Rh4BG122800

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006733
Physical Location & Seq
Forward (+)
20543292 .. 20555251
11960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG122800.1

Sequence Viewer

Length: 1677 bp
ATGTTAAAATGGCGGTACTTGATTTTCGCATACACCACTAAACAAGTTGAGCTTCCCATGGCCTTGTTTCGCAGGCTGCTCCCTCTCTCCTCTTCCATCAACAGGACGAGCTTTTGCTCATCTGGAGCTCTACAAGGTTTTCGGAGTTATGGAGCCATTGCTACACCCGAGCCAACCGTGTTGAAATTTGAGAATGGAGAGACTGCTGATGATGAGATAGCAAAGATTCGGCGTGAATTTGATGCCGCAAAGCAGAGTTTCCTCAAGATTCCACAGGCACTTAAAGAAGTGCCGAAGATGAATCCGGAAGGGATATATGTAAATAAAAACCTGAGGTTGGACAGTATTCAAGTTTATGGATTTGACTATGACTACACGCTGGCGCATTACTCGGCAGATTTACAGAACTTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGATCCGGTATCCTGAGGTTTGCATGGAATTTAAGTACGATCCTACTTTTCCCATTAGAGGACTGTGCTATGACAAATTGACAGGGTGTCTCTTGAAGCTGGACTTTTTTGGGTCAATTGAGCCAGGTGGATGCTACTATGGTCGTCGAAAGCTTAGTAGGAAGGAAATGGATGAGATATATGGCACAAGGCGTATTGGTCGTGATCAAGCAGGAAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGTGAGGCATGCCTCATTGCAGACATCGTACAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTAAATCGTGCGATTCAGCATGTTCACCGTACTGGCCTTGTTCATACAGGAATTCTTTCTGATCCCCATAAATATCTTGTTAAAAATGGCCAGCTGTTACACTTTCTGAAGATGCTCAAGGAAAAAGGAAAGAAACTATTCTTGCTGACTAACTCTCCATATTACTTTGTGGATGGAGGATTGCGTTTTTTGTTGGAGGATTCTTTGGGTTGTAATGACTCTTGGAGGGAACTTTTTGATGTTGTGATTGCCAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTGTTATGATGCAGAGAAGGATAATTTAGCTTTCACAAAGGTGGATGCGCTTCTTCCTAATAAAATTTATTACCATGGATGCCTGAAATCATTCCTTCAAATTACCAAGTGGAAGGGACCAGAGGTGATATACTTTGGTGATCACCTCTTTAGCGACCTAAGGGGACCATCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAAGATGAAATACTTATACAAAATGAGGATGCTTACCGCGTGGAGCAGGCGAAATTTCATATATTCCAAGAATTGATTGGTAGACTGCATTCAACGGTAGCTAATAGTCAGAAAAGTGAGGCCAAGTTACTGTTGGACGAGCTAAATGAGTGCAGGCGTAAGGCAAGCTACAAGATGAAGAGAATGTTCAATAGCTCTTTCGGAGCCACCTTCCTTACGGATAAAGTTCAAGAATCTGCTTTCGCGTATCATATTCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTTCATGCACCCTTCGATATTAAAATTATGCCACATCATGTGAAGGTTTCATCCAGCTTGTTCAAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

558

Amino Acids

64.53

Weight (kDa)

