MD11G1168400.v1.1

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003236
Physical Location & Seq
Forward (+)
18024892 .. 18031680
6789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1168400.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGACTCGAATTCTGGTTCAGCGAGGTTCCTCGAGTGGTTCTTCTTCCAACCCAACTCGTCCTTCTTCCTTGTCTGGGTCTTCTTCTGCTTGGGCACAGCCACAGGTTAATAATCCTCAGGTTCTTTCGGTTGTGAAAGATGAGGAAATTGTAGAGGAGGCACAAGAAAATGTTATTTTGGATGAGCTGCCAGAGTATTGTGAGAGTGATGACAATAAGGCTGTGAAAAGTGATGATCTTTTGACAGAAAGCTTTAATGATGACAATGTGGGTCCAAGTGAGGAACGAGCTGATGGGGAGAAAGTTGGTAAAAATGACATTGCAAGCCCAGGGGAATTGATGAAAGGTCTAGACGGGCTGAGGATTTCTGAAAGGGTGACAGTTGAAAATGTGGACTCGAGCAGGGATTCTTTACAAAATGTGATTGGGAGTTCTCAACCACCACCTCCTCCAGTCCCTCCCCCAAAACTTCCCTCGGCAAACTCAAATTCAAGGAGATCTGTCTCAGGAAGTTCAAATGCTGTGCGGATTGGGCCTTCTAGAAGAGCAGTTGCCTGGCCTGTTGTTTCAACTAGGACTTCGCCGAATGGGTCGCGGCCTTCTTCTCCCAGGTCTCATGGTGAAAGCGAGGGGTACAATAGCGCTGATGAGCATAGTCCTTGCTTTGTGTCTTCCTATGATGACACTGAAAGAGAACGACAGTTTGAAATTGATATTAGACGTGCAAAAGGTTTTGAAGTGAAAAAAATGCTGGAGGATGGGAATTGTCTCTTTCGTGCTGTTGCAGATCAAGTTTATGGGGATTCTGAAGCCTATGACTTGATTAGGCAAATGTGCATGGATTACATGGAACGAGAGAGGGATCACTTTTCACAATTTATTACAGAAGGATTTACCTCTTATTGTAAGAGGAAAAGAAGAGACAAGGTATACGGAAACAATGTGGAGATCCAAGCCTTATCTGAAATGTATAATCGGCCCATCCATATTTATTCCTATAGCACAGAACCGATTAATATCTTCCACGGAAGCTATGACACTGACACACCACCCCTACGATTAAGTTATCATCATGGAAATCATTACAACTCTCTTGTTGATCCCCGTCGTCAGACCATCGGTGTAGGACTTGGGTTCAGCCGTCTTCGTGGGTCAAATGTTGACAAGGATCAGGTCAAGGCTGCTATTAAGGCTCAACAAGACCAACAAATTGATAATGCACTTCTAGCTGAGGGGCGATTTTACTCAGATCTGGAGCTTACTGAGAAAGAAATTGAGCGCATGGTGATGGAAGCTTCAAGGCTTGAATATGCAGATGGCACATTTAAGCAACAACTTGGTCGTAGAGAATCTTCCACTTCTAGTGCTGAGCCGTCATCTTCTGGAGCTAGATCATCGGGCAGTGAGACTAAAATGGTAGGTAGGGACAAAGGATTTCAGGACTCAACCCCAAGCAGTAGTATGCAAGTCGTGCTATCGATGGGGTTCAGCTACCTGCAGGTGATCGAGGCATACAACATATTCGGAGATGATGTCGATTCCATGGTTTGTTACTTGTTGGAAACTAGCAGCAGTAGCCGGCGCAAAGGCAAGGCAACCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 map04622
Pfam Domains
Protein Families
OTU

Protein Analysis

535

Amino Acids

59.25

Weight (kDa)

5.11

Isoelectric Point (pI)

