Rh4DG122800

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006733
Physical Location & Seq
Forward (+)
19735643 .. 19745699
10057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG122800.1

Sequence Viewer

Length: 1641 bp
ATGGCCTTGTTTCGCAGGCTGCTTCCTCCTCTCTGCTCTTCCATCAACAGGACGAGCTTTTGCTCATCTGGAGCTCTACAAGGTTTTCGGAGTTATGGAGCCATTGATACACCCGAGCCAACCGTGTTGAAGAAATTTGAGAATGGAGAGACGGCTGATTCTGTGACTTCTGATGATGAGATAGCAAAGATTCGGCGTGAATTTGATGCCGCAAAGCAGAGTTTCCTCAAGATTCCACAGGCACTTCAAGAATTGCCGAAGATGAATCCGGAAGGGATATATGTAAATAAAAACCTGAGGTTGGACAGTATTCAAGTTTATGGATTTGACTATGACTACACGCTGGTGCATTACTCGGCAGATTTACAGAACTTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGTTCCGGTATCCTCATGTTTGCATGGAATTTAAGTACGATCCTACTTTTCCCATTAGAGGACTGTGCTATGACAAATTGAAAGGGTGTCTCTTGAAGCTGGACTTTTTTGGGTCAATTGAGCCAGATGGATGCTACTATGGTCGTCGAAAGCTTAGTAGGAAGGAAATAGATGAGATATATGGCACAAGGCGTATCGGTCGTGATCAAGCAGGAAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGTGAGGCATGCCTGATTGCAGACATCGTACAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTAAATCGTGCGATTCAGCATGTTCACCATACAGGCCTTGTTCATAGAGGAATTCTTTCTGATCCCCGTAAATATCTTGTTAAAAATGACCAGCTGTTACATTTTCTGAAGATGCTCAGGGAAAAAGGAAAGAAACTTTTCTTGCTGACTAACTCTCCATATTACTTTGTGGATAGAGGAATGCGTTTTTTGTTGGAGGATTATTTGGGTTGTAATGACTCTTGGAGGGAACTTTTTGACGTTGTGATTGCCAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTGTTATGATGCAGAGAAGGATAATTTAGCTTTCATGAAGGTGGATGCGTTTCTTCCTAATAAAATTTATTACCACGGATGCCTGAAATCATTCCTTCAAATTACTAAGTGGAAGGGACCAGAGGTGATATACTTTGGTGATCACCTCTTTAGCGACCTAAGGGGACCATCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAAGATGAAATACGTATACAAAATGAGGATGCTTACCGCGTGGAGCAGGCAAAATTTCATATATTCCAAGAATTGCTTGGTAGACTGCATGCAACGGTAGCTAATAGTCAGAAAAGTGAGGCCAAGTTACTGTTGGAGGAGCTAAATGAGTGCAGGCGTAAGGCAAGCAACAAGATGAAGAGAATGTTCAATAGCTCTTTCGGAGCCACTTTCCTTACGGATAAAGGTCAAGAATCTGCTTTCTCGTATCATATTCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTTCATGCACCCTACGATATTAAGATTATGCCACATCATGTGAAGGTTTCATCCATCTTGTTCAAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

546

Amino Acids

63.48

Weight (kDa)

