MD11G1211100.v1.1

Endoglucanase 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004610
Physical Location & Seq
Forward (+)
30866736 .. 30870847
4112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1211100.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGAAGCAAGGTATTGTCCTCCAATGGGTTTCTCTACTCTCCATTTTCGCTCTCTTTTCCTTGTCTCAGACTCAGTCCTTCGACTACGGCGAAGCTCTCTCCAAGAGTCTCCTCTACTTCGAGTCCCAACGTTCCGGCCGCTTGCCCCACAATCAACGGGTCACCTGGCGTCACCATTCCGGCCTTGACGATGGCCTAGAACAAGGGGTGGACTTGGTGGGAGGGTACTATGATGCAGGTGACAATGTGAAATTTGGTCTGCCAATGGCTTTTACCATAACAATGCTGTCTTGGGGTGTCATTGAGTACGGCAAACAAATAGAGGATGCTGGAGAGTACAATCATGCCTTAGAAGCTATCAAATGGGGGACTGATTATTTCATCAAGGCTCATACTCACCCAAATGTTTTGTGGGCTGAGGTGGGAGATGGATCGACGGATCACTACTGTTGGCAAAGGCCGGAGGACATGACAACATCTCGACAAGCCTACAAGGTCGATGAGAACCACCCCGGGTCAGATATTGCAGGTGAGACTGCGGCGGCAATGGCAGCGGCTGCCATTGTGTTTAGGAAAACCAACCCACATTACTCTGGCCTACTTTTACACCATGCACAACAGTTGTTTGAGTTTGGTGACAAATATAGAGGTAAATATGATGAGAGTATTGAAGTGGTGAAGGGGCACTACACATCTTGGAGTGGTTACATGGATGAGTTGCTCTGGGCAGCATTGTGGTTGTACACAGCCACACAGAATGAGGATTATTTGAACTATGCTTTGAACAATGCTCAGTCTCTTGGGGGCACCACTTGGGCCATCAAAGAGTTTAGCTGGGATGTTAAGTATGCTGGTGTTCAAATCATTGCTTCAATGTTGCTAAAGGAAGAGAGACACAAGCAACACATGCAAATACTGCAACAGTATCGTTCAAAAGCAGAGTTTTACATGTGTGCTTGCCTCGACAGAAACAACGTAGCCAACGTGCAACGCACCCCAGGAGGCGTGTTGTACATCCGCCAATGGAACAACATGCAATACGTCTCAAACGCAGCGTTTCTACTCACAACGTACTCTGATTATCTCCAAGCCACTCATGAGAATCTTACTTGCGACAAAGGACAGGTGAGCCCACAAGAAATCTTATCTTTTGCCAAATCACAGGTTGATTACATTCTGGGGTCCAATCCAATGGCCATGAGCTACTTAATTGGGTATGGACCAAAATACCCTCAAAGGGTGCACCACAGGGGAGCATCCATTGATTCGTATAAGAAGAACAAGGGTTTCATTGGGTGCACTCAGGGGTACGATAGCTGGTATGGGAGGCAGGGTCCAAACCCTAACGTGCTTGTTGGGGCACTGGTTGGTGGGCCCGATGGGAACGATGGATTCAGGGACGAGAGGTGGAATTACATGCAGACTGAGGCATGCACTTACAACACTGCAACATTAGTTGGGGTTCTTGCTAAATTGGATAGATTAAAAGGTGAGTGTATGGGGTTTAATCAATCATTAATAGCTTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

511

Amino Acids

57.55

Weight (kDa)

5.94

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 29 - 489 7.5e-169 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 227, 518
Acc36I ACCTGC 2 cut(s) 227, 518
AccB1I GGYRCC 1 cut(s) 806
AciI CCGC 5 cut(s) 139, 539, 542, 554, 1018
AclI AACGTT 1 cut(s) 130
AclWI GGATC 2 cut(s) 439, 447
AcoI YGGCCR 2 cut(s) 136, 1194
AcsI RAATTY 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 169
AfaI GTAC 7 cut(s) 227, 308, 338, 743, 1013, 1073, 1310
AfiI CCNNNNNNNGG 2 cut(s) 25, 1237
AflIII ACRYGT 1 cut(s) 948
AgsI TTSAA 7 cut(s) 671, 772, 784, 860, 873, 933, 1527
AjnI CCWGG 2 cut(s) 164, 997
AluBI AGCT 7 cut(s) 95, 356, 834, 1203, 1317, 1523, 1530
AluI AGCT 7 cut(s) 95, 356, 834, 1203, 1317, 1523, 1530
