Rh4BG122900

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006733
Physical Location & Seq
Forward (+)
20603345 .. 20613387
10043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG122900.1

Sequence Viewer

Length: 1620 bp
ATGGCCTTGTTTCGCAGGCTGCTCCCTCTCTCCTCTTCCATCAACAGGACGAGCTTTTGCTCATCTGGAGCTCTACAAGGTTTTCGGAGTTATGGAGCCATTGCTACACCCGAGCCAACCGTGTTGAAATTTGAGAATGGAGAGACTGCTGATGATGAGATAGCAAAGATTCGGCGGGAATTTGATGCCGCAAAGCAGAGTTTCCTCAAGATTCCACAGGCACTTAAAGAATTGCCGAAGATGAATCCAGAAGGGATATATGTAAATAAAAACCTGAGGTTGGACAGTATTCAAGTTTATGGATTTGACTATGACTACACGCTGGCGCATTACTCGGCAGATTTACAGAAATTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGTTCCGGTATCCTGATGTTTGCATGGAATTTAAGTACGATCCTACATTTCCCATTAGAGGACTGTGCTTTGACAAATTGAAAGGGTGTCTCTTGAAGCTGGACTTTTTTGGGTCAATTGAGCCAGATGGATGCTACTATGGTCGTCGAAAGCTTAGTAGGAAGGAAATAGATGAGATATATGGCACAAGGCGTATCGGTCGTGATCAAGCAGAAAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGTGAGGCATGCCTGATTGCAGACATCGTACAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTAAATCGTGCGATTCAGCATGTTCACCGTACGGGCCTTGTTCATAGAGGAATTCTTTCTGATCCCCGTAAATATCTTGTTAAAAATGACCAGCTGTTACACTTTCTGAAGATGCTCAGGGAAAAAGGAAAGAAACTTTTCTTGCTGACTAACTCTCCATATTACTTTGTGGATGGAGGAATGCGTTTTTTGTTGGAGGATTCTTTGGGTTGTAATGACTCTTGGAGGGAACTTTTTGACGTTGTGATTGCCAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTGTTATGATGCAGAGAAGGATAATTTAGCTTTCATGAAGGTGGATGCGTTCCTTCCTAATAAAATTTATTACCACGGATGCCTGAAATCATTCCTTCAAATTACTAAGTGGAAGGGACCAGAGGTGATATACTTTGGTGATCACCTCTTTAGCGACCTAAGGGGACCATCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAAGATGAAATACGTATACAAAATGAGGATGCTTACCGCGTGGAGCAGGCAAAATTTCATATATTCCAAGAATTGCTTGGTAGACTGCATGCAACGGTAGCTAATAGTCAGAAAAGTGAGGACAAGTTACTGTTGGAGGAGCTAAATGAGTGCAGGCGTAAGGCAAGCTACAAGATGAAGAGAATGTTCAATAGCTCTTTCGGAGCCACCTTCCTTACGGATAAAGGTCAAGAATCTGCTTTTGCGTATCATATTCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTTCATGCACCCTACGATATTAAGATTATGCCACATCATGTGAAGGTTTCATCCATCTTGTTCAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

539

Amino Acids

62.65

Weight (kDa)

