FvH4_4g11760

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012896
Physical Location & Seq
Forward (+)
15491378 .. 15495164
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11760.t1

Sequence Viewer

Length: 663 bp
ATGTCGTCTTCAGACGAGGAGGGATCAGGGGAGGAGTACCTCTTCAAGATCGTCATCATCGGCGACTCTGCCGTCGGGAAATCCAACTTGCTCTCTCGCTACGCCCGGAACGAGTTCAGCGCGCACTCCAAGGCCACTATAGGAGTGGAGTTTCAGACCCAGAGCATGGAAATCGACGGCAAAGAAGTCAAGGCTCAGATTTGGGACACTGCCGGCCAAGAACGCTTCAGGGCCGTCACCTCTGCTTACTACCGCGGCGCCGTCGGAGCTCTCATTGTGTATGATATCACTCGCCGGACAACGTTTGAGAGTGTCGGTCGTTGGCTTGATGAGCTCAAGACTCATTCAGATACTACAATGGCGATGATGCTAGTGGGGAACAAATGTGATTTGGAGAATATTAGGAATGTGAGTGTTGAAGAAGGTAAAAGCCTTGCTGAAGCTGAAGGGATGTTCTTCATGGAGACTTCTGCATTAGACTCGACCAATGTTGATAAGGCTTTTGAGCTTGTTATTCGAGAAATATATAGCACCGTGAGCCGAAAGGTCTTGAATTCAGACACTTACAAAGCAGAGTTATCTGTGAACAGAGTAAGCCTGGTTAGTAATGGGGCAGAAGGATCGAAGAAATCCCAGGGCTACTTTTCTTGCTGTTCCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

221

Amino Acids

24.37

Weight (kDa)