Alw21I GWGCWC 2 cut(s) 1245, 1301
Alw26I GTCTC 6 cut(s) 69, 113, 527, 801, 886, 1048
Alw44I GTGCAC 2 cut(s) 1241, 1297
AlwI GGATC 2 cut(s) 439, 447
AlwNI CAGNNNCTG 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 8 cut(s) 136, 181, 193, 458, 595, 816, 1194, 1373
ApaI GGGCCC 1 cut(s) 1377
ApaLI GTGCAC 2 cut(s) 1241, 1297
ApeKI GCWGC 4 cut(s) 551, 557, 728, 1052
ApoI RAATTY 1 cut(s) 251
AseI ATTAAT 1 cut(s) 1517
AspS9I GGNCC 6 cut(s) 816, 1182, 1220, 1334, 1373, 1374
AsuC2I CCSGG 2 cut(s) 513, 514
AsuHPI GGTGA 9 cut(s) 154, 164, 251, 389, 542, 647, 688, 1138, 1502
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 3 cut(s) 1182, 1220, 1334
BaeGI GKGCMC 6 cut(s) 687, 809, 1245, 1301, 1363, 1377
BalI TGGCCA 1 cut(s) 1196
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 2 cut(s) 1133, 1377
BarI GAAGNNNNNNTAC 2 cut(s) 671, 703
Bbv12I GWGCWC 2 cut(s) 1245, 1301
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 4 cut(s) 544, 563, 740, 1064
BccI CCATC 5 cut(s) 185, 422, 827, 1373, 1382
BceAI ACGGC 2 cut(s) 103, 325
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 2 cut(s) 166, 999
BcnI CCSGG 2 cut(s) 513, 514
BcoDI GTCTC 6 cut(s) 69, 113, 527, 801, 886, 1048
BfaI CTAG 2 cut(s) 197, 1531
BfuAI ACCTGC 2 cut(s) 227, 518
BisI GCNGC 8 cut(s) 139, 540, 543, 552, 555, 558, 729, 1053
BlsI GCNGC 8 cut(s) 140, 541, 544, 553, 556, 559, 730, 1054
Bme1390I CCNGG 4 cut(s) 166, 513, 514, 999
Bme18I GGWCC 3 cut(s) 1182, 1220, 1334
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 6 cut(s) 816, 1182, 1220, 1334, 1373, 1374
BmiI GGNNCC 4 cut(s) 808, 1183, 1335, 1375
BmrFI CCNGG 4 cut(s) 166, 513, 514, 999
BmsI GCATC 3 cut(s) 223, 316, 1265
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 417
BpuMI CCSGG 2 cut(s) 513, 514
BsaHI GRCGYC 1 cut(s) 169
BsaJI CCNNGG 3 cut(s) 511, 512, 997
BsaXI ACNNNNNCTCC 2 cut(s) 1318, 1348
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 1237
Bse1I ACTGG 1 cut(s) 1368
Bse3DI GCAATG 2 cut(s) 552, 864
BseBI CCWGG 2 cut(s) 166, 999
BseDI CCNNGG 3 cut(s) 511, 512, 997
BseGI GGATG 5 cut(s) 331, 718, 844, 1014, 1256
BseLI CCNNNNNNNGG 2 cut(s) 25, 1237
BseMI GCAATG 2 cut(s) 552, 864
BseMII CTCAG 6 cut(s) 80, 86, 408, 806, 1316, 1416
BseNI ACTGG 1 cut(s) 1368
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 6 cut(s) 687, 809, 1245, 1301, 1363, 1377
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 4 cut(s) 544, 563, 740, 1064
BseYI CCCAGC 1 cut(s) 834
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 8 cut(s) 138, 183, 195, 460, 597, 818, 1196, 1375
BshNI GGYRCC 1 cut(s) 806
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 2 cut(s) 1245, 1301
