MD16G1260500.v1.1

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006733
Physical Location & Seq
Forward (+)
32083915 .. 32103593
19679 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1260500.v1.1.491

Sequence Viewer

Length: 1653 bp
ATGGCCTGCCTTCGTAGGCTGCTTCCTCTCTGCTCTTCCATCAACAGGACGAGCTTCCCATCTTCTGGTTCTGGAGCTCTGCAAGGTTTTCGAAGCTATGGAGCAATTGCTTCCGAGAAAACTCTGTTGAAATTTGGAGATGGAGAGACTCGACCTGAAGCTGATACTTCAGTTCTTAATGATGAGATAGCACAGATTCGACGTGAATTTGATGCTGCGAAGCAGAGTTTCCTCAAGATTCCAGCCGCACTTAAAGCAATGCCTAAAATGAATCCGAAAGGGATATATGTAAATAAAAACCTGAGATTGGACAATATTCAAGTTTATGGATTTGACTATGATTACACGCTGGTACATTATTCTGCCAATTTACAGAGCTTGATATATGATCTGGCAAAGGAGCATATGGTAAATGAGTTTCGGTACCCTGAGGTTTGCATGGCATTTAAGTATGATTCGACTTTTCCCATTAGAGGATTGTATTATGATAAGCTAAAAGGGTGCCTATTGAAGCTGGATTTTTTTGGGTCAATTGAGCCAGATGGATGCTACTATGGTCGTCGTAAGCTTAGCAGGAGGGAAATTGAAGAAATATATGGCACACGACGTATTGGTCGTGATCAGGCCCGGGGACTTGTTGGGTTGATGGATTTCTTTTGCTTTAGCGAGGCATGCCTCATTTCAGATATCGTGCAATATTTTGTTGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTAAATCGTGCAATTCAGCATGTTCATCGCAGTGGCTTGGTTCATAGAGGAATTCTTTCTGACCCCCATAGATATCTAGTAAAAAATGGACAACTGTTACACTTTCTCAAGATGCTCAGGGAGAAGGGAAAGAAACTATTCTTGCTGACTAACTCTCCATATTACTTTGTGGATGGAGGAATGACCTTTATGTTGGAGGATTCTAAGGAATACAGAGACTCCTGGAGGGAACTTTTTGATGTTGTGATTGCTAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTTCTATGACACGGAGAAGGATAATTTAGCTTTCAAGAAGGTGGATGAATTTCTTCCTAATAAAATATATTACCATGGATGCCTTAAATCCTTCCTTCATATTACCAAGTGGAAGGGACCAGAGGTGATGTACTTTGGAGACCACCTATTTAGCGACCTTAGGGGGCCTGCAAAAGCTGGCTGGCGCACTGCTGCTATAATTCATGAGCTAGAAGATGAAATACTTATTCAAAACAAGGATACTTACCGCTTGGAACAGGCAAAATTTCATATAGTACAAGAATTGCTTGGTAGATTGCACGCTACTGTTGCTTTTACTCAGAAGAGGGAAGCTTACACGTCACTGTTGGAGGAGTTAAATGAATGGAGGCAGGAGGCAAGTCACAAGATGAAAAGAATGTTTAATAGGTCCTTTGGGGCCACATTTCTTACGGATAAAGGTCAGGAATCTGCTTTTGCCTATCACATTCATCAGTATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTCCATGTGCCCTTCGATATTAAGATTATGCCACATCATGTGAAGGTTCCATCAAGCTTGTTCAAAAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

551

Amino Acids

64.11

Weight (kDa)

