MD17G1103100.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004610
Physical Location & Seq
Reverse (-)
8762153 .. 8765994
3842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1103100.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGGAGGAGAAGAAGACACAACGGCAGCATCATCATCTTCTTCTTCTTCTACTCACCTTCAACATTTTCACCACCGCCAGTGCTTTCAATTACAGTGATGCCCTCACAAAGAGTCTCCTCTACTTCGAATCGCAACGCTCCGGCCGCCTGCCGTACAACCAGAGAGTCACCTGGCGTGACCATTCCGGCCTCACCGATGGCCTAGAGCAAGGGGTGGACTTGGTAGGAGGATATTACGATGCCGGCGACCACGTCAAGTTCGGACTGCCGATGGCATTTACAGTGACGATGCTGTCGTGGAGTGTTATAGAATACCGGCAGCAAATTGCGGCCGCCGGAGAATTGGAACATGCAATGGAGGCGATCAAATGGGGGACTGACTATTTCATCAAAGCTCACACTAGTCCCAATGTGTTATGGGCAGAGGTGGGTGATGGGGACACCGATCATTACTGCTGGCAACGGCCGGAGGACATGACCACATCAAGGCAAGCTTACAAGATAGATGAGAAAAATCCCGGGTCGGATCTTGCCGGAGAGACCGCCGCTGCAATGGCGGCCGCGGTCTTAGTGTTCAAGAAAAGTAACCCACATTACTCTCACCTCCTCCTACACCATGCCCAGCAGTTGTTTGAGTTTGGGGACAAGTATAGGGAGAAGTACGATGGGAGCTTGGGAGTGGTGAAGAGCTACTATGCGTCGGTGAGTGGGTTTAAGGACGAGTTGTTGTGGGCAGCTTTGTGGCTATACAAAGCCACCGACAATGAGGAGTATTTGAAGTATGTAATTAACAAGGCTCATAGCCTTGGTGGCATTGGTTGGGCAATTACAGAGTTCAGTTGGGACGTTAAGTACGCTGGCCTTCAACTCCTTGCGTCAAAGTTGCTTTTAGAAGAAGAACACATGAAACACTCTCACATACTAGAGTTGTATAAATCAAAAGCAGACTACTACATGTGCTCATGCCTAAACAAAAATACTGCAACCAAAAACGTGGAACGCACACCGGCAGGCCTACTGTACGTAAGGCAATGGAACAACATGCAGTACGTTTCAACAGCTTCGTTCCTCCTAACAGTCTACTCAGACTTCCTCGAAACCTCGAACCAAAAGCTCAGCTGCCATGGCGGAACGGCAGGCCATGAAGAAGTCCTCAGCTTTGCTAAATCGCAGATAGATTATATTTTGGGCTCCAACCCTTTGAACATGAGCTACTTGGTGGGATATGGACCAAAATACCCTCGGAGGGTGCACCACAGAGGAGCGTCCGTGGAATCATATCGGGAGAACAAAGGGTTCATTGGGTGCACACAAGGGTATGATAATTGGTACGGCAGGGAGGAGCCAAACCCTAATGTTTTGGTCGGGGCTTTGGTTGGAGGGCCTGATTGCAAAGATAATTTTGTGGACAGGAGGGATAATTACATGCAGACTGAGGCCTGCACTTACAATACAGCACCATTAGTGGGGGTTTTTGCAAAGTTTTCAGAATTGGAGGTCCAAAAGAATGTGGATCAGGGGCAAACTTTGGATTTGGTTGCTTCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

517

Amino Acids

58.45

Weight (kDa)

5.92

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 31 - 491 1e-167 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 1080
AccII CGCG 1 cut(s) 563
AclWI GGATC 2 cut(s) 534, 1521
AcoI YGGCCR 4 cut(s) 142, 330, 464, 558
AfaI GTAC 6 cut(s) 155, 662, 854, 1022, 1049, 1331
AfiI CCNNNNNNNGG 3 cut(s) 486, 1246, 1466
AflIII ACRYGT 1 cut(s) 954
AgsI TTSAA 7 cut(s) 61, 88, 577, 778, 866, 1056, 1204
AhlI ACTAGT 1 cut(s) 401
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 170
Alw21I GWGCWC 3 cut(s) 962, 1254, 1310
Alw26I GTCTC 2 cut(s) 119, 533
