AT2G32990

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004610
Physical Location & Seq
Forward (+)
14003128 .. 14006065
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32990.1

Sequence Viewer

Length: 1578 bp
ATGACTGTGATGAATCACCGACACAAGCAATGTCATTTTCTCCTCCTCCTTCTGATCACTGTCTTCTCTGCCGCGTTGGATGGTGTTTCTTCCGAGACAGACGTCGGAGGGTTTGACTACGGTGAAGCATTGTCTAAAAGCTTACTCTACTTTGAAGCTCAGAGATCAGGAAGACTTCCGTACAACCAGCGCGTGACTTGGCGTGATCACTCTGGTCTCACCGATGGTCTTGAACAAGGGGTTGATTTAGTGGGAGGATATCACGACGCCGGAGACCATGTTAAATTTGGACTTCCGATGGCTTTTACGGTGACAATGTTGTCATGGAGTGTAATAGAGTACGGTGACTCACTCGCTTCCACCGGAGAGTTATCTCACGCGCTTGAAGCCATCAAGTGGGGAACAGACTACTTCATCAAGGCACACACAAGCCCAAATGTCTTATGGGCCGAAGTTGGCGACGGTGACACTGACCACTACTGTTGGCAAAGACCGGAGGACATGACGACTTCACGGAGAGCTTTCAAGATCGATGAGAATAATCCGGGATCAGATATCGCTGGAGAAACTGCGGCGGCTATGGCGGCAGCTTCGATCGTTTTCCGTTCAACAAATCCACATTACTCTCACCTTCTCTTGCACCATGCCCAACAATTGTTTGAGTTTGGAGACAAGTACAGAGGAAAATACGATGAGAGTCTAAAAGTTGTGAAAAGTTATTATGCCTCGGTGAGTGGGTACATGGACGAGCTTTTATGGGGTGCCACTTGGCTTTATCGAGCCACCGACAATGAGCACTATATGAGTTATGTGGTTGACATGGCTCACCAACTAGGTGGCCTTTCTTGGGCCATGTCCGAGTTTAGCTGGGACGTCAAGTTCGCCGGTGTTCAACTCCTAGCTTCCATGTTGCTAAAAGAAGAAAAGCACAAGCAACATTCTAAAGTCCTACAACAATACAAATCAAAGGCGGATCATTACCTATGCTCAATTCTCAACAAGAACATCAACGGCACAAACGTCCAGCGAACTCCTGCCGGTTTACTCTACGTCCGACAATGGAACAATATGCAATACGTATCCACAGCATCTTTCCTCCTAACCGTATATTCTGATCATCTCCGAAAATCAAACACCGATCTCGAGTGTCACGAAGGAACCGTAACTCCTGACGAGATGCTTGGCTTTGCGAAATCTCAAATCGATTACATTTTAGGATCAAATCCAATGGAAACAAGTTATCTAGTCGGGTACGGTCCAAAATACCCGATTAGGGTTCATCATCGAGGCGCGTCTATCGCTTCTTTTAAAGAGCACAAAGGTTTTATAGGATGTACTCAAGGATATGATAATTGGTATGGACGGTCCGAGCCAAATCCGAGTGTTTTAGTCGGGGCTTTGGTTGGAGGACCGGATCACCAGGATGATTTTGATGACCGGAGGGGTAATTACGTTCAAACGGAGGCTTGTACGTACAATACTGCTCCTTTGGTCGGTGTGTTTGCGAGACTTATAGAGCTTGAGGAGCAAAAACTTGAAGAAGAAGATGTGAGCTTGGTTGCAACTTATAAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

525

Amino Acids

59.09

Weight (kDa)

