MD04G1033200.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012896
Physical Location & Seq
Forward (+)
3752664 .. 3756688
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1033200.v1.1.491

Sequence Viewer

Length: 663 bp
ATGTCGTCTTCAGACGAGGAGGGGTCAGGGGAGGAGTACCTCTTCAAGATCGTCATAATCGGCGACTCGGCGGTGGGGAAATCCAACTTGCTCTCCCGCTACGCGCGGAACGAGTTCAATGCCCACTCCAAGGCGACGATAGGCGTGGAGTTTCAGACCCAGAGCATGGAAATCGACGGCAAAGAGGTCAAAGCTCAGATTTGGGACACCGCCGGACAGGAACGCTTCAGGGCCGTCACCTCTGCTTACTACCGTGGCGCCGTCGGAGCTCTCATTGTCTATGATATCACCCGCAAGACCACTTTCGAGAGTGTTGGCCGCTGGCTCGATGAGCTCAAGACTCATTCCGATACAACTCTGGCAATGATGCTAGTGGGGAACAAATGTGATTTAGAGGATATCAGGGCTGTGAGTGTTGAGGATGGTAGAAGCCTTGCTGAAGCAGAGGGGTTATTCTTCATGGAGACATCTGCCCTGGACTCAACAAATGTTATAAAGGCTTTTGAGCTTGTGATTCAAGAAATATATAACAATGTCAGCCGGAAGGTCTTGAACTCTGATACGTACAAAGCAGAGTTATCTGTGAACAGGGTCAGCCTCGTTAATAATGGAGCAGACGGATCAAAGAAAACCCAGGGCTATTTTTCTTGCTGTTCCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

221

Amino Acids

24.24

Weight (kDa)

4.94

Isoelectric Point (pI)