7.26

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 93 - 537 4.9e-149 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1264, 1330
AccII CGCG 1 cut(s) 1287
AccIII TCCGGA 1 cut(s) 268
AciI CCGC 2 cut(s) 210, 1285
AclWI GGATC 2 cut(s) 443, 797
AcsI RAATTY 8 cut(s) 134, 200, 437, 713, 792, 1101, 1301, 1540
AcuI CTGAAG 2 cut(s) 869, 1581
AfaI GTAC 2 cut(s) 446, 687
AfiI CCNNNNNNNGG 3 cut(s) 48, 301, 467
AgsI TTSAA 8 cut(s) 130, 248, 314, 490, 505, 1136, 1438, 1633
AleI CACNNNNGTG 1 cut(s) 344
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 2 cut(s) 143, 503
AlwI GGATC 2 cut(s) 443, 797
Ama87I CYCGRG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 3 cut(s) 3, 775, 1368
ApeKI GCWGC 2 cut(s) 19, 1229
ApoI RAATTY 8 cut(s) 134, 200, 437, 713, 792, 1101, 1301, 1540
Asp700I GAANNNNTTC 3 cut(s) 796, 878, 1127
AspLEI GCGC 1 cut(s) 1224
AspS9I GGNCC 2 cut(s) 1154, 1202
AsuHPI GGTGA 4 cut(s) 758, 1172, 1174, 1187
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 1154, 1202
AxyI CCTNAGG 2 cut(s) 296, 1196
BanII GRGCYC 1 cut(s) 76
BarI GAAGNNNNNNTAC 2 cut(s) 1242, 1274
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 2 cut(s) 6, 1216
BccI CCATC 5 cut(s) 50, 530, 634, 1213, 1631
BceAI ACGGC 1 cut(s) 168
BciVI GTATCC 1 cut(s) 429
BclI TGATCA 2 cut(s) 613, 1177
BcoDI GTCTC 2 cut(s) 143, 503
BfaI CTAG 1 cut(s) 1247
BfuI GTATCC 1 cut(s) 429
BisI GCNGC 3 cut(s) 20, 210, 1230
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 3 cut(s) 21, 211, 1231
Bme18I GGWCC 2 cut(s) 1154, 1202
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 1154, 1202
BmiI GGNNCC 4 cut(s) 100, 1155, 1203, 1453
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 2 cut(s) 390, 857
Bpu1102I GCTNAGC 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 212
BsaAI YACGTR 1 cut(s) 1262
BsaBI GATNNNNATC 1 cut(s) 744
BsaJI CCNNGG 1 cut(s) 1111
BsaWI WCCGGW 2 cut(s) 268, 414
BsaXI ACNNNNNCTCC 2 cut(s) 880, 910
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 301, 467
Bse1I ACTGG 1 cut(s) 1527
Bse21I CCTNAGG 2 cut(s) 296, 1196
Bse8I GATNNNNATC 1 cut(s) 744
BseAI TCCGGA 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 1111
BseGI GGATG 7 cut(s) 545, 706, 1027, 1087, 1121, 1282, 1619
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 3 cut(s) 48, 301, 467
BseMII CTCAG 3 cut(s) 287, 871, 1014
BseNI ACTGG 1 cut(s) 1527
BseRI GAGGAG 2 cut(s) 18, 1400
BseXI GCAGC 2 cut(s) 6, 1216
BsgI GTGCAG 1 cut(s) 1420
Bsh1236I CGCG 1 cut(s) 1287
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 5, 777, 1370
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 2 cut(s) 269, 415
BslFI GGGAC 2 cut(s) 1167, 1215
BslI CCNNNNNNNGG 3 cut(s) 48, 301, 467
BsmAI GTCTC 2 cut(s) 143, 503
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 2 cut(s) 1167, 1215
BsmI GAATGC 1 cut(s) 927
BsnI GGCC 3 cut(s) 5, 777, 1370
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 76
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 5 cut(s) 382, 448, 613, 802, 1177
Bsp1720I GCTNAGC 1 cut(s) 705
BspACI CCGC 2 cut(s) 210, 1285
BspANI GGCC 3 cut(s) 5, 777, 1370
BspCNI CTCAG 3 cut(s) 288, 870, 1015
BspEI TCCGGA 1 cut(s) 268
BspFNI CGCG 1 cut(s) 1287
BspHI TCATGA 1 cut(s) 1071
