AT2G27350

OTU domain-containing protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003236
Physical Location & Seq
Reverse (-)
11699548 .. 11704303
4756 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27350.5

Sequence Viewer

Length: 1518 bp
ATGACTCGGATTTTGGTTCAAAGAGGTTCATCAGGCAGTTCTTCCAACTCTAGCCGTCCTTCTTCCTCATCTAGTTCTTCCTCGGGATCAGAAACACAGATAAACAATAATATTCCGGTTCCTCCTGTTACTATAGATGAAGAAATTACGGATGAGAAACAAGAAGAGGTTACTGTAGTTGAAAAAGCAGAGTGTTCTGATGCTAAAGATGTAGCTGTGGACAGTGATGAACCTGCGGATAGAGAAGATGATGAAGGGTTGGTAGTTGCAGAAAATGTTCATGTTCAAAGTGAAGGTATAGATTGTGATTCTCCAGTTAGCGGTGGCAGTAACTCTGATTCACCACCCGTACCAGCTCCACCACCAAAACCTTCTTCCACTGTCAATCCTGGTAGTAACAGATCGGTTTTGGGAAGTTTTGGCGCTTTACGAATTGGACCAACACGAAGAGCTGCTGGGCCTCGCTCTCTTGTTTCCAGTAGGAGTTCGCCAACTGGATCACATCCTTCTTCTCCAAGATCACACAGTGAGAACGAGGGCTATAATAGTTCTGATGAGCATATGCCATGCTATGTGCCCTCTCATCCTGGCTCTGGCTTGGAAAGAGAACACCAGTTTGAAGCAGAGATTAGATATTCAAAAGGATTTGAAATTAGGCGGATGTTGGAAGATGGAAATTGTCTTTTTCGAGCTGTTGCAGATCAAGTATATGGGGACTCAGAGGCATATGACTTGGCTAGACAAATGTGCATGGATTACATGGAACAAGAGAGGGATCACTTTTCTCAGTTCATAACCGAAGGTTTTACCTCTTACTTGAAGAGGAAAAGAAGAGATAAGGTCTATGGAAACAACGTAGAGATCCAAGCTTTAGCAGAAATGTACAATAGGCCAATCCACATTTACTCATATAGCACAGAGCCTATCAACATATTTCAAGGAAACTACAGCACGGATACACCTCCCATAAGGCTGAGTTACCACCACGGGAATCATTATAATTCTTTGGTGGATCCACATCGGTTGACAGTTGGTGCAGGACTTGGATTTAGTAGCCTTAGTGGGAGACACGTGGACAAGGAACAGGTGAAAGCTGCTATAAAGGCTCAGCAAGAACATCAAATTGATAATGCGCTGTTAGCAGAAGGGAGATTTTACTCTGACCTTGAGCTTACGGAAAAGGAAATAGAGCGGTCGGTAATGGAAGCTTCTCGTGCTGAGTATCTTATGGAATGGTCTAAGCCACGAATTGGTCCAAAGGAATCATCCACCTCGAATGCTGAGACATCATCTTCTGGAGCTAGACCTTCAGGCAGCGATTCGAAACCAGCAGAGGCAGTAAAAGAAAAGACGGTGCTGAGCAGCAGTATCGAGATGGTATTGTCGATGGGATTTAGCTACGCACAGGCCATGGAAGCTTATAGCATTTTTGGGGATGACGTTGACTCTATGGTCTGTTATGTACTGGAGACGAGCTGTGGCGGCAACAACCGACGCAAAGGCAAAGCCACGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 map04622
Pfam Domains
Protein Families
OTU

Protein Analysis

505

Amino Acids

55.27

Weight (kDa)

