Rroxscaffold_5G00349390

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006733
Physical Location & Seq
Forward (+)
22326139 .. 22336443
10305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00349390.1

Sequence Viewer

Length: 1653 bp
ATGAGTTGCACTATGACTTGTAATGTTTACTGTAAATGTGTTTGTATATTGATCATTACGAATGAATGGAGCTGTTCAATTTACTTAAATTTAGGTTTTCGGAGTTATGGAGCCATTGCTACACCCGAGCCAACCGTGTTGAAATTTGAGAATGGAGAGACTGCTGATTCTGTGACTGCTGATGAGGAGATAGCAAAGATTCGGCGTGAATTTGATGCCGCAAAGCAGAGTTTCCTCAAGATTCCACAGGCACTTAAAGAAGTGCCGAAGATGAATCCGGAAGGGATATATGTAAATAAAAACCTGAGGTTGGACAGTATTCAAGTTTATGGATTTGACTATGACTACACGCTGGCCCATTACTCGGCAAATTTACAGAACTTGATATATGATCTTGCTAAGGAGCATATGGTCAATGAGTTCCGGTATCCTGAGGTTTGCATGGAATTTAAGTACGATCCTACTTTTCCCGTTAGAGGACTGTGCTATGACAAATTGAAAGGGTGTCTCTTGAAGCTGGACTTTTTTGGGTCAATTGAGCCGGATGGATGCTACTATGGTCGTAGAAAGCTTAGTAGGAAGGAAATAGATGAGATATATGGCACAAGGCGGATTGGTCGTGATCAAGCAGGAAGCCTTGTTGGGTTGATGGATTTCTTCTGCTTTAGTGAGGCATGCCTCATTGCAGACATCGTACAATATTTTGTGGATGCTAAGCTGGAATTTGATGCTTGCTACATCTATCAAGATGTAAATCGTGCGATTCAGCATGTTCATCGTACTGGCCTTGTTCATAGAGGAGTTCTTTCTGATCCCCATAAATATCTTGTTAAAAATGGCCAGCTGTTACACTTTCTGAAGATGCTCAAGGAAAAAGGAAAGAAACTATTCTTGCTAACTAACTCTCCATATTACTTTGTGGATGGAGGAATGCGTTTTTTGTTGGAGGATTCTTTGGGTTGTAATGACTCTTGGAGGGAACTTTTTGACGTTGTGATTGCCAAAGCCAATAAGCCAGATTTCTACACATCTGAGCATCCATTCCGTTGTTATGATGCAGAGAAGGATAATTTAACTTTCACGAAGGTGGATGCGTTTCTTCCTAATAAAATTTATTACCATGGATGCCTGAAATCATTCCTTCAAATTACCAAGTGGAAGGGACCAGAGGTGATTTACTTTGGTGATCACCTCTTTAGCGACCTAAGGGGACCATCAAAAGCTGGTTGGCGCACTGCTGCTATAATCCATGAACTAGAAGATGAAATACTTACACAAAATGAGGATGCTTACCGCGTGGAGCAGGCAAAATTTCATATATTGCAAGAATTGCTTGGTAGACTGCATGCAACGGTAGCTAATAGTCAGAAAAGTGAGGCCAAGTTACTGTTGGACGAGCTAAATGAGTGCAGGCGTAAGGCAAGCTACAAGATGAAGAGAATGTTCAATAGCTCTTTCGGAGCCACCTTCCTTACGGATAAAGGTCAAGAATCTGCTTTCGCGTATCATATTCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTTCATGCACCCTTCGATATTAAAATTATGCCACATCATGTGAAGACGCTATGCAAGTCTAGATATGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

550

Amino Acids

63.71

Weight (kDa)

