Rh4CG136400

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006733
Physical Location & Seq
Forward (+)
26975556 .. 26986172
10617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG136400.1

Sequence Viewer

Length: 1260 bp
ATGGCCTTGTTTCGCAGGCTGCTTCCTCCTCTCTGCTCTTCCATCAACAGGACGAGCTTTTACTCATCTGGAGCTCTACAAGGTTTTCGGAGTTATGGAGCCATTGCTACACCCGAGCCAACCGTGTTGAAATTTGAGAATGGAGAGGCTGCTGATTCTGTGACTGCTGGTGATGAGATAGCAAAGATTCGGCGTGAATTTGATGCCGCAAAGCAGAGTTTCCTCAGGATTCCACAGGCACTTAGAGAAGTGCCGAAGATGAATCCGGAAGGGATATATGTAAATAAAAACCTGAGGTTGGACAGTATTCAAGTTTATGGATTTGACTATGACTACACGCTGGTGCATTACTCGGCAAATTTACAGAACTTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGTTCCGGTATCCTGAGGTTTGCATGGAATTTAAGTACGATCCTACTTTTCCCATTAGAGGACTGTGCTATGACAAATTGAAAGGGTGTCTCTTGAAGCTGGACTTTTTTGGGTCAATTGAGCCAGATGGATGCTACTATGGTCGTCGAAAGCTTAGTAGGAAGGAAATAGATGAGATATATGGCACAAGGCGTATCGGTCGTGATCAAGCAGGAAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGCGAGGCATGCCTGATTGCAGACATTGTACAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGTTACATCTATCAAGATGTAAATCGTGCGATTCAGCATGTTCACCGTACTGGACTTGTTCATAGAGGAATTCTTTCTGATCCCCGTAAATATCTTGTTAAAAATGGCCAGCTGTTACACTTTCTGAAGATGCTCAAGGAAAAAGGAAAGAAACTTTTCTTGCTGACTAACTCTCCATATTACTTTGTGGATGGAGGAATGCGTTTTTTGTTGGAGGATTCTTTGGGTTGTAATGACTCTTGGAGGGAACTTTTTGACGTTGTGATTGCCAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTGTTATGATGCAGAGAAGGATAATTTAGCTTTCATGAAGGTGGATGCGTTTCTTCCTAATAAAATTTATTACCACGGATGCCTGAAATCATTCCTTCAAATTACGAAGTGGAAGGGACCAGAGGTGATATACTTTGGTGATCACCTCTTTAGCGACCTAAGGGGACCATCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAATCTAATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

419

Amino Acids

48.36

Weight (kDa)