8.46

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 95 - 540 3.7e-147 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 612
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 423, 501
AccB7I CCANNNNNTGG 1 cut(s) 65
AciI CCGC 2 cut(s) 246, 1291
AcsI RAATTY 7 cut(s) 131, 206, 719, 798, 1091, 1307, 1549
AcuI CTGAAG 3 cut(s) 153, 177, 1590
AfaI GTAC 4 cut(s) 354, 425, 1175, 1320
AfiI CCNNNNNNNGG 4 cut(s) 45, 65, 307, 473
AflIII ACRYGT 1 cut(s) 1380
AgsI TTSAA 7 cut(s) 130, 320, 511, 587, 1078, 1274, 1642
AjiI CACGTC 2 cut(s) 203, 1383
AjnI CCWGG 1 cut(s) 968
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 3 cut(s) 140, 957, 1176
Ama87I CYCGRG 1 cut(s) 627
AoxI GGCC 4 cut(s) 3, 624, 1208, 1461
ApeKI GCWGC 3 cut(s) 19, 215, 1235
ApoI RAATTY 7 cut(s) 131, 206, 719, 798, 1091, 1307, 1549
Asp700I GAANNNNTTC 3 cut(s) 802, 884, 1095
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 1230
AspS9I GGNCC 5 cut(s) 625, 1160, 1208, 1452, 1461
AsuC2I CCSGG 2 cut(s) 628, 629
AsuHPI GGTGA 1 cut(s) 1180
AsuII TTCGAA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 627
AvaII GGWCC 2 cut(s) 1160, 1452
AxyI CCTNAGG 2 cut(s) 429, 1202
BaeGI GKGCMC 1 cut(s) 1590
BaeI ACNNNNGTAYC 2 cut(s) 156, 189
BanI GGYRCC 2 cut(s) 423, 501
BanII GRGCYC 1 cut(s) 79
BarI GAAGNNNNNNTAC 2 cut(s) 1248, 1280
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 3 cut(s) 6, 202, 1222
BccI CCATC 7 cut(s) 47, 67, 134, 536, 640, 914, 1636
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 755, 789
BciT130I CCWGG 1 cut(s) 970
BciVI GTATCC 1 cut(s) 1276
BclI TGATCA 1 cut(s) 619
BcnI CCSGG 2 cut(s) 628, 629
BcoDI GTCTC 3 cut(s) 140, 957, 1176
BfaI CTAG 2 cut(s) 824, 1253
BfuI GTATCC 1 cut(s) 1276
BisI GCNGC 4 cut(s) 20, 216, 246, 1236
BlpI GCTNAGC 2 cut(s) 569, 711
BlsI GCNGC 4 cut(s) 21, 217, 247, 1237
Bme1390I CCNGG 3 cut(s) 628, 629, 970
Bme18I GGWCC 2 cut(s) 1160, 1452
BmeT110I CYCGRG 1 cut(s) 627
BmgBI CACGTC 2 cut(s) 203, 1383
BmgT120I GGNCC 5 cut(s) 625, 1160, 1208, 1452, 1461
BmiI GGNNCC 7 cut(s) 425, 503, 1161, 1209, 1462, 1580, 1626
BmrFI CCNGG 3 cut(s) 628, 629, 970
BmsI GCATC 7 cut(s) 202, 536, 697, 715, 849, 1042, 1112
BpmI CTGGAG 2 cut(s) 93, 991
Bpu10I CCTNAGC 1 cut(s) 863
Bpu1102I GCTNAGC 2 cut(s) 569, 711
Bpu14I TTCGAA 1 cut(s) 91
BpuEI CTTGAG 2 cut(s) 218, 839
BpuMI CCSGG 2 cut(s) 628, 629
BsaBI GATNNNNATC 1 cut(s) 750
BsaI GGTCTC 1 cut(s) 1176
BsaJI CCNNGG 3 cut(s) 627, 628, 1117
BsaXI ACNNNNNCTCC 4 cut(s) 886, 916, 950, 980
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 65, 307, 473
Bse1I ACTGG 1 cut(s) 1536
Bse21I CCTNAGG 2 cut(s) 429, 1202
Bse3DI GCAATG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 1 cut(s) 970
BseDI CCNNGG 3 cut(s) 627, 628, 1117
BseGI GGATG 5 cut(s) 551, 925, 1033, 1093, 1127
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 4 cut(s) 45, 65, 307, 473
