pycom17g09750

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004610
Physical Location & Seq
Reverse (-)
7421614 .. 7425558
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g09750.2

Sequence Viewer

Length: 1077 bp
ATGACCACATCAAGGCAAGCTTACAAGATAGATGAGAAAAATCCCGGGTCGGATCTTGCCGGAGAGACCGCCGCTGCAATGGCGGCCGCGGCCTTAGTGTTCAAGAAAAGTAATCCACATTACTCTCACCTCCTCGTACACCATGCCCAGGAGTTGTTTGAGTTTGGGGACAAGTATAGGGAGAAGTACGATGGGAGCTTGGGAGTGGTGAAGAGCTACTATGCATCGGTGAGTGGGTTTAAGGACGAGTTGTTGTGGGCAGCTTTGTGGCTATACAAAGCCACCGACAATGAGGAGTATTTGAAGTATGTAATTAACAAGGCTCATAGCTTTGGTGGTATTGGTTGGGCAATTACAGAGTTCAGTTGGGACGTTAAGTACGCTGGCCTTCAACTCCTTGCGTCAAAGTTGCTTTTAGAAGAAAAACACATGAAACACTCTCACATACTGGAGTTGTATAAATCAAAAGCAGACTACTACATGTGCTCATGCCTAAACAAAAATACTGCCACCAAAAATGTGGAACGCACACCGGCAGGCCTACTGTACGTAAGGCAATGGAACAACATGCAGTACGTTTCGACAGCTTCATTCCTCCTAACAGTCTACTCAGACTTCCTCAAAACCTCGAACCGAAAGCTCAGCTGCCATGGCGGAACGGCAGGCCATGAAGAAGTCCTCAGCTTTGCTAAATCGCAGATAGATTATATTTTGGGCTCCAACCCCTTGAACATGAGCTACTTGGTGGGATATGGACCAAAATACCCTCAGAGGGTGCACCACAGAGGAGCGTCCGTGGAGTCATATCGGGAGAACAAAGGGTTCATTGGGTGCACACAAGGGTATGATAATTGGTACGGCAGGGAAGAGCCAAACCCTAATGTTTTGGTCGGGGCTTTGGTTGGAGGGCCTGATTGCAAAGATAATTTTGTGGACAGGAGGGATAATTATATGCAGACTGAGGCCTGCACTTACAATACATCACCATTGGTGGGGGTTTTTGCAAAGTTTTCAGAATTGGAGGTCCAAAAGAATGTGGATCAGGGGCAAAATTTGGATTTGGTTGCTTCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

359

Amino Acids

40.37

Weight (kDa)