5.05

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 224 - 333 8.3e-18 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 999
AasI GACNNNNNNGTC 1 cut(s) 1451
Acc36I ACCTGC 1 cut(s) 241
AccB7I CCANNNNNTGG 1 cut(s) 1250
AccBSI CCGCTC 1 cut(s) 1192
AciI CCGC 5 cut(s) 236, 321, 658, 1192, 1482
AclWI GGATC 6 cut(s) 94, 505, 783, 856, 1007, 1020
AcuI CTGAAG 1 cut(s) 1293
AcvI CACGTG 1 cut(s) 1072
AdeI CACNNNGTG 1 cut(s) 527
AfaI GTAC 3 cut(s) 351, 884, 1464
AfiI CCNNNNNNNGG 3 cut(s) 320, 593, 1250
AflIII ACRYGT 1 cut(s) 1069
AgsI TTSAA 8 cut(s) 20, 182, 287, 620, 639, 650, 820, 938
AjnI CCWGG 2 cut(s) 388, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
Alw26I GTCTC 3 cut(s) 1060, 1277, 1463
AlwI GGATC 6 cut(s) 94, 505, 783, 856, 1007, 1020
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 3 cut(s) 458, 890, 1407
ApeKI GCWGC 4 cut(s) 452, 1094, 1314, 1362
ArsI GACNNNNNNTTYG 1 cut(s) 27
Asp700I GAANNNNTTC 1 cut(s) 276
AspLEI GCGC 2 cut(s) 425, 1135
AspS9I GGNCC 3 cut(s) 437, 458, 1253
AsuHPI GGTGA 2 cut(s) 333, 1099
AsuII TTCGAA 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 2 cut(s) 437, 1253
BaeGI GKGCMC 1 cut(s) 579
BamHI GGATCC 1 cut(s) 1012
BauI CACGAG 1 cut(s) 1212
BbrPI CACGTG 1 cut(s) 1072
BbvI GCAGC 4 cut(s) 439, 1081, 1326, 1374
BccI CCATC 3 cut(s) 665, 1369, 1381
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 2 cut(s) 1351, 1385
BciT130I CCWGG 2 cut(s) 390, 588
BciVI GTATCC 1 cut(s) 949
BcoDI GTCTC 3 cut(s) 1060, 1277, 1463
BfaI CTAG 4 cut(s) 51, 72, 738, 1302
BfmI CTRYAG 3 cut(s) 132, 174, 946
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 241
BfuI GTATCC 1 cut(s) 949
BisI GCNGC 5 cut(s) 453, 1095, 1315, 1363, 1483
BlpI GCTNAGC 2 cut(s) 1107, 1358
BlsI GCNGC 5 cut(s) 454, 1096, 1316, 1364, 1484
Bme1390I CCNGG 2 cut(s) 390, 588
Bme18I GGWCC 2 cut(s) 437, 1253
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 3 cut(s) 437, 458, 1253
BmiI GGNNCC 2 cut(s) 120, 1014
BmrFI CCNGG 2 cut(s) 390, 588
BmsI GCATC 1 cut(s) 190
BpmI CTGGAG 3 cut(s) 297, 1317, 1487
Bpu1102I GCTNAGC 2 cut(s) 1107, 1358
Bpu14I TTCGAA 1 cut(s) 1322
BpuEI CTTGAG 1 cut(s) 1187
BsaAI YACGTR 1 cut(s) 1072
BsaBI GATNNNNATC 1 cut(s) 1017
BsaJI CCNNGG 4 cut(s) 81, 985, 1410, 1509
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 593, 1250
Bse1I ACTGG 5 cut(s) 314, 477, 499, 613, 1470
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 2 cut(s) 390, 588
BseDI CCNNGG 4 cut(s) 81, 985, 1410, 1509
BseGI GGATG 6 cut(s) 157, 502, 583, 666, 1265, 1441
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 3 cut(s) 320, 593, 1250
BseMII CTCAG 7 cut(s) 732, 800, 965, 1121, 1209, 1272, 1349
BseNI ACTGG 5 cut(s) 314, 477, 499, 613, 1470