6.81

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 96 - 540 1.6e-149 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1339
AccII CGCG 2 cut(s) 1296, 1502
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 3 cut(s) 219, 610, 1294
AclWI GGATC 2 cut(s) 452, 806
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 9 cut(s) 88, 143, 209, 370, 446, 722, 1110, 1310, 1549
AcuI CTGAAG 2 cut(s) 878, 1590
AfaI GTAC 3 cut(s) 455, 696, 781
AfiI CCNNNNNNNGG 3 cut(s) 310, 364, 476
AgsI TTSAA 7 cut(s) 78, 142, 323, 499, 514, 1145, 1447
AleI CACNNNNGTG 1 cut(s) 1085
Alw26I GTCTC 2 cut(s) 152, 512
AlwI GGATC 2 cut(s) 452, 806
Ama87I CYCGRG 1 cut(s) 125
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 4 cut(s) 354, 784, 838, 1377
ApeKI GCWGC 1 cut(s) 1238
ApoI RAATTY 9 cut(s) 88, 143, 209, 370, 446, 722, 1110, 1310, 1549
Asp700I GAANNNNTTC 2 cut(s) 887, 1136
AspLEI GCGC 1 cut(s) 1233
AspS9I GGNCC 3 cut(s) 355, 1163, 1211
AsuHPI GGTGA 3 cut(s) 1181, 1183, 1196
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 2 cut(s) 1163, 1211
AxyI CCTNAGG 3 cut(s) 305, 432, 1205
BalI TGGCCA 1 cut(s) 840
BarI GAAGNNNNNNTAC 2 cut(s) 1251, 1283
BbsI GAAGAC 1 cut(s) 1628
BbvI GCAGC 1 cut(s) 1225
BccI CCATC 4 cut(s) 539, 643, 917, 1222
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciVI GTATCC 1 cut(s) 438
BclI TGATCA 3 cut(s) 51, 622, 1186
BcoDI GTCTC 2 cut(s) 152, 512
BfaI CTAG 2 cut(s) 1256, 1638
BfuI GTATCC 1 cut(s) 438
BisI GCNGC 2 cut(s) 219, 1239
BlpI GCTNAGC 1 cut(s) 714
BlsI GCNGC 2 cut(s) 220, 1240
Bme18I GGWCC 2 cut(s) 1163, 1211
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 3 cut(s) 355, 1163, 1211
BmiI GGNNCC 4 cut(s) 112, 1164, 1212, 1462
BpiI GAAGAC 1 cut(s) 1628
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 714
BpuEI CTTGAG 2 cut(s) 221, 851
BsaBI GATNNNNATC 1 cut(s) 753
BsaJI CCNNGG 1 cut(s) 1120
BsaWI WCCGGW 2 cut(s) 277, 423
BsaXI ACNNNNNCTCC 2 cut(s) 889, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 364, 476
Bse1I ACTGG 2 cut(s) 787, 1536
Bse21I CCTNAGG 3 cut(s) 305, 432, 1205
Bse3DI GCAATG 2 cut(s) 114, 681
Bse8I GATNNNNATC 1 cut(s) 753
BseAI TCCGGA 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 1120
BseGI GGATG 8 cut(s) 550, 554, 715, 928, 1036, 1096, 1130, 1291
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 3 cut(s) 310, 364, 476
BseMI GCAATG 2 cut(s) 114, 681
BseMII CTCAG 3 cut(s) 296, 423, 1023
BseNI ACTGG 2 cut(s) 787, 1536
BseRI GAGGAG 2 cut(s) 200, 813