7.24

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 86 - 530 1.5e-149 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1243, 1309
AccII CGCG 1 cut(s) 1266
AciI CCGC 3 cut(s) 175, 189, 1264
AclWI GGATC 2 cut(s) 422, 776
AcsI RAATTY 8 cut(s) 128, 179, 416, 692, 771, 1080, 1280, 1519
AcuI CTGAAG 2 cut(s) 848, 1560
AfaI GTAC 3 cut(s) 425, 666, 751
AfiI CCNNNNNNNGG 3 cut(s) 45, 280, 446
AgsI TTSAA 7 cut(s) 127, 293, 469, 484, 1115, 1417, 1612
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 137, 482
AlwI GGATC 2 cut(s) 422, 776
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 2 cut(s) 3, 754
ApeKI GCWGC 2 cut(s) 19, 1208
ApoI RAATTY 8 cut(s) 128, 179, 416, 692, 771, 1080, 1280, 1519
Asp700I GAANNNNTTC 3 cut(s) 775, 857, 1106
AspLEI GCGC 2 cut(s) 328, 1203
AspS9I GGNCC 3 cut(s) 754, 1133, 1181
AsuHPI GGTGA 4 cut(s) 737, 1151, 1153, 1166
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 2 cut(s) 1133, 1181
AxyI CCTNAGG 2 cut(s) 275, 1175
BanII GRGCYC 1 cut(s) 73
BarI GAAGNNNNNNTAC 2 cut(s) 1221, 1253
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 6, 1195
BccI CCATC 6 cut(s) 47, 509, 613, 887, 1192, 1610
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 2 cut(s) 592, 1156
BcoDI GTCTC 2 cut(s) 137, 482
BfaI CTAG 1 cut(s) 1226
BfuI GTATCC 1 cut(s) 408
BisI GCNGC 3 cut(s) 20, 189, 1209
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 3 cut(s) 21, 190, 1210
Bme18I GGWCC 2 cut(s) 1133, 1181
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 754, 1133, 1181
BmiI GGNNCC 4 cut(s) 97, 1134, 1182, 1432
BpmI CTGGAG 1 cut(s) 87
Bpu10I CCTNAGC 2 cut(s) 369, 836
Bpu1102I GCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 1241
BsaBI GATNNNNATC 1 cut(s) 723
BsaJI CCNNGG 1 cut(s) 1090
BsaWI WCCGGW 1 cut(s) 393
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 280, 446
Bse1I ACTGG 1 cut(s) 1506
Bse21I CCTNAGG 2 cut(s) 275, 1175
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 1090
BseGI GGATG 8 cut(s) 524, 685, 898, 1006, 1066, 1100, 1261, 1598
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 45, 280, 446
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 266, 850, 993
BseNI ACTGG 1 cut(s) 1506
BseRI GAGGAG 2 cut(s) 22, 1379
BseXI GCAGC 2 cut(s) 6, 1195
BsgI GTGCAG 1 cut(s) 1399
Bsh1236I CGCG 1 cut(s) 1266
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 2 cut(s) 5, 756
BsiEI CGRYCG 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 394
BsiWI CGTACG 1 cut(s) 749
BslFI GGGAC 2 cut(s) 1146, 1194
BslI CCNNNNNNNGG 3 cut(s) 45, 280, 446
BsmAI GTCTC 2 cut(s) 137, 482
BsmFI GGGAC 2 cut(s) 1146, 1194
BsmI GAATGC 1 cut(s) 906
BsnI GGCC 2 cut(s) 5, 756
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 5 cut(s) 361, 427, 592, 781, 1156
Bsp1720I GCTNAGC 1 cut(s) 684
BspACI CCGC 3 cut(s) 175, 189, 1264
BspANI GGCC 2 cut(s) 5, 756
BspCNI CTCAG 3 cut(s) 267, 849, 994
BspFNI CGCG 1 cut(s) 1266
BspHI TCATGA 1 cut(s) 1050
BspLI GGNNCC 4 cut(s) 97, 1134, 1182, 1432
BspPI GGATC 2 cut(s) 422, 776
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 1 cut(s) 1506
BssECI CCNNGG 1 cut(s) 1090
BssMI GATC 5 cut(s) 361, 427, 592, 781, 1156
BssNAI GTATAC 1 cut(s) 1244
Bst1107I GTATAC 1 cut(s) 1244