6.86

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 606
AccII CGCG 1 cut(s) 89
AciI CCGC 6 cut(s) 69, 72, 83, 87, 89, 654
AclWI GGATC 2 cut(s) 60, 1047
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 1 cut(s) 1051
AfaI GTAC 6 cut(s) 138, 188, 380, 548, 575, 857
AfiI CCNNNNNNNGG 4 cut(s) 12, 148, 772, 992
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 4 cut(s) 103, 304, 392, 730
AjnI CCWGG 1 cut(s) 147
Alw21I GWGCWC 3 cut(s) 488, 780, 836
Alw26I GTCTC 1 cut(s) 59
Alw44I GTGCAC 2 cut(s) 776, 832
AlwI GGATC 2 cut(s) 60, 1047
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 7 cut(s) 84, 90, 385, 538, 664, 908, 963
ApaLI GTGCAC 2 cut(s) 776, 832
ApeKI GCWGC 3 cut(s) 74, 260, 645
ApoI RAATTY 1 cut(s) 1051
AspS9I GGNCC 3 cut(s) 755, 908, 1024
AsuC2I CCSGG 2 cut(s) 45, 46
AsuHPI GGTGA 4 cut(s) 119, 220, 241, 975
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 2 cut(s) 755, 1024
BaeGI GKGCMC 2 cut(s) 780, 836
BanII GRGCYC 1 cut(s) 719
Bbv12I GWGCWC 3 cut(s) 488, 780, 836
BbvCI CCTCAGC 1 cut(s) 680
BbvI GCAGC 3 cut(s) 61, 272, 632
BccI CCATC 1 cut(s) 185
BceAI ACGGC 2 cut(s) 675, 874
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 2 cut(s) 45, 46
BcoDI GTCTC 1 cut(s) 59
BisI GCNGC 7 cut(s) 72, 75, 84, 87, 90, 261, 646
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 7 cut(s) 73, 76, 85, 88, 91, 262, 647
Bme1390I CCNGG 3 cut(s) 45, 46, 149
Bme18I GGWCC 2 cut(s) 755, 1024
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 3 cut(s) 755, 908, 1024
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 3 cut(s) 45, 46, 149
BmsI GCATC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 680
Bpu1102I GCTNAGC 1 cut(s) 641
BpuMI CCSGG 2 cut(s) 45, 46
BsaAI YACGTR 1 cut(s) 550
BsaI GGTCTC 1 cut(s) 59
BsaJI CCNNGG 5 cut(s) 44, 87, 147, 649, 795
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 148, 772, 992
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 2 cut(s) 84, 563
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 5 cut(s) 44, 87, 147, 649, 795
BseLI CCNNNNNNNGG 4 cut(s) 12, 148, 772, 992
BseMI GCAATG 2 cut(s) 84, 563
BseMII CTCAG 5 cut(s) 624, 655, 694, 782, 951
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 3 cut(s) 122, 308, 801
BseSI GKGCMC 2 cut(s) 780, 836
BseX3I CGGCCG 1 cut(s) 84
BseXI GCAGC 3 cut(s) 61, 272, 632
BsgI GTGCAG 1 cut(s) 952
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 7 cut(s) 86, 92, 387, 540, 666, 910, 965
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 3 cut(s) 488, 780, 836
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 3 cut(s) 45, 60, 533
BslFI GGGAC 2 cut(s) 182, 383
BslI CCNNNNNNNGG 4 cut(s) 12, 148, 772, 992
BsmAI GTCTC 1 cut(s) 59
BsmFI GGGAC 2 cut(s) 182, 383
BsnI GGCC 7 cut(s) 86, 92, 387, 540, 666, 910, 965
Bso31I GGTCTC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 4 cut(s) 488, 719, 780, 836
Bsp143I GATC 2 cut(s) 52, 1039
Bsp1720I GCTNAGC 1 cut(s) 641
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 6 cut(s) 69, 72, 83, 87, 89, 654
BspANI GGCC 7 cut(s) 86, 92, 387, 540, 666, 910, 965
BspCNI CTCAG 5 cut(s) 623, 654, 693, 781, 952
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 718
BspPI GGATC 2 cut(s) 60, 1047
BspQI GCTCTTC 2 cut(s) 206, 861
