MD03G1149500.v1.1

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003236
Physical Location & Seq
Forward (+)
16633928 .. 16641458
7531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1149500.v1.1.491

Sequence Viewer

Length: 1614 bp
ATGACTAGAATTCTGGTTCAGCGAGGTTCTTCAAGTGGTTCTTCTTCCAACCCAACTCGTCCTTCTTCCTTGTCTGGGTCTTCTTCTGCTTGGGCACAGTCACAGGTTAATAATCCTCAGGCTCTTTCGGTTGTGAAAGATGAGGAAATTGTAGAGGAGGCACAAGAAAATGTTTTTGTGGATGAGCTGCCAGAGTATTGTGGGAGTGGTGACAATAAGGCCGTGAAAAATGATGATCTTTTGACAGAAAGCTGTAATGATGACAATGTGAGTTCAAGTGATGAACTAGCTGAGGGGGAAAAAGTTGGTAAAAATGACATTGCCAGTCCTGGGGAATTGATGAAAGGTTTAGACGGGGTGAGGATTTCTGAAAGGGTGACAGTTGAAAATGTGGACTCAAGCAGTGATTCTTTACAAAATGTGATTGCGAGTTCTCAGCCACCACCTCCTCCAGTCCCTCCTCCCCCAAAACTTTCCTCAGCAAACTCGAATTCAAGGAGATCTGTCTCAGGGAGTTCAAATGCTGTCCGGATTGGACCTTCTAGAAGAGCAGCTGCCTGGCCTGTTGTTTCAACTAGGACTTCGCCAAATGGGTCACTGCCTTCTTCTCCCAGGTCCCATGGTGAAAGCGAGGGGTACAATAGTGCTGATGAGCATAGTCCTTGCTTTGTATCTTCCTATGATGACACTGAAAGAGAACGACAGTTTGAAATTGATATTAGGCGTGCAAAAGGTTTTGAAGTGAAAAGAATGCTGGAGGATGGGAATTGTCTCTTTCGTGCTGTTGCAGATCAAGTTTATGGGGATTCTGAAGCCTATGACTTGATCCGGCAAATGTGCATAGATTACATGGAACGAGAGAGGGATCACTTTTCTCAATTTATTACAGAAGGATTTACCTCTTATTGTAAGAGGAAAAGAAGAGACAAGGTCTATGGAAACAATATTGAGATCCAAGCCTTATCTGAAATGTATAATCGGCCCATCCATATTTATTCCTATAGCACAGAACCGATTAATATTTTCCATGGAAGCTATGACACTGACACACCACCCCTACGACTAAGTTATCATCATGGAAATCATTACAACTCTCTTGTTGATCCCCGTCGTCAGACCATCGGTGTAGGACTTGGGTTCAGCCGTCTTCGTGGAATGGGTTCAAATGTTGACAAGGATCAGGTCAAGGCTGCTATTAAGGCTCAACAGGACCAACAAATTGATAATGCACTTCTAGCTGAGGGGCGATTTTACTCGGATCTGGAGCTTACTGAGAAAGAAATTGAGCGTATGGTTATGGAAGCTTCGAGGCTTGAATACGCAGACGGCACATTTAAGCAACAACTTGGACGTAGAGAATCTTCCACTTCTAGTGCTGAGCCGTCATCTTCGGGAGCTAGATCATCAGGCAGTGAGACTAAAGTTGAAGAGAGGGAGCAAGGATTTCAGGATTCGACCCTTAGCAGTAGTATGCAAGTTGTGCTATCGATGGGGTTCAGCTATCTGCAGGTGATTGAGGCATACAGCATATTCGGAGATGATGTTGATTCCATGGTTTGTTACTTGTTGGAAACTAGCAGCAGTAGCCGGCGCAAAGGCAAGGCAACCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 map04622
Pfam Domains
Protein Families
OTU

Protein Analysis

538

Amino Acids

59.29

Weight (kDa)

4.97

Isoelectric Point (pI)

