Rroxscaffold_5G00353490

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012896
Physical Location & Seq
Forward (+)
31322522 .. 31326763
4242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353490.1

Sequence Viewer

Length: 603 bp
ATGGAAATCGACGGCAAAGAAGTCAAGGCTCAGATTTGGGACACTGCCGGCCAAGAACGCTTCAGGGCTGTAACCTCTGCCTACTACCGCGGCGCCGTCGGAGCTCTCGTTGTCTATGATATCACGCGCCGTACCACGTTTGACAGTGTCGGTCGCTGGCTCGATGAGCTCCAGAGTAGTCTTGTTAGTTACTTTGAAATTGTTGACTTGTCTAGAATTTTCCTGATTACATCTTCTCCTGTGTACCTCATTTTCATTTTCTTCTCTCTTTTACCGAAAGCTCATTCTGATACTACAATGGCGAGGATGCTAGTGGGGAACAAATGTGATTTGGAGAATATTAGGGATGTGAGTGTTGAAGAAGGTAAAAGCCTTGCTGAAGCCGAAGGGTTGTTCTTCATGGAGACATCTGCGCTAGACTCAACCAATGTTAATAAGGCTTTCGAGCTTGTTATTCGAGAAATATATAGCAATGTGAGTCGGAAGGTCTTGAATTCAGATACGTACAAAGCAGAGTTATCTGTGAACAGAGTAACCCTGGTTAATAATGGGGCAGATGGATCAAAGAAAGCCCAGGGCTACTTTTCTTGCTGTTCCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

200

Amino Acids

22.35

Weight (kDa)

