Rw4G010440

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006733
Physical Location & Seq
Forward (+)
24240529 .. 24250513
9985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010440.1

Sequence Viewer

Length: 1677 bp
ATGGCCTTGTTTCGCAGGCTGCTTCATCCTCTCTGCTCTTCCATCAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGATAGGACGAGCTTTTGCTCATCTGGAGCTCTACAAGGTTTTCGGAGTTATGGAGCCATTGATACACCCGAGCCAACCGTGTTGAAATTTGAGAATGGAGAGACGGCTGATTCTGTGACTGCTGATGATGAGATAGCAAAGATTCGGCATGAATTTGATGCCGCAAAGCAGAGTTTCCTCAAGATTCCGCAGGCACTTCAAGAATTGCCGAAGATGAATCCGGAAGGGATATATGTAAATAAAAACCTGAGGTTGGACAGTATTCAAGTTTATGGATTTGACTATGACTACACGCTGGTGCATTACTCGGCAGATTTACAGAACTTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGTTCCGGTATCCTCATGTTTGCATGGAATTTAAGTACGATCCTACTTTTCCCATTAGAGGACTGTGCTATGACAAATTGAAAGGGTGTCTCTTGAAGCTGGACTTTTTTGGGTCAATTGAGCCAGATGGATGCTACTATGGTCGTCGAAAGCTTAGTAGGAAGGAAATAGATGAGATATATGGCACAAGGCGTATCGGTCGTGATCAAGCAGGAAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGTGAGGCATGCCTGATTGCAGACATCGTACAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTAAATCGTGCGATTCAGCATGTTCACCATACAGGCCTTGTTCATAGAGGAATTCTTTCTGATCCCCGTAAATATCTTGTTAAAAATGACCAGCTGTTACACTTTCTGAAGATGCTCAGGGAAAAAGGAAAGAAACTTTTCTTGCTGACTAACTCTCCATATTACTTTGTGGATAGAGGAATGCGTTTTTTGTTGGAGGATTCTTTGGGTTGTAATGACTCTTGGAGGGAACTTTTTGACGTTGTGATTGCCAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTGTTATGATGCAGAGAAGGATAATTTAGCTTTCATGAAGGTGGATGCGTTTCTTCCTAATAAAATTTATTACCACGGATGCCTGAAATCATTCCTTCAAATTACTAAGTGGAAGGGACCAGAGGTGATATACTTTGGTGATCACCTCTTTAGCGACCTAAGGGGACCATCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAAGATGAAATACGTATACAAAATGAGGATGCTTACCGCGTGGAGCAGGCAAAATTTCATATATTCCAAGAATTGCTTGGTAGACTGCATGCAACGGTAGCTAATAGTCAGAAAAGTGAGGCCAAGTTACTGTTGGAGGAGCTAAATGAGTGCAGGCGTAAGGCAAGCAACAAGATGAAGAGAATGTTCAATAGCTCTTTCGGAGCCACTTTCCTTACGGATAAAGGTCAAGAATCTGCTTTCTCGTATCATATTCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTTCATGCACCCTACGATATTAAGATTATGCCACATCATGTGAAGGTTTCATCCATCTTGTTCAAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

558

Amino Acids

64.6

Weight (kDa)

