FvH4_4g08620

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006733
Physical Location & Seq
Reverse (-)
8963740 .. 8974179
10440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08620.t6

Sequence Viewer

Length: 1389 bp
ATGCCTAAGATGAATCCGGAAGGGATATATGTGAATAAGAACCTGAGATTGGACAGTATTCAAGTTTATGGATTCGACTATGACTACACGCTGGCACATTACTCGTCAGAATTACAGAACTTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGTATCACTATCCTGAGGTTTGCATGGAATTCAAGTATGATCCAAGTTTTCCCATTAGAGGACTAAGCTATGACAAGTCGCGAGGGTGTCTCTTGAAGCTGGACTTCTTTGGGTCAATCGAGCCAGATGGATGCTACTATGGTCGTCGAAAGCTTAGAAGGAAGGAAATAGATGAGATGTATGGCACAAGGCGTATCGGTCGTGATCAAGCAGCTAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGCGAGGCATGCCTCATTGCAGACATCGTGCAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTCAATCGTGCAATTCAGCATGTTCACCGTACTGGCCTTGTTCATAGAGGAATTCTTTCTGATCCCCATAGATATCTTGTTAAAAATGGCCAGCTGTTACACTTTCTGAAGATGCTCAGGGAAAAAGGAAAAAAACTATTCTTGCTGACTAACTCTCCATACTACTTTGTGGATGGAGGAATGCGTTTTTTGTTGGAGGATTCTTTGGGTCTTAATGACTCTTGGAGGGAGCTTTTTGATGTTGTGATTGCCAAAGCCAATAAGCCAGATTTTTACACATCTGAGCATCCATTTCGTTGTTATGATGCAGAAAAAGATACTTTAGCTTTCATGAAGGTGGATGCGTTTCTTCCTAATAATGTTTATTACCATGGATGCCTGAAATCATTTCTTCAAATTACCAAGTGGAAGGGACCAGAGGTGATATACTTTGGAGATCACCTCTTTAGCGACCTAAGGGGACCGTCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAAGATGAAATACTTGTACAGAATCAGGATGCTTACCGGGTGGAGCAGGCTAAATTTCATATATTCCAAGAATTGCTTGGTAGACTGCATTCAACGGTATCTACTAGTCAGAAAAGTGAGGCCAATGTACTGTTGGAGGAGCTAAATGAGTGCAGACGTAAGGCAAGCCACAAGATGAAGGCAATGTTTAATACATCTTTCGGAGCCACCTTCCTTACAGATAAAGGTCAAGAATCTGCTTTTGCGTATCATATCCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACTTGAAGCATGGCTTCATGCACCCTTCGATGTTAAGATTATGCCACATCATGTGAAGGTTTCATCCAGCTTGTTCAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

463

Amino Acids

53.86

Weight (kDa)