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 5 cut(s) 293, 420, 877, 1020, 1376
BseNI ACTGG 1 cut(s) 1536
BseRI GAGGAG 1 cut(s) 1409
BseSI GKGCMC 1 cut(s) 1590
BseXI GCAGC 3 cut(s) 6, 202, 1222
BshFI GGCC 4 cut(s) 5, 626, 1210, 1463
BshNI GGYRCC 2 cut(s) 423, 501
BsiHKAI GWGCWC 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 627
BsiSI CCGG 1 cut(s) 628
BslFI GGGAC 2 cut(s) 645, 1173
BslI CCNNNNNNNGG 4 cut(s) 45, 65, 307, 473
BsmAI GTCTC 3 cut(s) 140, 957, 1176
BsmFI GGGAC 2 cut(s) 645, 1173
BsnI GGCC 4 cut(s) 5, 626, 1210, 1463
Bso31I GGTCTC 1 cut(s) 1176
BsoBI CYCGRG 1 cut(s) 627
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 79, 1590
Bsp143I GATC 2 cut(s) 388, 619
Bsp1720I GCTNAGC 2 cut(s) 569, 711
Bsp19I CCATGG 1 cut(s) 1117
BspACI CCGC 2 cut(s) 246, 1291
BspANI GGCC 4 cut(s) 5, 626, 1210, 1463
BspCNI CTCAG 5 cut(s) 294, 421, 876, 1021, 1375
BspHI TCATGA 1 cut(s) 1246
BspLI GGNNCC 7 cut(s) 425, 503, 1161, 1209, 1462, 1580, 1626
BspQI GCTCTTC 1 cut(s) 40
BspT104I TTCGAA 1 cut(s) 91
BspT107I GGYRCC 2 cut(s) 423, 501
BspTNI GGTCTC 1 cut(s) 1176
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 1 cut(s) 1536
BssECI CCNNGG 3 cut(s) 627, 628, 1117
BssMI GATC 2 cut(s) 388, 619
BssT1I CCWWGG 1 cut(s) 1117
Bst2UI CCWGG 1 cut(s) 970
Bst4CI ACNGT 3 cut(s) 843, 1351, 1389
Bst6I CTCTTC 2 cut(s) 40, 1361
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 7 cut(s) 7, 673, 730, 1212, 1222, 1226, 1344
BstDEI CTNAG 9 cut(s) 302, 429, 569, 711, 863, 951, 1029, 1202, 1362
BstDSI CCRYGG 1 cut(s) 1117
BstF5I GGATG 5 cut(s) 551, 925, 1033, 1093, 1127
BstHHI GCGC 1 cut(s) 1230
BstKTI GATC 2 cut(s) 391, 622
BstMAI GTCTC 3 cut(s) 140, 957, 1176
BstMBI GATC 2 cut(s) 388, 619
BstMWI GCNNNNNNNGC 4 cut(s) 254, 672, 1352, 1585
BstNI CCWGG 1 cut(s) 970
BstNSI RCATGY 2 cut(s) 675, 770
BstSCI CCNGG 3 cut(s) 626, 627, 968
BstSLI GKGCMC 1 cut(s) 1590
BstV1I GCAGC 3 cut(s) 6, 202, 1222
Bsu36I CCTNAGG 2 cut(s) 429, 1202
BsuI GTATCC 1 cut(s) 1276
BsuRI GGCC 4 cut(s) 5, 626, 1210, 1463
BtgI CCRYGG 1 cut(s) 1117
BtgZI GCGATG 1 cut(s) 758
BtrI CACGTC 2 cut(s) 203, 1383
BtsCI GGATG 5 cut(s) 551, 925, 1033, 1093, 1127
BtsI GCAGTG 2 cut(s) 784, 1230
BtsIMutI CAGTG 3 cut(s) 784, 1230, 1385
Cac8I GCNNGC 7 cut(s) 7, 673, 730, 1212, 1222, 1226, 1344
CciI TCATGA 1 cut(s) 1246
CfoI GCGC 1 cut(s) 1230
Cfr13I GGNCC 5 cut(s) 625, 1160, 1208, 1452, 1461
Cfr9I CCCGGG 1 cut(s) 627
Csp6I GTAC 4 cut(s) 353, 424, 1174, 1319
CviAII CATG 8 cut(s) 439, 672, 767, 1118, 1247, 1575, 1583, 1616
CviQI GTAC 4 cut(s) 353, 424, 1174, 1319
DdeI CTNAG 9 cut(s) 302, 429, 569, 711, 863, 951, 1029, 1202, 1362
DpnI GATC 2 cut(s) 390, 621
DpnII GATC 2 cut(s) 388, 619
DrdI GACNNNNNNGTC 1 cut(s) 612
DseDI GACNNNNNNGTC 1 cut(s) 612
Eam1104I CTCTTC 2 cut(s) 40, 1361
EarI CTCTTC 2 cut(s) 40, 1361
Ecl136II GAGCTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 1117