5.65

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 40 - 500 1.7e-166 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1569
AasI GACNNNNNNGTC 1 cut(s) 319
AatII GACGTC 2 cut(s) 105, 876
AccB1I GGYRCC 1 cut(s) 761
AccB7I CCANNNNNTGG 1 cut(s) 396
AccII CGCG 4 cut(s) 74, 192, 380, 1292
AciI CCGC 5 cut(s) 72, 572, 575, 584, 971
AclWI GGATC 4 cut(s) 556, 981, 1225, 1422
AcsI RAATTY 1 cut(s) 284
AcyI GRCGYC 3 cut(s) 102, 267, 873
AfaI GTAC 8 cut(s) 182, 341, 677, 740, 1253, 1336, 1471, 1475
AfiI CCNNNNNNNGG 4 cut(s) 396, 847, 1273, 1493
AgsI TTSAA 8 cut(s) 155, 233, 386, 526, 609, 893, 1457, 1538
AjnI CCWGG 1 cut(s) 1419
AloI GAACNNNNNNTCC 4 cut(s) 863, 895, 1150, 1182
AluBI AGCT 9 cut(s) 141, 158, 521, 590, 751, 867, 902, 1519, 1554
AluI AGCT 9 cut(s) 141, 158, 521, 590, 751, 867, 902, 1519, 1554
Alw21I GWGCWC 2 cut(s) 798, 1317
Alw26I GTCTC 5 cut(s) 89, 221, 267, 663, 1501
AlwI GGATC 4 cut(s) 556, 981, 1225, 1422
Ama87I CYCGRG 1 cut(s) 1142
AoxI GGCC 3 cut(s) 447, 838, 849
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 1 cut(s) 284
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AspLEI GCGC 3 cut(s) 192, 382, 1292
AspS9I GGNCC 5 cut(s) 447, 849, 1256, 1365, 1409
AsuC2I CCSGG 1 cut(s) 546
AvaI CYCGRG 1 cut(s) 1142
AvaII GGWCC 3 cut(s) 1256, 1365, 1409
BanI GGYRCC 1 cut(s) 761
BbsI GAAGAC 2 cut(s) 55, 178
Bbv12I GWGCWC 2 cut(s) 798, 1317
BbvI GCAGC 1 cut(s) 599
BccI CCATC 4 cut(s) 74, 218, 292, 398
BceAI ACGGC 1 cut(s) 1027
BciT130I CCWGG 1 cut(s) 1421
BciVI GTATCC 1 cut(s) 1090
BclI TGATCA 3 cut(s) 54, 205, 1114
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 5 cut(s) 89, 221, 267, 663, 1501
BfaI CTAG 3 cut(s) 833, 899, 1244
BfuI GTATCC 1 cut(s) 1090
BisI GCNGC 5 cut(s) 72, 573, 576, 585, 588
BlsI GCNGC 5 cut(s) 73, 574, 577, 586, 589
Bme1390I CCNGG 2 cut(s) 546, 1421
Bme18I GGWCC 3 cut(s) 1256, 1365, 1409
BmeT110I CYCGRG 1 cut(s) 1142
BmgT120I GGNCC 5 cut(s) 447, 849, 1256, 1365, 1409
BmiI GGNNCC 2 cut(s) 763, 1159
BmrFI CCNGG 2 cut(s) 546, 1421
BmsI GCATC 2 cut(s) 1097, 1167
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 2 cut(s) 55, 178
BpmI CTGGAG 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 1323, 1541
BpuMI CCSGG 1 cut(s) 546
Bsa29I ATCGAT 2 cut(s) 531, 1203
BsaAI YACGTR 2 cut(s) 1078, 1473
BsaHI GRCGYC 3 cut(s) 102, 267, 873
BsaI GGTCTC 2 cut(s) 221, 267
BsaJI CCNNGG 1 cut(s) 726
BsaWI WCCGGW 4 cut(s) 362, 493, 1411, 1437
BsaXI ACNNNNNCTCC 4 cut(s) 264, 294, 1150, 1180
Bsc4I CCNNNNNNNGG 4 cut(s) 396, 847, 1273, 1493
Bse118I RCCGGY 2 cut(s) 884, 1037
Bse3DI GCAATG 1 cut(s) 35
BseBI CCWGG 1 cut(s) 1421
BseCI ATCGAT 2 cut(s) 531, 1203