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 135, 180, 461, 513
BslFI GGGAC 3 cut(s) 109, 382, 1412
BslI CCNNNNNNNGG 2 cut(s) 25, 1237
BsmAI GTCTC 6 cut(s) 69, 113, 527, 801, 886, 1048
BsmBI CGTCTC 1 cut(s) 1048
BsmFI GGGAC 3 cut(s) 109, 382, 1412
BsnI GGCC 8 cut(s) 138, 183, 195, 460, 597, 818, 1196, 1375
BsoBI CYCGRG 1 cut(s) 512
Bsp120I GGGCCC 1 cut(s) 1373
Bsp1286I GDGCHC 7 cut(s) 687, 809, 1133, 1245, 1301, 1363, 1377
Bsp1407I TGTACA 2 cut(s) 741, 1011
Bsp143I GATC 2 cut(s) 431, 439
BspACI CCGC 5 cut(s) 139, 539, 542, 554, 1018
BspANI GGCC 8 cut(s) 138, 183, 195, 460, 597, 818, 1196, 1375
BspCNI CTCAG 6 cut(s) 79, 85, 409, 805, 1315, 1417
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 4 cut(s) 808, 1183, 1335, 1375
BspMI ACCTGC 2 cut(s) 227, 518
BspPI GGATC 2 cut(s) 439, 447
BspT107I GGYRCC 1 cut(s) 806
BsrDI GCAATG 2 cut(s) 552, 864
BsrGI TGTACA 2 cut(s) 741, 1011
BsrI ACTGG 1 cut(s) 1368
BssECI CCNNGG 3 cut(s) 511, 512, 997
BssMI GATC 2 cut(s) 431, 439
BssNI GRCGYC 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 166, 999
Bst4CI ACNGT 3 cut(s) 449, 621, 924
Bst6I CTCTTC 1 cut(s) 882
BstACI GRCGYC 1 cut(s) 169
BstAPI GCANNNNNTGC 3 cut(s) 557, 907, 916
BstAUI TGTACA 2 cut(s) 741, 1011
BstC8I GCNNGC 3 cut(s) 143, 958, 1432
BstDEI CTNAG 7 cut(s) 66, 72, 349, 417, 792, 1302, 1425
BstEII GGTNACC 1 cut(s) 160
BstF5I GGATG 5 cut(s) 331, 718, 844, 1014, 1256
BstKTI GATC 2 cut(s) 434, 442
BstMAI GTCTC 6 cut(s) 69, 113, 527, 801, 886, 1048
BstMBI GATC 2 cut(s) 431, 439
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 6 cut(s) 353, 548, 551, 557, 907, 916
BstNI CCWGG 2 cut(s) 166, 999
BstNSI RCATGY 5 cut(s) 910, 952, 1036, 1420, 1434
BstPI GGTNACC 1 cut(s) 160
BstSCI CCNGG 4 cut(s) 164, 511, 512, 997
BstSLI GKGCMC 6 cut(s) 687, 809, 1245, 1301, 1363, 1377
BstV1I GCAGC 4 cut(s) 544, 563, 740, 1064
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstZI CGGCCG 1 cut(s) 136
BsuRI GGCC 8 cut(s) 138, 183, 195, 460, 597, 818, 1196, 1375
BtsCI GGATG 5 cut(s) 331, 718, 844, 1014, 1256
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 2 cut(s) 1361, 1443
BveI ACCTGC 2 cut(s) 227, 518
Cac8I GCNNGC 3 cut(s) 143, 958, 1432
CaiI CAGNNNCTG 1 cut(s) 557
CciI TCATGA 1 cut(s) 1096
Cfr13I GGNCC 6 cut(s) 816, 1182, 1220, 1334, 1373, 1374
Cfr9I CCCGGG 1 cut(s) 512
CseI GACGC 1 cut(s) 158
Csp6I GTAC 7 cut(s) 226, 307, 337, 742, 1012, 1072, 1309
CviQI GTAC 7 cut(s) 226, 307, 337, 742, 1012, 1072, 1309
DdeI CTNAG 7 cut(s) 66, 72, 349, 417, 792, 1302, 1425
DpnI GATC 2 cut(s) 433, 441
DpnII GATC 2 cut(s) 431, 439
EaeI YGGCCR 2 cut(s) 136, 1194