6.73

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 105 - 549 3.1e-149 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1300, 1366
AccII CGCG 1 cut(s) 1323
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 3 cut(s) 246, 272, 1321
AclWI GGATC 2 cut(s) 479, 833
AcsI RAATTY 8 cut(s) 170, 236, 473, 749, 828, 1137, 1337, 1576
AcuI CTGAAG 2 cut(s) 905, 1617
AfaI GTAC 2 cut(s) 482, 723
AfiI CCNNNNNNNGG 2 cut(s) 337, 503
AgsI TTSAA 8 cut(s) 169, 284, 350, 526, 541, 1172, 1474, 1669
AleI CACNNNNGTG 1 cut(s) 380
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 179, 539
AlwI GGATC 2 cut(s) 479, 833
Ama87I CYCGRG 1 cut(s) 152
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 3 cut(s) 3, 811, 1404
ApeKI GCWGC 2 cut(s) 19, 1265
ApoI RAATTY 8 cut(s) 170, 236, 473, 749, 828, 1137, 1337, 1576
Asp700I GAANNNNTTC 3 cut(s) 832, 914, 1163
AspLEI GCGC 1 cut(s) 1260
AspS9I GGNCC 2 cut(s) 1190, 1238
AsuHPI GGTGA 4 cut(s) 794, 1208, 1210, 1223
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 1190, 1238
AxyI CCTNAGG 2 cut(s) 332, 1232
BanII GRGCYC 1 cut(s) 115
BarI GAAGNNNNNNTAC 2 cut(s) 1278, 1310
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 6, 1252
BccI CCATC 5 cut(s) 50, 566, 670, 1249, 1667
BceAI ACGGC 1 cut(s) 204
BciVI GTATCC 1 cut(s) 465
BclI TGATCA 2 cut(s) 649, 1213
BcoDI GTCTC 2 cut(s) 179, 539
BfaI CTAG 1 cut(s) 1283
BfuI GTATCC 1 cut(s) 465
BisI GCNGC 3 cut(s) 20, 246, 1266
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 3 cut(s) 21, 247, 1267
Bme18I GGWCC 2 cut(s) 1190, 1238
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 2 cut(s) 1190, 1238
BmiI GGNNCC 4 cut(s) 139, 1191, 1239, 1489
BpmI CTGGAG 1 cut(s) 129
Bpu10I CCTNAGC 2 cut(s) 426, 893
Bpu1102I GCTNAGC 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 1298
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 1147
BsaWI WCCGGW 2 cut(s) 304, 450
BsaXI ACNNNNNCTCC 2 cut(s) 916, 946
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 503
Bse1I ACTGG 1 cut(s) 1563
Bse21I CCTNAGG 2 cut(s) 332, 1232
Bse8I GATNNNNATC 1 cut(s) 780
BseAI TCCGGA 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 1147
BseGI GGATG 8 cut(s) 25, 581, 742, 1063, 1123, 1157, 1318, 1655
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 337, 503
BseMII CTCAG 3 cut(s) 323, 907, 1050
BseNI ACTGG 1 cut(s) 1563
BseRI GAGGAG 1 cut(s) 1436
BseXI GCAGC 2 cut(s) 6, 1252
BsgI GTGCAG 1 cut(s) 1456
Bsh1236I CGCG 1 cut(s) 1323
Bsh1285I CGRYCG 1 cut(s) 646
BshFI GGCC 3 cut(s) 5, 813, 1406
BsiEI CGRYCG 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 2 cut(s) 305, 451
BslFI GGGAC 2 cut(s) 1203, 1251
BslI CCNNNNNNNGG 2 cut(s) 337, 503
BsmAI GTCTC 2 cut(s) 179, 539
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 1203, 1251
BsmI GAATGC 1 cut(s) 963
BsnI GGCC 3 cut(s) 5, 813, 1406
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 115
Bsp13I TCCGGA 1 cut(s) 304
Bsp143I GATC 5 cut(s) 418, 484, 649, 838, 1213
Bsp1720I GCTNAGC 1 cut(s) 741
BspACI CCGC 3 cut(s) 246, 272, 1321
BspANI GGCC 3 cut(s) 5, 813, 1406
BspCNI CTCAG 3 cut(s) 324, 906, 1051
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 1 cut(s) 1323
BspHI TCATGA 1 cut(s) 1107
BspLI GGNNCC 4 cut(s) 139, 1191, 1239, 1489
BspPI GGATC 2 cut(s) 479, 833