5.16

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 15 - 173 4.7e-15 ADP-ribosylation factor family
Ras PF00071 16 - 176 8.6e-64 Ras family
Roc PF08477 16 - 130 5.8e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 16 - 115 5.8e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05810 AT1G05810 AT2G31680 AT2G43130
fragaria_vesca FvH4_4g11760
malus_domestica MD04G1033200.v1.1 MD13G1276500.v1.1
prunus_persica Prupe.1G181200_v2.0.a1
pyrus_communis pycom13g28930
rosa_laevigata RLG00000008596
rosa_multiflora Rmu_sc0006766.1_g000004 Rmu_sc0017774.1_g000002
rosa_roxburghii Rroxscaffold_5G00353490
rosa_rugosa Rorug04G0089700
rosa_samantha Rh4AG153400 Rh4BG151500 Rh4CG161900 Rh4DG146800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 257
AccB7I CCANNNNNTGG 1 cut(s) 166
AccII CGCG 2 cut(s) 122, 255
AciI CCGC 2 cut(s) 253, 255
AclI AACGTT 1 cut(s) 302
AclWI GGATC 2 cut(s) 31, 628
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 3 cut(s) 211, 459, 465
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 3 cut(s) 46, 419, 553
AjnI CCWGG 2 cut(s) 597, 633
AluBI AGCT 4 cut(s) 269, 334, 443, 508
AluI AGCT 4 cut(s) 269, 334, 443, 508
Alw21I GWGCWC 2 cut(s) 271, 336
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 2 cut(s) 31, 628
AoxI GGCC 3 cut(s) 132, 214, 231
ApoI RAATTY 1 cut(s) 553
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 3 cut(s) 122, 124, 260
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 229
BanI GGYRCC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 271, 336
Bbv12I GWGCWC 2 cut(s) 271, 336
BceAI ACGGC 4 cut(s) 56, 193, 218, 245
BcgI CGANNNNNNTGC 6 cut(s) 154, 188, 462, 496, 603, 637
BciT130I CCWGG 2 cut(s) 599, 635
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 1 cut(s) 138
BfoI RGCGCY 1 cut(s) 261
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 3 cut(s) 106, 599, 635
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 3 cut(s) 106, 599, 635
BmsI GCATC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 4 cut(s) 129, 253, 633, 634
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse118I RCCGGY 1 cut(s) 212
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 2 cut(s) 599, 635
BseDI CCNNGG 4 cut(s) 129, 253, 633, 634
BseGI GGATG 1 cut(s) 456
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 209
BsePI GCGCGC 1 cut(s) 120
BseRI GAGGAG 2 cut(s) 32, 47
Bsh1236I CGCG 2 cut(s) 122, 255
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 3 cut(s) 134, 216, 233
BshNI GGYRCC 1 cut(s) 257
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 271, 336
BsiSI CCGG 3 cut(s) 106, 213, 295
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 3 cut(s) 134, 216, 233
Bsp1286I GDGCHC 2 cut(s) 271, 336
Bsp143I GATC 3 cut(s) 23, 48, 620
BspACI CCGC 2 cut(s) 253, 255
BspANI GGCC 3 cut(s) 134, 216, 233
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 2 cut(s) 122, 255
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 2 cut(s) 31, 628
BspT107I GGYRCC 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 4 cut(s) 129, 253, 633, 634
BssHII GCGCGC 1 cut(s) 120
BssMI GATC 3 cut(s) 23, 48, 620
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 599, 635
Bst4CI ACNGT 1 cut(s) 535
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 122, 214
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 2 cut(s) 122, 255
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 3 cut(s) 122, 124, 260
BstKTI GATC 3 cut(s) 26, 51, 623
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 3 cut(s) 23, 48, 620
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 4 cut(s) 222, 266, 331, 537
BstNI CCWGG 2 cut(s) 599, 635
BstSCI CCNGG 3 cut(s) 104, 597, 633
BstSFI CTRYAG 1 cut(s) 138
BstUI CGCG 2 cut(s) 122, 255
BsuRI GGCC 3 cut(s) 134, 216, 233
BtgI CCRYGG 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 1 cut(s) 456
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 122, 214
CfoI GCGC 3 cut(s) 122, 124, 260
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 231
Cfr42I CCGCGG 1 cut(s) 256
Csp6I GTAC 1 cut(s) 37
CviAII CATG 2 cut(s) 166, 460
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 1 cut(s) 195
DinI GGCGCC 1 cut(s) 259
DpnI GATC 3 cut(s) 25, 50, 622
DpnII GATC 3 cut(s) 23, 48, 620
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Ecl136II GAGCTC 2 cut(s) 269, 334
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 271, 336
Eco32I GATATC 1 cut(s) 286
Eco53kI GAGCTC 2 cut(s) 269, 334
Eco57I CTGAAG 3 cut(s) 211, 459, 465
EcoICRI GAGCTC 2 cut(s) 269, 334
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 2 cut(s) 597, 633
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 271, 336
EgeI GGCGCC 1 cut(s) 259
EheI GGCGCC 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 169, 463
FaiI YATR 6 cut(s) 140, 167, 282, 461, 526, 528
FaqI GGGAC 1 cut(s) 218
FatI CATG 2 cut(s) 165, 459
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 1 cut(s) 463
FriOI GRGCYC 2 cut(s) 271, 336
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 1 cut(s) 371
GlaI GCGC 3 cut(s) 121, 123, 259
GluI GCNGC 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 3 cut(s) 134, 216, 233
HapII CCGG 3 cut(s) 106, 213, 295
HhaI GCGC 3 cut(s) 122, 124, 260
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 169, 463
Hin6I GCGC 3 cut(s) 120, 122, 258
HinP1I GCGC 3 cut(s) 120, 122, 258
HinfI GANTC 3 cut(s) 65, 340, 479
HpaII CCGG 3 cut(s) 106, 213, 295
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 6 cut(s) 13, 156, 198, 266, 349, 559
Hpy188III TCNNGA 5 cut(s) 46, 76, 337, 518, 550
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 3 cut(s) 77, 179, 266
HpyAV CCTTC 3 cut(s) 416, 440, 611
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 266, 331, 537
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 169, 463
HspAI GCGC 3 cut(s) 120, 122, 258
KasI GGCGCC 1 cut(s) 257
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 3 cut(s) 23, 48, 620
LmnI GCTCC 1 cut(s) 266
LweI GCATC 1 cut(s) 357
MaeI CTAG 1 cut(s) 371
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 235
MalI GATC 3 cut(s) 25, 50, 622
MboI GATC 3 cut(s) 23, 48, 620
MboII GAAGA 4 cut(s) 34, 431, 448, 637
MhlI GDGCHC 2 cut(s) 271, 336
MluCI AATT 1 cut(s) 553
Mly113I GGCGCC 1 cut(s) 258
MlyI GAGTC 3 cut(s) 59, 334, 473
MmeI TCCRAC 2 cut(s) 108, 244
MnlI CCTC 5 cut(s) 10, 13, 25, 50, 250
MroNI GCCGGC 1 cut(s) 212
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 1 cut(s) 661
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 3 cut(s) 106, 213, 295
MspR9I CCNGG 3 cut(s) 106, 599, 635
MvaI CCWGG 2 cut(s) 599, 635
MvnI CGCG 2 cut(s) 122, 255
MwoI GCNNNNNNNGC 4 cut(s) 222, 266, 331, 537
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 1 cut(s) 258
NciI CCSGG 1 cut(s) 106
NdeII GATC 3 cut(s) 23, 48, 620
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 2 cut(s) 169, 463
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 235
PasI CCCWGGG 1 cut(s) 634
PauI GCGCGC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdiI GCCGGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 1 cut(s) 257
PleI GAGTC 3 cut(s) 59, 334, 473
PluTI GGCGCC 1 cut(s) 261
PpsI GAGTC 3 cut(s) 59, 334, 473
Psp124BI GAGCTC 2 cut(s) 271, 336
Psp1406I AACGTT 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 597, 633
PspGI CCWGG 2 cut(s) 597, 633
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 231
PteI GCGCGC 1 cut(s) 120
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SacI GAGCTC 2 cut(s) 271, 336
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 1 cut(s) 661
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 3 cut(s) 23, 48, 620
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 3 cut(s) 59, 334, 473
ScrFI CCNGG 3 cut(s) 106, 599, 635
SduI GDGCHC 2 cut(s) 271, 336
SetI ASST 9 cut(s) 42, 242, 271, 305, 336, 427, 445, 510, 549
SfaNI GCATC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 138
SfoI GGCGCC 1 cut(s) 259
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 1 cut(s) 553
SsiI CCGC 2 cut(s) 253, 255
SspDI GGCGCC 1 cut(s) 257
SspI AATATT 1 cut(s) 400
SspMI CTAG 1 cut(s) 371
SstI GAGCTC 2 cut(s) 271, 336
StyD4I CCNGG 3 cut(s) 104, 597, 633
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 535
TaiI ACGT 1 cut(s) 305
TaqI TCGA 4 cut(s) 174, 482, 517, 623
TaqII GACCGA 1 cut(s) 305
TasI AATT 1 cut(s) 553
TauI GCSGC 1 cut(s) 258
Tru1I TTAA 1 cut(s) 661
Tru9I TTAA 1 cut(s) 661
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 235
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 448
TspRI CASTG 1 cut(s) 214
Van91I CCANNNNNTGG 1 cut(s) 166
XapI RAATTY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.