AT2G31680

RAB GTPase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012896
Physical Location & Seq
Reverse (-)
13473594 .. 13475085
1492 bp
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UTR
Exon/CDS
Intron
AT2G31680.1

Sequence Viewer

Length: 660 bp
ATGTCGTCCGATGACGAAGGAGGAGAAGAGTATCTATTCAAGATAGTGATAATCGGCGACTCTGCCGTCGGGAAATCGAACCTTCTCTCTCGCTATGCTCGTAACGAGTTCAACGCTCATTCCAAGGCCACGATCGGCGTCGAGTTCCAGACGCAGAATATGGAAATCGAAGGTAAAGAAGTTAAAGCTCAGATTTGGGATACTGCTGGTCAAGAACGGTTTCGTGCCGTCACTTCCGCTTATTATCGCGGCGCCGTCGGAGCTCTCGTCGTTTACGATATTAGTCGTCGATCCACGTTTGAGAGTGTTGGTCGTTGGCTTGATGAGCTCAAGACACATTCGGATACAACGGTTGCGAGGATGCTGGTGGGAAACAAATGCGATCTAGAAAGCATAAGAGCGGTGAGCGTGGAAGAAGGCAAAGCACTAGCTGAAACCGAAGGACTATTTTTCATGGAAACATCAGCTCTCGATTCAACAAATGTTAAAACAGCTTTCGAAATGGTAATCCGTGACATCTATACCAATATAAGCCGGAAACAACTCAACTCCGACACTTATAAAACCGAACTAAGCATGAAAAACAGAGTCAGTCTCGTTAAGGACGATAATAAAAGCTCCACACAAGGCTTTGGTTTCTCTTGCTGTTCTTCCTCGTGA

Protein Analysis

219

Amino Acids

24.36

Weight (kDa)