63.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 254 - 363 6.7e-19 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1498
Acc36I ACCTGC 1 cut(s) 1498
AccIII TCCGGA 1 cut(s) 528
AclWI GGATC 6 cut(s) 820, 873, 946, 1097, 1185, 1266
AcsI RAATTY 2 cut(s) 9, 490
AcuI CTGAAG 1 cut(s) 831
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 2 cut(s) 75, 330
AjnI CCWGG 3 cut(s) 328, 557, 611
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
Alw26I GTCTC 4 cut(s) 511, 776, 918, 1409
AlwI GGATC 6 cut(s) 820, 873, 946, 1097, 1185, 1266
Aor13HI TCCGGA 1 cut(s) 528
AoxI GGCC 3 cut(s) 219, 560, 980
ApeKI GCWGC 5 cut(s) 187, 551, 554, 1190, 1578
ApoI RAATTY 2 cut(s) 9, 490
AseI ATTAAT 1 cut(s) 1017
Asp700I GAANNNNTTC 1 cut(s) 1159
AspLEI GCGC 1 cut(s) 1593
AspS9I GGNCC 4 cut(s) 536, 615, 981, 1210
AsuHPI GGTGA 5 cut(s) 221, 370, 388, 635, 1522
AvaII GGWCC 3 cut(s) 536, 615, 1210
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 97
BaeI ACNNNNGTAYC 2 cut(s) 628, 661
BarI GAAGNNNNNNTAC 2 cut(s) 1345, 1377
BbsI GAAGAC 2 cut(s) 72, 1139
BbvCI CCTCAGC 3 cut(s) 291, 478, 1239
BbvI GCAGC 5 cut(s) 174, 541, 563, 1177, 1590
BccI CCATC 4 cut(s) 755, 992, 1127, 1483
BceAI ACGGC 4 cut(s) 206, 1128, 1342, 1366
BciT130I CCWGG 3 cut(s) 330, 559, 613
BcoDI GTCTC 4 cut(s) 511, 776, 918, 1409
BfaI CTAG 8 cut(s) 6, 287, 543, 576, 1235, 1371, 1398, 1575
BfmI CTRYAG 2 cut(s) 1000, 1505
BfuAI ACCTGC 1 cut(s) 1498
BglII AGATCT 1 cut(s) 500
BisI GCNGC 5 cut(s) 188, 552, 555, 1191, 1579
BlpI GCTNAGC 1 cut(s) 1377
BlsI GCNGC 5 cut(s) 189, 553, 556, 1192, 1580
Bme1390I CCNGG 3 cut(s) 330, 559, 613
Bme18I GGWCC 3 cut(s) 536, 615, 1210
BmgT120I GGNCC 4 cut(s) 536, 615, 981, 1210
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 3 cut(s) 330, 559, 613
BpiI GAAGAC 2 cut(s) 72, 1139
BpmI CTGGAG 3 cut(s) 435, 776, 1283
Bpu10I CCTNAGC 4 cut(s) 291, 478, 1239, 1460
Bpu1102I GCTNAGC 1 cut(s) 1377
BpuEI CTTGAG 1 cut(s) 382
Bsa29I ATCGAT 1 cut(s) 1487
BsaJI CCNNGG 5 cut(s) 329, 611, 619, 1027, 1551
BsaWI WCCGGW 1 cut(s) 528
BsaXI ACNNNNNCTCC 2 cut(s) 1527, 1557
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 330
Bse118I RCCGGY 1 cut(s) 1587
Bse1I ACTGG 2 cut(s) 324, 452
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 528
BseBI CCWGG 3 cut(s) 330, 559, 613
BseCI ATCGAT 1 cut(s) 1487
BseDI CCNNGG 5 cut(s) 329, 611, 619, 1027, 1551
BseGI GGATG 3 cut(s) 187, 766, 984
BseLI CCNNNNNNNGG 2 cut(s) 75, 330
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 8 cut(s) 131, 282, 449, 492, 522, 1230, 1263, 1368