Alw44I GTGCAC 2 cut(s) 1250, 1306
AlwI GGATC 2 cut(s) 534, 1521
Ama87I CYCGRG 1 cut(s) 518
ApaLI GTGCAC 2 cut(s) 1250, 1306
ApeKI GCWGC 5 cut(s) 25, 319, 548, 734, 1119
AspS9I GGNCC 3 cut(s) 1229, 1382, 1498
AsuC2I CCSGG 2 cut(s) 519, 520
AsuHPI GGTGA 8 cut(s) 46, 61, 160, 184, 443, 593, 694, 715
AsuII TTCGAA 1 cut(s) 126
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 2 cut(s) 1229, 1498
BaeGI GKGCMC 2 cut(s) 1254, 1310
BanII GRGCYC 1 cut(s) 1193
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 3 cut(s) 962, 1254, 1310
BbvCI CCTCAGC 1 cut(s) 1154
BbvI GCAGC 5 cut(s) 37, 331, 535, 746, 1106
BccI CCATC 4 cut(s) 191, 265, 428, 659
BceAI ACGGC 5 cut(s) 38, 136, 479, 1149, 1348
BciT130I CCWGG 1 cut(s) 172
BcnI CCSGG 2 cut(s) 519, 520
BcoDI GTCTC 2 cut(s) 119, 533
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 3 cut(s) 203, 402, 923
BglI GCCNNNNNGGC 1 cut(s) 810
BlpI GCTNAGC 1 cut(s) 1115
Bme1390I CCNGG 3 cut(s) 172, 519, 520
Bme18I GGWCC 2 cut(s) 1229, 1498
BmeT110I CYCGRG 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 1229, 1382, 1498
BmiI GGNNCC 2 cut(s) 1192, 1344
BmrFI CCNGG 3 cut(s) 172, 519, 520
BmsI GCATC 4 cut(s) 37, 88, 229, 279
BpiI GAAGAC 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 1154
Bpu1102I GCTNAGC 1 cut(s) 1115
Bpu14I TTCGAA 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 519, 520
BsaAI YACGTR 1 cut(s) 1024
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 6 cut(s) 518, 561, 805, 1123, 1241, 1269
BsaXI ACNNNNNCTCC 2 cut(s) 1254, 1284
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 1246, 1466
Bse118I RCCGGY 3 cut(s) 242, 315, 1006
Bse1I ACTGG 1 cut(s) 78
Bse3DI GCAATG 3 cut(s) 360, 558, 1037
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 6 cut(s) 518, 561, 805, 1123, 1241, 1269
BseLI CCNNNNNNNGG 3 cut(s) 486, 1246, 1466
BseMI GCAATG 3 cut(s) 360, 558, 1037
BseMII CTCAG 4 cut(s) 1098, 1129, 1168, 1425
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 6 cut(s) 20, 107, 596, 782, 1275, 1355
BseSI GKGCMC 2 cut(s) 1254, 1310
BseX3I CGGCCG 4 cut(s) 142, 330, 464, 558
BseXI GCAGC 5 cut(s) 37, 331, 535, 746, 1106
BseYI CCCAGC 1 cut(s) 621
BsgI GTGCAG 1 cut(s) 1426
Bsh1236I CGCG 1 cut(s) 563
Bsh1285I CGRYCG 4 cut(s) 145, 333, 467, 561
BsiEI CGRYCG 4 cut(s) 145, 333, 467, 561
BsiHKAI GWGCWC 3 cut(s) 962, 1254, 1310
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 9 cut(s) 141, 186, 243, 316, 336, 467, 519, 534, 1007