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
EciI GGCGGA 1 cut(s) 1007
EclXI CGGCCG 1 cut(s) 136
Eco24I GRGCYC 2 cut(s) 1133, 1377
Eco47I GGWCC 3 cut(s) 1182, 1220, 1334
Eco52I CGGCCG 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 164, 997
EcoT38I GRGCYC 2 cut(s) 1133, 1377
Esp3I CGTCTC 1 cut(s) 1048
FaqI GGGAC 3 cut(s) 109, 382, 1412
Fnu4HI GCNGC 8 cut(s) 139, 540, 543, 552, 555, 558, 729, 1053
FokI GGATG 5 cut(s) 338, 725, 851, 1001, 1243
FriOI GRGCYC 2 cut(s) 1133, 1377
Fsp4HI GCNGC 8 cut(s) 139, 540, 543, 552, 555, 558, 729, 1053
FspBI CTAG 2 cut(s) 197, 1531
GluI GCNGC 8 cut(s) 139, 540, 543, 552, 555, 558, 729, 1053
GsaI CCCAGC 1 cut(s) 838
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 8 cut(s) 138, 183, 195, 460, 597, 818, 1196, 1375
HapII CCGG 4 cut(s) 135, 180, 461, 513
HgaI GACGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 169
HinfI GANTC 6 cut(s) 70, 106, 122, 1102, 1265, 1392
HpaII CCGG 4 cut(s) 135, 180, 461, 513
HphI GGTGA 9 cut(s) 154, 164, 251, 389, 542, 647, 688, 1138, 1502
Hpy166II GTNNAC 4 cut(s) 211, 744, 1243, 1299
Hpy188I TCNGA 3 cut(s) 69, 520, 1078
Hpy188III TCNNGA 2 cut(s) 480, 1097
Hpy8I GTNNAC 4 cut(s) 211, 744, 1243, 1299
Hpy99I CGWCG 1 cut(s) 439
HpyAV CCTTC 2 cut(s) 88, 673
HpyCH4III ACNGT 3 cut(s) 449, 621, 924
HpyCH4IV ACGT 6 cut(s) 130, 975, 984, 1041, 1070, 1347
HpyF10VI GCNNNNNNNGC 6 cut(s) 353, 548, 551, 557, 907, 916
HpyF3I CTNAG 7 cut(s) 66, 72, 349, 417, 792, 1302, 1425
HpySE526I ACGT 6 cut(s) 130, 975, 984, 1041, 1070, 1347
Hsp92I GRCGYC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 431, 439
LmnI GCTCC 1 cut(s) 1253
Lsp1109I GCAGC 4 cut(s) 544, 563, 740, 1064
LweI GCATC 3 cut(s) 223, 316, 1265
MaeI CTAG 2 cut(s) 197, 1531
MaeII ACGT 6 cut(s) 130, 975, 984, 1041, 1070, 1347
MaeIII GTNAC 5 cut(s) 160, 170, 239, 635, 704
MalI GATC 2 cut(s) 433, 441
MboI GATC 2 cut(s) 431, 439
MboII GAAGA 2 cut(s) 899, 1288
MhlI GDGCHC 7 cut(s) 687, 809, 1133, 1245, 1301, 1363, 1377
MlsI TGGCCA 1 cut(s) 1196
MluCI AATT 4 cut(s) 251, 1209, 1411, 1472
MluNI TGGCCA 1 cut(s) 1196
MlyI GAGTC 3 cut(s) 64, 115, 131
Mox20I TGGCCA 1 cut(s) 1196
MscI TGGCCA 1 cut(s) 1196
MseI TTAA 5 cut(s) 843, 1208, 1484, 1506, 1517
MslI CAYNNNNRTG 2 cut(s) 281, 402
Msp20I TGGCCA 1 cut(s) 1196
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 4 cut(s) 135, 180, 461, 513
MspR9I CCNGG 4 cut(s) 166, 513, 514, 999
MvaI CCWGG 2 cut(s) 166, 999
MwoI GCNNNNNNNGC 6 cut(s) 353, 548, 551, 557, 907, 916
NciI CCSGG 2 cut(s) 513, 514
NdeII GATC 2 cut(s) 431, 439
NlaIV GGNNCC 4 cut(s) 808, 1183, 1335, 1375
NmuCI GTSAC 4 cut(s) 160, 170, 239, 635