BseNI ACTGG 2 cut(s) 324, 452
BseRI GAGGAG 3 cut(s) 170, 438, 450
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 5 cut(s) 174, 541, 563, 1177, 1590
BshFI GGCC 3 cut(s) 221, 562, 982
BshVI ATCGAT 1 cut(s) 1487
BsiSI CCGG 3 cut(s) 529, 829, 1588
BslFI GGGAC 2 cut(s) 440, 601
BslI CCNNNNNNNGG 2 cut(s) 75, 330
BsmAI GTCTC 4 cut(s) 511, 776, 918, 1409
BsmFI GGGAC 2 cut(s) 440, 601
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 3 cut(s) 221, 562, 982
Bsp1286I GDGCHC 1 cut(s) 97
Bsp13I TCCGGA 1 cut(s) 528
Bsp1720I GCTNAGC 1 cut(s) 1377
Bsp19I CCATGG 3 cut(s) 619, 1027, 1551
BspANI GGCC 3 cut(s) 221, 562, 982
BspCNI CTCAG 8 cut(s) 130, 283, 448, 491, 521, 1231, 1264, 1369
BspDI ATCGAT 1 cut(s) 1487
BspEI TCCGGA 1 cut(s) 528
BspLI GGNNCC 1 cut(s) 617
BspMAI CTGCAG 1 cut(s) 1509
BspMI ACCTGC 1 cut(s) 1498
BspPI GGATC 6 cut(s) 820, 873, 946, 1097, 1185, 1266
BspQI GCTCTTC 1 cut(s) 541
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 1587
BsrI ACTGG 2 cut(s) 324, 452
BssAI RCCGGY 1 cut(s) 1587
BssECI CCNNGG 5 cut(s) 329, 611, 619, 1027, 1551
BssT1I CCWWGG 3 cut(s) 619, 1027, 1551
Bst2UI CCWGG 3 cut(s) 330, 559, 613
Bst4CI ACNGT 3 cut(s) 99, 382, 705
Bst6I CTCTTC 3 cut(s) 541, 916, 1422
BstAPI GCANNNNNTGC 1 cut(s) 1480
BstC8I GCNNGC 2 cut(s) 726, 1589
BstDSI CCRYGG 3 cut(s) 619, 1027, 1551
BstF5I GGATG 3 cut(s) 187, 766, 984
BstHHI GCGC 1 cut(s) 1593
BstMAI GTCTC 4 cut(s) 511, 776, 918, 1409
BstMWI GCNNNNNNNGC 4 cut(s) 1199, 1235, 1480, 1584
BstNI CCWGG 3 cut(s) 330, 559, 613
BstSCI CCNGG 3 cut(s) 328, 557, 611
BstSFI CTRYAG 2 cut(s) 1000, 1505
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 5 cut(s) 174, 541, 563, 1177, 1590
BstV2I GAAGAC 2 cut(s) 72, 1139
BstX2I RGATCY 3 cut(s) 500, 951, 1258
BstYI RGATCY 3 cut(s) 500, 951, 1258
Bsu15I ATCGAT 1 cut(s) 1487
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 3 cut(s) 221, 562, 982
BsuTUI ATCGAT 1 cut(s) 1487
BtgI CCRYGG 3 cut(s) 619, 1027, 1551
BtsCI GGATG 3 cut(s) 187, 766, 984
BtsI GCAGTG 3 cut(s) 409, 596, 1417
BtsIMutI CAGTG 5 cut(s) 409, 596, 687, 1041, 1417
BveI ACCTGC 1 cut(s) 1498
Cac8I GCNNGC 2 cut(s) 726, 1589
CfoI GCGC 1 cut(s) 1593
Cfr10I RCCGGY 1 cut(s) 1587
Cfr13I GGNCC 4 cut(s) 536, 615, 981, 1210
ClaI ATCGAT 1 cut(s) 1487
Csp6I GTAC 1 cut(s) 637
CviAII CATG 5 cut(s) 620, 850, 1028, 1076, 1552
CviQI GTAC 1 cut(s) 637
Eam1104I CTCTTC 3 cut(s) 541, 916, 1422
EarI CTCTTC 3 cut(s) 541, 916, 1422