BslFI GGGAC 5 cut(s) 388, 390, 452, 656, 857
BslI CCNNNNNNNGG 3 cut(s) 486, 1246, 1466
BsmAI GTCTC 2 cut(s) 119, 533
BsmFI GGGAC 5 cut(s) 388, 390, 452, 656, 857
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 1 cut(s) 518
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 4 cut(s) 962, 1193, 1254, 1310
Bsp143I GATC 4 cut(s) 363, 445, 526, 1513
Bsp1720I GCTNAGC 1 cut(s) 1115
Bsp19I CCATGG 1 cut(s) 1123
BspCNI CTCAG 4 cut(s) 1097, 1128, 1167, 1426
BspFNI CGCG 1 cut(s) 563
BspLI GGNNCC 2 cut(s) 1192, 1344
BspPI GGATC 2 cut(s) 534, 1521
BspQI GCTCTTC 1 cut(s) 680
BspT104I TTCGAA 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 3 cut(s) 360, 558, 1037
BsrFI RCCGGY 3 cut(s) 242, 315, 1006
BsrI ACTGG 1 cut(s) 78
BssAI RCCGGY 3 cut(s) 242, 315, 1006
BssECI CCNNGG 6 cut(s) 518, 561, 805, 1123, 1241, 1269
BssMI GATC 4 cut(s) 363, 445, 526, 1513
BssT1I CCWWGG 2 cut(s) 805, 1123
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 4 cut(s) 95, 283, 1020, 1078
Bst6I CTCTTC 1 cut(s) 680
BstBAI YACGTR 1 cut(s) 1024
BstBI TTCGAA 1 cut(s) 126
BstC8I GCNNGC 8 cut(s) 149, 244, 458, 492, 859, 1012, 1138, 1441
BstDEI CTNAG 5 cut(s) 568, 1084, 1115, 1154, 1434
BstDSI CCRYGG 3 cut(s) 561, 1123, 1269
BstFNI CGCG 1 cut(s) 563
BstKTI GATC 4 cut(s) 366, 448, 529, 1516
BstMAI GTCTC 2 cut(s) 119, 533
BstMBI GATC 4 cut(s) 363, 445, 526, 1513
BstMCI CGRYCG 4 cut(s) 145, 333, 467, 561
BstMWI GCNNNNNNNGC 6 cut(s) 144, 359, 554, 557, 810, 1125
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 4 cut(s) 353, 958, 1045, 1429
BstSCI CCNGG 3 cut(s) 170, 517, 518
BstSLI GKGCMC 2 cut(s) 1254, 1310
BstSNI TACGTA 1 cut(s) 1024
BstUI CGCG 1 cut(s) 563
BstV1I GCAGC 5 cut(s) 37, 331, 535, 746, 1106
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 526
BstXI CCANNNNNNTGG 1 cut(s) 994
BstYI RGATCY 1 cut(s) 526
BstZI CGGCCG 4 cut(s) 142, 330, 464, 558
BtgI CCRYGG 3 cut(s) 561, 1123, 1269
BtrI CACGTC 1 cut(s) 253
BtsIMutI CAGTG 3 cut(s) 85, 100, 288
Cac8I GCNNGC 8 cut(s) 149, 244, 458, 492, 859, 1012, 1138, 1441
CciNI GCGGCCGC 2 cut(s) 330, 558
Cfr10I RCCGGY 3 cut(s) 242, 315, 1006
Cfr13I GGNCC 3 cut(s) 1229, 1382, 1498
Cfr42I CCGCGG 1 cut(s) 564
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 3 cut(s) 687, 864, 1254
Csp6I GTAC 6 cut(s) 154, 661, 853, 1021, 1048, 1330
CviQI GTAC 6 cut(s) 154, 661, 853, 1021, 1048, 1330
DdeI CTNAG 5 cut(s) 568, 1084, 1115, 1154, 1434
DpnI GATC 4 cut(s) 365, 447, 528, 1515
DpnII GATC 4 cut(s) 363, 445, 526, 1513