BseSI GKGCMC 1 cut(s) 579
BseXI GCAGC 4 cut(s) 439, 1081, 1326, 1374
BseYI CCCAGC 1 cut(s) 455
BsgI GTGCAG 1 cut(s) 1056
Bsh1285I CGRYCG 1 cut(s) 1196
BshFI GGCC 3 cut(s) 460, 892, 1409
BsiEI CGRYCG 1 cut(s) 1196
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 3 cut(s) 320, 593, 1250
BsmAI GTCTC 3 cut(s) 1060, 1277, 1463
BsmBI CGTCTC 1 cut(s) 1463
BsmFI GGGAC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 1282
BsnI GGCC 3 cut(s) 460, 892, 1409
BsoBI CYCGRG 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 1322
Bsp1286I GDGCHC 1 cut(s) 579
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
Bsp1720I GCTNAGC 2 cut(s) 1107, 1358
Bsp19I CCATGG 1 cut(s) 1410
BspACI CCGC 5 cut(s) 236, 321, 658, 1192, 1482
BspANI GGCC 3 cut(s) 460, 892, 1409
BspCNI CTCAG 7 cut(s) 731, 799, 966, 1120, 1210, 1273, 1350
BspLI GGNNCC 2 cut(s) 120, 1014
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 6 cut(s) 94, 505, 783, 856, 1007, 1020
BspQI GCTCTTC 1 cut(s) 442
BspT104I TTCGAA 1 cut(s) 1322
BsrBI CCGCTC 1 cut(s) 1192
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 5 cut(s) 314, 477, 499, 613, 1470
BssECI CCNNGG 4 cut(s) 81, 985, 1410, 1509
BssMI GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
BssSI CACGAG 1 cut(s) 1212
BssT1I CCWWGG 1 cut(s) 1410
Bst2BI CACGAG 1 cut(s) 1212
Bst2UI CCWGG 2 cut(s) 390, 588
Bst4CI ACNGT 6 cut(s) 175, 224, 382, 527, 1030, 1354
Bst6I CTCTTC 4 cut(s) 159, 442, 815, 826
BstAUI TGTACA 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 1072
BstBI TTCGAA 1 cut(s) 1322
BstDEI CTNAG 9 cut(s) 718, 786, 974, 1058, 1107, 1218, 1239, 1281, 1358
BstDSI CCRYGG 3 cut(s) 985, 1410, 1509
BstF5I GGATG 6 cut(s) 157, 502, 583, 666, 1265, 1441
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 425, 1135
BstKTI GATC 8 cut(s) 89, 404, 500, 521, 703, 778, 864, 1015
BstMAI GTCTC 3 cut(s) 1060, 1277, 1463
BstMBI GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
BstMCI CGRYCG 1 cut(s) 1196
BstMWI GCNNNNNNNGC 5 cut(s) 1103, 1139, 1214, 1415, 1482
BstNI CCWGG 2 cut(s) 390, 588
BstSCI CCNGG 2 cut(s) 388, 586
BstSFI CTRYAG 3 cut(s) 132, 174, 946
BstSLI GKGCMC 1 cut(s) 579
BstV1I GCAGC 4 cut(s) 439, 1081, 1326, 1374
BstX2I RGATCY 2 cut(s) 861, 1012
BstYI RGATCY 2 cut(s) 861, 1012
BsuI GTATCC 1 cut(s) 949
BsuRI GGCC 3 cut(s) 460, 892, 1409
BtgI CCRYGG 3 cut(s) 985, 1410, 1509
BtsCI GGATG 6 cut(s) 157, 502, 583, 666, 1265, 1441
BtsIMutI CAGTG 3 cut(s) 229, 378, 532
BveI ACCTGC 1 cut(s) 241
CfoI GCGC 2 cut(s) 425, 1135
Cfr13I GGNCC 3 cut(s) 437, 458, 1253
CseI GACGC 1 cut(s) 1503
Csp6I GTAC 3 cut(s) 350, 883, 1463
CspCI CAANNNNNGTGG 2 cut(s) 1005, 1040
CviAII CATG 5 cut(s) 281, 567, 751, 760, 1411
CviQI GTAC 3 cut(s) 350, 883, 1463