64.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 253 - 362 1.4e-18 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1489
Acc36I ACCTGC 2 cut(s) 1489, 1503
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 595
AciI CCGC 2 cut(s) 526, 595
AclWI GGATC 4 cut(s) 870, 943, 1094, 1176
AcsI RAATTY 2 cut(s) 9, 487
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 1 cut(s) 635
AfeI AGCGCT 1 cut(s) 643
AfiI CCNNNNNNNGG 1 cut(s) 75
AgsI TTSAA 8 cut(s) 386, 492, 516, 570, 707, 737, 1299, 1307
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 3 cut(s) 328, 554, 608
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AluBI AGCT 9 cut(s) 187, 252, 290, 1032, 1229, 1258, 1295, 1388, 1491
AluI AGCT 9 cut(s) 187, 252, 290, 1032, 1229, 1258, 1295, 1388, 1491
Alw26I GTCTC 5 cut(s) 508, 618, 773, 915, 1400
AlwI GGATC 4 cut(s) 870, 943, 1094, 1176
Ama87I CYCGRG 2 cut(s) 31, 397
Aor51HI AGCGCT 1 cut(s) 643
AoxI GGCC 4 cut(s) 533, 557, 596, 977
ApeKI GCWGC 3 cut(s) 187, 1181, 1569
ApoI RAATTY 2 cut(s) 9, 487
AseI ATTAAT 1 cut(s) 1014
AspLEI GCGC 3 cut(s) 644, 1281, 1584
AspS9I GGNCC 3 cut(s) 272, 533, 978
AsuHPI GGTGA 4 cut(s) 388, 632, 1297, 1513
AvaI CYCGRG 2 cut(s) 31, 397
AvaII GGWCC 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 97
BarI GAAGNNNNNNTAC 2 cut(s) 1336, 1368
BbsI GAAGAC 3 cut(s) 72, 663, 1136
BbvCI CCTCAGC 2 cut(s) 359, 1230
BbvI GCAGC 3 cut(s) 174, 1168, 1581
BccI CCATC 7 cut(s) 287, 752, 989, 1124, 1282, 1310, 1474
BceAI ACGGC 2 cut(s) 1125, 1357
BciT130I CCWGG 3 cut(s) 330, 556, 610
BcoDI GTCTC 5 cut(s) 508, 618, 773, 915, 1400
BfaI CTAG 7 cut(s) 350, 540, 573, 1226, 1362, 1389, 1566
BfmI CTRYAG 2 cut(s) 997, 1496
BfoI RGCGCY 1 cut(s) 645
BfuAI ACCTGC 2 cut(s) 1489, 1503
BglII AGATCT 2 cut(s) 497, 1249
BisI GCNGC 4 cut(s) 188, 596, 1182, 1570
BlpI GCTNAGC 1 cut(s) 1368
BlsI GCNGC 4 cut(s) 189, 597, 1183, 1571
Bme1390I CCNGG 3 cut(s) 330, 556, 610
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 2 cut(s) 31, 397
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 3 cut(s) 272, 533, 978
BmiI GGNNCC 2 cut(s) 28, 273
BmrFI CCNGG 3 cut(s) 330, 556, 610
BpiI GAAGAC 3 cut(s) 72, 663, 1136
BpmI CTGGAG 4 cut(s) 435, 773, 1274, 1404
Bpu10I CCTNAGC 2 cut(s) 359, 1230
Bpu1102I GCTNAGC 1 cut(s) 1368
Bsa29I ATCGAT 1 cut(s) 1478
BsaBI GATNNNNATC 1 cut(s) 1016
BsaI GGTCTC 1 cut(s) 618
BsaJI CCNNGG 6 cut(s) 328, 329, 474, 608, 1024, 1542
BsaXI ACNNNNNCTCC 2 cut(s) 1518, 1548
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse118I RCCGGY 1 cut(s) 1578
Bse1I ACTGG 1 cut(s) 452
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 1016
BseBI CCWGG 3 cut(s) 330, 556, 610
BseCI ATCGAT 1 cut(s) 1478
BseDI CCNNGG 6 cut(s) 328, 329, 474, 608, 1024, 1542