BseXI GCAGC 1 cut(s) 1225
BsgI GTGCAG 1 cut(s) 1429
Bsh1236I CGCG 2 cut(s) 1296, 1502
BshFI GGCC 4 cut(s) 356, 786, 840, 1379
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 3 cut(s) 278, 424, 542
BslFI GGGAC 2 cut(s) 1176, 1224
BslI CCNNNNNNNGG 3 cut(s) 310, 364, 476
BsmAI GTCTC 2 cut(s) 152, 512
BsmFI GGGAC 2 cut(s) 1176, 1224
BsmI GAATGC 1 cut(s) 936
BsnI GGCC 4 cut(s) 356, 786, 840, 1379
BsoBI CYCGRG 1 cut(s) 125
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
Bsp1720I GCTNAGC 1 cut(s) 714
Bsp19I CCATGG 1 cut(s) 1120
BspACI CCGC 3 cut(s) 219, 610, 1294
BspANI GGCC 4 cut(s) 356, 786, 840, 1379
BspCNI CTCAG 3 cut(s) 297, 424, 1024
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 2 cut(s) 1296, 1502
BspLI GGNNCC 4 cut(s) 112, 1164, 1212, 1462
BspPI GGATC 2 cut(s) 452, 806
BsrDI GCAATG 2 cut(s) 114, 681
BsrI ACTGG 2 cut(s) 787, 1536
BssECI CCNNGG 1 cut(s) 1120
BssMI GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
BssT1I CCWWGG 1 cut(s) 1120
Bst4CI ACNGT 6 cut(s) 32, 136, 317, 483, 1354, 1389
Bst6I CTCTTC 1 cut(s) 1430
BstAPI GCANNNNNTGC 1 cut(s) 1330
BstC8I GCNNGC 8 cut(s) 354, 676, 733, 842, 1305, 1347, 1412, 1423
BstDEI CTNAG 7 cut(s) 305, 399, 432, 572, 714, 1032, 1205
BstDSI CCRYGG 1 cut(s) 1120
BstF5I GGATG 8 cut(s) 550, 554, 715, 928, 1036, 1096, 1130, 1291
BstFNI CGCG 2 cut(s) 1296, 1502
BstHHI GCGC 1 cut(s) 1233
BstKTI GATC 6 cut(s) 54, 394, 460, 625, 814, 1189
BstMAI GTCTC 2 cut(s) 152, 512
BstMBI GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 1330, 1355
BstNSI RCATGY 3 cut(s) 678, 773, 1349
BstUI CGCG 2 cut(s) 1296, 1502
BstV1I GCAGC 1 cut(s) 1225
BstV2I GAAGAC 1 cut(s) 1628
Bsu36I CCTNAGG 3 cut(s) 305, 432, 1205
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 4 cut(s) 356, 786, 840, 1379
BtgI CCRYGG 1 cut(s) 1120
BtsCI GGATG 8 cut(s) 550, 554, 715, 928, 1036, 1096, 1130, 1291
BtsI GCAGTG 1 cut(s) 1233
BtsIMutI CAGTG 1 cut(s) 1233
Cac8I GCNNGC 8 cut(s) 354, 676, 733, 842, 1305, 1347, 1412, 1423
CfoI GCGC 1 cut(s) 1233
Cfr13I GGNCC 3 cut(s) 355, 1163, 1211
CseI GACGC 1 cut(s) 1633
Csp6I GTAC 3 cut(s) 454, 695, 780
CviAII CATG 9 cut(s) 442, 675, 770, 1121, 1250, 1346, 1575, 1583, 1616
CviQI GTAC 3 cut(s) 454, 695, 780
DdeI CTNAG 7 cut(s) 305, 399, 432, 572, 714, 1032, 1205
DpnI GATC 6 cut(s) 53, 393, 459, 624, 813, 1188
DpnII GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
EaeI YGGCCR 1 cut(s) 838
Eam1104I CTCTTC 1 cut(s) 1430
EarI CTCTTC 1 cut(s) 1430