7.57

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 105 - 549 1e-147 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1366
AccII CGCG 2 cut(s) 1323, 1529
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 3 cut(s) 13, 246, 1321
AclWI GGATC 3 cut(s) 442, 479, 833
AcoI YGGCCR 1 cut(s) 865
AcsI RAATTY 8 cut(s) 185, 236, 473, 749, 828, 1137, 1337, 1576
AcuI CTGAAG 2 cut(s) 905, 1617
AfaI GTAC 4 cut(s) 17, 482, 723, 808
AfiI CCNNNNNNNGG 3 cut(s) 102, 337, 503
AgsI TTSAA 8 cut(s) 184, 350, 541, 1172, 1377, 1474, 1514, 1669
AhdI GACNNNNNGTC 1 cut(s) 531
AjnI CCWGG 1 cut(s) 568
AleI CACNNNNGTG 1 cut(s) 1112
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 2 cut(s) 194, 539
AlwI GGATC 3 cut(s) 442, 479, 833
Ama87I CYCGRG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 4 cut(s) 60, 811, 865, 1404
ApeKI GCWGC 2 cut(s) 76, 1265
ApoI RAATTY 8 cut(s) 185, 236, 473, 749, 828, 1137, 1337, 1576
Asp700I GAANNNNTTC 3 cut(s) 832, 914, 1163
AspLEI GCGC 3 cut(s) 385, 1123, 1260
AspS9I GGNCC 2 cut(s) 1190, 1238
AsuHPI GGTGA 4 cut(s) 794, 1208, 1210, 1223
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 1190, 1238
AxyI CCTNAGG 3 cut(s) 332, 459, 1232
BalI TGGCCA 1 cut(s) 867
BanII GRGCYC 1 cut(s) 130
BarI GAAGNNNNNNTAC 2 cut(s) 1278, 1310
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 63, 1252
BccI CCATC 4 cut(s) 104, 670, 944, 1249
BciT130I CCWGG 1 cut(s) 570
BciVI GTATCC 1 cut(s) 465
BclI TGATCA 2 cut(s) 649, 1213
BcoDI GTCTC 2 cut(s) 194, 539
BfaI CTAG 1 cut(s) 1283
BfuI GTATCC 1 cut(s) 465
BisI GCNGC 3 cut(s) 77, 246, 1266
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 3 cut(s) 78, 247, 1267
Bme1390I CCNGG 1 cut(s) 570
Bme18I GGWCC 2 cut(s) 1190, 1238
BmeRI GACNNNNNGTC 1 cut(s) 531
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 1190, 1238
BmiI GGNNCC 4 cut(s) 154, 1191, 1239, 1489
BmrFI CCNGG 1 cut(s) 570
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 248, 878
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 2 cut(s) 57, 1147
BsaWI WCCGGW 2 cut(s) 304, 450
BsaXI ACNNNNNCTCC 2 cut(s) 916, 946
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 337, 503
Bse1I ACTGG 2 cut(s) 814, 1563
Bse21I CCTNAGG 3 cut(s) 332, 459, 1232
Bse3DI GCAATG 2 cut(s) 156, 708
Bse8I GATNNNNATC 1 cut(s) 780
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 57, 1147
BseGI GGATG 9 cut(s) 581, 622, 742, 955, 1063, 1123, 1157, 1318, 1655
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 3 cut(s) 102, 337, 503
BseMI GCAATG 2 cut(s) 156, 708
BseMII CTCAG 3 cut(s) 323, 450, 1050
BseNI ACTGG 2 cut(s) 814, 1563
BseRI GAGGAG 1 cut(s) 79
BseXI GCAGC 2 cut(s) 63, 1252
BsgI GTGCAG 1 cut(s) 1456
Bsh1236I CGCG 2 cut(s) 1323, 1529
BshFI GGCC 4 cut(s) 62, 813, 867, 1406
BsiHKAI GWGCWC 1 cut(s) 130
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 2 cut(s) 305, 451
BslFI GGGAC 2 cut(s) 1203, 1251
BslI CCNNNNNNNGG 3 cut(s) 102, 337, 503
BsmAI GTCTC 2 cut(s) 194, 539
BsmFI GGGAC 2 cut(s) 1203, 1251
BsmI GAATGC 1 cut(s) 1372
BsnI GGCC 4 cut(s) 62, 813, 867, 1406
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 130
Bsp13I TCCGGA 1 cut(s) 304
Bsp143I GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
Bsp1720I GCTNAGC 1 cut(s) 741
Bsp19I CCATGG 2 cut(s) 57, 1147
BspACI CCGC 3 cut(s) 13, 246, 1321
BspANI GGCC 4 cut(s) 62, 813, 867, 1406
BspCNI CTCAG 3 cut(s) 324, 451, 1051
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 2 cut(s) 1323, 1529
BspLI GGNNCC 4 cut(s) 154, 1191, 1239, 1489
BspPI GGATC 3 cut(s) 442, 479, 833