BseGI GGATG 3 cut(s) 187, 763, 981
BseJI GATNNNNATC 1 cut(s) 1016
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 7 cut(s) 131, 350, 519, 1221, 1254, 1260, 1359
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 2 cut(s) 170, 438
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 3 cut(s) 174, 1168, 1581
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 4 cut(s) 535, 559, 598, 979
BshVI ATCGAT 1 cut(s) 1478
BsiHKCI CYCGRG 2 cut(s) 31, 397
BsiSI CCGG 1 cut(s) 1579
BslFI GGGAC 2 cut(s) 440, 1439
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 5 cut(s) 508, 618, 773, 915, 1400
BsmFI GGGAC 2 cut(s) 440, 1439
BsnI GGCC 4 cut(s) 535, 559, 598, 979
Bso31I GGTCTC 1 cut(s) 618
BsoBI CYCGRG 2 cut(s) 31, 397
Bsp1286I GDGCHC 1 cut(s) 97
Bsp1720I GCTNAGC 1 cut(s) 1368
Bsp19I CCATGG 1 cut(s) 1542
BspACI CCGC 2 cut(s) 526, 595
BspANI GGCC 4 cut(s) 535, 559, 598, 979
BspCNI CTCAG 7 cut(s) 130, 351, 518, 1222, 1255, 1259, 1360
BspDI ATCGAT 1 cut(s) 1478
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 28, 273
BspMAI CTGCAG 1 cut(s) 1500
BspMI ACCTGC 2 cut(s) 1489, 1503
BspPI GGATC 4 cut(s) 870, 943, 1094, 1176
BspQI GCTCTTC 1 cut(s) 538
BspTNI GGTCTC 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 1578
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 1578
BssECI CCNNGG 6 cut(s) 328, 329, 474, 608, 1024, 1542
BssNAI GTATAC 1 cut(s) 931
BssT1I CCWWGG 1 cut(s) 1542
Bst1107I GTATAC 1 cut(s) 931
Bst2UI CCWGG 3 cut(s) 330, 556, 610
Bst4CI ACNGT 2 cut(s) 382, 702
Bst6I CTCTTC 2 cut(s) 538, 913
BstAPI GCANNNNNTGC 1 cut(s) 1471
BstC8I GCNNGC 2 cut(s) 325, 1580
BstDEI CTNAG 7 cut(s) 117, 359, 505, 1230, 1246, 1263, 1368
BstDSI CCRYGG 2 cut(s) 1024, 1542
BstF5I GGATG 3 cut(s) 187, 763, 981
BstFNI CGCG 1 cut(s) 595
BstH2I RGCGCY 1 cut(s) 645
BstHHI GCGC 3 cut(s) 644, 1281, 1584
BstMAI GTCTC 5 cut(s) 508, 618, 773, 915, 1400
BstMWI GCNNNNNNNGC 5 cut(s) 532, 1190, 1226, 1471, 1575
BstNI CCWGG 3 cut(s) 330, 556, 610
BstSCI CCNGG 3 cut(s) 328, 554, 608
BstSFI CTRYAG 2 cut(s) 997, 1496
BstSLI GKGCMC 1 cut(s) 97
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 3 cut(s) 174, 1168, 1581
BstV2I GAAGAC 3 cut(s) 72, 663, 1136
BstX2I RGATCY 3 cut(s) 497, 948, 1249
BstYI RGATCY 3 cut(s) 497, 948, 1249
BstZ17I GTATAC 1 cut(s) 931
Bsu15I ATCGAT 1 cut(s) 1478
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 4 cut(s) 535, 559, 598, 979
BsuTUI ATCGAT 1 cut(s) 1478
BtgI CCRYGG 2 cut(s) 1024, 1542
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 3 cut(s) 187, 763, 981
BtsI GCAGTG 1 cut(s) 1408
BtsIMutI CAGTG 3 cut(s) 684, 1038, 1408
BveI ACCTGC 2 cut(s) 1489, 1503
Cac8I GCNNGC 2 cut(s) 325, 1580
CfoI GCGC 3 cut(s) 644, 1281, 1584
Cfr10I RCCGGY 1 cut(s) 1578