6.58

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 9 - 453 2.9e-148 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1078
AccII CGCG 1 cut(s) 238
AccIII TCCGGA 1 cut(s) 16
AclWI GGATC 2 cut(s) 191, 545
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 5 cut(s) 185, 461, 540, 1049, 1288
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 3 cut(s) 520, 1014, 1125
AfiI CCNNNNNNNGG 2 cut(s) 49, 215
AgsI TTSAA 7 cut(s) 62, 190, 253, 884, 1089, 1310, 1381
AhlI ACTAGT 1 cut(s) 1100
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 191, 545
Aor13HI TCCGGA 1 cut(s) 16
AoxI GGCC 3 cut(s) 523, 577, 1116
ApeKI GCWGC 2 cut(s) 368, 977
ApoI RAATTY 5 cut(s) 185, 461, 540, 1049, 1288
Asp700I GAANNNNTTC 1 cut(s) 544
AspLEI GCGC 1 cut(s) 972
AspS9I GGNCC 2 cut(s) 902, 950
AsuC2I CCSGG 1 cut(s) 1034
AsuHPI GGTGA 3 cut(s) 506, 920, 922
AsuNHI GCTAGC 1 cut(s) 371
AvaII GGWCC 2 cut(s) 902, 950
AxyI CCTNAGG 2 cut(s) 171, 944
BalI TGGCCA 1 cut(s) 579
BarI GAAGNNNNNNTAC 2 cut(s) 990, 1022
BbvI GCAGC 2 cut(s) 380, 964
BccI CCATC 4 cut(s) 278, 382, 656, 1260
BcgI CGANNNNNNTGC 4 cut(s) 84, 118, 764, 798
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 1034
BcoDI GTCTC 1 cut(s) 251
BcuI ACTAGT 1 cut(s) 1100
BfaI CTAG 3 cut(s) 372, 995, 1101
BisI GCNGC 2 cut(s) 369, 978
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 2 cut(s) 370, 979
Bme1390I CCNGG 1 cut(s) 1034
Bme18I GGWCC 2 cut(s) 902, 950
BmgT120I GGNCC 2 cut(s) 902, 950
BmiI GGNNCC 3 cut(s) 903, 951, 1201
BmrFI CCNGG 1 cut(s) 1034
BmsI GCATC 9 cut(s) 278, 439, 457, 591, 784, 784, 820, 854, 1015
BmtI GCTAGC 1 cut(s) 375
BplI GAGNNNNNCTC 4 cut(s) 231, 263, 915, 947
Bpu10I CCTNAGC 2 cut(s) 138, 605
Bpu1102I GCTNAGC 1 cut(s) 453
BpuMI CCSGG 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 859
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 215
Bse1I ACTGG 2 cut(s) 526, 1275
Bse21I CCTNAGG 2 cut(s) 171, 944
Bse3DI GCAATG 2 cut(s) 420, 1185
Bse8I GATNNNNATC 1 cut(s) 492
BseAI TCCGGA 1 cut(s) 16
BseDI CCNNGG 1 cut(s) 859
BseGI GGATG 8 cut(s) 293, 454, 667, 775, 835, 869, 1030, 1367
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 2 cut(s) 49, 215
BseMI GCAATG 2 cut(s) 420, 1185
BseMII CTCAG 4 cut(s) 35, 162, 619, 762
BseNI ACTGG 2 cut(s) 526, 1275
BseRI GAGGAG 1 cut(s) 1148
BseXI GCAGC 2 cut(s) 380, 964
BsgI GTGCAG 1 cut(s) 1168
Bsh1236I CGCG 1 cut(s) 238
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 3 cut(s) 525, 579, 1118
BsiEI CGRYCG 1 cut(s) 358
BsiSI CCGG 2 cut(s) 17, 1033
BslFI GGGAC 2 cut(s) 915, 963
BslI CCNNNNNNNGG 2 cut(s) 49, 215
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 2 cut(s) 915, 963
BsmI GAATGC 2 cut(s) 675, 1084
BsnI GGCC 3 cut(s) 525, 579, 1118
Bsp13I TCCGGA 1 cut(s) 16
Bsp1407I TGTACA 1 cut(s) 1012
Bsp143I GATC 5 cut(s) 130, 196, 361, 550, 925
Bsp1720I GCTNAGC 1 cut(s) 453
Bsp19I CCATGG 1 cut(s) 859
Bsp68I TCGCGA 1 cut(s) 238
BspANI GGCC 3 cut(s) 525, 579, 1118
BspCNI CTCAG 4 cut(s) 36, 163, 618, 763
BspEI TCCGGA 1 cut(s) 16
BspFNI CGCG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 3 cut(s) 903, 951, 1201
BspOI GCTAGC 1 cut(s) 375
BspPI GGATC 2 cut(s) 191, 545