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 1176
Eco32I GATATC 2 cut(s) 688, 821
Eco47I GGWCC 2 cut(s) 1160, 1452
Eco53kI GAGCTC 1 cut(s) 77
Eco57I CTGAAG 3 cut(s) 153, 177, 1590
Eco81I CCTNAGG 2 cut(s) 429, 1202
Eco88I CYCGRG 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 77
EcoO109I RGGNCCY 2 cut(s) 1208, 1452
EcoRI GAATTC 1 cut(s) 798
EcoRII CCWGG 1 cut(s) 968
EcoRV GATATC 2 cut(s) 688, 821
EcoT14I CCWWGG 1 cut(s) 1117
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 1117
FaeI CATG 8 cut(s) 442, 675, 770, 1121, 1250, 1578, 1586, 1619
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 2 cut(s) 645, 1173
FatI CATG 8 cut(s) 438, 671, 766, 1117, 1246, 1574, 1582, 1615
FauNDI CATATG 1 cut(s) 405
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 4 cut(s) 20, 216, 246, 1236
FokI GGATG 5 cut(s) 558, 932, 1020, 1100, 1134
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 4 cut(s) 20, 216, 246, 1236
FspBI CTAG 2 cut(s) 824, 1253
GlaI GCGC 1 cut(s) 1229
GluI GCNGC 4 cut(s) 20, 216, 246, 1236
GsuI CTGGAG 2 cut(s) 93, 991
HaeIII GGCC 4 cut(s) 5, 626, 1210, 1463
HapII CCGG 1 cut(s) 628
HhaI GCGC 1 cut(s) 1230
Hin1II CATG 8 cut(s) 442, 675, 770, 1121, 1250, 1578, 1586, 1619
Hin6I GCGC 1 cut(s) 1228
HinP1I GCGC 1 cut(s) 1228
HindIII AAGCTT 3 cut(s) 566, 1374, 1633
HinfI GANTC 8 cut(s) 148, 196, 238, 271, 455, 947, 965, 1490
HpaII CCGG 1 cut(s) 628
HphI GGTGA 1 cut(s) 1180
Hpy188I TCNGA 6 cut(s) 115, 276, 685, 808, 1030, 1365
Hpy188III TCNNGA 8 cut(s) 72, 235, 617, 743, 856, 1078, 1247, 1487
Hpy99I CGWCG 3 cut(s) 204, 564, 609
HpyAV CCTTC 9 cut(s) 20, 865, 1054, 1075, 1144, 1148, 1150, 1600, 1615
HpyCH4III ACNGT 3 cut(s) 843, 1351, 1389
HpyCH4IV ACGT 3 cut(s) 202, 607, 1382
HpyCH4V TGCA 7 cut(s) 82, 438, 694, 758, 1214, 1342, 1523
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 672, 1352, 1585
HpyF3I CTNAG 9 cut(s) 302, 429, 569, 711, 863, 951, 1029, 1202, 1362
HpySE526I ACGT 3 cut(s) 202, 607, 1382
Hsp92II CATG 8 cut(s) 442, 675, 770, 1121, 1250, 1578, 1586, 1619
HspAI GCGC 1 cut(s) 1228
KpnI GGTACC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 2 cut(s) 388, 619
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 4 cut(s) 74, 101, 400, 1584
Lsp1109I GCAGC 3 cut(s) 6, 202, 1222
LweI GCATC 7 cut(s) 202, 536, 697, 715, 849, 1042, 1112
MaeI CTAG 2 cut(s) 824, 1253
MaeII ACGT 3 cut(s) 202, 607, 1382
MaeIII GTNAC 3 cut(s) 843, 1383, 1424
MalI GATC 2 cut(s) 390, 621
MboI GATC 2 cut(s) 388, 619
MboII GAAGA 6 cut(s) 27, 54, 599, 1088, 1268, 1378
MfeI CAATTG 2 cut(s) 105, 531
MhlI GDGCHC 2 cut(s) 79, 1590
MlyI GAGTC 2 cut(s) 142, 959
MmeI TCCRAC 2 cut(s) 921, 1371
MroXI GAANNNNTTC 3 cut(s) 802, 884, 1095
MseI TTAA 7 cut(s) 177, 252, 447, 1128, 1400, 1446, 1599
MslI CAYNNNNRTG 2 cut(s) 777, 1518
MspI CCGG 1 cut(s) 628
MspR9I CCNGG 3 cut(s) 628, 629, 970
MunI CAATTG 2 cut(s) 105, 531
MvaI CCWGG 1 cut(s) 970