5.16

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 1 - 62 3.5e-21 Ras family
Roc PF08477 5 - 59 3.6e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 95 - 156 3.6e-16 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05810 AT1G05810 AT2G31680 AT2G43130
fragaria_vesca FvH4_4g11760
malus_domestica MD04G1033200.v1.1 MD13G1276500.v1.1
prunus_persica Prupe.1G181200_v2.0.a1
pyrus_communis pycom13g28930
rosa_laevigata RLG00000008596
rosa_multiflora Rmu_sc0006766.1_g000004 Rmu_sc0017774.1_g000002
rosa_roxburghii Rroxscaffold_5G00353490
rosa_rugosa Rorug04G0089700
rosa_samantha Rh4AG153400 Rh4BG151500 Rh4CG161900 Rh4DG146800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 2 cut(s) 90, 127
AciI CCGC 2 cut(s) 88, 90
AclWI GGATC 1 cut(s) 568
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 2 cut(s) 216, 493
AcuI CTGAAG 2 cut(s) 46, 399
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 3 cut(s) 133, 245, 506
AgsI TTSAA 3 cut(s) 197, 359, 493
AjnI CCWGG 2 cut(s) 537, 573
AluBI AGCT 4 cut(s) 104, 169, 281, 448
AluI AGCT 4 cut(s) 104, 169, 281, 448
Alw21I GWGCWC 2 cut(s) 106, 171
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 568
AoxI GGCC 1 cut(s) 49
ApoI RAATTY 2 cut(s) 216, 493
AspLEI GCGC 3 cut(s) 95, 129, 415
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 2 cut(s) 106, 171
Bbv12I GWGCWC 2 cut(s) 106, 171
BccI CCATC 1 cut(s) 551
BceAI ACGGC 3 cut(s) 28, 80, 114
BciT130I CCWGG 2 cut(s) 539, 575
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 3 cut(s) 213, 311, 416
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 1 cut(s) 91
BlsI GCNGC 1 cut(s) 92
Bme1390I CCNGG 2 cut(s) 539, 575
BmiI GGNNCC 1 cut(s) 94
BmrFI CCNGG 2 cut(s) 539, 575
BmsI GCATC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 88, 537, 573, 574
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 220, 250
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 2 cut(s) 539, 575
BseDI CCNNGG 4 cut(s) 88, 537, 573, 574
BseGI GGATG 2 cut(s) 312, 352
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 597
Bsh1236I CGCG 2 cut(s) 90, 127
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 92
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 106, 171
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 106, 171
Bsp143I GATC 1 cut(s) 560
BspACI CCGC 2 cut(s) 88, 90
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 2 cut(s) 90, 127
BspLI GGNNCC 1 cut(s) 94
BspPI GGATC 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 4 cut(s) 88, 537, 573, 574
BssMI GATC 1 cut(s) 560
BssNI GRCGYC 1 cut(s) 93
Bst2UI CCWGG 2 cut(s) 539, 575
Bst4CI ACNGT 1 cut(s) 146
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 504
BstC8I GCNNGC 2 cut(s) 49, 158
BstDEI CTNAG 1 cut(s) 30
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 2 cut(s) 312, 352
BstFNI CGCG 2 cut(s) 90, 127
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 3 cut(s) 95, 129, 415
BstKTI GATC 1 cut(s) 563
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 1 cut(s) 560
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 57, 101, 166
BstNI CCWGG 2 cut(s) 539, 575
BstSCI CCNGG 2 cut(s) 537, 573
BstSNI TACGTA 1 cut(s) 504
BstUI CGCG 2 cut(s) 90, 127
BsuRI GGCC 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 312, 352
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 42, 151
Cac8I GCNNGC 2 cut(s) 49, 158
CfoI GCGC 3 cut(s) 95, 129, 415
Cfr10I RCCGGY 1 cut(s) 47
Cfr42I CCGCGG 1 cut(s) 91
Csp6I GTAC 3 cut(s) 132, 244, 505
CviAII CATG 1 cut(s) 400
CviQI GTAC 3 cut(s) 132, 244, 505
DdeI CTNAG 1 cut(s) 30
DinI GGCGCC 1 cut(s) 94
DpnI GATC 1 cut(s) 562
DpnII GATC 1 cut(s) 560
EaeI YGGCCR 1 cut(s) 49
Ecl136II GAGCTC 2 cut(s) 104, 169
Eco105I TACGTA 1 cut(s) 504
Eco24I GRGCYC 2 cut(s) 106, 171
Eco32I GATATC 1 cut(s) 121
Eco53kI GAGCTC 2 cut(s) 104, 169
Eco57I CTGAAG 2 cut(s) 46, 399
EcoICRI GAGCTC 2 cut(s) 104, 169