BsrDI GCAATG 2 cut(s) 420, 1185
BsrGI TGTACA 1 cut(s) 1012
BsrI ACTGG 2 cut(s) 526, 1275
BssECI CCNNGG 1 cut(s) 859
BssMI GATC 5 cut(s) 130, 196, 361, 550, 925
BssT1I CCWWGG 1 cut(s) 859
Bst4CI ACNGT 5 cut(s) 56, 518, 954, 1093, 1128
BstAUI TGTACA 1 cut(s) 1012
BstC8I GCNNGC 7 cut(s) 93, 373, 415, 472, 581, 1044, 1162
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 8 cut(s) 293, 454, 667, 775, 835, 869, 1030, 1367
BstFNI CGCG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 972
BstKTI GATC 5 cut(s) 133, 199, 364, 553, 928
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 5 cut(s) 130, 196, 361, 550, 925
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNSI RCATGY 2 cut(s) 417, 512
BstSCI CCNGG 1 cut(s) 1032
BstUI CGCG 1 cut(s) 238
BstV1I GCAGC 2 cut(s) 380, 964
Bsu36I CCTNAGG 2 cut(s) 171, 944
BsuRI GGCC 3 cut(s) 525, 579, 1118
BtgI CCRYGG 1 cut(s) 859
BtsCI GGATG 8 cut(s) 293, 454, 667, 775, 835, 869, 1030, 1367
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 1 cut(s) 972
BtuMI TCGCGA 1 cut(s) 238
Cac8I GCNNGC 7 cut(s) 93, 373, 415, 472, 581, 1044, 1162
CciI TCATGA 1 cut(s) 819
CfoI GCGC 1 cut(s) 972
Cfr13I GGNCC 2 cut(s) 902, 950
Csp6I GTAC 3 cut(s) 519, 1013, 1124
CviAII CATG 9 cut(s) 181, 414, 509, 820, 860, 989, 1314, 1322, 1355
CviQI GTAC 3 cut(s) 519, 1013, 1124
DpnI GATC 5 cut(s) 132, 198, 363, 552, 927
DpnII GATC 5 cut(s) 130, 196, 361, 550, 925
EaeI YGGCCR 1 cut(s) 577
Eco130I CCWWGG 1 cut(s) 859
Eco32I GATATC 1 cut(s) 563
Eco47I GGWCC 2 cut(s) 902, 950
Eco57I CTGAAG 1 cut(s) 617
Eco81I CCTNAGG 2 cut(s) 171, 944
EcoRI GAATTC 2 cut(s) 185, 540
EcoRV GATATC 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 9 cut(s) 184, 417, 512, 823, 863, 992, 1317, 1325, 1358
FalI AAGNNNNNCTT 6 cut(s) 245, 277, 1056, 1088, 1302, 1334
FaqI GGGAC 2 cut(s) 915, 963
FatI CATG 9 cut(s) 180, 413, 508, 819, 859, 988, 1313, 1321, 1354
FauNDI CATATG 1 cut(s) 147
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 1 cut(s) 1078
Fnu4HI GCNGC 2 cut(s) 369, 978
FokI GGATG 8 cut(s) 300, 461, 674, 762, 842, 876, 1037, 1354
Fsp4HI GCNGC 2 cut(s) 369, 978
FspBI CTAG 3 cut(s) 372, 995, 1101
GlaI GCGC 1 cut(s) 971
GluI GCNGC 2 cut(s) 369, 978
HaeIII GGCC 3 cut(s) 525, 579, 1118
HapII CCGG 2 cut(s) 17, 1033
HhaI GCGC 1 cut(s) 972
Hin1II CATG 9 cut(s) 184, 417, 512, 823, 863, 992, 1317, 1325, 1358
Hin6I GCGC 1 cut(s) 970
HinP1I GCGC 1 cut(s) 970
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 6 cut(s) 13, 72, 689, 707, 1018, 1229
HpaII CCGG 2 cut(s) 17, 1033
HphI GGTGA 3 cut(s) 506, 920, 922
Hpy166II GTNNAC 2 cut(s) 514, 1079
Hpy188I TCNGA 6 cut(s) 109, 550, 597, 772, 1107, 1199
Hpy8I GTNNAC 2 cut(s) 514, 1079
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 9 cut(s) 14, 309, 313, 817, 892, 1168, 1216, 1339, 1354
HpyCH4III ACNGT 5 cut(s) 56, 518, 954, 1093, 1128
HpyCH4IV ACGT 1 cut(s) 1153
HpyCH4V TGCA 9 cut(s) 180, 425, 436, 500, 797, 1084, 1149, 1262, 1325
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpySE526I ACGT 1 cut(s) 1153
Hsp92II CATG 9 cut(s) 184, 417, 512, 823, 863, 992, 1317, 1325, 1358
HspAI GCGC 1 cut(s) 970