BspQI GCTCTTC 1 cut(s) 43
BsrI ACTGG 1 cut(s) 1563
BssECI CCNNGG 1 cut(s) 1147
BssMI GATC 5 cut(s) 418, 484, 649, 838, 1213
BssNAI GTATAC 1 cut(s) 1301
Bst1107I GTATAC 1 cut(s) 1301
Bst4CI ACNGT 5 cut(s) 163, 344, 510, 1381, 1416
Bst6I CTCTTC 2 cut(s) 43, 1457
BstBAI YACGTR 1 cut(s) 1298
BstC8I GCNNGC 8 cut(s) 17, 276, 703, 760, 1332, 1374, 1439, 1450
BstDEI CTNAG 8 cut(s) 332, 426, 599, 741, 893, 1059, 1179, 1232
BstDSI CCRYGG 1 cut(s) 1147
BstF5I GGATG 8 cut(s) 25, 581, 742, 1063, 1123, 1157, 1318, 1655
BstFNI CGCG 1 cut(s) 1323
BstHHI GCGC 1 cut(s) 1260
BstKTI GATC 5 cut(s) 421, 487, 652, 841, 1216
BstMAI GTCTC 2 cut(s) 179, 539
BstMBI GATC 5 cut(s) 418, 484, 649, 838, 1213
BstMCI CGRYCG 1 cut(s) 646
BstMWI GCNNNNNNNGC 1 cut(s) 1382
BstNSI RCATGY 3 cut(s) 705, 800, 1376
BstSNI TACGTA 1 cut(s) 1298
BstUI CGCG 1 cut(s) 1323
BstV1I GCAGC 2 cut(s) 6, 1252
BstZ17I GTATAC 1 cut(s) 1301
Bsu36I CCTNAGG 2 cut(s) 332, 1232
BsuI GTATCC 1 cut(s) 465
BsuRI GGCC 3 cut(s) 5, 813, 1406
BtgI CCRYGG 1 cut(s) 1147
BtsCI GGATG 8 cut(s) 25, 581, 742, 1063, 1123, 1157, 1318, 1655
BtsI GCAGTG 1 cut(s) 1260
BtsIMutI CAGTG 1 cut(s) 1260
Cac8I GCNNGC 8 cut(s) 17, 276, 703, 760, 1332, 1374, 1439, 1450
CciI TCATGA 1 cut(s) 1107
CfoI GCGC 1 cut(s) 1260
Cfr13I GGNCC 2 cut(s) 1190, 1238
Csp6I GTAC 2 cut(s) 481, 722
CviQI GTAC 2 cut(s) 481, 722
DdeI CTNAG 8 cut(s) 332, 426, 599, 741, 893, 1059, 1179, 1232
DpnI GATC 5 cut(s) 420, 486, 651, 840, 1215
DpnII GATC 5 cut(s) 418, 484, 649, 838, 1213
Eam1104I CTCTTC 2 cut(s) 43, 1457
EarI CTCTTC 2 cut(s) 43, 1457
Ecl136II GAGCTC 1 cut(s) 113
Eco105I TACGTA 1 cut(s) 1298
Eco147I AGGCCT 1 cut(s) 813
Eco24I GRGCYC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 1190, 1238
Eco53kI GAGCTC 1 cut(s) 113
Eco57I CTGAAG 2 cut(s) 905, 1617
Eco81I CCTNAGG 2 cut(s) 332, 1232
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 828
EcoT38I GRGCYC 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 179
FalI AAGNNNNNCTT 6 cut(s) 533, 565, 1344, 1376, 1590, 1622
FaqI GGGAC 2 cut(s) 1203, 1251
FauNDI CATATG 1 cut(s) 435
FbaI TGATCA 2 cut(s) 649, 1213
FblI GTMKAC 2 cut(s) 1300, 1366
Fnu4HI GCNGC 3 cut(s) 20, 246, 1266
FokI GGATG 8 cut(s) 12, 588, 749, 1050, 1130, 1164, 1325, 1642
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 3 cut(s) 20, 246, 1266
FspBI CTAG 1 cut(s) 1283
GlaI GCGC 1 cut(s) 1259
GluI GCNGC 3 cut(s) 20, 246, 1266
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 3 cut(s) 5, 813, 1406
HapII CCGG 2 cut(s) 305, 451
HhaI GCGC 1 cut(s) 1260
Hin6I GCGC 1 cut(s) 1258
HinP1I GCGC 1 cut(s) 1258
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 8 cut(s) 194, 226, 268, 301, 790, 977, 995, 1517
HpaII CCGG 2 cut(s) 305, 451
HphI GGTGA 4 cut(s) 794, 1208, 1210, 1223
Hpy166II GTNNAC 3 cut(s) 802, 1301, 1367
Hpy188I TCNGA 6 cut(s) 129, 838, 885, 1060, 1395, 1487
Hpy8I GTNNAC 3 cut(s) 802, 1301, 1367
Hpy99I CGWCG 1 cut(s) 594
HpyAV CCTTC 7 cut(s) 302, 601, 1084, 1105, 1178, 1180, 1642
HpyCH4III ACNGT 5 cut(s) 163, 344, 510, 1381, 1416
HpyCH4IV ACGT 2 cut(s) 1017, 1297
HpyCH4V TGCA 9 cut(s) 385, 468, 713, 1085, 1372, 1376, 1437, 1550, 1613
HpyF10VI GCNNNNNNNGC 1 cut(s) 1382
HpyF3I CTNAG 8 cut(s) 332, 426, 599, 741, 893, 1059, 1179, 1232