Bst4CI ACNGT 6 cut(s) 121, 287, 453, 749, 1324, 1359
Bst6I CTCTTC 2 cut(s) 40, 1400
BstBAI YACGTR 1 cut(s) 1241
BstC8I GCNNGC 8 cut(s) 17, 324, 646, 703, 1275, 1317, 1382, 1393
BstDEI CTNAG 8 cut(s) 275, 369, 542, 684, 836, 1002, 1122, 1175
BstDSI CCRYGG 1 cut(s) 1090
BstF5I GGATG 8 cut(s) 524, 685, 898, 1006, 1066, 1100, 1261, 1598
BstFNI CGCG 1 cut(s) 1266
BstHHI GCGC 2 cut(s) 328, 1203
BstKTI GATC 5 cut(s) 364, 430, 595, 784, 1159
BstMAI GTCTC 2 cut(s) 137, 482
BstMBI GATC 5 cut(s) 361, 427, 592, 781, 1156
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 1 cut(s) 1325
BstNSI RCATGY 3 cut(s) 648, 743, 1319
BstSNI TACGTA 1 cut(s) 1241
BstUI CGCG 1 cut(s) 1266
BstV1I GCAGC 2 cut(s) 6, 1195
BstZ17I GTATAC 1 cut(s) 1244
Bsu36I CCTNAGG 2 cut(s) 275, 1175
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 5, 756
BtgI CCRYGG 1 cut(s) 1090
BtsCI GGATG 8 cut(s) 524, 685, 898, 1006, 1066, 1100, 1261, 1598
BtsI GCAGTG 1 cut(s) 1203
BtsIMutI CAGTG 1 cut(s) 1203
Cac8I GCNNGC 8 cut(s) 17, 324, 646, 703, 1275, 1317, 1382, 1393
CciI TCATGA 1 cut(s) 1050
CfoI GCGC 2 cut(s) 328, 1203
Cfr13I GGNCC 3 cut(s) 754, 1133, 1181
Csp6I GTAC 3 cut(s) 424, 665, 750
CviAII CATG 9 cut(s) 412, 645, 740, 1051, 1220, 1316, 1545, 1553, 1586
CviQI GTAC 3 cut(s) 424, 665, 750
DdeI CTNAG 8 cut(s) 275, 369, 542, 684, 836, 1002, 1122, 1175
DpnI GATC 5 cut(s) 363, 429, 594, 783, 1158
DpnII GATC 5 cut(s) 361, 427, 592, 781, 1156
Eam1104I CTCTTC 2 cut(s) 40, 1400
EarI CTCTTC 2 cut(s) 40, 1400
Ecl136II GAGCTC 1 cut(s) 71
Eco105I TACGTA 1 cut(s) 1241
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 1133, 1181
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 848, 1560
Eco81I CCTNAGG 2 cut(s) 275, 1175
Eco88I CYCGRG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 71
EcoRI GAATTC 1 cut(s) 771
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 9 cut(s) 415, 648, 743, 1054, 1223, 1319, 1548, 1556, 1589
FalI AAGNNNNNCTT 6 cut(s) 476, 508, 1287, 1319, 1533, 1565
FaqI GGGAC 2 cut(s) 1146, 1194
FatI CATG 9 cut(s) 411, 644, 739, 1050, 1219, 1315, 1544, 1552, 1585
FauI CCCGC 1 cut(s) 168
FauNDI CATATG 1 cut(s) 378
FbaI TGATCA 2 cut(s) 592, 1156
FblI GTMKAC 2 cut(s) 1243, 1309
Fnu4HI GCNGC 3 cut(s) 20, 189, 1209
FokI GGATG 8 cut(s) 531, 692, 905, 993, 1073, 1107, 1268, 1585
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 3 cut(s) 20, 189, 1209
FspBI CTAG 1 cut(s) 1226
GlaI GCGC 2 cut(s) 327, 1202
GluI GCNGC 3 cut(s) 20, 189, 1209
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 2 cut(s) 5, 756
HapII CCGG 1 cut(s) 394
HhaI GCGC 2 cut(s) 328, 1203
Hin1II CATG 9 cut(s) 415, 648, 743, 1054, 1223, 1319, 1548, 1556, 1589
Hin6I GCGC 2 cut(s) 326, 1201
HinP1I GCGC 2 cut(s) 326, 1201
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 7 cut(s) 169, 211, 244, 733, 920, 938, 1460
HpaII CCGG 1 cut(s) 394
HphI GGTGA 4 cut(s) 737, 1151, 1153, 1166
Hpy166II GTNNAC 3 cut(s) 745, 1244, 1310
Hpy188I TCNGA 6 cut(s) 87, 781, 828, 1003, 1338, 1430
Hpy8I GTNNAC 3 cut(s) 745, 1244, 1310
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 9 cut(s) 245, 544, 1027, 1048, 1079, 1121, 1123, 1447, 1585
HpyCH4III ACNGT 6 cut(s) 121, 287, 453, 749, 1324, 1359
HpyCH4IV ACGT 2 cut(s) 960, 1240