DdeI CTNAG 9 cut(s) 718, 786, 974, 1058, 1107, 1218, 1239, 1281, 1358
DpnI GATC 8 cut(s) 88, 403, 499, 520, 702, 777, 863, 1014
DpnII GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
DraIII CACNNNGTG 1 cut(s) 527
DrdI GACNNNNNNGTC 1 cut(s) 1451
DseDI GACNNNNNNGTC 1 cut(s) 1451
Eam1104I CTCTTC 4 cut(s) 159, 442, 815, 826
EarI CTCTTC 4 cut(s) 159, 442, 815, 826
EciI GGCGGA 1 cut(s) 673
Eco130I CCWWGG 1 cut(s) 1410
Eco47I GGWCC 2 cut(s) 437, 1253
Eco57I CTGAAG 1 cut(s) 1293
Eco72I CACGTG 1 cut(s) 1072
Eco88I CYCGRG 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 388, 586
EcoT14I CCWWGG 1 cut(s) 1410
ErhI CCWWGG 1 cut(s) 1410
Esp3I CGTCTC 1 cut(s) 1463
FaeI CATG 5 cut(s) 284, 570, 754, 763, 1414
FaqI GGGAC 1 cut(s) 728
FatI CATG 5 cut(s) 280, 566, 750, 759, 1410
FauNDI CATATG 2 cut(s) 561, 727
Fnu4HI GCNGC 5 cut(s) 453, 1095, 1315, 1363, 1483
FokI GGATG 6 cut(s) 164, 489, 570, 673, 1252, 1448
Fsp4HI GCNGC 5 cut(s) 453, 1095, 1315, 1363, 1483
FspBI CTAG 4 cut(s) 51, 72, 738, 1302
GlaI GCGC 2 cut(s) 424, 1134
GluI GCNGC 5 cut(s) 453, 1095, 1315, 1363, 1483
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 3 cut(s) 297, 1317, 1487
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 3 cut(s) 460, 892, 1409
HapII CCGG 1 cut(s) 116
HgaI GACGC 1 cut(s) 1503
HhaI GCGC 2 cut(s) 425, 1135
Hin1II CATG 5 cut(s) 284, 570, 754, 763, 1414
Hin6I GCGC 2 cut(s) 423, 1133
HinP1I GCGC 2 cut(s) 423, 1133
HincII GTYRAC 2 cut(s) 1026, 1444
HindII GTYRAC 2 cut(s) 1026, 1444
HindIII AAGCTT 3 cut(s) 867, 1206, 1416
HinfI GANTC 8 cut(s) 4, 308, 338, 716, 991, 1262, 1319, 1445
HpaII CCGG 1 cut(s) 116
HphI GGTGA 2 cut(s) 333, 1099
Hpy166II GTNNAC 4 cut(s) 220, 1026, 1075, 1444
Hpy188I TCNGA 7 cut(s) 9, 91, 199, 337, 553, 721, 1162
Hpy188III TCNNGA 3 cut(s) 84, 1296, 1372
Hpy8I GTNNAC 4 cut(s) 220, 1026, 1075, 1444
Hpy99I CGWCG 1 cut(s) 1497
HpyAV CCTTC 8 cut(s) 69, 248, 287, 381, 516, 794, 1139, 1317
HpyCH4III ACNGT 6 cut(s) 175, 224, 382, 527, 1030, 1354
HpyCH4IV ACGT 3 cut(s) 855, 1071, 1440
HpyCH4V TGCA 4 cut(s) 269, 698, 750, 1037
HpyF10VI GCNNNNNNNGC 5 cut(s) 1103, 1139, 1214, 1415, 1482
HpyF3I CTNAG 9 cut(s) 718, 786, 974, 1058, 1107, 1218, 1239, 1281, 1358
HpySE526I ACGT 3 cut(s) 855, 1071, 1440
Hsp92II CATG 5 cut(s) 284, 570, 754, 763, 1414
HspAI GCGC 2 cut(s) 423, 1133
Kzo9I GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 2 cut(s) 361, 1298
Lsp1109I GCAGC 4 cut(s) 439, 1081, 1326, 1374
LweI GCATC 1 cut(s) 190
MaeI CTAG 4 cut(s) 51, 72, 738, 1302
MaeII ACGT 3 cut(s) 855, 1071, 1440
MaeIII GTNAC 5 cut(s) 127, 169, 329, 395, 977
MalI GATC 8 cut(s) 88, 403, 499, 520, 702, 777, 863, 1014
MbiI CCGCTC 1 cut(s) 1192