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 2 cut(s) 537, 573
EcoRV GATATC 1 cut(s) 121
EcoT38I GRGCYC 2 cut(s) 106, 171
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
FaeI CATG 1 cut(s) 403
FaiI YATR 4 cut(s) 117, 401, 466, 468
FaqI GGGAC 1 cut(s) 53
FatI CATG 1 cut(s) 399
Fnu4HI GCNGC 1 cut(s) 91
FokI GGATG 2 cut(s) 319, 359
FriOI GRGCYC 2 cut(s) 106, 171
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 3 cut(s) 213, 311, 416
GlaI GCGC 3 cut(s) 94, 128, 414
GluI GCNGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 155
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 48
HhaI GCGC 3 cut(s) 95, 129, 415
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 1 cut(s) 403
Hin6I GCGC 3 cut(s) 93, 127, 413
HinP1I GCGC 3 cut(s) 93, 127, 413
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 2 cut(s) 419, 478
HpaII CCGG 1 cut(s) 48
Hpy166II GTNNAC 3 cut(s) 205, 244, 526
Hpy188I TCNGA 5 cut(s) 33, 101, 289, 483, 499
Hpy188III TCNNGA 5 cut(s) 172, 213, 223, 458, 490
Hpy8I GTNNAC 3 cut(s) 205, 244, 526
Hpy99I CGWCG 2 cut(s) 14, 101
HpyAV CCTTC 3 cut(s) 356, 380, 478
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 2 cut(s) 137, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 101, 166
HpyF3I CTNAG 1 cut(s) 30
HpySE526I ACGT 2 cut(s) 137, 503
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 1 cut(s) 403
HspAI GCGC 3 cut(s) 93, 127, 413
KasI GGCGCC 1 cut(s) 92
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 1 cut(s) 560
LmnI GCTCC 2 cut(s) 101, 174
LweI GCATC 1 cut(s) 297
MaeI CTAG 3 cut(s) 213, 311, 416
MaeII ACGT 2 cut(s) 137, 503
MaeIII GTNAC 3 cut(s) 70, 188, 532
MalI GATC 1 cut(s) 562
MboI GATC 1 cut(s) 560
MboII GAAGA 4 cut(s) 225, 253, 371, 388
MhlI GDGCHC 2 cut(s) 106, 171
MluCI AATT 3 cut(s) 198, 216, 493
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 2 cut(s) 413, 487
MmeI TCCRAC 2 cut(s) 79, 461
MnlI CCTC 3 cut(s) 85, 257, 297
MroNI GCCGGC 1 cut(s) 47
MseI TTAA 3 cut(s) 432, 543, 601
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 539, 575
MvaI CCWGG 2 cut(s) 539, 575
MvnI CGCG 2 cut(s) 90, 127
MwoI GCNNNNNNNGC 3 cut(s) 57, 101, 166
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 1 cut(s) 93
NdeII GATC 1 cut(s) 560
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 1 cut(s) 94
PasI CCCWGGG 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdiI GCCGGC 1 cut(s) 49
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 2 cut(s) 413, 486
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 2 cut(s) 413, 486
Ppu21I YACGTR 1 cut(s) 504
Psp124BI GAGCTC 2 cut(s) 106, 171
Psp6I CCWGG 2 cut(s) 537, 573
PspGI CCWGG 2 cut(s) 537, 573
PspN4I GGNNCC 1 cut(s) 94
PsyI GACNNNGTC 1 cut(s) 146
RsaI GTAC 3 cut(s) 133, 245, 506
RsaNI GTAC 3 cut(s) 132, 244, 505
SacI GAGCTC 2 cut(s) 106, 171
SacII CCGCGG 1 cut(s) 91
SaqAI TTAA 3 cut(s) 432, 543, 601
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 1 cut(s) 560
SchI GAGTC 2 cut(s) 413, 487
ScrFI CCNGG 2 cut(s) 539, 575
SduI GDGCHC 2 cut(s) 106, 171
SfaNI GCATC 1 cut(s) 297
SfoI GGCGCC 1 cut(s) 94
Sfr303I CCGCGG 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 91
SnaBI TACGTA 1 cut(s) 504
Sse9I AATT 3 cut(s) 198, 216, 493
SsiI CCGC 2 cut(s) 88, 90
SspDI GGCGCC 1 cut(s) 92
SspI AATATT 1 cut(s) 340
SspMI CTAG 3 cut(s) 213, 311, 416
SstI GAGCTC 2 cut(s) 106, 171
StyD4I CCNGG 2 cut(s) 537, 573
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 2 cut(s) 140, 506
TaqI TCGA 4 cut(s) 9, 162, 444, 457
TaqII GACCGA 1 cut(s) 140
TasI AATT 3 cut(s) 198, 216, 493
TauI GCSGC 1 cut(s) 93
Tru1I TTAA 3 cut(s) 432, 543, 601
Tru9I TTAA 3 cut(s) 432, 543, 601
TscAI CASTG 2 cut(s) 49, 151
TspDTI ATGAA 2 cut(s) 244, 388
TspRI CASTG 2 cut(s) 49, 151
Tth111I GACNNNGTC 1 cut(s) 146
XapI RAATTY 2 cut(s) 216, 493
XbaI TCTAGA 1 cut(s) 212
XspI CTAG 3 cut(s) 213, 311, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.