BspTNI GGTCTC 1 cut(s) 59
BsrDI GCAATG 2 cut(s) 84, 563
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 1 cut(s) 453
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 5 cut(s) 44, 87, 147, 649, 795
BssMI GATC 2 cut(s) 52, 1039
BssT1I CCWWGG 1 cut(s) 649
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 2 cut(s) 546, 604
Bst6I CTCTTC 2 cut(s) 206, 861
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 5 cut(s) 18, 385, 538, 664, 967
BstDEI CTNAG 6 cut(s) 94, 610, 641, 680, 768, 960
BstDSI CCRYGG 3 cut(s) 87, 649, 795
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 2 cut(s) 55, 1042
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 2 cut(s) 52, 1039
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 4 cut(s) 80, 83, 89, 651
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 2 cut(s) 484, 571
BstSCI CCNGG 3 cut(s) 43, 44, 147
BstSLI GKGCMC 2 cut(s) 780, 836
BstSNI TACGTA 1 cut(s) 550
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 3 cut(s) 61, 272, 632
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 52
BstZI CGGCCG 1 cut(s) 84
BsuRI GGCC 7 cut(s) 86, 92, 387, 540, 666, 910, 965
BtgI CCRYGG 3 cut(s) 87, 649, 795
Cac8I GCNNGC 5 cut(s) 18, 385, 538, 664, 967
CciNI GCGGCCGC 1 cut(s) 84
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 3 cut(s) 755, 908, 1024
Cfr42I CCGCGG 1 cut(s) 90
Cfr9I CCCGGG 1 cut(s) 44
CseI GACGC 2 cut(s) 390, 780
Csp6I GTAC 6 cut(s) 137, 187, 379, 547, 574, 856
CviAII CATG 8 cut(s) 143, 430, 481, 489, 568, 650, 668, 733
CviQI GTAC 6 cut(s) 137, 187, 379, 547, 574, 856
DdeI CTNAG 6 cut(s) 94, 610, 641, 680, 768, 960
DpnI GATC 2 cut(s) 54, 1041
DpnII GATC 2 cut(s) 52, 1039
EaeI YGGCCR 1 cut(s) 84
EagI CGGCCG 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 206, 861
EarI CTCTTC 2 cut(s) 206, 861
EciI GGCGGA 1 cut(s) 669
EclXI CGGCCG 1 cut(s) 84
Eco105I TACGTA 1 cut(s) 550
Eco130I CCWWGG 1 cut(s) 649
Eco147I AGGCCT 2 cut(s) 540, 965
Eco24I GRGCYC 1 cut(s) 719
Eco31I GGTCTC 1 cut(s) 59
Eco47I GGWCC 2 cut(s) 755, 1024
Eco52I CGGCCG 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 649
EcoT22I ATGCAT 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 649
FaeI CATG 8 cut(s) 146, 433, 484, 492, 571, 653, 671, 736
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 2 cut(s) 182, 383
FatI CATG 8 cut(s) 142, 429, 480, 488, 567, 649, 667, 732
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 7 cut(s) 72, 75, 84, 87, 90, 261, 646
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 7 cut(s) 72, 75, 84, 87, 90, 261, 646
GluI GCNGC 7 cut(s) 72, 75, 84, 87, 90, 261, 646
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 7 cut(s) 86, 92, 387, 540, 666, 910, 965
HapII CCGG 3 cut(s) 45, 60, 533
HgaI GACGC 2 cut(s) 390, 780
Hin1II CATG 8 cut(s) 146, 433, 484, 492, 571, 653, 671, 736
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 1 cut(s) 800
HpaII CCGG 3 cut(s) 45, 60, 533
HphI GGTGA 4 cut(s) 119, 220, 241, 975
Hpy166II GTNNAC 5 cut(s) 139, 607, 778, 834, 934
Hpy188I TCNGA 4 cut(s) 52, 613, 771, 1015
Hpy188III TCNNGA 2 cut(s) 103, 809
Hpy8I GTNNAC 5 cut(s) 139, 607, 778, 834, 934
HpyAV CCTTC 1 cut(s) 398
HpyCH4III ACNGT 2 cut(s) 546, 604
HpyCH4IV ACGT 3 cut(s) 372, 549, 576