MboI GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
MflI RGATCY 2 cut(s) 861, 1012
MhlI GDGCHC 1 cut(s) 579
MluCI AATT 7 cut(s) 144, 432, 651, 676, 1000, 1122, 1248
MlyI GAGTC 2 cut(s) 710, 1439
MmeI TCCRAC 2 cut(s) 69, 645
MroXI GAANNNNTTC 1 cut(s) 276
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 2 cut(s) 390, 588
Mva1269I GAATGC 1 cut(s) 1282
MvaI CCWGG 2 cut(s) 390, 588
MwoI GCNNNNNNNGC 5 cut(s) 1103, 1139, 1214, 1415, 1482
NcoI CCATGG 1 cut(s) 1410
NdeI CATATG 2 cut(s) 561, 727
NdeII GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
NlaIII CATG 5 cut(s) 284, 570, 754, 763, 1414
NlaIV GGNNCC 2 cut(s) 120, 1014
NspV TTCGAA 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 442
PctI GAATGC 1 cut(s) 1282
PdmI GAANNNNTTC 1 cut(s) 276
PfeI GAWTC 5 cut(s) 308, 338, 991, 1262, 1319
PflMI CCANNNNNTGG 1 cut(s) 1250
PkrI GCNGC 5 cut(s) 454, 1096, 1316, 1364, 1484
PleI GAGTC 2 cut(s) 710, 1439
PmaCI CACGTG 1 cut(s) 1072
PmlI CACGTG 1 cut(s) 1072
PpsI GAGTC 2 cut(s) 710, 1439
Ppu21I YACGTR 1 cut(s) 1072
PsiI TTATAA 1 cut(s) 999
Psp6I CCWGG 2 cut(s) 388, 586
PspCI CACGTG 1 cut(s) 1072
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 2 cut(s) 388, 586
PspN4I GGNNCC 2 cut(s) 120, 1014
PspPI GGNCC 3 cut(s) 437, 458, 1253
PsuI RGATCY 2 cut(s) 861, 1012
RsaI GTAC 3 cut(s) 351, 884, 1464
RsaNI GTAC 3 cut(s) 350, 883, 1463
SapI GCTCTTC 1 cut(s) 442
SatI GCNGC 5 cut(s) 453, 1095, 1315, 1363, 1483
Sau3AI GATC 8 cut(s) 86, 401, 497, 518, 700, 775, 861, 1012
Sau96I GGNCC 3 cut(s) 437, 458, 1253
SchI GAGTC 2 cut(s) 710, 1439
ScrFI CCNGG 2 cut(s) 390, 588
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 1 cut(s) 190
SfcI CTRYAG 3 cut(s) 132, 174, 946
SfuI TTCGAA 1 cut(s) 1322
SinI GGWCC 2 cut(s) 437, 1253
SmlI CTYRAG 1 cut(s) 1166
SmoI CTYRAG 1 cut(s) 1166
Sse9I AATT 7 cut(s) 144, 432, 651, 676, 1000, 1122, 1248
SsiI CCGC 5 cut(s) 236, 321, 658, 1192, 1482
SspI AATATT 1 cut(s) 112
SspMI CTAG 4 cut(s) 51, 72, 738, 1302
StyD4I CCNGG 2 cut(s) 388, 586
StyI CCWWGG 1 cut(s) 1410
TaaI ACNGT 6 cut(s) 175, 224, 382, 527, 1030, 1354
TaiI ACGT 3 cut(s) 858, 1074, 1443
TaqI TCGA 5 cut(s) 688, 1274, 1322, 1371, 1385
TasI AATT 7 cut(s) 144, 432, 651, 676, 1000, 1122, 1248
TatI WGTACW 2 cut(s) 882, 1462
TauI GCSGC 1 cut(s) 1485
TfiI GAWTC 5 cut(s) 308, 338, 991, 1262, 1319
TscAI CASTG 3 cut(s) 229, 385, 532
TseI GCWGC 4 cut(s) 452, 1094, 1314, 1362
TspDTI ATGAA 6 cut(s) 18, 153, 243, 267, 269, 781
TspGWI ACGGA 3 cut(s) 164, 968, 1190
TspRI CASTG 3 cut(s) 229, 385, 532
Van91I CCANNNNNTGG 1 cut(s) 1250
VpaK11BI GGWCC 2 cut(s) 437, 1253
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 4 cut(s) 51, 72, 738, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.