HpyCH4V TGCA 8 cut(s) 411, 656, 1028, 1315, 1319, 1380, 1493, 1556
HpyF10VI GCNNNNNNNGC 1 cut(s) 1325
HpyF3I CTNAG 8 cut(s) 275, 369, 542, 684, 836, 1002, 1122, 1175
HpySE526I ACGT 2 cut(s) 960, 1240
Hsp92II CATG 9 cut(s) 415, 648, 743, 1054, 1223, 1319, 1548, 1556, 1589
HspAI GCGC 2 cut(s) 326, 1201
Ksp22I TGATCA 2 cut(s) 592, 1156
Kzo9I GATC 5 cut(s) 361, 427, 592, 781, 1156
LmnI GCTCC 7 cut(s) 27, 68, 95, 373, 1270, 1366, 1430
Lsp1109I GCAGC 2 cut(s) 6, 1195
MaeI CTAG 1 cut(s) 1226
MaeII ACGT 2 cut(s) 960, 1240
MaeIII GTNAC 2 cut(s) 816, 1353
MalI GATC 5 cut(s) 363, 429, 594, 783, 1158
MboI GATC 5 cut(s) 361, 427, 592, 781, 1156
MboII GAAGA 6 cut(s) 27, 250, 619, 841, 1241, 1417
MfeI CAATTG 1 cut(s) 504
MhlI GDGCHC 1 cut(s) 73
MlyI GAGTC 1 cut(s) 932
MmeI TCCRAC 3 cut(s) 261, 894, 1341
MroXI GAANNNNTTC 3 cut(s) 775, 857, 1106
MseI TTAA 4 cut(s) 225, 420, 801, 1569
MslI CAYNNNNRTG 2 cut(s) 1055, 1488
MspA1I CMGCKG 1 cut(s) 814
MspI CCGG 1 cut(s) 394
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 906
MvnI CGCG 1 cut(s) 1266
MwoI GCNNNNNNNGC 1 cut(s) 1325
NdeI CATATG 1 cut(s) 378
NdeII GATC 5 cut(s) 361, 427, 592, 781, 1156
NlaIII CATG 9 cut(s) 415, 648, 743, 1054, 1223, 1319, 1548, 1556, 1589
NlaIV GGNNCC 4 cut(s) 97, 1134, 1182, 1432
NmeAIII GCCGAG 1 cut(s) 314
NspI RCATGY 3 cut(s) 648, 743, 1319
PaeI GCATGC 2 cut(s) 648, 1319
PagI TCATGA 1 cut(s) 1050
PctI GAATGC 1 cut(s) 906
PdmI GAANNNNTTC 3 cut(s) 775, 857, 1106
PfeI GAWTC 6 cut(s) 169, 211, 244, 733, 920, 1460
Pfl23II CGTACG 1 cut(s) 749
PkrI GCNGC 3 cut(s) 21, 190, 1210
PleI GAGTC 1 cut(s) 932
PpsI GAGTC 1 cut(s) 932
Ppu21I YACGTR 1 cut(s) 1241
Psp124BI GAGCTC 1 cut(s) 73
PspLI CGTACG 1 cut(s) 749
PspN4I GGNNCC 4 cut(s) 97, 1134, 1182, 1432
PspPI GGNCC 3 cut(s) 754, 1133, 1181
PvuII CAGCTG 1 cut(s) 814
RsaI GTAC 3 cut(s) 425, 666, 751
RsaNI GTAC 3 cut(s) 424, 665, 750
RseI CAYNNNNRTG 2 cut(s) 1055, 1488
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 4 cut(s) 225, 420, 801, 1569
SatI GCNGC 3 cut(s) 20, 189, 1209
Sau3AI GATC 5 cut(s) 361, 427, 592, 781, 1156
Sau96I GGNCC 3 cut(s) 754, 1133, 1181
SchI GAGTC 1 cut(s) 932
SduI GDGCHC 1 cut(s) 73
SinI GGWCC 2 cut(s) 1133, 1181
SmiMI CAYNNNNRTG 2 cut(s) 1055, 1488
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SnaBI TACGTA 1 cut(s) 1241
SphI GCATGC 2 cut(s) 648, 1319
SsiI CCGC 3 cut(s) 175, 189, 1264
SspI AATATT 1 cut(s) 671
SspMI CTAG 1 cut(s) 1226
SstI GAGCTC 1 cut(s) 73
TaaI ACNGT 6 cut(s) 121, 287, 453, 749, 1324, 1359
TaiI ACGT 2 cut(s) 963, 1243
TaqI TCGA 1 cut(s) 535
TaqII GACCGA 1 cut(s) 575
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 6 cut(s) 169, 211, 244, 733, 920, 1460
Tru1I TTAA 4 cut(s) 225, 420, 801, 1569
Tru9I TTAA 4 cut(s) 225, 420, 801, 1569
TscAI CASTG 1 cut(s) 1210
TseI GCWGC 2 cut(s) 19, 1208
TspGWI ACGGA 3 cut(s) 1004, 1107, 1460
TspRI CASTG 1 cut(s) 1210
VpaK11BI GGWCC 2 cut(s) 1133, 1181
XapI RAATTY 8 cut(s) 128, 179, 416, 692, 771, 1080, 1280, 1519
XceI RCATGY 3 cut(s) 648, 743, 1319
XcmI CCANNNNNNNNNTGG 2 cut(s) 1301, 1542
XmiI GTMKAC 2 cut(s) 1243, 1309
XmnI GAANNNNTTC 3 cut(s) 775, 857, 1106
XspI CTAG 1 cut(s) 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.