Eco130I CCWWGG 3 cut(s) 619, 1027, 1551
Eco47I GGWCC 3 cut(s) 536, 615, 1210
Eco57I CTGAAG 1 cut(s) 831
Eco81I CCTNAGG 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 615
EcoRI GAATTC 2 cut(s) 9, 490
EcoRII CCWGG 3 cut(s) 328, 557, 611
EcoT14I CCWWGG 3 cut(s) 619, 1027, 1551
ErhI CCWWGG 3 cut(s) 619, 1027, 1551
FaeI CATG 5 cut(s) 623, 853, 1031, 1079, 1555
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 2 cut(s) 440, 601
FatI CATG 5 cut(s) 619, 849, 1027, 1075, 1551
Fnu4HI GCNGC 5 cut(s) 188, 552, 555, 1191, 1579
FokI GGATG 3 cut(s) 194, 773, 971
Fsp4HI GCNGC 5 cut(s) 188, 552, 555, 1191, 1579
FspBI CTAG 8 cut(s) 6, 287, 543, 576, 1235, 1371, 1398, 1575
GlaI GCGC 1 cut(s) 1592
GluI GCNGC 5 cut(s) 188, 552, 555, 1191, 1579
GsuI CTGGAG 3 cut(s) 435, 776, 1283
HaeIII GGCC 3 cut(s) 221, 562, 982
HapII CCGG 3 cut(s) 529, 829, 1588
HhaI GCGC 1 cut(s) 1593
Hin1II CATG 5 cut(s) 623, 853, 1031, 1079, 1555
Hin6I GCGC 1 cut(s) 1591
HinP1I GCGC 1 cut(s) 1591
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 1 cut(s) 1302
HinfI GANTC 6 cut(s) 395, 407, 806, 1358, 1451, 1547
HpaII CCGG 3 cut(s) 529, 829, 1588
HphI GGTGA 5 cut(s) 221, 370, 388, 635, 1522
Hpy166II GTNNAC 2 cut(s) 394, 1171
Hpy188I TCNGA 6 cut(s) 370, 811, 967, 1116, 1258, 1535
Hpy188III TCNNGA 5 cut(s) 529, 543, 1262, 1392, 1448
Hpy8I GTNNAC 2 cut(s) 394, 1171
Hpy99I CGWCG 1 cut(s) 1113
HpyAV CCTTC 4 cut(s) 72, 549, 612, 884
HpyCH4III ACNGT 3 cut(s) 99, 382, 705
HpyCH4IV ACGT 1 cut(s) 1351
HpyCH4V TGCA 6 cut(s) 728, 788, 840, 1229, 1474, 1507
HpyF10VI GCNNNNNNNGC 4 cut(s) 1199, 1235, 1480, 1584
HpySE526I ACGT 1 cut(s) 1351
Hsp92II CATG 5 cut(s) 623, 853, 1031, 1079, 1555
HspAI GCGC 1 cut(s) 1591
Kpn2I TCCGGA 1 cut(s) 528
KroI GCCGGC 1 cut(s) 1587
KroNI GCCGGC 1 cut(s) 1589
LguI GCTCTTC 1 cut(s) 541
LmnI GCTCC 3 cut(s) 1264, 1394, 1435
Lsp1109I GCAGC 5 cut(s) 174, 541, 563, 1177, 1590
MaeI CTAG 8 cut(s) 6, 287, 543, 576, 1235, 1371, 1398, 1575
MaeII ACGT 1 cut(s) 1351
MaeIII GTNAC 5 cut(s) 99, 209, 376, 594, 1559
MflI RGATCY 3 cut(s) 500, 951, 1258
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 9 cut(s) 9, 147, 335, 490, 711, 766, 878, 1218, 1281
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 72, 1548
MroI TCCGGA 1 cut(s) 528
MroNI GCCGGC 1 cut(s) 1587
MroXI GAANNNNTTC 1 cut(s) 1159
MseI TTAA 4 cut(s) 108, 1017, 1197, 1335
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 3 cut(s) 529, 829, 1588
MspR9I CCNGG 3 cut(s) 330, 559, 613