BseDI CCNNGG 1 cut(s) 726
BseGI GGATG 3 cut(s) 85, 1337, 1429
BseLI CCNNNNNNNGG 4 cut(s) 396, 847, 1273, 1493
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 1 cut(s) 173
BseRI GAGGAG 3 cut(s) 32, 35, 1538
BseXI GCAGC 1 cut(s) 599
BseYI CCCAGC 1 cut(s) 867
Bsh1236I CGCG 4 cut(s) 74, 192, 380, 1292
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 3 cut(s) 449, 840, 851
BshNI GGYRCC 1 cut(s) 761
BshVI ATCGAT 2 cut(s) 531, 1203
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 2 cut(s) 798, 1317
BsiHKCI CYCGRG 1 cut(s) 1142
BsiSI CCGG 8 cut(s) 270, 363, 494, 545, 885, 1038, 1412, 1438
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 4 cut(s) 396, 847, 1273, 1493
BsmAI GTCTC 5 cut(s) 89, 221, 267, 663, 1501
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 3 cut(s) 449, 840, 851
Bso31I GGTCTC 2 cut(s) 221, 267
BsoBI CYCGRG 1 cut(s) 1142
Bsp1286I GDGCHC 2 cut(s) 798, 1317
BspACI CCGC 5 cut(s) 72, 572, 575, 584, 971
BspANI GGCC 3 cut(s) 449, 840, 851
BspCNI CTCAG 1 cut(s) 172
BspDI ATCGAT 2 cut(s) 531, 1203
BspFNI CGCG 4 cut(s) 74, 192, 380, 1292
BspLI GGNNCC 2 cut(s) 763, 1159
BspPI GGATC 4 cut(s) 556, 981, 1225, 1422
BspT107I GGYRCC 1 cut(s) 761
BspTNI GGTCTC 2 cut(s) 221, 267
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 884, 1037
BssAI RCCGGY 2 cut(s) 884, 1037
BssECI CCNNGG 1 cut(s) 726
BssNI GRCGYC 3 cut(s) 102, 267, 873
Bst2UI CCWGG 1 cut(s) 1421
BstACI GRCGYC 3 cut(s) 102, 267, 873
BstBAI YACGTR 2 cut(s) 1078, 1473
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 3 cut(s) 85, 1337, 1429
BstFNI CGCG 4 cut(s) 74, 192, 380, 1292
BstHHI GCGC 3 cut(s) 192, 382, 1292
BstMAI GTCTC 5 cut(s) 89, 221, 267, 663, 1501
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 5 cut(s) 386, 581, 584, 1298, 1525
BstNI CCWGG 1 cut(s) 1421
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 544, 1419
BstSNI TACGTA 2 cut(s) 1078, 1473
BstUI CGCG 4 cut(s) 74, 192, 380, 1292
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 2 cut(s) 55, 178
BstXI CCANNNNNNTGG 1 cut(s) 836
Bsu15I ATCGAT 2 cut(s) 531, 1203
BsuI GTATCC 1 cut(s) 1090
BsuRI GGCC 3 cut(s) 449, 840, 851
BsuTUI ATCGAT 2 cut(s) 531, 1203
BtsCI GGATG 3 cut(s) 85, 1337, 1429
BtsIMutI CAGTG 2 cut(s) 57, 468
CfoI GCGC 3 cut(s) 192, 382, 1292
Cfr10I RCCGGY 2 cut(s) 884, 1037
Cfr13I GGNCC 5 cut(s) 447, 849, 1256, 1365, 1409
ClaI ATCGAT 2 cut(s) 531, 1203
CpoI CGGWCCG 1 cut(s) 1365
CseI GACGC 2 cut(s) 275, 1281
Csp6I GTAC 8 cut(s) 181, 340, 676, 739, 1252, 1335, 1470, 1474
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
CspI CGGWCCG 1 cut(s) 1365
CviAII CATG 8 cut(s) 278, 324, 502, 644, 742, 820, 853, 907