Kpn2I TCCGGA 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 5 cut(s) 130, 196, 361, 550, 925
LmnI GCTCC 5 cut(s) 142, 718, 1039, 1135, 1199
Lsp1109I GCAGC 2 cut(s) 380, 964
LweI GCATC 9 cut(s) 278, 439, 457, 591, 784, 784, 820, 854, 1015
MaeI CTAG 3 cut(s) 372, 995, 1101
MaeII ACGT 1 cut(s) 1153
MaeIII GTNAC 1 cut(s) 585
MalI GATC 5 cut(s) 132, 198, 363, 552, 927
MboI GATC 5 cut(s) 130, 196, 361, 550, 925
MboII GAAGA 5 cut(s) 388, 610, 830, 872, 1010
MlsI TGGCCA 1 cut(s) 579
MluCI AATT 9 cut(s) 110, 185, 461, 501, 540, 885, 1049, 1067, 1288
MluNI TGGCCA 1 cut(s) 579
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 2 cut(s) 663, 1110
Mox20I TGGCCA 1 cut(s) 579
MroI TCCGGA 1 cut(s) 16
MroXI GAANNNNTTC 1 cut(s) 544
MscI TGGCCA 1 cut(s) 579
MseI TTAA 4 cut(s) 570, 702, 1185, 1338
MslI CAYNNNNRTG 2 cut(s) 824, 1257
Msp20I TGGCCA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 583
MspI CCGG 2 cut(s) 17, 1033
MspR9I CCNGG 1 cut(s) 1034
Mva1269I GAATGC 2 cut(s) 675, 1084
MvnI CGCG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 414
NciI CCSGG 1 cut(s) 1034
NcoI CCATGG 1 cut(s) 859
NdeI CATATG 1 cut(s) 147
NdeII GATC 5 cut(s) 130, 196, 361, 550, 925
NheI GCTAGC 1 cut(s) 371
NlaIII CATG 9 cut(s) 184, 417, 512, 823, 863, 992, 1317, 1325, 1358
NlaIV GGNNCC 3 cut(s) 903, 951, 1201
NruI TCGCGA 1 cut(s) 238
NspI RCATGY 2 cut(s) 417, 512
PaeI GCATGC 1 cut(s) 417
PagI TCATGA 1 cut(s) 819
PctI GAATGC 2 cut(s) 675, 1084
PdmI GAANNNNTTC 1 cut(s) 544
PfeI GAWTC 5 cut(s) 13, 72, 689, 1018, 1229
PkrI GCNGC 2 cut(s) 370, 979
PleI GAGTC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 701
PspN4I GGNNCC 3 cut(s) 903, 951, 1201
PspPI GGNCC 2 cut(s) 902, 950
PvuII CAGCTG 1 cut(s) 583
RruI TCGCGA 1 cut(s) 238
RsaI GTAC 3 cut(s) 520, 1014, 1125
RsaNI GTAC 3 cut(s) 519, 1013, 1124
RseI CAYNNNNRTG 2 cut(s) 824, 1257
SaqAI TTAA 4 cut(s) 570, 702, 1185, 1338
SatI GCNGC 2 cut(s) 369, 978
Sau3AI GATC 5 cut(s) 130, 196, 361, 550, 925
Sau96I GGNCC 2 cut(s) 902, 950
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 1 cut(s) 1034
SfaNI GCATC 9 cut(s) 278, 439, 457, 591, 784, 784, 820, 854, 1015
SinI GGWCC 2 cut(s) 902, 950
SmiMI CAYNNNNRTG 2 cut(s) 824, 1257
SpeI ACTAGT 1 cut(s) 1100
SphI GCATGC 1 cut(s) 417
Sse9I AATT 9 cut(s) 110, 185, 461, 501, 540, 885, 1049, 1067, 1288
SspI AATATT 1 cut(s) 440
SspMI CTAG 3 cut(s) 372, 995, 1101
StyD4I CCNGG 1 cut(s) 1032
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 5 cut(s) 56, 518, 954, 1093, 1128
TaiI ACGT 1 cut(s) 1156
TaqI TCGA 4 cut(s) 75, 276, 304, 1332
TaqII GACCGA 1 cut(s) 344
TasI AATT 9 cut(s) 110, 185, 461, 501, 540, 885, 1049, 1067, 1288
TatI WGTACW 2 cut(s) 1012, 1123
TfiI GAWTC 5 cut(s) 13, 72, 689, 1018, 1229
Tru1I TTAA 4 cut(s) 570, 702, 1185, 1338
Tru9I TTAA 4 cut(s) 570, 702, 1185, 1338
TscAI CASTG 1 cut(s) 979
TseI GCWGC 2 cut(s) 368, 977
TspRI CASTG 1 cut(s) 979
VpaK11BI GGWCC 2 cut(s) 902, 950
XapI RAATTY 5 cut(s) 185, 461, 540, 1049, 1288
XceI RCATGY 2 cut(s) 417, 512
XcmI CCANNNNNNNNNTGG 3 cut(s) 1070, 1126, 1311
XmiI GTMKAC 1 cut(s) 1078
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 3 cut(s) 372, 995, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.