BsrDI GCAATG 2 cut(s) 156, 708
BsrI ACTGG 2 cut(s) 814, 1563
BssECI CCNNGG 2 cut(s) 57, 1147
BssMI GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
BssT1I CCWWGG 2 cut(s) 57, 1147
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 6 cut(s) 178, 344, 510, 806, 1381, 1416
Bst6I CTCTTC 2 cut(s) 97, 1457
BstC8I GCNNGC 8 cut(s) 74, 381, 703, 760, 869, 1332, 1439, 1450
BstDEI CTNAG 7 cut(s) 332, 426, 459, 599, 741, 1059, 1232
BstDSI CCRYGG 2 cut(s) 57, 1147
BstF5I GGATG 9 cut(s) 581, 622, 742, 955, 1063, 1123, 1157, 1318, 1655
BstFNI CGCG 2 cut(s) 1323, 1529
BstHHI GCGC 3 cut(s) 385, 1123, 1260
BstKTI GATC 6 cut(s) 421, 450, 487, 652, 841, 1216
BstMAI GTCTC 2 cut(s) 194, 539
BstMBI GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 2 cut(s) 705, 800
BstSCI CCNGG 1 cut(s) 568
BstUI CGCG 2 cut(s) 1323, 1529
BstV1I GCAGC 2 cut(s) 63, 1252
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
Bsu36I CCTNAGG 3 cut(s) 332, 459, 1232
BsuI GTATCC 1 cut(s) 465
BsuRI GGCC 4 cut(s) 62, 813, 867, 1406
BtgI CCRYGG 2 cut(s) 57, 1147
BtsCI GGATG 9 cut(s) 581, 622, 742, 955, 1063, 1123, 1157, 1318, 1655
BtsI GCAGTG 1 cut(s) 1260
BtsIMutI CAGTG 1 cut(s) 1260
Cac8I GCNNGC 8 cut(s) 74, 381, 703, 760, 869, 1332, 1439, 1450
CfoI GCGC 3 cut(s) 385, 1123, 1260
Cfr13I GGNCC 2 cut(s) 1190, 1238
Csp6I GTAC 4 cut(s) 16, 481, 722, 807
CviAII CATG 9 cut(s) 58, 469, 702, 797, 1148, 1277, 1602, 1610, 1643
CviQI GTAC 4 cut(s) 16, 481, 722, 807
DdeI CTNAG 7 cut(s) 332, 426, 459, 599, 741, 1059, 1232
DpnI GATC 6 cut(s) 420, 449, 486, 651, 840, 1215
DpnII GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
DriI GACNNNNNGTC 1 cut(s) 531
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 2 cut(s) 97, 1457
Eam1105I GACNNNNNGTC 1 cut(s) 531
EarI CTCTTC 2 cut(s) 97, 1457
Ecl136II GAGCTC 1 cut(s) 128
Eco130I CCWWGG 2 cut(s) 57, 1147
Eco24I GRGCYC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 1190, 1238
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 2 cut(s) 905, 1617
Eco81I CCTNAGG 3 cut(s) 332, 459, 1232
Eco88I CYCGRG 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 828
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 2 cut(s) 57, 1147
EcoT38I GRGCYC 1 cut(s) 130
ErhI CCWWGG 2 cut(s) 57, 1147
FaeI CATG 9 cut(s) 61, 472, 705, 800, 1151, 1280, 1605, 1613, 1646
FalI AAGNNNNNCTT 6 cut(s) 36, 68, 533, 565, 1590, 1622
FaqI GGGAC 2 cut(s) 1203, 1251
FatI CATG 9 cut(s) 57, 468, 701, 796, 1147, 1276, 1601, 1609, 1642
FauNDI CATATG 1 cut(s) 435
FbaI TGATCA 2 cut(s) 649, 1213
FblI GTMKAC 1 cut(s) 1366
Fnu4HI GCNGC 3 cut(s) 77, 246, 1266
FokI GGATG 9 cut(s) 588, 629, 749, 962, 1050, 1130, 1164, 1325, 1642
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 77, 246, 1266
FspBI CTAG 1 cut(s) 1283
GlaI GCGC 3 cut(s) 384, 1122, 1259
GluI GCNGC 3 cut(s) 77, 246, 1266
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 4 cut(s) 62, 813, 867, 1406
HapII CCGG 2 cut(s) 305, 451
HhaI GCGC 3 cut(s) 385, 1123, 1260
Hin1II CATG 9 cut(s) 61, 472, 705, 800, 1151, 1280, 1605, 1613, 1646
Hin6I GCGC 3 cut(s) 383, 1121, 1258
HinP1I GCGC 3 cut(s) 383, 1121, 1258
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 226, 268, 301, 790, 977, 995, 1517
HpaII CCGG 2 cut(s) 305, 451
HphI GGTGA 4 cut(s) 794, 1208, 1210, 1223
Hpy166II GTNNAC 2 cut(s) 802, 1367
Hpy188I TCNGA 6 cut(s) 144, 838, 885, 1060, 1395, 1487
Hpy188III TCNNGA 8 cut(s) 123, 265, 305, 458, 538, 647, 773, 1514
Hpy8I GTNNAC 2 cut(s) 802, 1367
Hpy99I CGWCG 1 cut(s) 594
HpyAV CCTTC 8 cut(s) 302, 601, 1084, 1178, 1180, 1504, 1627, 1642