CviQI GTAC 8 cut(s) 181, 340, 676, 739, 1252, 1335, 1470, 1474
DdeI CTNAG 1 cut(s) 159
DraI TTTAAA 1 cut(s) 1309
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
EciI GGCGGA 1 cut(s) 986
Eco105I TACGTA 2 cut(s) 1078, 1473
Eco31I GGTCTC 2 cut(s) 221, 267
Eco32I GATATC 2 cut(s) 260, 556
Eco47I GGWCC 3 cut(s) 1256, 1365, 1409
Eco88I CYCGRG 1 cut(s) 1142
EcoRII CCWGG 1 cut(s) 1419
EcoRV GATATC 2 cut(s) 260, 556
FaeI CATG 8 cut(s) 281, 327, 505, 647, 745, 823, 856, 910
FaqI GGGAC 1 cut(s) 884
FatI CATG 8 cut(s) 277, 323, 501, 643, 741, 819, 852, 906
FbaI TGATCA 3 cut(s) 54, 205, 1114
Fnu4HI GCNGC 5 cut(s) 72, 573, 576, 585, 588
FokI GGATG 3 cut(s) 92, 1344, 1436
Fsp4HI GCNGC 5 cut(s) 72, 573, 576, 585, 588
FspBI CTAG 3 cut(s) 833, 899, 1244
GlaI GCGC 3 cut(s) 191, 381, 1291
GluI GCNGC 5 cut(s) 72, 573, 576, 585, 588
GsaI CCCAGC 1 cut(s) 871
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 3 cut(s) 449, 840, 851
HapII CCGG 8 cut(s) 270, 363, 494, 545, 885, 1038, 1412, 1438
HgaI GACGC 2 cut(s) 275, 1281
HhaI GCGC 3 cut(s) 192, 382, 1292
Hin1I GRCGYC 3 cut(s) 102, 267, 873
Hin1II CATG 8 cut(s) 281, 327, 505, 647, 745, 823, 856, 910
Hin6I GCGC 3 cut(s) 190, 380, 1290
HinP1I GCGC 3 cut(s) 190, 380, 1290
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 3 cut(s) 13, 347, 697
HpaII CCGG 8 cut(s) 270, 363, 494, 545, 885, 1038, 1412, 1438
Hpy166II GTNNAC 2 cut(s) 817, 1043
Hpy188III TCNNGA 7 cut(s) 168, 230, 263, 526, 1142, 1151, 1169
Hpy8I GTNNAC 2 cut(s) 817, 1043
Hpy99I CGWCG 3 cut(s) 107, 269, 464
HpyAV CCTTC 3 cut(s) 59, 641, 1148
HpyCH4IV ACGT 7 cut(s) 102, 873, 1020, 1050, 1077, 1452, 1472
HpyCH4V TGCA 3 cut(s) 640, 1072, 1562
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 581, 584, 1298, 1525
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 7 cut(s) 102, 873, 1020, 1050, 1077, 1452, 1472
Hsp92I GRCGYC 3 cut(s) 102, 267, 873
Hsp92II CATG 8 cut(s) 281, 327, 505, 647, 745, 823, 856, 910
HspAI GCGC 3 cut(s) 190, 380, 1290
Ksp22I TGATCA 3 cut(s) 54, 205, 1114
LmnI GCTCC 2 cut(s) 1489, 1525
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 2 cut(s) 1097, 1167
MaeI CTAG 3 cut(s) 833, 899, 1244
MaeII ACGT 7 cut(s) 102, 873, 1020, 1050, 1077, 1452, 1472
MaeIII GTNAC 6 cut(s) 193, 310, 344, 464, 1148, 1162
MboII GAAGA 7 cut(s) 55, 81, 183, 932, 1550, 1553, 1556
MfeI CAATTG 1 cut(s) 653
MhlI GDGCHC 2 cut(s) 798, 1317
MluCI AATT 5 cut(s) 284, 653, 990, 1351, 1447
MlyI GAGTC 2 cut(s) 341, 706
MmeI TCCRAC 4 cut(s) 57, 85, 1078, 1384
MseI TTAA 2 cut(s) 282, 1308
MslI CAYNNNNRTG 1 cut(s) 1422
MspI CCGG 8 cut(s) 270, 363, 494, 545, 885, 1038, 1412, 1438
MspR9I CCNGG 2 cut(s) 546, 1421