8.15

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 92 - 417 5.8e-122 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 1 cut(s) 207
AclWI GGATC 2 cut(s) 440, 794
AcoI YGGCCR 1 cut(s) 826
AcsI RAATTY 7 cut(s) 131, 197, 358, 434, 710, 789, 1098
AcuI CTGAAG 1 cut(s) 866
AfaI GTAC 3 cut(s) 443, 684, 769
AfiI CCNNNNNNNGG 3 cut(s) 48, 298, 464
AgsI TTSAA 5 cut(s) 130, 311, 487, 502, 1133
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 8 cut(s) 57, 74, 505, 559, 706, 832, 1064, 1211
AluI AGCT 8 cut(s) 57, 74, 505, 559, 706, 832, 1064, 1211
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 440, 794
Ama87I CYCGRG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 2 cut(s) 3, 826
ApeKI GCWGC 3 cut(s) 19, 149, 1226
ApoI RAATTY 7 cut(s) 131, 197, 358, 434, 710, 789, 1098
Asp700I GAANNNNTTC 3 cut(s) 793, 875, 1124
AspLEI GCGC 1 cut(s) 1221
AspS9I GGNCC 2 cut(s) 1151, 1199
AsuHPI GGTGA 5 cut(s) 182, 755, 1169, 1171, 1184
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 1151, 1199
AxyI CCTNAGG 4 cut(s) 224, 293, 420, 1193
BalI TGGCCA 1 cut(s) 828
BanII GRGCYC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 3 cut(s) 6, 136, 1213
BccI CCATC 5 cut(s) 50, 527, 631, 905, 1210
BciVI GTATCC 1 cut(s) 426
BclI TGATCA 2 cut(s) 610, 1174
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 1244
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 4 cut(s) 20, 150, 207, 1227
BlpI GCTNAGC 1 cut(s) 702
BlsI GCNGC 4 cut(s) 21, 151, 208, 1228
Bme18I GGWCC 2 cut(s) 1151, 1199
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 1151, 1199
BmiI GGNNCC 3 cut(s) 100, 1152, 1200
BmsI GCATC 9 cut(s) 193, 527, 688, 706, 840, 1033, 1033, 1069, 1103
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 387
Bpu1102I GCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 839
BsaBI GATNNNNATC 1 cut(s) 741
BsaJI CCNNGG 1 cut(s) 1108
BsaWI WCCGGW 2 cut(s) 265, 411
BsaXI ACNNNNNCTCC 2 cut(s) 877, 907
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 298, 464
Bse1I ACTGG 1 cut(s) 775
Bse21I CCTNAGG 4 cut(s) 224, 293, 420, 1193
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 265
BseDI CCNNGG 1 cut(s) 1108
BseGI GGATG 6 cut(s) 542, 703, 916, 1024, 1084, 1118
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 3 cut(s) 48, 298, 464
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 4 cut(s) 238, 284, 411, 1011
BseNI ACTGG 1 cut(s) 775
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 3 cut(s) 6, 136, 1213
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 2 cut(s) 5, 828
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 2 cut(s) 266, 412
BslFI GGGAC 2 cut(s) 1164, 1212
BslI CCNNNNNNNGG 3 cut(s) 48, 298, 464
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 1164, 1212
BsmI GAATGC 1 cut(s) 924
BsnI GGCC 2 cut(s) 5, 828
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 76
Bsp13I TCCGGA 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 5 cut(s) 379, 445, 610, 799, 1174
Bsp1720I GCTNAGC 1 cut(s) 702
BspACI CCGC 1 cut(s) 207
BspANI GGCC 2 cut(s) 5, 828
BspCNI CTCAG 4 cut(s) 237, 285, 412, 1012
BspEI TCCGGA 1 cut(s) 265
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 3 cut(s) 100, 1152, 1200
BspPI GGATC 2 cut(s) 440, 794