HpySE526I ACGT 2 cut(s) 1017, 1297
HspAI GCGC 1 cut(s) 1258
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 2 cut(s) 649, 1213
Kzo9I GATC 5 cut(s) 418, 484, 649, 838, 1213
LguI GCTCTTC 1 cut(s) 43
LmnI GCTCC 6 cut(s) 110, 137, 430, 1327, 1423, 1487
Lsp1109I GCAGC 2 cut(s) 6, 1252
MaeI CTAG 1 cut(s) 1283
MaeII ACGT 2 cut(s) 1017, 1297
MaeIII GTNAC 3 cut(s) 199, 873, 1410
MalI GATC 5 cut(s) 420, 486, 651, 840, 1215
MboI GATC 5 cut(s) 418, 484, 649, 838, 1213
MboII GAAGA 7 cut(s) 30, 307, 676, 898, 1118, 1298, 1474
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 115
MlyI GAGTC 1 cut(s) 989
MmeI TCCRAC 3 cut(s) 318, 951, 1398
MroI TCCGGA 1 cut(s) 304
MroXI GAANNNNTTC 3 cut(s) 832, 914, 1163
MseI TTAA 4 cut(s) 477, 858, 1626, 1675
MslI CAYNNNNRTG 3 cut(s) 380, 1112, 1545
MspA1I CMGCKG 1 cut(s) 871
MspI CCGG 2 cut(s) 305, 451
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 963
MvnI CGCG 1 cut(s) 1323
MwoI GCNNNNNNNGC 1 cut(s) 1382
NdeI CATATG 1 cut(s) 435
NdeII GATC 5 cut(s) 418, 484, 649, 838, 1213
NlaIV GGNNCC 4 cut(s) 139, 1191, 1239, 1489
NmeAIII GCCGAG 1 cut(s) 371
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 3 cut(s) 705, 800, 1376
OliI CACNNNNGTG 1 cut(s) 380
PaeI GCATGC 2 cut(s) 705, 1376
PagI TCATGA 1 cut(s) 1107
PceI AGGCCT 1 cut(s) 813
PciSI GCTCTTC 1 cut(s) 43
PctI GAATGC 1 cut(s) 963
PdmI GAANNNNTTC 3 cut(s) 832, 914, 1163
PfeI GAWTC 7 cut(s) 194, 226, 268, 301, 790, 977, 1517
PkrI GCNGC 3 cut(s) 21, 247, 1267
PleI GAGTC 1 cut(s) 989
PpsI GAGTC 1 cut(s) 989
Ppu21I YACGTR 1 cut(s) 1298
Psp124BI GAGCTC 1 cut(s) 115
PspN4I GGNNCC 4 cut(s) 139, 1191, 1239, 1489
PspPI GGNCC 2 cut(s) 1190, 1238
PvuII CAGCTG 1 cut(s) 871
RsaI GTAC 2 cut(s) 482, 723
RsaNI GTAC 2 cut(s) 481, 722
RseI CAYNNNNRTG 3 cut(s) 380, 1112, 1545
SacI GAGCTC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 43
SaqAI TTAA 4 cut(s) 477, 858, 1626, 1675
SatI GCNGC 3 cut(s) 20, 246, 1266
Sau3AI GATC 5 cut(s) 418, 484, 649, 838, 1213
Sau96I GGNCC 2 cut(s) 1190, 1238
SchI GAGTC 1 cut(s) 989
SduI GDGCHC 1 cut(s) 115
SinI GGWCC 2 cut(s) 1190, 1238
SmiMI CAYNNNNRTG 3 cut(s) 380, 1112, 1545
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SnaBI TACGTA 1 cut(s) 1298
SphI GCATGC 2 cut(s) 705, 1376
SseBI AGGCCT 1 cut(s) 813
SsiI CCGC 3 cut(s) 246, 272, 1321
SspI AATATT 1 cut(s) 728
SspMI CTAG 1 cut(s) 1283
SstI GAGCTC 1 cut(s) 115
StuI AGGCCT 1 cut(s) 813
TaaI ACNGT 5 cut(s) 163, 344, 510, 1381, 1416
TaiI ACGT 2 cut(s) 1020, 1300
TaqI TCGA 2 cut(s) 84, 592
TaqII GACCGA 1 cut(s) 632
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 7 cut(s) 194, 226, 268, 301, 790, 977, 1517
Tru1I TTAA 4 cut(s) 477, 858, 1626, 1675
Tru9I TTAA 4 cut(s) 477, 858, 1626, 1675
TscAI CASTG 1 cut(s) 1267
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 19, 1265
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 3 cut(s) 1061, 1164, 1517
TspRI CASTG 1 cut(s) 1267
VpaK11BI GGWCC 2 cut(s) 1190, 1238
XapI RAATTY 8 cut(s) 170, 236, 473, 749, 828, 1137, 1337, 1576
XceI RCATGY 3 cut(s) 705, 800, 1376
XcmI CCANNNNNNNNNTGG 3 cut(s) 1358, 1414, 1599
XmiI GTMKAC 2 cut(s) 1300, 1366
XmnI GAANNNNTTC 3 cut(s) 832, 914, 1163
XspI CTAG 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.