BspLI GGNNCC 4 cut(s) 100, 1155, 1203, 1453
BspPI GGATC 2 cut(s) 443, 797
BspQI GCTCTTC 1 cut(s) 43
BsrI ACTGG 1 cut(s) 1527
BssECI CCNNGG 1 cut(s) 1111
BssMI GATC 5 cut(s) 382, 448, 613, 802, 1177
BssNAI GTATAC 1 cut(s) 1265
Bst1107I GTATAC 1 cut(s) 1265
Bst4CI ACNGT 5 cut(s) 124, 308, 474, 1345, 1380
Bst6I CTCTTC 2 cut(s) 43, 1421
BstBAI YACGTR 1 cut(s) 1262
BstC8I GCNNGC 7 cut(s) 17, 667, 724, 1296, 1338, 1403, 1414
BstDEI CTNAG 8 cut(s) 296, 390, 563, 705, 857, 1023, 1143, 1196
BstDSI CCRYGG 1 cut(s) 1111
BstF5I GGATG 7 cut(s) 545, 706, 1027, 1087, 1121, 1282, 1619
BstFNI CGCG 1 cut(s) 1287
BstHHI GCGC 1 cut(s) 1224
BstKTI GATC 5 cut(s) 385, 451, 616, 805, 1180
BstMAI GTCTC 2 cut(s) 143, 503
BstMBI GATC 5 cut(s) 382, 448, 613, 802, 1177
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 1 cut(s) 1346
BstNSI RCATGY 3 cut(s) 669, 764, 1340
BstSNI TACGTA 1 cut(s) 1262
BstUI CGCG 1 cut(s) 1287
BstV1I GCAGC 2 cut(s) 6, 1216
BstZ17I GTATAC 1 cut(s) 1265
Bsu36I CCTNAGG 2 cut(s) 296, 1196
BsuI GTATCC 1 cut(s) 429
BsuRI GGCC 3 cut(s) 5, 777, 1370
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 7 cut(s) 545, 706, 1027, 1087, 1121, 1282, 1619
BtsI GCAGTG 1 cut(s) 1224
BtsIMutI CAGTG 1 cut(s) 1224
Cac8I GCNNGC 7 cut(s) 17, 667, 724, 1296, 1338, 1403, 1414
CciI TCATGA 1 cut(s) 1071
CfoI GCGC 1 cut(s) 1224
Cfr13I GGNCC 2 cut(s) 1154, 1202
Csp6I GTAC 2 cut(s) 445, 686
CviQI GTAC 2 cut(s) 445, 686
DdeI CTNAG 8 cut(s) 296, 390, 563, 705, 857, 1023, 1143, 1196
DpnI GATC 5 cut(s) 384, 450, 615, 804, 1179
DpnII GATC 5 cut(s) 382, 448, 613, 802, 1177
Eam1104I CTCTTC 2 cut(s) 43, 1421
EarI CTCTTC 2 cut(s) 43, 1421
Ecl136II GAGCTC 1 cut(s) 74
Eco105I TACGTA 1 cut(s) 1262
Eco147I AGGCCT 1 cut(s) 777
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 1154, 1202
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 2 cut(s) 869, 1581
Eco81I CCTNAGG 2 cut(s) 296, 1196
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 143
FalI AAGNNNNNCTT 6 cut(s) 497, 529, 1308, 1340, 1554, 1586
FaqI GGGAC 2 cut(s) 1167, 1215
FauNDI CATATG 1 cut(s) 399
FbaI TGATCA 2 cut(s) 613, 1177
FblI GTMKAC 2 cut(s) 1264, 1330
Fnu4HI GCNGC 3 cut(s) 20, 210, 1230
FokI GGATG 7 cut(s) 552, 713, 1014, 1094, 1128, 1289, 1606
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 3 cut(s) 20, 210, 1230
FspBI CTAG 1 cut(s) 1247
GlaI GCGC 1 cut(s) 1223
GluI GCNGC 3 cut(s) 20, 210, 1230
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 3 cut(s) 5, 777, 1370
HapII CCGG 2 cut(s) 269, 415
HhaI GCGC 1 cut(s) 1224
Hin6I GCGC 1 cut(s) 1222
HinP1I GCGC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 7 cut(s) 158, 190, 232, 265, 754, 959, 1481
HpaII CCGG 2 cut(s) 269, 415
HphI GGTGA 4 cut(s) 758, 1172, 1174, 1187
Hpy166II GTNNAC 3 cut(s) 766, 1265, 1331
Hpy188I TCNGA 7 cut(s) 90, 172, 802, 849, 1024, 1359, 1451
Hpy8I GTNNAC 3 cut(s) 766, 1265, 1331
Hpy99I CGWCG 1 cut(s) 558
HpyAV CCTTC 7 cut(s) 266, 565, 1048, 1069, 1142, 1144, 1606
HpyCH4III ACNGT 5 cut(s) 124, 308, 474, 1345, 1380
HpyCH4IV ACGT 2 cut(s) 981, 1261
HpyCH4V TGCA 9 cut(s) 349, 432, 677, 1049, 1336, 1340, 1401, 1514, 1577
HpyF10VI GCNNNNNNNGC 1 cut(s) 1346
HpyF3I CTNAG 8 cut(s) 296, 390, 563, 705, 857, 1023, 1143, 1196