EciI GGCGGA 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 1120
Eco47I GGWCC 2 cut(s) 1163, 1211
Eco57I CTGAAG 2 cut(s) 878, 1590
Eco81I CCTNAGG 3 cut(s) 305, 432, 1205
Eco88I CYCGRG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 1120
ErhI CCWWGG 1 cut(s) 1120
FaeI CATG 9 cut(s) 445, 678, 773, 1124, 1253, 1349, 1578, 1586, 1619
FalI AAGNNNNNCTT 6 cut(s) 506, 538, 1317, 1349, 1563, 1595
FaqI GGGAC 2 cut(s) 1176, 1224
FatI CATG 9 cut(s) 441, 674, 769, 1120, 1249, 1345, 1574, 1582, 1615
FauNDI CATATG 1 cut(s) 408
FbaI TGATCA 3 cut(s) 51, 622, 1186
FblI GTMKAC 1 cut(s) 1339
Fnu4HI GCNGC 2 cut(s) 219, 1239
FokI GGATG 8 cut(s) 557, 561, 722, 935, 1023, 1103, 1137, 1298
Fsp4HI GCNGC 2 cut(s) 219, 1239
FspBI CTAG 2 cut(s) 1256, 1638
GlaI GCGC 1 cut(s) 1232
GluI GCNGC 2 cut(s) 219, 1239
HaeIII GGCC 4 cut(s) 356, 786, 840, 1379
HapII CCGG 3 cut(s) 278, 424, 542
HgaI GACGC 1 cut(s) 1633
HhaI GCGC 1 cut(s) 1233
Hin1II CATG 9 cut(s) 445, 678, 773, 1124, 1253, 1349, 1578, 1586, 1619
Hin6I GCGC 1 cut(s) 1231
HinP1I GCGC 1 cut(s) 1231
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 8 cut(s) 167, 199, 241, 274, 763, 950, 968, 1490
HpaII CCGG 3 cut(s) 278, 424, 542
HphI GGTGA 3 cut(s) 1181, 1183, 1196
Hpy166II GTNNAC 2 cut(s) 28, 1340
Hpy188I TCNGA 6 cut(s) 102, 811, 858, 1033, 1368, 1460
Hpy188III TCNNGA 9 cut(s) 238, 278, 431, 511, 620, 746, 1081, 1487, 1638
Hpy8I GTNNAC 2 cut(s) 28, 1340
HpyAV CCTTC 8 cut(s) 275, 574, 1057, 1078, 1151, 1153, 1477, 1600
HpyCH4III ACNGT 6 cut(s) 32, 136, 317, 483, 1354, 1389
HpyCH4IV ACGT 1 cut(s) 990
HpyF10VI GCNNNNNNNGC 2 cut(s) 1330, 1355
HpyF3I CTNAG 7 cut(s) 305, 399, 432, 572, 714, 1032, 1205
HpySE526I ACGT 1 cut(s) 990
Hsp92II CATG 9 cut(s) 445, 678, 773, 1124, 1253, 1349, 1578, 1586, 1619
HspAI GCGC 1 cut(s) 1231
Kpn2I TCCGGA 1 cut(s) 277
Ksp22I TGATCA 3 cut(s) 51, 622, 1186
Kzo9I GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
LmnI GCTCC 5 cut(s) 69, 110, 403, 1300, 1460
Lsp1109I GCAGC 1 cut(s) 1225
MaeI CTAG 2 cut(s) 1256, 1638
MaeII ACGT 1 cut(s) 990
MaeIII GTNAC 3 cut(s) 172, 846, 1383
MalI GATC 6 cut(s) 53, 393, 459, 624, 813, 1188
MboI GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
MboII GAAGA 7 cut(s) 280, 649, 871, 1091, 1271, 1447, 1633
MfeI CAATTG 1 cut(s) 534
MlsI TGGCCA 1 cut(s) 840
MluNI TGGCCA 1 cut(s) 840
MlyI GAGTC 1 cut(s) 962
MmeI TCCRAC 3 cut(s) 291, 924, 1371
Mox20I TGGCCA 1 cut(s) 840
MroI TCCGGA 1 cut(s) 277