NspI RCATGY 5 cut(s) 910, 952, 1036, 1420, 1434
PaeI GCATGC 1 cut(s) 1434
PagI TCATGA 1 cut(s) 1096
PaqCI CACCTGC 2 cut(s) 227, 518
PciI ACATGT 1 cut(s) 948
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 981
PfeI GAWTC 3 cut(s) 1102, 1265, 1392
PflFI GACNNNGTC 1 cut(s) 73
PkrI GCNGC 8 cut(s) 140, 541, 544, 553, 556, 559, 730, 1054
PleI GAGTC 3 cut(s) 64, 114, 130
PpsI GAGTC 3 cut(s) 64, 114, 130
PscI ACATGT 1 cut(s) 948
PshBI ATTAAT 1 cut(s) 1517
Psp1406I AACGTT 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 164, 997
PspEI GGTNACC 1 cut(s) 160
PspFI CCCAGC 1 cut(s) 834
PspGI CCWGG 2 cut(s) 164, 997
PspN4I GGNNCC 4 cut(s) 808, 1183, 1335, 1375
PspOMI GGGCCC 1 cut(s) 1373
PspPI GGNCC 6 cut(s) 816, 1182, 1220, 1334, 1373, 1374
PstNI CAGNNNCTG 1 cut(s) 557
PsyI GACNNNGTC 1 cut(s) 73
RsaI GTAC 7 cut(s) 227, 308, 338, 743, 1013, 1073, 1310
RsaNI GTAC 7 cut(s) 226, 307, 337, 742, 1012, 1072, 1309
RseI CAYNNNNRTG 2 cut(s) 281, 402
SaqAI TTAA 5 cut(s) 843, 1208, 1484, 1506, 1517
SatI GCNGC 8 cut(s) 139, 540, 543, 552, 555, 558, 729, 1053
Sau3AI GATC 2 cut(s) 431, 439
Sau96I GGNCC 6 cut(s) 816, 1182, 1220, 1334, 1373, 1374
SchI GAGTC 3 cut(s) 64, 115, 131
ScrFI CCNGG 4 cut(s) 166, 513, 514, 999
SduI GDGCHC 7 cut(s) 687, 809, 1133, 1245, 1301, 1363, 1377
SfaNI GCATC 3 cut(s) 223, 316, 1265
SinI GGWCC 3 cut(s) 1182, 1220, 1334
SmaI CCCGGG 1 cut(s) 514
SmiMI CAYNNNNRTG 2 cut(s) 281, 402
SphI GCATGC 1 cut(s) 1434
Sse9I AATT 4 cut(s) 251, 1209, 1411, 1472
SsiI CCGC 5 cut(s) 139, 539, 542, 554, 1018
SspMI CTAG 2 cut(s) 197, 1531
StyD4I CCNGG 4 cut(s) 164, 511, 512, 997
TaaI ACNGT 3 cut(s) 449, 621, 924
TaiI ACGT 6 cut(s) 133, 978, 987, 1044, 1073, 1350
TaqI TCGA 6 cut(s) 81, 120, 434, 481, 498, 963
TasI AATT 4 cut(s) 251, 1209, 1411, 1472
TatI WGTACW 3 cut(s) 336, 741, 1011
TauI GCSGC 4 cut(s) 141, 542, 545, 557
TfiI GAWTC 3 cut(s) 1102, 1265, 1392
Tru1I TTAA 5 cut(s) 843, 1208, 1484, 1506, 1517
Tru9I TTAA 5 cut(s) 843, 1208, 1484, 1506, 1517
TscAI CASTG 2 cut(s) 1368, 1450
TseFI GTSAC 4 cut(s) 160, 170, 239, 635
TseI GCWGC 4 cut(s) 551, 557, 728, 1052
Tsp45I GTSAC 4 cut(s) 160, 170, 239, 635
TspDTI ATGAA 3 cut(s) 17, 370, 1279
TspGWI ACGGA 1 cut(s) 452
TspMI CCCGGG 1 cut(s) 512
TspRI CASTG 2 cut(s) 1368, 1450
Tth111I GACNNNGTC 1 cut(s) 73
VneI GTGCAC 2 cut(s) 1241, 1297
VpaK11BI GGWCC 3 cut(s) 1182, 1220, 1334
VspI ATTAAT 1 cut(s) 1517
XapI RAATTY 1 cut(s) 251
XceI RCATGY 5 cut(s) 910, 952, 1036, 1420, 1434
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XmaI CCCGGG 1 cut(s) 512
XspI CTAG 2 cut(s) 197, 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.