BspQI GCTCTTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 102
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 1 cut(s) 775
BssECI CCNNGG 1 cut(s) 1108
BssMI GATC 5 cut(s) 379, 445, 610, 799, 1174
Bst4CI ACNGT 4 cut(s) 124, 305, 471, 767
Bst6I CTCTTC 1 cut(s) 43
BstAUI TGTACA 1 cut(s) 682
BstC8I GCNNGC 3 cut(s) 17, 664, 830
BstDEI CTNAG 9 cut(s) 224, 242, 293, 387, 420, 560, 702, 1020, 1193
BstDSI CCRYGG 1 cut(s) 1108
BstF5I GGATG 6 cut(s) 542, 703, 916, 1024, 1084, 1118
BstHHI GCGC 1 cut(s) 1221
BstKTI GATC 5 cut(s) 382, 448, 613, 802, 1177
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 379, 445, 610, 799, 1174
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 1 cut(s) 663
BstNSI RCATGY 2 cut(s) 666, 761
BstV1I GCAGC 3 cut(s) 6, 136, 1213
Bsu36I CCTNAGG 4 cut(s) 224, 293, 420, 1193
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 2 cut(s) 5, 828
BtgI CCRYGG 1 cut(s) 1108
BtsCI GGATG 6 cut(s) 542, 703, 916, 1024, 1084, 1118
BtsI GCAGTG 1 cut(s) 1221
BtsIMutI CAGTG 1 cut(s) 1221
Cac8I GCNNGC 3 cut(s) 17, 664, 830
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 1 cut(s) 1221
Cfr13I GGNCC 2 cut(s) 1151, 1199
Csp6I GTAC 3 cut(s) 442, 683, 768
CviAII CATG 5 cut(s) 430, 663, 758, 1069, 1238
CviQI GTAC 3 cut(s) 442, 683, 768
DdeI CTNAG 9 cut(s) 224, 242, 293, 387, 420, 560, 702, 1020, 1193
DpnI GATC 5 cut(s) 381, 447, 612, 801, 1176
DpnII GATC 5 cut(s) 379, 445, 610, 799, 1174
EaeI YGGCCR 1 cut(s) 826
Eam1104I CTCTTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 1151, 1199
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 866
Eco81I CCTNAGG 4 cut(s) 224, 293, 420, 1193
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 789
EcoT38I GRGCYC 1 cut(s) 76
FaeI CATG 5 cut(s) 433, 666, 761, 1072, 1241
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 2 cut(s) 1164, 1212
FatI CATG 5 cut(s) 429, 662, 757, 1068, 1237
FauNDI CATATG 1 cut(s) 396
FbaI TGATCA 2 cut(s) 610, 1174
Fnu4HI GCNGC 4 cut(s) 20, 150, 207, 1227
FokI GGATG 6 cut(s) 549, 710, 923, 1011, 1091, 1125
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 4 cut(s) 20, 150, 207, 1227
FspBI CTAG 1 cut(s) 1244
GlaI GCGC 1 cut(s) 1220
GluI GCNGC 4 cut(s) 20, 150, 207, 1227
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 2 cut(s) 5, 828
HapII CCGG 2 cut(s) 266, 412
HhaI GCGC 1 cut(s) 1221
Hin1II CATG 5 cut(s) 433, 666, 761, 1072, 1241
Hin6I GCGC 1 cut(s) 1219
HinP1I GCGC 1 cut(s) 1219
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 8 cut(s) 155, 187, 229, 262, 751, 938, 956, 1247
HpaII CCGG 2 cut(s) 266, 412
HphI GGTGA 5 cut(s) 182, 755, 1169, 1171, 1184
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 4 cut(s) 90, 799, 846, 1021
Hpy188III TCNNGA 8 cut(s) 69, 226, 266, 419, 499, 608, 734, 1069
Hpy8I GTNNAC 1 cut(s) 763
Hpy99I CGWCG 1 cut(s) 555
HpyAV CCTTC 6 cut(s) 263, 562, 1045, 1066, 1139, 1141
HpyCH4III ACNGT 4 cut(s) 124, 305, 471, 767
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 4 cut(s) 346, 429, 674, 1046
HpyF10VI GCNNNNNNNGC 1 cut(s) 663
HpyF3I CTNAG 9 cut(s) 224, 242, 293, 387, 420, 560, 702, 1020, 1193
HpySE526I ACGT 1 cut(s) 978
Hsp92II CATG 5 cut(s) 433, 666, 761, 1072, 1241
HspAI GCGC 1 cut(s) 1219
Kpn2I TCCGGA 1 cut(s) 265