25.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 15 - 165 4.7e-14 ADP-ribosylation factor family
Ras PF00071 16 - 176 3e-62 Ras family
Roc PF08477 16 - 130 7.1e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 16 - 114 4.9e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05810 AT1G05810 AT2G31680 AT2G43130
fragaria_vesca FvH4_4g11760
malus_domestica MD04G1033200.v1.1 MD13G1276500.v1.1
prunus_persica Prupe.1G181200_v2.0.a1
pyrus_communis pycom13g28930
rosa_laevigata RLG00000008596
rosa_multiflora Rmu_sc0006766.1_g000004 Rmu_sc0017774.1_g000002
rosa_roxburghii Rroxscaffold_5G00353490
rosa_rugosa Rorug04G0089700
rosa_samantha Rh4AG153400 Rh4BG151500 Rh4CG161900 Rh4DG146800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 257
AccB7I CCANNNNNTGG 1 cut(s) 166
AccII CGCG 2 cut(s) 104, 106
AciI CCGC 6 cut(s) 71, 97, 106, 210, 292, 319
AclWI GGATC 1 cut(s) 628
AcoI YGGCCR 1 cut(s) 316
AcuI CTGAAG 2 cut(s) 211, 459
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 2 cut(s) 38, 566
AfiI CCNNNNNNNGG 2 cut(s) 130, 166
AgsI TTSAA 4 cut(s) 46, 118, 518, 553
AjnI CCWGG 2 cut(s) 474, 633
AluBI AGCT 4 cut(s) 194, 269, 334, 508
AluI AGCT 4 cut(s) 194, 269, 334, 508
Alw21I GWGCWC 2 cut(s) 271, 336
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 628
AoxI GGCC 2 cut(s) 231, 316
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 2 cut(s) 106, 260
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 2 cut(s) 229, 280
BanI GGYRCC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 271, 336
Bbv12I GWGCWC 2 cut(s) 271, 336
BccI CCATC 1 cut(s) 416
BceAI ACGGC 3 cut(s) 193, 218, 245
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 154, 188
BciT130I CCWGG 2 cut(s) 476, 635
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 1 cut(s) 371
BfoI RGCGCY 1 cut(s) 261
BisI GCNGC 1 cut(s) 319
BlsI GCNGC 1 cut(s) 320
Bme1390I CCNGG 2 cut(s) 476, 635
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 2 cut(s) 476, 635
BmsI GCATC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 564
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 5 cut(s) 129, 253, 474, 633, 634
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 166
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 476, 635
BseDI CCNNGG 5 cut(s) 129, 253, 474, 633, 634
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 130, 166
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 209
BseRI GAGGAG 2 cut(s) 32, 47
Bsh1236I CGCG 2 cut(s) 104, 106
BshFI GGCC 2 cut(s) 233, 318
BshNI GGYRCC 1 cut(s) 257
BsiHKAI GWGCWC 2 cut(s) 271, 336
BsiSI CCGG 3 cut(s) 213, 541, 657
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 130, 166
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 233, 318
Bsp1286I GDGCHC 2 cut(s) 271, 336
Bsp143I GATC 2 cut(s) 48, 620
BspACI CCGC 6 cut(s) 71, 97, 106, 210, 292, 319
BspANI GGCC 2 cut(s) 233, 318
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 2 cut(s) 104, 106
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 1 cut(s) 628
BspT107I GGYRCC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 369
BssECI CCNNGG 5 cut(s) 129, 253, 474, 633, 634
BssMI GATC 2 cut(s) 48, 620
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 476, 635
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 323
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 2 cut(s) 104, 106
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 2 cut(s) 106, 260
BstKTI GATC 2 cut(s) 51, 623
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 2 cut(s) 48, 620
BstMWI GCNNNNNNNGC 2 cut(s) 266, 331
BstNI CCWGG 2 cut(s) 476, 635
BstSCI CCNGG 2 cut(s) 474, 633
BstSNI TACGTA 1 cut(s) 564
BstUI CGCG 2 cut(s) 104, 106
BsuRI GGCC 2 cut(s) 233, 318
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 323
CfoI GCGC 2 cut(s) 106, 260
Cfr13I GGNCC 1 cut(s) 231
Csp6I GTAC 2 cut(s) 37, 565
CviAII CATG 2 cut(s) 166, 460
CviQI GTAC 2 cut(s) 37, 565
DdeI CTNAG 1 cut(s) 195
DinI GGCGCC 1 cut(s) 259
DpnI GATC 2 cut(s) 50, 622
DpnII GATC 2 cut(s) 48, 620
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Ecl136II GAGCTC 2 cut(s) 269, 334
Eco105I TACGTA 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 271, 336
Eco32I GATATC 2 cut(s) 286, 400
Eco53kI GAGCTC 2 cut(s) 269, 334
Eco57I CTGAAG 2 cut(s) 211, 459
EcoICRI GAGCTC 2 cut(s) 269, 334
EcoRII CCWGG 2 cut(s) 474, 633
EcoRV GATATC 2 cut(s) 286, 400
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 271, 336
EgeI GGCGCC 1 cut(s) 259
EheI GGCGCC 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 169, 463
FaiI YATR 7 cut(s) 56, 167, 282, 461, 494, 526, 528
FaqI GGGAC 1 cut(s) 218
FatI CATG 2 cut(s) 165, 459
FauI CCCGC 2 cut(s) 104, 299
Fnu4HI GCNGC 1 cut(s) 319
FokI GGATG 1 cut(s) 434
FriOI GRGCYC 2 cut(s) 271, 336
Fsp4HI GCNGC 1 cut(s) 319
FspBI CTAG 1 cut(s) 371
GlaI GCGC 2 cut(s) 105, 259
GluI GCNGC 1 cut(s) 319
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 2 cut(s) 233, 318
HapII CCGG 3 cut(s) 213, 541, 657
HhaI GCGC 2 cut(s) 106, 260
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 169, 463
Hin6I GCGC 2 cut(s) 104, 258
HinP1I GCGC 2 cut(s) 104, 258
HinfI GANTC 4 cut(s) 65, 340, 479, 514
HpaII CCGG 3 cut(s) 213, 541, 657
HphI GGTGA 2 cut(s) 229, 280
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 6 cut(s) 13, 156, 198, 266, 349, 559
Hpy188III TCNNGA 5 cut(s) 46, 307, 337, 518, 550
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 3 cut(s) 139, 179, 266
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4IV ACGT 1 cut(s) 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 331
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 563
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 169, 463
HspAI GCGC 2 cut(s) 104, 258
KasI GGCGCC 1 cut(s) 257
Kzo9I GATC 2 cut(s) 48, 620
LmnI GCTCC 2 cut(s) 266, 611
LweI GCATC 1 cut(s) 357
MaeI CTAG 1 cut(s) 371
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 1 cut(s) 235
MalI GATC 2 cut(s) 50, 622
MboI GATC 2 cut(s) 48, 620
MboII GAAGA 2 cut(s) 34, 448
MhlI GDGCHC 2 cut(s) 271, 336
Mly113I GGCGCC 1 cut(s) 258
MlyI GAGTC 3 cut(s) 59, 334, 473
MmeI TCCRAC 2 cut(s) 108, 244
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 213, 541, 657
MspR9I CCNGG 2 cut(s) 476, 635
MvaI CCWGG 2 cut(s) 476, 635
MvnI CGCG 2 cut(s) 104, 106
MwoI GCNNNNNNNGC 2 cut(s) 266, 331
NarI GGCGCC 1 cut(s) 258
NdeII GATC 2 cut(s) 48, 620
NlaIII CATG 2 cut(s) 169, 463
NlaIV GGNNCC 1 cut(s) 259
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 235
PasI CCCWGGG 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 514
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 1 cut(s) 320
PleI GAGTC 3 cut(s) 59, 334, 473
PluTI GGCGCC 1 cut(s) 261
PpsI GAGTC 3 cut(s) 59, 334, 473
Ppu21I YACGTR 1 cut(s) 564
PsiI TTATAA 1 cut(s) 494
Psp124BI GAGCTC 2 cut(s) 271, 336
Psp6I CCWGG 2 cut(s) 474, 633
PspGI CCWGG 2 cut(s) 474, 633
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 231
RsaI GTAC 2 cut(s) 38, 566
RsaNI GTAC 2 cut(s) 37, 565
SacI GAGCTC 2 cut(s) 271, 336
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 1 cut(s) 319
Sau3AI GATC 2 cut(s) 48, 620
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 3 cut(s) 59, 334, 473
ScrFI CCNGG 2 cut(s) 476, 635
SduI GDGCHC 2 cut(s) 271, 336
SetI ASST 9 cut(s) 42, 189, 196, 242, 271, 336, 510, 549, 566
SfaNI GCATC 1 cut(s) 357
SfoI GGCGCC 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SnaBI TACGTA 1 cut(s) 564
SsiI CCGC 6 cut(s) 71, 97, 106, 210, 292, 319
SspDI GGCGCC 1 cut(s) 257
SspMI CTAG 1 cut(s) 371
SstI GAGCTC 2 cut(s) 271, 336
StyD4I CCNGG 2 cut(s) 474, 633
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 254
TaiI ACGT 1 cut(s) 566
TaqI TCGA 3 cut(s) 174, 306, 327
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TseFI GTSAC 1 cut(s) 235
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 1 cut(s) 633
Van91I CCANNNNNTGG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.