HpySE526I ACGT 2 cut(s) 981, 1261
HspAI GCGC 1 cut(s) 1222
Kpn2I TCCGGA 1 cut(s) 268
Ksp22I TGATCA 2 cut(s) 613, 1177
Kzo9I GATC 5 cut(s) 382, 448, 613, 802, 1177
LguI GCTCTTC 1 cut(s) 43
LmnI GCTCC 6 cut(s) 71, 98, 394, 1291, 1387, 1451
Lsp1109I GCAGC 2 cut(s) 6, 1216
MaeI CTAG 1 cut(s) 1247
MaeII ACGT 2 cut(s) 981, 1261
MaeIII GTNAC 3 cut(s) 163, 837, 1374
MalI GATC 5 cut(s) 384, 450, 615, 804, 1179
MboI GATC 5 cut(s) 382, 448, 613, 802, 1177
MboII GAAGA 8 cut(s) 30, 142, 271, 640, 862, 1082, 1262, 1438
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 76
MlyI GAGTC 1 cut(s) 953
MmeI TCCRAC 3 cut(s) 282, 915, 1362
MroI TCCGGA 1 cut(s) 268
MroXI GAANNNNTTC 3 cut(s) 796, 878, 1127
MseI TTAA 4 cut(s) 441, 822, 1590, 1639
MslI CAYNNNNRTG 3 cut(s) 344, 1076, 1509
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 2 cut(s) 269, 415
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 927
MvnI CGCG 1 cut(s) 1287
MwoI GCNNNNNNNGC 1 cut(s) 1346
NdeI CATATG 1 cut(s) 399
NdeII GATC 5 cut(s) 382, 448, 613, 802, 1177
NlaIV GGNNCC 4 cut(s) 100, 1155, 1203, 1453
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 3 cut(s) 669, 764, 1340
OliI CACNNNNGTG 1 cut(s) 344
PaeI GCATGC 2 cut(s) 669, 1340
PagI TCATGA 1 cut(s) 1071
PceI AGGCCT 1 cut(s) 777
PciSI GCTCTTC 1 cut(s) 43
PctI GAATGC 1 cut(s) 927
PdmI GAANNNNTTC 3 cut(s) 796, 878, 1127
PfeI GAWTC 6 cut(s) 158, 190, 232, 265, 754, 1481
PkrI GCNGC 3 cut(s) 21, 211, 1231
PleI GAGTC 1 cut(s) 953
PpsI GAGTC 1 cut(s) 953
Ppu21I YACGTR 1 cut(s) 1262
Psp124BI GAGCTC 1 cut(s) 76
PspN4I GGNNCC 4 cut(s) 100, 1155, 1203, 1453
PspPI GGNCC 2 cut(s) 1154, 1202
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 2 cut(s) 446, 687
RsaNI GTAC 2 cut(s) 445, 686
RseI CAYNNNNRTG 3 cut(s) 344, 1076, 1509
SacI GAGCTC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 43
SaqAI TTAA 4 cut(s) 441, 822, 1590, 1639
SatI GCNGC 3 cut(s) 20, 210, 1230
Sau3AI GATC 5 cut(s) 382, 448, 613, 802, 1177
Sau96I GGNCC 2 cut(s) 1154, 1202
SchI GAGTC 1 cut(s) 953
SduI GDGCHC 1 cut(s) 76
SinI GGWCC 2 cut(s) 1154, 1202
SmiMI CAYNNNNRTG 3 cut(s) 344, 1076, 1509
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SnaBI TACGTA 1 cut(s) 1262
SphI GCATGC 2 cut(s) 669, 1340
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 2 cut(s) 210, 1285
SspI AATATT 1 cut(s) 692
SspMI CTAG 1 cut(s) 1247
SstI GAGCTC 1 cut(s) 76
StuI AGGCCT 1 cut(s) 777
TaaI ACNGT 5 cut(s) 124, 308, 474, 1345, 1380
TaiI ACGT 2 cut(s) 984, 1264
TaqI TCGA 1 cut(s) 556
TaqII GACCGA 1 cut(s) 596
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 6 cut(s) 158, 190, 232, 265, 754, 1481
Tru1I TTAA 4 cut(s) 441, 822, 1590, 1639
Tru9I TTAA 4 cut(s) 441, 822, 1590, 1639
TscAI CASTG 1 cut(s) 1231
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 2 cut(s) 19, 1229
Tsp45I GTSAC 1 cut(s) 163
TspGWI ACGGA 3 cut(s) 1025, 1128, 1481
TspRI CASTG 1 cut(s) 1231
VpaK11BI GGWCC 2 cut(s) 1154, 1202
XapI RAATTY 8 cut(s) 134, 200, 437, 713, 792, 1101, 1301, 1540
XceI RCATGY 3 cut(s) 669, 764, 1340
XcmI CCANNNNNNNNNTGG 3 cut(s) 1322, 1378, 1563
XmiI GTMKAC 2 cut(s) 1264, 1330
XmnI GAANNNNTTC 3 cut(s) 796, 878, 1127
XspI CTAG 1 cut(s) 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.