Cfr13I GGNCC 3 cut(s) 272, 533, 978
ClaI ATCGAT 1 cut(s) 1478
Csp6I GTAC 1 cut(s) 634
CviAII CATG 6 cut(s) 617, 838, 847, 1073, 1282, 1543
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 7 cut(s) 117, 359, 505, 1230, 1246, 1263, 1368
Eam1104I CTCTTC 2 cut(s) 538, 913
EarI CTCTTC 2 cut(s) 538, 913
Eco130I CCWWGG 1 cut(s) 1542
Eco31I GGTCTC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 272
Eco47III AGCGCT 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 828
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 2 cut(s) 31, 397
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 328, 554, 608
EcoT14I CCWWGG 1 cut(s) 1542
ErhI CCWWGG 1 cut(s) 1542
FaeI CATG 6 cut(s) 620, 841, 850, 1076, 1285, 1546
FaqI GGGAC 2 cut(s) 440, 1439
FatI CATG 6 cut(s) 616, 837, 846, 1072, 1281, 1542
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 4 cut(s) 188, 596, 1182, 1570
FokI GGATG 3 cut(s) 194, 770, 968
Fsp4HI GCNGC 4 cut(s) 188, 596, 1182, 1570
FspBI CTAG 7 cut(s) 350, 540, 573, 1226, 1362, 1389, 1566
GlaI GCGC 3 cut(s) 643, 1280, 1583
GluI GCNGC 4 cut(s) 188, 596, 1182, 1570
GsuI CTGGAG 4 cut(s) 435, 773, 1274, 1404
HaeII RGCGCY 1 cut(s) 645
HaeIII GGCC 4 cut(s) 535, 559, 598, 979
HapII CCGG 1 cut(s) 1579
HhaI GCGC 3 cut(s) 644, 1281, 1584
Hin1II CATG 6 cut(s) 620, 841, 850, 1076, 1285, 1546
Hin6I GCGC 3 cut(s) 642, 1279, 1582
HinP1I GCGC 3 cut(s) 642, 1279, 1582
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HindIII AAGCTT 2 cut(s) 250, 1293
HinfI GANTC 7 cut(s) 4, 395, 407, 803, 1349, 1442, 1538
HpaII CCGG 1 cut(s) 1579
HphI GGTGA 4 cut(s) 388, 632, 1297, 1513
Hpy166II GTNNAC 3 cut(s) 394, 931, 1162
Hpy188I TCNGA 6 cut(s) 370, 808, 964, 1113, 1249, 1526
Hpy188III TCNNGA 6 cut(s) 350, 507, 540, 1253, 1383, 1439
Hpy8I GTNNAC 3 cut(s) 394, 931, 1162
Hpy99I CGWCG 1 cut(s) 1110
HpyAV CCTTC 4 cut(s) 72, 546, 609, 881
HpyCH4III ACNGT 2 cut(s) 382, 702
HpyCH4IV ACGT 1 cut(s) 721
HpyCH4V TGCA 8 cut(s) 323, 725, 785, 837, 1220, 1313, 1465, 1498
HpyF10VI GCNNNNNNNGC 5 cut(s) 532, 1190, 1226, 1471, 1575
HpyF3I CTNAG 7 cut(s) 117, 359, 505, 1230, 1246, 1263, 1368
HpySE526I ACGT 1 cut(s) 721
Hsp92II CATG 6 cut(s) 620, 841, 850, 1076, 1285, 1546
HspAI GCGC 3 cut(s) 642, 1279, 1582
KroI GCCGGC 1 cut(s) 1578
KroNI GCCGGC 1 cut(s) 1580
LguI GCTCTTC 1 cut(s) 538
LmnI GCTCC 2 cut(s) 1255, 1385
Lsp1109I GCAGC 3 cut(s) 174, 1168, 1581
MaeI CTAG 7 cut(s) 350, 540, 573, 1226, 1362, 1389, 1566
MaeII ACGT 1 cut(s) 721
MaeIII GTNAC 2 cut(s) 376, 1550
MflI RGATCY 3 cut(s) 497, 948, 1249
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 9 cut(s) 9, 147, 335, 487, 708, 763, 875, 1209, 1272
MlyI GAGTC 2 cut(s) 389, 1436
MmeI TCCRAC 2 cut(s) 72, 1539
MroNI GCCGGC 1 cut(s) 1578