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 3 cut(s) 330, 559, 613
MwoI GCNNNNNNNGC 4 cut(s) 1199, 1235, 1480, 1584
NaeI GCCGGC 1 cut(s) 1589
NcoI CCATGG 3 cut(s) 619, 1027, 1551
NgoMIV GCCGGC 1 cut(s) 1587
NlaIII CATG 5 cut(s) 623, 853, 1031, 1079, 1555
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 4 cut(s) 99, 209, 376, 594
PaqCI CACCTGC 1 cut(s) 1498
PciSI GCTCTTC 1 cut(s) 541
PctI GAATGC 1 cut(s) 756
PdiI GCCGGC 1 cut(s) 1589
PdmI GAANNNNTTC 1 cut(s) 1159
PfeI GAWTC 5 cut(s) 407, 806, 1358, 1451, 1547
PflFI GACNNNGTC 1 cut(s) 929
PkrI GCNGC 5 cut(s) 189, 553, 556, 1192, 1580
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 615
PshBI ATTAAT 1 cut(s) 1017
Psp5II RGGWCCY 1 cut(s) 615
Psp6I CCWGG 3 cut(s) 328, 557, 611
PspGI CCWGG 3 cut(s) 328, 557, 611
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 4 cut(s) 536, 615, 981, 1210
PspPPI RGGWCCY 1 cut(s) 615
PstI CTGCAG 1 cut(s) 1509
PsuI RGATCY 3 cut(s) 500, 951, 1258
PsyI GACNNNGTC 1 cut(s) 929
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
SapI GCTCTTC 1 cut(s) 541
SaqAI TTAA 4 cut(s) 108, 1017, 1197, 1335
SatI GCNGC 5 cut(s) 188, 552, 555, 1191, 1579
Sau96I GGNCC 4 cut(s) 536, 615, 981, 1210
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 3 cut(s) 330, 559, 613
SduI GDGCHC 1 cut(s) 97
SfcI CTRYAG 2 cut(s) 1000, 1505
SinI GGWCC 3 cut(s) 536, 615, 1210
SmlI CTYRAG 1 cut(s) 397
SmoI CTYRAG 1 cut(s) 397
Sse9I AATT 9 cut(s) 9, 147, 335, 490, 711, 766, 878, 1218, 1281
SspI AATATT 2 cut(s) 946, 1021
SspMI CTAG 8 cut(s) 6, 287, 543, 576, 1235, 1371, 1398, 1575
StyD4I CCNGG 3 cut(s) 328, 557, 611
StyI CCWWGG 3 cut(s) 619, 1027, 1551
TaaI ACNGT 3 cut(s) 99, 382, 705
TaiI ACGT 1 cut(s) 1354
TaqI TCGA 4 cut(s) 488, 1307, 1454, 1487
TasI AATT 9 cut(s) 9, 147, 335, 490, 711, 766, 878, 1218, 1281
TfiI GAWTC 5 cut(s) 407, 806, 1358, 1451, 1547
Tru1I TTAA 4 cut(s) 108, 1017, 1197, 1335
Tru9I TTAA 4 cut(s) 108, 1017, 1197, 1335
TscAI CASTG 5 cut(s) 409, 603, 694, 1048, 1417
TseFI GTSAC 4 cut(s) 99, 209, 376, 594
TseI GCWGC 5 cut(s) 187, 551, 554, 1190, 1578
Tsp45I GTSAC 4 cut(s) 99, 209, 376, 594
TspDTI ATGAA 2 cut(s) 297, 356
TspRI CASTG 5 cut(s) 409, 603, 694, 1048, 1417
Tth111I GACNNNGTC 1 cut(s) 929
VpaK11BI GGWCC 3 cut(s) 536, 615, 1210
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 2 cut(s) 9, 490
XbaI TCTAGA 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 1159
XspI CTAG 8 cut(s) 6, 287, 543, 576, 1235, 1371, 1398, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.