MroXI GAANNNNTTC 2 cut(s) 887, 1136
MscI TGGCCA 1 cut(s) 840
MseI TTAA 6 cut(s) 86, 255, 450, 831, 1073, 1599
MslI CAYNNNNRTG 2 cut(s) 1085, 1518
Msp20I TGGCCA 1 cut(s) 840
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 3 cut(s) 278, 424, 542
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 936
MvnI CGCG 2 cut(s) 1296, 1502
MwoI GCNNNNNNNGC 2 cut(s) 1330, 1355
NcoI CCATGG 1 cut(s) 1120
NdeI CATATG 1 cut(s) 408
NdeII GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
NlaIII CATG 9 cut(s) 445, 678, 773, 1124, 1253, 1349, 1578, 1586, 1619
NlaIV GGNNCC 4 cut(s) 112, 1164, 1212, 1462
NmeAIII GCCGAG 1 cut(s) 344
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 3 cut(s) 678, 773, 1349
OliI CACNNNNGTG 1 cut(s) 1085
PaeI GCATGC 2 cut(s) 678, 1349
PctI GAATGC 1 cut(s) 936
PdmI GAANNNNTTC 2 cut(s) 887, 1136
PfeI GAWTC 7 cut(s) 167, 199, 241, 274, 763, 950, 1490
PkrI GCNGC 2 cut(s) 220, 1240
PleI GAGTC 1 cut(s) 962
PpsI GAGTC 1 cut(s) 962
PspN4I GGNNCC 4 cut(s) 112, 1164, 1212, 1462
PspPI GGNCC 3 cut(s) 355, 1163, 1211
PvuII CAGCTG 1 cut(s) 844
RsaI GTAC 3 cut(s) 455, 696, 781
RsaNI GTAC 3 cut(s) 454, 695, 780
RseI CAYNNNNRTG 2 cut(s) 1085, 1518
SaqAI TTAA 6 cut(s) 86, 255, 450, 831, 1073, 1599
SatI GCNGC 2 cut(s) 219, 1239
Sau3AI GATC 6 cut(s) 51, 391, 457, 622, 811, 1186
Sau96I GGNCC 3 cut(s) 355, 1163, 1211
SchI GAGTC 1 cut(s) 962
SinI GGWCC 2 cut(s) 1163, 1211
SmiMI CAYNNNNRTG 2 cut(s) 1085, 1518
SmlI CTYRAG 2 cut(s) 236, 866
SmoI CTYRAG 2 cut(s) 236, 866
SphI GCATGC 2 cut(s) 678, 1349
SsiI CCGC 3 cut(s) 219, 610, 1294
SspI AATATT 1 cut(s) 701
SspMI CTAG 2 cut(s) 1256, 1638
StyI CCWWGG 1 cut(s) 1120
TaaI ACNGT 6 cut(s) 32, 136, 317, 483, 1354, 1389
TaiI ACGT 1 cut(s) 993
TaqI TCGA 1 cut(s) 1593
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 7 cut(s) 167, 199, 241, 274, 763, 950, 1490
Tru1I TTAA 6 cut(s) 86, 255, 450, 831, 1073, 1599
Tru9I TTAA 6 cut(s) 86, 255, 450, 831, 1073, 1599
TscAI CASTG 1 cut(s) 1240
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 1238
Tsp45I GTSAC 1 cut(s) 172
TspGWI ACGGA 2 cut(s) 1034, 1490
TspRI CASTG 1 cut(s) 1240
VpaK11BI GGWCC 2 cut(s) 1163, 1211
XapI RAATTY 9 cut(s) 88, 143, 209, 370, 446, 722, 1110, 1310, 1549
XbaI TCTAGA 1 cut(s) 1637
XceI RCATGY 3 cut(s) 678, 773, 1349
XcmI CCANNNNNNNNNTGG 2 cut(s) 1387, 1572
XmiI GTMKAC 1 cut(s) 1339
XmnI GAANNNNTTC 2 cut(s) 887, 1136
XspI CTAG 2 cut(s) 1256, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.