942
NdeI CATATG 1 cut(s) 417
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 1483
NsiI ATGCAT 1 cut(s) 1105
NspI RCATGY 3 cut(s) 607, 782, 1392
PaeI GCATGC 1 cut(s) 1392
PaeR7I CTCGAG 1 cut(s) 1265
PagI TCATGA 3 cut(s) 214, 519, 1258
PciI ACATGT 1 cut(s) 778
PcsI WCGNNNNNNNCGW 2 cut(s) 543, 1602
PctI GAATGC 3 cut(s) 876, 945, 1508
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 7 cut(s) 105, 283, 310, 959, 977, 1064, 1502
PflMI CCANNNNNTGG 1 cut(s) 485
PinAI ACCGGT 1 cut(s) 1302
PkrI GCNGC 2 cut(s) 618, 1249
PscI ACATGT 1 cut(s) 778
PshAI GACNNNNGTC 1 cut(s) 1494
PshBI ATTAAT 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 1266
Psp6I CCWGG 2 cut(s) 980, 1586
PspGI CCWGG 2 cut(s) 980, 1586
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 2 cut(s) 1172, 1220
PspXI VCTCGAGB 1 cut(s) 1265
PstNI CAGNNNCTG 1 cut(s) 1493
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 1 cut(s) 789
SacI GAGCTC 1 cut(s) 1266
SaqAI TTAA 4 cut(s) 246, 264, 423, 1140
SatI GCNGC 2 cut(s) 617, 1248
Sau96I GGNCC 2 cut(s) 1172, 1220
ScrFI CCNGG 2 cut(s) 982, 1588
SduI GDGCHC 2 cut(s) 1047, 1266
SfaNI GCATC 4 cut(s) 47, 503, 727, 1090
Sfr274I CTCGAG 1 cut(s) 1265
SlaI CTCGAG 1 cut(s) 1265
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 3 cut(s) 288, 728, 1265
SmoI CTYRAG 3 cut(s) 288, 728, 1265
SphI GCATGC 1 cut(s) 1392
Sse9I AATT 7 cut(s) 218, 524, 810, 922, 1028, 1078, 1254
SsiI CCGC 2 cut(s) 616, 936
SspI AATATT 1 cut(s) 710
SspMI CTAG 1 cut(s) 48
SstI GAGCTC 1 cut(s) 1266
StyD4I CCNGG 2 cut(s) 980, 1586
TaaI ACNGT 3 cut(s) 115, 187, 1363
TaiI ACGT 3 cut(s) 862, 1542, 1599
TaqI TCGA 3 cut(s) 222, 546, 1266
TasI AATT 7 cut(s) 218, 524, 810, 922, 1028, 1078, 1254
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 619
TfiI GAWTC 7 cut(s) 105, 283, 310, 959, 977, 1064, 1502
Tru1I TTAA 4 cut(s) 246, 264, 423, 1140
Tru9I TTAA 4 cut(s) 246, 264, 423, 1140
TscAI CASTG 1 cut(s) 1498
TseFI GTSAC 1 cut(s) 1483
TseI GCWGC 1 cut(s) 1247
Tsp45I GTSAC 1 cut(s) 1483
TspDTI ATGAA 6 cut(s) 231, 296, 536, 791, 1247, 1460
TspGWI ACGGA 5 cut(s) 89, 200, 473, 906, 1043
TspRI CASTG 1 cut(s) 1498
Van91I CCANNNNNTGG 1 cut(s) 485
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 3 cut(s) 218, 810, 1028
XceI RCATGY 3 cut(s) 607, 782, 1392
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 1265
XmnI GAANNNNTTC 1 cut(s) 58
XspI CTAG 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.