HpyCH4III ACNGT 6 cut(s) 178, 344, 510, 806, 1381, 1416
HpyCH4V TGCA 7 cut(s) 468, 713, 1085, 1372, 1437, 1550, 1613
HpyF3I CTNAG 7 cut(s) 332, 426, 459, 599, 741, 1059, 1232
Hsp92II CATG 9 cut(s) 61, 472, 705, 800, 1151, 1280, 1605, 1613, 1646
HspAI GCGC 3 cut(s) 383, 1121, 1258
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 2 cut(s) 649, 1213
Kzo9I GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
LmnI GCTCC 6 cut(s) 84, 125, 152, 430, 1327, 1487
Lsp1109I GCAGC 2 cut(s) 63, 1252
MaeI CTAG 1 cut(s) 1283
MaeIII GTNAC 2 cut(s) 873, 1410
MalI GATC 6 cut(s) 420, 449, 486, 651, 840, 1215
MboI GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
MboII GAAGA 7 cut(s) 84, 307, 676, 898, 1118, 1298, 1474
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 867
MluNI TGGCCA 1 cut(s) 867
MlyI GAGTC 1 cut(s) 989
MmeI TCCRAC 3 cut(s) 318, 951, 1398
Mox20I TGGCCA 1 cut(s) 867
MroI TCCGGA 1 cut(s) 304
MroXI GAANNNNTTC 3 cut(s) 832, 914, 1163
MscI TGGCCA 1 cut(s) 867
MseI TTAA 6 cut(s) 5, 282, 477, 858, 1626, 1675
MslI CAYNNNNRTG 2 cut(s) 1112, 1545
Msp20I TGGCCA 1 cut(s) 867
MspA1I CMGCKG 1 cut(s) 871
MspI CCGG 2 cut(s) 305, 451
MspR9I CCNGG 1 cut(s) 570
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 1372
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 2 cut(s) 1323, 1529
NcoI CCATGG 2 cut(s) 57, 1147
NdeI CATATG 1 cut(s) 435
NdeII GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
NlaIII CATG 9 cut(s) 61, 472, 705, 800, 1151, 1280, 1605, 1613, 1646
NlaIV GGNNCC 4 cut(s) 154, 1191, 1239, 1489
NmeAIII GCCGAG 1 cut(s) 371
NspI RCATGY 2 cut(s) 705, 800
OliI CACNNNNGTG 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 705
PctI GAATGC 1 cut(s) 1372
PdmI GAANNNNTTC 3 cut(s) 832, 914, 1163
PfeI GAWTC 6 cut(s) 226, 268, 301, 790, 977, 1517
PkrI GCNGC 3 cut(s) 78, 247, 1267
PleI GAGTC 1 cut(s) 989
PpsI GAGTC 1 cut(s) 989
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 4 cut(s) 154, 1191, 1239, 1489
PspPI GGNCC 2 cut(s) 1190, 1238
PsuI RGATCY 1 cut(s) 447
PvuII CAGCTG 1 cut(s) 871
RsaI GTAC 4 cut(s) 17, 482, 723, 808
RsaNI GTAC 4 cut(s) 16, 481, 722, 807
RseI CAYNNNNRTG 2 cut(s) 1112, 1545
SacI GAGCTC 1 cut(s) 130
SaqAI TTAA 6 cut(s) 5, 282, 477, 858, 1626, 1675
SatI GCNGC 3 cut(s) 77, 246, 1266
Sau3AI GATC 6 cut(s) 418, 447, 484, 649, 838, 1213
Sau96I GGNCC 2 cut(s) 1190, 1238
SchI GAGTC 1 cut(s) 989
ScrFI CCNGG 1 cut(s) 570
SduI GDGCHC 1 cut(s) 130
SinI GGWCC 2 cut(s) 1190, 1238
SmiMI CAYNNNNRTG 2 cut(s) 1112, 1545
SmlI CTYRAG 2 cut(s) 263, 893
SmoI CTYRAG 2 cut(s) 263, 893
SphI GCATGC 1 cut(s) 705
SsiI CCGC 3 cut(s) 13, 246, 1321
SspI AATATT 1 cut(s) 728
SspMI CTAG 1 cut(s) 1283
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 1 cut(s) 568
StyI CCWWGG 2 cut(s) 57, 1147
TaaI ACNGT 6 cut(s) 178, 344, 510, 806, 1381, 1416
TaqI TCGA 2 cut(s) 592, 1620
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 6 cut(s) 226, 268, 301, 790, 977, 1517
Tru1I TTAA 6 cut(s) 5, 282, 477, 858, 1626, 1675
Tru9I TTAA 6 cut(s) 5, 282, 477, 858, 1626, 1675
TscAI CASTG 1 cut(s) 1267
TseI GCWGC 2 cut(s) 76, 1265
TspDTI ATGAA 9 cut(s) 314, 809, 1293, 1305, 1331, 1475, 1529, 1598, 1644
TspGWI ACGGA 2 cut(s) 1061, 1517
TspRI CASTG 1 cut(s) 1267
VpaK11BI GGWCC 2 cut(s) 1190, 1238
XapI RAATTY 8 cut(s) 185, 236, 473, 749, 828, 1137, 1337, 1576
XceI RCATGY 2 cut(s) 705, 800
XcmI CCANNNNNNNNNTGG 3 cut(s) 1358, 1414, 1599
XmiI GTMKAC 1 cut(s) 1366
XmnI GAANNNNTTC 3 cut(s) 832, 914, 1163
XspI CTAG 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.