5.26

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 170 8e-15 ADP-ribosylation factor family
Ras PF00071 14 - 174 9.3e-63 Ras family
Roc PF08477 14 - 128 2.2e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 14 - 110 6.6e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05810 AT1G05810 AT2G31680 AT2G43130
fragaria_vesca FvH4_4g11760
malus_domestica MD04G1033200.v1.1 MD13G1276500.v1.1
prunus_persica Prupe.1G181200_v2.0.a1
pyrus_communis pycom13g28930
rosa_laevigata RLG00000008596
rosa_multiflora Rmu_sc0006766.1_g000004 Rmu_sc0017774.1_g000002
rosa_roxburghii Rroxscaffold_5G00353490
rosa_rugosa Rorug04G0089700
rosa_samantha Rh4AG153400 Rh4BG151500 Rh4CG161900 Rh4DG146800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 561
AasI GACNNNNNNGTC 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 251
AccBSI CCGCTC 1 cut(s) 401
AccII CGCG 1 cut(s) 249
AciI CCGC 3 cut(s) 237, 249, 401
AclWI GGATC 1 cut(s) 285
AcyI GRCGYC 2 cut(s) 138, 252
AgsI TTSAA 3 cut(s) 40, 112, 477
AluBI AGCT 7 cut(s) 188, 263, 328, 431, 467, 494, 618
AluI AGCT 7 cut(s) 188, 263, 328, 431, 467, 494, 618
Alw21I GWGCWC 2 cut(s) 265, 330
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 1 cut(s) 285
AoxI GGCC 1 cut(s) 126
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 415
AsuII TTCGAA 1 cut(s) 498
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 2 cut(s) 265, 330
BauI CACGAG 1 cut(s) 655
Bbv12I GWGCWC 2 cut(s) 265, 330
BceAI ACGGC 3 cut(s) 50, 212, 239
BciVI GTATCC 2 cut(s) 193, 337
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 2 cut(s) 386, 428
BfoI RGCGCY 1 cut(s) 255
BfuI GTATCC 2 cut(s) 193, 337
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 253
BmsI GCATC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 579, 611
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 314
BsaHI GRCGYC 2 cut(s) 138, 252
BsaJI CCNNGG 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 249
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 251
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 265, 330
BsiSI CCGG 1 cut(s) 535
BsmAI GTCTC 1 cut(s) 599
BsnI GGCC 1 cut(s) 128
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 265, 330
Bsp143I GATC 3 cut(s) 132, 290, 382
BspACI CCGC 3 cut(s) 237, 249, 401
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 253
BspPI GGATC 1 cut(s) 285
BspT104I TTCGAA 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 3 cut(s) 132, 290, 382
BssNI GRCGYC 2 cut(s) 138, 252
BssSI CACGAG 1 cut(s) 655
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 655
Bst4CI ACNGT 2 cut(s) 219, 352
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 2 cut(s) 138, 252
BstBI TTCGAA 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 189, 572
BstF5I GGATG 1 cut(s) 366
BstFNI CGCG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 3 cut(s) 135, 293, 385
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 3 cut(s) 132, 290, 382
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 2 cut(s) 260, 325
BstUI CGCG 1 cut(s) 249
BsuI GTATCC 2 cut(s) 193, 337
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 1 cut(s) 366
CfoI GCGC 1 cut(s) 254
CseI GACGC 2 cut(s) 127, 160
CviAII CATG 2 cut(s) 454, 577
DdeI CTNAG 2 cut(s) 189, 572
DinI GGCGCC 1 cut(s) 253
DpnI GATC 3 cut(s) 134, 292, 384
DpnII GATC 3 cut(s) 132, 290, 382
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Ecl136II GAGCTC 2 cut(s) 263, 328
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 2 cut(s) 265, 330
Eco53kI GAGCTC 2 cut(s) 263, 328
EcoICRI GAGCTC 2 cut(s) 263, 328
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 265, 330
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 2 cut(s) 457, 580
FaiI YATR 8 cut(s) 96, 161, 395, 455, 522, 530, 561, 578
FatI CATG 2 cut(s) 453, 576
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 1 cut(s) 373
FriOI GRGCYC 2 cut(s) 265, 330
Fsp4HI GCNGC 1 cut(s) 250
FspBI CTAG 2 cut(s) 386, 428
GlaI GCGC 1 cut(s) 253
GluI GCNGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 535
HgaI GACGC 2 cut(s) 127, 160
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 2 cut(s) 138, 252
Hin1II CATG 2 cut(s) 457, 580
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 3 cut(s) 59, 473, 588
HpaII CCGG 1 cut(s) 535
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 5 cut(s) 10, 192, 260, 343, 553
Hpy188III TCNNGA 8 cut(s) 40, 70, 148, 212, 331, 386, 470, 657
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 5 cut(s) 71, 143, 260, 272, 291
HpyAV CCTTC 5 cut(s) 11, 92, 164, 410, 434
HpyCH4III ACNGT 2 cut(s) 219, 352
HpyCH4IV ACGT 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 325
HpyF3I CTNAG 2 cut(s) 189, 572
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 2 cut(s) 138, 252
Hsp92II CATG 2 cut(s) 457, 580
HspAI GCGC 1 cut(s) 252
KasI GGCGCC 1 cut(s) 251
Kzo9I GATC 3 cut(s) 132, 290, 382
LmnI GCTCC 2 cut(s) 260, 623
LpnPI CCDG 4 cut(s) 161, 192, 350, 548
LweI GCATC 1 cut(s) 351
MaeI CTAG 2 cut(s) 386, 428
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 3 cut(s) 101, 229, 512
MalI GATC 3 cut(s) 134, 292, 384
MbiI CCGCTC 1 cut(s) 401
MboI GATC 3 cut(s) 132, 290, 382
MboII GAAGA 3 cut(s) 38, 425, 642
MhlI GDGCHC 2 cut(s) 265, 330
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 53, 597
MmeI TCCRAC 2 cut(s) 238, 576
MnlI CCTC 2 cut(s) 14, 351
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 3 cut(s) 183, 486, 600
MspI CCGG 1 cut(s) 535
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 260, 325
NarI GGCGCC 1 cut(s) 252
NdeII GATC 3 cut(s) 132, 290, 382
NlaIII CATG 2 cut(s) 457, 580
NlaIV GGNNCC 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 229, 512
NspV TTCGAA 1 cut(s) 498
PcsI WCGNNNNNNNCGW 4 cut(s) 97, 264, 273, 603
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 473
PkrI GCNGC 1 cut(s) 251
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 2 cut(s) 53, 596
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 2 cut(s) 53, 596
PsiI TTATAA 1 cut(s) 561
Psp124BI GAGCTC 2 cut(s) 265, 330
PspN4I GGNNCC 1 cut(s) 253
PvuI CGATCG 1 cut(s) 135
SacI GAGCTC 2 cut(s) 265, 330
SaqAI TTAA 3 cut(s) 183, 486, 600
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 3 cut(s) 132, 290, 382
SchI GAGTC 2 cut(s) 53, 597
SduI GDGCHC 2 cut(s) 265, 330
SfaNI GCATC 1 cut(s) 351
SfoI GGCGCC 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SsiI CCGC 3 cut(s) 237, 249, 401
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 2 cut(s) 386, 428
SstI GAGCTC 2 cut(s) 265, 330
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 219, 352
TaiI ACGT 1 cut(s) 299
TaqI TCGA 6 cut(s) 77, 141, 168, 289, 471, 498
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 3 cut(s) 183, 486, 600
Tru9I TTAA 3 cut(s) 183, 486, 600
TseFI GTSAC 2 cut(s) 229, 512
Tsp45I GTSAC 2 cut(s) 229, 512
TspDTI ATGAA 2 cut(s) 442, 593
TspGWI ACGGA 1 cut(s) 500
XbaI TCTAGA 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 386, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.