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 4 cut(s) 142, 330, 464, 558
EagI CGGCCG 4 cut(s) 142, 330, 464, 558
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
EciI GGCGGA 1 cut(s) 1143
EclXI CGGCCG 4 cut(s) 142, 330, 464, 558
Eco105I TACGTA 1 cut(s) 1024
Eco130I CCWWGG 2 cut(s) 805, 1123
Eco147I AGGCCT 2 cut(s) 1014, 1439
Eco24I GRGCYC 1 cut(s) 1193
Eco31I GGTCTC 1 cut(s) 533
Eco47I GGWCC 2 cut(s) 1229, 1498
Eco52I CGGCCG 4 cut(s) 142, 330, 464, 558
Eco88I CYCGRG 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 1382
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 2 cut(s) 805, 1123
EcoT38I GRGCYC 1 cut(s) 1193
ErhI CCWWGG 2 cut(s) 805, 1123
FalI AAGNNNNNCTT 2 cut(s) 478, 510
FaqI GGGAC 5 cut(s) 388, 390, 452, 656, 857
FblI GTMKAC 1 cut(s) 1080
FriOI GRGCYC 1 cut(s) 1193
FspBI CTAG 3 cut(s) 203, 402, 923
GsaI CCCAGC 1 cut(s) 625
HapII CCGG 9 cut(s) 141, 186, 243, 316, 336, 467, 519, 534, 1007
HgaI GACGC 3 cut(s) 687, 864, 1254
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 4 cut(s) 112, 128, 165, 1274
HpaII CCGG 9 cut(s) 141, 186, 243, 316, 336, 467, 519, 534, 1007
HphI GGTGA 8 cut(s) 46, 61, 160, 184, 443, 593, 694, 715
Hpy166II GTNNAC 5 cut(s) 217, 1081, 1252, 1308, 1408
Hpy188I TCNGA 5 cut(s) 263, 526, 1087, 1245, 1489
Hpy188III TCNNGA 2 cut(s) 577, 1283
Hpy8I GTNNAC 5 cut(s) 217, 1081, 1252, 1308, 1408
Hpy99I CGWCG 1 cut(s) 703
HpyAV CCTTC 2 cut(s) 67, 872
HpyCH4III ACNGT 4 cut(s) 95, 283, 1020, 1078
HpyCH4IV ACGT 5 cut(s) 252, 846, 993, 1023, 1050
HpyF10VI GCNNNNNNNGC 6 cut(s) 144, 359, 554, 557, 810, 1125
HpyF3I CTNAG 5 cut(s) 568, 1084, 1115, 1154, 1434
HpySE526I ACGT 5 cut(s) 252, 846, 993, 1023, 1050
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
KspI CCGCGG 1 cut(s) 564
Kzo9I GATC 4 cut(s) 363, 445, 526, 1513
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 5 cut(s) 143, 669, 1196, 1262, 1342
Lsp1109I GCAGC 5 cut(s) 37, 331, 535, 746, 1106
LweI GCATC 4 cut(s) 37, 88, 229, 279
MaeI CTAG 3 cut(s) 203, 402, 923
MaeII ACGT 5 cut(s) 252, 846, 993, 1023, 1050
MaeIII GTNAC 4 cut(s) 166, 176, 283, 584
MalI GATC 4 cut(s) 365, 447, 528, 1515
MboI GATC 4 cut(s) 363, 445, 526, 1513
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 4 cut(s) 962, 1193, 1254, 1310
MluCI AATT 9 cut(s) 88, 324, 341, 786, 825, 1324, 1399, 1420, 1490
MlyI GAGTC 2 cut(s) 121, 174
MmeI TCCRAC 3 cut(s) 504, 1218, 1357
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 3 cut(s) 714, 789, 849