MwoI GCNNNNNNNGC 4 cut(s) 254, 672, 1352, 1585
NciI CCSGG 2 cut(s) 628, 629
NcoI CCATGG 1 cut(s) 1117
NdeI CATATG 1 cut(s) 405
NdeII GATC 2 cut(s) 388, 619
NlaIII CATG 8 cut(s) 442, 675, 770, 1121, 1250, 1578, 1586, 1619
NlaIV GGNNCC 7 cut(s) 425, 503, 1161, 1209, 1462, 1580, 1626
NmuCI GTSAC 2 cut(s) 1383, 1424
NspI RCATGY 2 cut(s) 675, 770
NspV TTCGAA 1 cut(s) 91
PaeI GCATGC 1 cut(s) 675
PagI TCATGA 1 cut(s) 1246
PciSI GCTCTTC 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 613
PdmI GAANNNNTTC 3 cut(s) 802, 884, 1095
PfeI GAWTC 6 cut(s) 196, 238, 271, 455, 947, 1490
PflMI CCANNNNNTGG 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 968
PkrI GCNGC 4 cut(s) 21, 217, 247, 1237
PleI GAGTC 2 cut(s) 142, 959
PpsI GAGTC 2 cut(s) 142, 959
PpuMI RGGWCCY 1 cut(s) 1452
Psp124BI GAGCTC 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 1452
Psp6I CCWGG 1 cut(s) 968
PspGI CCWGG 1 cut(s) 968
PspN4I GGNNCC 7 cut(s) 425, 503, 1161, 1209, 1462, 1580, 1626
PspPI GGNCC 5 cut(s) 625, 1160, 1208, 1452, 1461
PspPPI RGGWCCY 1 cut(s) 1452
RsaI GTAC 4 cut(s) 354, 425, 1175, 1320
RsaNI GTAC 4 cut(s) 353, 424, 1174, 1319
RseI CAYNNNNRTG 2 cut(s) 777, 1518
SacI GAGCTC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 7 cut(s) 177, 252, 447, 1128, 1400, 1446, 1599
SatI GCNGC 4 cut(s) 20, 216, 246, 1236
Sau3AI GATC 2 cut(s) 388, 619
Sau96I GGNCC 5 cut(s) 625, 1160, 1208, 1452, 1461
SchI GAGTC 2 cut(s) 142, 959
ScrFI CCNGG 3 cut(s) 628, 629, 970
SduI GDGCHC 2 cut(s) 79, 1590
SfaNI GCATC 7 cut(s) 202, 536, 697, 715, 849, 1042, 1112
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 2 cut(s) 1160, 1452
SmaI CCCGGG 1 cut(s) 629
SmiMI CAYNNNNRTG 2 cut(s) 777, 1518
SmlI CTYRAG 2 cut(s) 233, 854
SmoI CTYRAG 2 cut(s) 233, 854
SphI GCATGC 1 cut(s) 675
SsiI CCGC 2 cut(s) 246, 1291
SspI AATATT 2 cut(s) 316, 698
SspMI CTAG 2 cut(s) 824, 1253
SstI GAGCTC 1 cut(s) 79
StyD4I CCNGG 3 cut(s) 626, 627, 968
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 3 cut(s) 843, 1351, 1389
TaiI ACGT 3 cut(s) 205, 610, 1385
TaqI TCGA 5 cut(s) 91, 151, 199, 458, 1593
TatI WGTACW 2 cut(s) 1173, 1318
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 6 cut(s) 196, 238, 271, 455, 947, 1490
Tru1I TTAA 7 cut(s) 177, 252, 447, 1128, 1400, 1446, 1599
Tru9I TTAA 7 cut(s) 177, 252, 447, 1128, 1400, 1446, 1599
TscAI CASTG 3 cut(s) 784, 1237, 1392
TseFI GTSAC 2 cut(s) 1383, 1424
TseI GCWGC 3 cut(s) 19, 215, 1235
Tsp45I GTSAC 2 cut(s) 1383, 1424
TspGWI ACGGA 3 cut(s) 1031, 1070, 1490
TspMI CCCGGG 1 cut(s) 627
TspRI CASTG 3 cut(s) 784, 1237, 1392
Van91I CCANNNNNTGG 1 cut(s) 65
VpaK11BI GGWCC 2 cut(s) 1160, 1452
XapI RAATTY 7 cut(s) 131, 206, 719, 798, 1091, 1307, 1549
XceI RCATGY 2 cut(s) 675, 770
XcmI CCANNNNNNNNNTGG 1 cut(s) 1572
XmaI CCCGGG 1 cut(s) 627
XmnI GAANNNNTTC 3 cut(s) 802, 884, 1095
XspI CTAG 2 cut(s) 824, 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.