Rh4BG151500

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012896
Physical Location & Seq
Forward (+)
27132932 .. 27137574
4643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG151500.1

Sequence Viewer

Length: 663 bp
ATGTCGTCTTCAGACGAGGAGGGATCAGGGGAGGAGTACCTGTTCAAGATCGTCATCATTGGAGACTCAGCGGTGGGCAAATCGAACTTGCTGTCTCGCTATGCGCGGAACGAGTTCAGTGCGCACTCAAAGGCCACAATAGGCGTGGAGTTTCAGACCCAGAGCATGGAAATCGACGGCAAAGAAGTCAAAGCTCAGATTTGGGACACTGCCGGCCAAGAACGCTTCAGGGCTGTAACCTCTGCCTACTACCGCGGCGCCGTCGGAGCTCTCATTGTCTATGATATCACGCGCCGTACCACGTTTGACAGTGTCGGTCGCTGGCTCGATGAGCTCAAGACTCATTCTGATACTACAATGGCGAGGATGCTAGTGGGGAACAAATGTGATTTGGAGAATATTAGGGATGTCAGTGTTGAAGAAGGTAAAAGCCTTGCTGAAGCCGAAGGGTTGTTCTTCATGGAGACATCTGCGCTAGACTCAACCAATGTTAATAAGGCTTTTGAGCTTGTTATTCGAGAAATATATTGCAATGTGAGTCGGAAGGTCTTGAATTCAGATACTTACAAAGCAGAATTATCTGTGAACAGAGTAACCCTGGTTAATAATGGGGCAGATGGATCAAAGAAAGCCCAGAGCTACTTTTCTTGCTGTTCCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

220

Amino Acids

24.36

Weight (kDa)