MunI CAATTG 1 cut(s) 653
MvaI CCWGG 1 cut(s) 1421
MvnI CGCG 4 cut(s) 74, 192, 380, 1292
MwoI GCNNNNNNNGC 5 cut(s) 386, 581, 584, 1298, 1525
NciI CCSGG 1 cut(s) 546
NlaIII CATG 8 cut(s) 281, 327, 505, 647, 745, 823, 856, 910
NlaIV GGNNCC 2 cut(s) 763, 1159
NmuCI GTSAC 5 cut(s) 193, 310, 344, 464, 1148
PaeR7I CTCGAG 1 cut(s) 1142
PcsI WCGNNNNNNNCGW 3 cut(s) 1017, 1149, 1158
PfeI GAWTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 396
PfoI TCCNGGA 1 cut(s) 544
PkrI GCNGC 5 cut(s) 73, 574, 577, 586, 589
Ple19I CGATCG 1 cut(s) 597
PleI GAGTC 2 cut(s) 341, 705
PpsI GAGTC 2 cut(s) 341, 705
Ppu21I YACGTR 2 cut(s) 1078, 1473
PshAI GACNNNNGTC 1 cut(s) 101
PsiI TTATAA 1 cut(s) 1569
Psp6I CCWGG 1 cut(s) 1419
PspFI CCCAGC 1 cut(s) 867
PspGI CCWGG 1 cut(s) 1419
PspN4I GGNNCC 2 cut(s) 763, 1159
PspPI GGNCC 5 cut(s) 447, 849, 1256, 1365, 1409
PvuI CGATCG 1 cut(s) 597
RsaI GTAC 8 cut(s) 182, 341, 677, 740, 1253, 1336, 1471, 1475
RsaNI GTAC 8 cut(s) 181, 340, 676, 739, 1252, 1335, 1470, 1474
RseI CAYNNNNRTG 1 cut(s) 1422
Rsr2I CGGWCCG 1 cut(s) 1365
RsrII CGGWCCG 1 cut(s) 1365
SaqAI TTAA 2 cut(s) 282, 1308
SatI GCNGC 5 cut(s) 72, 573, 576, 585, 588
Sau96I GGNCC 5 cut(s) 447, 849, 1256, 1365, 1409
SchI GAGTC 2 cut(s) 341, 706
ScrFI CCNGG 2 cut(s) 546, 1421
SduI GDGCHC 2 cut(s) 798, 1317
SfaNI GCATC 2 cut(s) 1097, 1167
Sfr274I CTCGAG 1 cut(s) 1142
SgrAI CRCCGGYG 1 cut(s) 884
SinI GGWCC 3 cut(s) 1256, 1365, 1409
SlaI CTCGAG 1 cut(s) 1142
SmiMI CAYNNNNRTG 1 cut(s) 1422
SmlI CTYRAG 3 cut(s) 1142, 1338, 1520
SmoI CTYRAG 3 cut(s) 1142, 1338, 1520
SnaBI TACGTA 2 cut(s) 1078, 1473
Sse9I AATT 5 cut(s) 284, 653, 990, 1351, 1447
SsiI CCGC 5 cut(s) 72, 572, 575, 584, 971
SspMI CTAG 3 cut(s) 833, 899, 1244
StyD4I CCNGG 2 cut(s) 544, 1419
TaiI ACGT 7 cut(s) 105, 876, 1023, 1053, 1080, 1455, 1475
TaqI TCGA 6 cut(s) 531, 593, 778, 1143, 1203, 1285
TasI AATT 5 cut(s) 284, 653, 990, 1351, 1447
TatI WGTACW 2 cut(s) 675, 1334
TauI GCSGC 4 cut(s) 74, 575, 578, 587
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 2 cut(s) 282, 1308
Tru9I TTAA 2 cut(s) 282, 1308
TscAI CASTG 2 cut(s) 64, 475
TseFI GTSAC 5 cut(s) 193, 310, 344, 464, 1148
TseI GCWGC 1 cut(s) 587
Tsp45I GTSAC 5 cut(s) 193, 310, 344, 464, 1148
TspDTI ATGAA 3 cut(s) 26, 403, 1268
TspGWI ACGGA 4 cut(s) 168, 529, 593, 1475
TspRI CASTG 2 cut(s) 64, 475
Van91I CCANNNNNTGG 1 cut(s) 396
VpaK11BI GGWCC 3 cut(s) 1256, 1365, 1409
XapI RAATTY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 2 cut(s) 284, 441
XhoI CTCGAG 1 cut(s) 1142
XspI CTAG 3 cut(s) 833, 899, 1244
ZraI GACGTC 2 cut(s) 103, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.