HpyCH4V TGCA 9 cut(s) 77, 224, 571, 778, 834, 918, 955, 969, 1004
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 83, 89, 651
HpyF3I CTNAG 6 cut(s) 94, 610, 641, 680, 768, 960
HpySE526I ACGT 3 cut(s) 372, 549, 576
Hsp92II CATG 8 cut(s) 146, 433, 484, 492, 571, 653, 671, 736
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 2 cut(s) 52, 1039
LguI GCTCTTC 2 cut(s) 206, 861
LmnI GCTCC 3 cut(s) 195, 722, 788
Lsp1109I GCAGC 3 cut(s) 61, 272, 632
LweI GCATC 1 cut(s) 233
MaeII ACGT 3 cut(s) 372, 549, 576
MalI GATC 2 cut(s) 54, 1041
MboI GATC 2 cut(s) 52, 1039
MboII GAAGA 4 cut(s) 223, 431, 683, 878
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 4 cut(s) 488, 719, 780, 836
MluCI AATT 7 cut(s) 312, 351, 850, 925, 946, 1016, 1051
MlyI GAGTC 1 cut(s) 809
MmeI TCCRAC 3 cut(s) 30, 744, 883
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 3 cut(s) 240, 315, 375
MspA1I CMGCKG 3 cut(s) 74, 89, 645
MspI CCGG 3 cut(s) 45, 60, 533
MspR9I CCNGG 3 cut(s) 45, 46, 149
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 4 cut(s) 80, 83, 89, 651
NciI CCSGG 2 cut(s) 45, 46
NcoI CCATGG 1 cut(s) 649
NdeII GATC 2 cut(s) 52, 1039
NlaIII CATG 8 cut(s) 146, 433, 484, 492, 571, 653, 671, 736
NlaIV GGNNCC 1 cut(s) 718
NotI GCGGCCGC 1 cut(s) 84
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 2 cut(s) 484, 571
PceI AGGCCT 2 cut(s) 540, 965
PciI ACATGT 1 cut(s) 480
PciSI GCTCTTC 2 cut(s) 206, 861
PkrI GCNGC 7 cut(s) 73, 76, 85, 88, 91, 262, 647
PleI GAGTC 1 cut(s) 808
PpsI GAGTC 1 cut(s) 808
Ppu21I YACGTR 1 cut(s) 550
PscI ACATGT 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 3 cut(s) 755, 908, 1024
PsrI GAACNNNNNNTAC 2 cut(s) 722, 754
PsuI RGATCY 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 645
RsaI GTAC 6 cut(s) 138, 188, 380, 548, 575, 857
RsaNI GTAC 6 cut(s) 137, 187, 379, 547, 574, 856
SacII CCGCGG 1 cut(s) 90
SapI GCTCTTC 2 cut(s) 206, 861
SaqAI TTAA 3 cut(s) 240, 315, 375
SatI GCNGC 7 cut(s) 72, 75, 84, 87, 90, 261, 646
Sau3AI GATC 2 cut(s) 52, 1039
Sau96I GGNCC 3 cut(s) 755, 908, 1024
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 3 cut(s) 45, 46, 149
SduI GDGCHC 4 cut(s) 488, 719, 780, 836
SfaNI GCATC 1 cut(s) 233
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 2 cut(s) 755, 1024
SmaI CCCGGG 1 cut(s) 46
SnaBI TACGTA 1 cut(s) 550
Sse9I AATT 7 cut(s) 312, 351, 850, 925, 946, 1016, 1051
SseBI AGGCCT 2 cut(s) 540, 965
SsiI CCGC 6 cut(s) 69, 72, 83, 87, 89, 654
StuI AGGCCT 2 cut(s) 540, 965
StyD4I CCNGG 3 cut(s) 43, 44, 147
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 2 cut(s) 546, 604
TaiI ACGT 3 cut(s) 375, 552, 579
TaqI TCGA 2 cut(s) 581, 629
TasI AATT 7 cut(s) 312, 351, 850, 925, 946, 1016, 1051
TauI GCSGC 4 cut(s) 74, 86, 89, 92
Tru1I TTAA 3 cut(s) 240, 315, 375
Tru9I TTAA 3 cut(s) 240, 315, 375
TseI GCWGC 3 cut(s) 74, 260, 645
TspDTI ATGAA 4 cut(s) 446, 579, 684, 814
TspGWI ACGGA 1 cut(s) 784
TspMI CCCGGG 1 cut(s) 44
VneI GTGCAC 2 cut(s) 776, 832
VpaK11BI GGWCC 2 cut(s) 755, 1024
XapI RAATTY 1 cut(s) 1051
XceI RCATGY 2 cut(s) 484, 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmaI CCCGGG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 606
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.