5.14

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 15 - 172 2.4e-14 ADP-ribosylation factor family
Ras PF00071 16 - 176 7.2e-63 Ras family
Roc PF08477 16 - 130 4.2e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 16 - 112 9.7e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05810 AT1G05810 AT2G31680 AT2G43130
fragaria_vesca FvH4_4g11760
malus_domestica MD04G1033200.v1.1 MD13G1276500.v1.1
prunus_persica Prupe.1G181200_v2.0.a1
pyrus_communis pycom13g28930
rosa_laevigata RLG00000008596
rosa_multiflora Rmu_sc0006766.1_g000004 Rmu_sc0017774.1_g000002
rosa_roxburghii Rroxscaffold_5G00353490
rosa_rugosa Rorug04G0089700
rosa_samantha Rh4AG153400 Rh4BG151500 Rh4CG161900 Rh4DG146800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 257
AccB7I CCANNNNNTGG 1 cut(s) 166
AccII CGCG 3 cut(s) 106, 255, 292
AciI CCGC 4 cut(s) 71, 106, 253, 255
AclWI GGATC 2 cut(s) 31, 628
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 2 cut(s) 211, 459
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 2 cut(s) 38, 298
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 3 cut(s) 46, 419, 553
AjnI CCWGG 1 cut(s) 597
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
AluBI AGCT 5 cut(s) 194, 269, 334, 508, 639
AluI AGCT 5 cut(s) 194, 269, 334, 508, 639
Alw21I GWGCWC 2 cut(s) 271, 336
Alw26I GTCTC 3 cut(s) 57, 99, 458
AlwI GGATC 2 cut(s) 31, 628
AoxI GGCC 2 cut(s) 132, 214
ApoI RAATTY 1 cut(s) 553
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 5 cut(s) 106, 124, 260, 294, 475
BanI GGYRCC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 271, 336
Bbv12I GWGCWC 2 cut(s) 271, 336
BccI CCATC 1 cut(s) 611
BceAI ACGGC 3 cut(s) 193, 245, 279
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 154, 188
BciT130I CCWGG 1 cut(s) 599
BcoDI GTCTC 3 cut(s) 57, 99, 458
BfaI CTAG 2 cut(s) 371, 476
BfoI RGCGCY 1 cut(s) 261
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 599
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 253, 597
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 657
Bse3DI GCAATG 1 cut(s) 538
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 2 cut(s) 253, 597
BseGI GGATG 2 cut(s) 372, 412
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 2 cut(s) 81, 209
BseNI ACTGG 1 cut(s) 657
BseRI GAGGAG 2 cut(s) 32, 47
Bsh1236I CGCG 3 cut(s) 106, 255, 292
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 2 cut(s) 134, 216
BshNI GGYRCC 1 cut(s) 257
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 271, 336
BsiSI CCGG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 3 cut(s) 57, 99, 458
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 134, 216
Bsp1286I GDGCHC 2 cut(s) 271, 336
Bsp143I GATC 3 cut(s) 23, 48, 620
BspACI CCGC 4 cut(s) 71, 106, 253, 255
BspANI GGCC 2 cut(s) 134, 216
BspCNI CTCAG 2 cut(s) 80, 208
BspFNI CGCG 3 cut(s) 106, 255, 292
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 2 cut(s) 31, 628
BspT107I GGYRCC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 1 cut(s) 657
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 2 cut(s) 253, 597
BssMI GATC 3 cut(s) 23, 48, 620
BssNI GRCGYC 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 311
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 214, 323
BstDEI CTNAG 2 cut(s) 67, 195
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 2 cut(s) 372, 412
BstFNI CGCG 3 cut(s) 106, 255, 292
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 5 cut(s) 106, 124, 260, 294, 475
BstKTI GATC 3 cut(s) 26, 51, 623
BstMAI GTCTC 3 cut(s) 57, 99, 458
BstMBI GATC 3 cut(s) 23, 48, 620
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 222, 266, 331
BstNI CCWGG 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 597
BstUI CGCG 3 cut(s) 106, 255, 292
BsuRI GGCC 2 cut(s) 134, 216
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 2 cut(s) 372, 412
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 4 cut(s) 124, 207, 316, 418
Cac8I GCNNGC 2 cut(s) 214, 323
CfoI GCGC 5 cut(s) 106, 124, 260, 294, 475
Cfr10I RCCGGY 1 cut(s) 212
Cfr42I CCGCGG 1 cut(s) 256
Csp6I GTAC 2 cut(s) 37, 297
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviAII CATG 2 cut(s) 166, 460
CviQI GTAC 2 cut(s) 37, 297
DdeI CTNAG 2 cut(s) 67, 195
DinI GGCGCC 1 cut(s) 259
DpnI GATC 3 cut(s) 25, 50, 622