Ksp22I TGATCA 2 cut(s) 610, 1174
Kzo9I GATC 5 cut(s) 379, 445, 610, 799, 1174
LguI GCTCTTC 1 cut(s) 43
LmnI GCTCC 3 cut(s) 71, 98, 391
Lsp1109I GCAGC 3 cut(s) 6, 136, 1213
LweI GCATC 9 cut(s) 193, 527, 688, 706, 840, 1033, 1033, 1069, 1103
MaeI CTAG 1 cut(s) 1244
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 3 cut(s) 160, 722, 834
MalI GATC 5 cut(s) 381, 447, 612, 801, 1176
MboI GATC 5 cut(s) 379, 445, 610, 799, 1174
MboII GAAGA 5 cut(s) 30, 268, 637, 859, 1079
MfeI CAATTG 1 cut(s) 522
MhlI GDGCHC 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 828
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 1 cut(s) 950
MmeI TCCRAC 2 cut(s) 279, 912
Mox20I TGGCCA 1 cut(s) 828
MroI TCCGGA 1 cut(s) 265
MroXI GAANNNNTTC 3 cut(s) 793, 875, 1124
MscI TGGCCA 1 cut(s) 828
MseI TTAA 2 cut(s) 438, 819
MslI CAYNNNNRTG 2 cut(s) 341, 1073
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 832
MspI CCGG 2 cut(s) 266, 412
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 924
MwoI GCNNNNNNNGC 1 cut(s) 663
NdeI CATATG 1 cut(s) 396
NdeII GATC 5 cut(s) 379, 445, 610, 799, 1174
NlaIII CATG 5 cut(s) 433, 666, 761, 1072, 1241
NlaIV GGNNCC 3 cut(s) 100, 1152, 1200
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 2 cut(s) 666, 761
OliI CACNNNNGTG 1 cut(s) 341
PaeI GCATGC 1 cut(s) 666
PagI TCATGA 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 43
PctI GAATGC 1 cut(s) 924
PdmI GAANNNNTTC 3 cut(s) 793, 875, 1124
PfeI GAWTC 7 cut(s) 155, 187, 229, 262, 751, 938, 1247
PkrI GCNGC 4 cut(s) 21, 151, 208, 1228
PleI GAGTC 1 cut(s) 950
PpsI GAGTC 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 76
PspN4I GGNNCC 3 cut(s) 100, 1152, 1200
PspPI GGNCC 2 cut(s) 1151, 1199
PvuII CAGCTG 1 cut(s) 832
RsaI GTAC 3 cut(s) 443, 684, 769
RsaNI GTAC 3 cut(s) 442, 683, 768
RseI CAYNNNNRTG 2 cut(s) 341, 1073
SacI GAGCTC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 43
SaqAI TTAA 2 cut(s) 438, 819
SatI GCNGC 4 cut(s) 20, 150, 207, 1227
Sau3AI GATC 5 cut(s) 379, 445, 610, 799, 1174
Sau96I GGNCC 2 cut(s) 1151, 1199
SchI GAGTC 1 cut(s) 950
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 9 cut(s) 193, 527, 688, 706, 840, 1033, 1033, 1069, 1103
SinI GGWCC 2 cut(s) 1151, 1199
SmiMI CAYNNNNRTG 2 cut(s) 341, 1073
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SphI GCATGC 1 cut(s) 666
SsiI CCGC 1 cut(s) 207
SspI AATATT 1 cut(s) 689
SspMI CTAG 1 cut(s) 1244
SstI GAGCTC 1 cut(s) 76
TaaI ACNGT 4 cut(s) 124, 305, 471, 767
TaiI ACGT 1 cut(s) 981
TaqI TCGA 1 cut(s) 553
TaqII GACCGA 1 cut(s) 593
TatI WGTACW 1 cut(s) 682
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 7 cut(s) 155, 187, 229, 262, 751, 938, 1247
Tru1I TTAA 2 cut(s) 438, 819
Tru9I TTAA 2 cut(s) 438, 819
TscAI CASTG 1 cut(s) 1228
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 3 cut(s) 19, 149, 1226
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 5 cut(s) 275, 770, 1057, 1085, 1254
TspGWI ACGGA 2 cut(s) 1022, 1125
TspRI CASTG 1 cut(s) 1228
VpaK11BI GGWCC 2 cut(s) 1151, 1199
XapI RAATTY 7 cut(s) 131, 197, 358, 434, 710, 789, 1098
XceI RCATGY 2 cut(s) 666, 761
XmnI GAANNNNTTC 3 cut(s) 793, 875, 1124
XspI CTAG 1 cut(s) 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.