MseI TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
MslI CAYNNNNRTG 1 cut(s) 1286
MspI CCGG 1 cut(s) 1579
MspR9I CCNGG 3 cut(s) 330, 556, 610
MvaI CCWGG 3 cut(s) 330, 556, 610
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 5 cut(s) 532, 1190, 1226, 1471, 1575
NaeI GCCGGC 1 cut(s) 1580
NcoI CCATGG 1 cut(s) 1542
NgoMIV GCCGGC 1 cut(s) 1578
NlaIII CATG 6 cut(s) 620, 841, 850, 1076, 1285, 1546
NlaIV GGNNCC 2 cut(s) 28, 273
NmeAIII GCCGAG 1 cut(s) 455
NmuCI GTSAC 1 cut(s) 376
PaeR7I CTCGAG 2 cut(s) 31, 397
PaqCI CACCTGC 1 cut(s) 1489
PasI CCCWGGG 1 cut(s) 329
PciSI GCTCTTC 1 cut(s) 538
PdiI GCCGGC 1 cut(s) 1580
PfeI GAWTC 4 cut(s) 407, 803, 1349, 1538
PkrI GCNGC 4 cut(s) 189, 597, 1183, 1571
PleI GAGTC 2 cut(s) 389, 1436
PpsI GAGTC 2 cut(s) 389, 1436
PshBI ATTAAT 1 cut(s) 1014
Psp6I CCWGG 3 cut(s) 328, 554, 608
PspGI CCWGG 3 cut(s) 328, 554, 608
PspN4I GGNNCC 2 cut(s) 28, 273
PspPI GGNCC 3 cut(s) 272, 533, 978
PspXI VCTCGAGB 2 cut(s) 31, 397
PstI CTGCAG 1 cut(s) 1500
PsuI RGATCY 3 cut(s) 497, 948, 1249
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 1286
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
SatI GCNGC 4 cut(s) 188, 596, 1182, 1570
Sau96I GGNCC 3 cut(s) 272, 533, 978
SbfI CCTGCAGG 1 cut(s) 1500
SchI GAGTC 2 cut(s) 389, 1436
ScrFI CCNGG 3 cut(s) 330, 556, 610
SdaI CCTGCAGG 1 cut(s) 1500
SduI GDGCHC 1 cut(s) 97
SfcI CTRYAG 2 cut(s) 997, 1496
Sfr274I CTCGAG 2 cut(s) 31, 397
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 2 cut(s) 31, 397
SmiMI CAYNNNNRTG 1 cut(s) 1286
SmlI CTYRAG 2 cut(s) 31, 397
SmoI CTYRAG 2 cut(s) 31, 397
Sse8387I CCTGCAGG 1 cut(s) 1500
Sse9I AATT 9 cut(s) 9, 147, 335, 487, 708, 763, 875, 1209, 1272
SsiI CCGC 2 cut(s) 526, 595
SspMI CTAG 7 cut(s) 350, 540, 573, 1226, 1362, 1389, 1566
StyD4I CCNGG 3 cut(s) 328, 554, 608
StyI CCWWGG 1 cut(s) 1542
TaaI ACNGT 2 cut(s) 382, 702
TaiI ACGT 1 cut(s) 724
TaqI TCGA 6 cut(s) 7, 32, 398, 1478, 1506, 1536
TasI AATT 9 cut(s) 9, 147, 335, 487, 708, 763, 875, 1209, 1272
TauI GCSGC 1 cut(s) 598
TfiI GAWTC 4 cut(s) 407, 803, 1349, 1538
Tru1I TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
Tru9I TTAA 6 cut(s) 108, 255, 1014, 1061, 1188, 1326
TscAI CASTG 3 cut(s) 691, 1045, 1408
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 3 cut(s) 187, 1181, 1569
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 1 cut(s) 356
TspGWI ACGGA 2 cut(s) 948, 1041
TspRI CASTG 3 cut(s) 691, 1045, 1408
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 2 cut(s) 9, 487
XbaI TCTAGA 2 cut(s) 349, 539
XhoI CTCGAG 2 cut(s) 31, 397
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 7 cut(s) 350, 540, 573, 1226, 1362, 1389, 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.