DpnII GATC 3 cut(s) 23, 48, 620
EaeI YGGCCR 1 cut(s) 214
Ecl136II GAGCTC 2 cut(s) 269, 334
Eco24I GRGCYC 2 cut(s) 271, 336
Eco32I GATATC 1 cut(s) 286
Eco53kI GAGCTC 2 cut(s) 269, 334
Eco57I CTGAAG 2 cut(s) 211, 459
EcoICRI GAGCTC 2 cut(s) 269, 334
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 597
EcoRV GATATC 1 cut(s) 286
EcoT38I GRGCYC 2 cut(s) 271, 336
EgeI GGCGCC 1 cut(s) 259
EheI GGCGCC 1 cut(s) 259
FaeI CATG 2 cut(s) 169, 463
FaiI YATR 5 cut(s) 102, 167, 282, 461, 526
FaqI GGGAC 1 cut(s) 218
FatI CATG 2 cut(s) 165, 459
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 2 cut(s) 379, 419
FriOI GRGCYC 2 cut(s) 271, 336
Fsp4HI GCNGC 1 cut(s) 256
FspAI RTGCGCAY 1 cut(s) 123
FspBI CTAG 2 cut(s) 371, 476
FspI TGCGCA 1 cut(s) 123
GlaI GCGC 5 cut(s) 105, 123, 259, 293, 474
GluI GCNGC 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 2 cut(s) 134, 216
HapII CCGG 1 cut(s) 213
HhaI GCGC 5 cut(s) 106, 124, 260, 294, 475
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 169, 463
Hin6I GCGC 5 cut(s) 104, 122, 258, 292, 473
HinP1I GCGC 5 cut(s) 104, 122, 258, 292, 473
HinfI GANTC 4 cut(s) 65, 340, 479, 538
HpaII CCGG 1 cut(s) 213
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 7 cut(s) 13, 156, 198, 266, 349, 543, 559
Hpy188III TCNNGA 4 cut(s) 46, 337, 518, 550
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 2 cut(s) 179, 266
HpyAV CCTTC 3 cut(s) 416, 440, 538
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 1 cut(s) 531
HpyF10VI GCNNNNNNNGC 3 cut(s) 222, 266, 331
HpyF3I CTNAG 2 cut(s) 67, 195
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 169, 463
HspAI GCGC 5 cut(s) 104, 122, 258, 292, 473
KasI GGCGCC 1 cut(s) 257
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 3 cut(s) 23, 48, 620
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 9 cut(s) 12, 53, 173, 214, 226, 307, 584, 611, 647
LweI GCATC 1 cut(s) 357
MaeI CTAG 2 cut(s) 371, 476
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 2 cut(s) 235, 592
MalI GATC 3 cut(s) 25, 50, 622
MboI GATC 3 cut(s) 23, 48, 620
MboII GAAGA 2 cut(s) 431, 448
MhlI GDGCHC 2 cut(s) 271, 336
MluCI AATT 2 cut(s) 553, 575
Mly113I GGCGCC 1 cut(s) 258
MlyI GAGTC 4 cut(s) 59, 334, 473, 547
MmeI TCCRAC 2 cut(s) 244, 521
MnlI CCTC 5 cut(s) 10, 13, 25, 250, 357
MroNI GCCGGC 1 cut(s) 212
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 3 cut(s) 492, 603, 661
MspA1I CMGCKG 2 cut(s) 71, 255
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 1 cut(s) 599
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 3 cut(s) 106, 255, 292
MwoI GCNNNNNNNGC 3 cut(s) 222, 266, 331
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 1 cut(s) 258
NdeII GATC 3 cut(s) 23, 48, 620
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 2 cut(s) 169, 463
NlaIV GGNNCC 1 cut(s) 259
NsbI TGCGCA 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 1 cut(s) 257
PleI GAGTC 4 cut(s) 59, 334, 473, 546
PluTI GGCGCC 1 cut(s) 261
PpsI GAGTC 4 cut(s) 59, 334, 473, 546
Psp124BI GAGCTC 2 cut(s) 271, 336
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 2 cut(s) 38, 298
RsaNI GTAC 2 cut(s) 37, 297
SacI GAGCTC 2 cut(s) 271, 336
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 3 cut(s) 492, 603, 661
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 3 cut(s) 23, 48, 620
SchI GAGTC 4 cut(s) 59, 334, 473, 547
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 2 cut(s) 271, 336
SfaNI GCATC 1 cut(s) 357
SfoI GGCGCC 1 cut(s) 259
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 2 cut(s) 553, 575
SsiI CCGC 4 cut(s) 71, 106, 253, 255
SspDI GGCGCC 1 cut(s) 257
SspI AATATT 1 cut(s) 400
SspMI CTAG 2 cut(s) 371, 476
SstI GAGCTC 2 cut(s) 271, 336
StyD4I CCNGG 1 cut(s) 597
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 305
TaqI TCGA 4 cut(s) 83, 174, 327, 517
TaqII GACCGA 1 cut(s) 305
TasI AATT 2 cut(s) 553, 575
TauI GCSGC 1 cut(s) 258
Tru1I TTAA 3 cut(s) 492, 603, 661
Tru9I TTAA 3 cut(s) 492, 603, 661
TscAI CASTG 4 cut(s) 124, 214, 316, 418
TspDTI ATGAA 1 cut(s) 448
TspRI CASTG 4 cut(s) 124, 214, 316, 418
Tth111I GACNNNGTC 1 cut(s) 311
Van91I CCANNNNNTGG 1 cut(s) 166
XapI RAATTY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 2 cut(s) 371, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.