MspA1I CMGCKG 3 cut(s) 548, 563, 1119
MspI CCGG 9 cut(s) 141, 186, 243, 316, 336, 467, 519, 534, 1007
MspR9I CCNGG 3 cut(s) 172, 519, 520
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 1 cut(s) 563
MwoI GCNNNNNNNGC 6 cut(s) 144, 359, 554, 557, 810, 1125
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 2 cut(s) 519, 520
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 4 cut(s) 363, 445, 526, 1513
NgoMIV GCCGGC 1 cut(s) 242
NlaIV GGNNCC 2 cut(s) 1192, 1344
NmuCI GTSAC 3 cut(s) 166, 176, 283
NotI GCGGCCGC 2 cut(s) 330, 558
NspI RCATGY 4 cut(s) 353, 958, 1045, 1429
NspV TTCGAA 1 cut(s) 126
PceI AGGCCT 2 cut(s) 1014, 1439
PciI ACATGT 1 cut(s) 954
PciSI GCTCTTC 1 cut(s) 680
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 293
PdiI GCCGGC 1 cut(s) 244
PfeI GAWTC 2 cut(s) 128, 1274
PflFI GACNNNGTC 1 cut(s) 251
PleI GAGTC 2 cut(s) 120, 173
PpsI GAGTC 2 cut(s) 120, 173
Ppu21I YACGTR 1 cut(s) 1024
PscI ACATGT 1 cut(s) 954
Psp6I CCWGG 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 621
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 2 cut(s) 1192, 1344
PspPI GGNCC 3 cut(s) 1229, 1382, 1498
PsrI GAACNNNNNNTAC 2 cut(s) 1196, 1228
PsuI RGATCY 1 cut(s) 526
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 1119
RsaI GTAC 6 cut(s) 155, 662, 854, 1022, 1049, 1331
RsaNI GTAC 6 cut(s) 154, 661, 853, 1021, 1048, 1330
SacII CCGCGG 1 cut(s) 564
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 714, 789, 849
Sau3AI GATC 4 cut(s) 363, 445, 526, 1513
Sau96I GGNCC 3 cut(s) 1229, 1382, 1498
SchI GAGTC 2 cut(s) 121, 174
ScrFI CCNGG 3 cut(s) 172, 519, 520
SduI GDGCHC 4 cut(s) 962, 1193, 1254, 1310
SfaNI GCATC 4 cut(s) 37, 88, 229, 279
Sfr303I CCGCGG 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 126
SgrBI CCGCGG 1 cut(s) 564
SinI GGWCC 2 cut(s) 1229, 1498
SmaI CCCGGG 1 cut(s) 520
SnaBI TACGTA 1 cut(s) 1024
SpeI ACTAGT 1 cut(s) 401
Sse9I AATT 9 cut(s) 88, 324, 341, 786, 825, 1324, 1399, 1420, 1490
SseBI AGGCCT 2 cut(s) 1014, 1439
SspMI CTAG 3 cut(s) 203, 402, 923
StuI AGGCCT 2 cut(s) 1014, 1439
StyD4I CCNGG 3 cut(s) 170, 517, 518
StyI CCWWGG 2 cut(s) 805, 1123
TaaI ACNGT 4 cut(s) 95, 283, 1020, 1078
TaiI ACGT 5 cut(s) 255, 849, 996, 1026, 1053
TaqI TCGA 3 cut(s) 126, 1095, 1103
TasI AATT 9 cut(s) 88, 324, 341, 786, 825, 1324, 1399, 1420, 1490
TauI GCSGC 6 cut(s) 147, 332, 335, 548, 560, 563
TfiI GAWTC 2 cut(s) 128, 1274
Tru1I TTAA 3 cut(s) 714, 789, 849
Tru9I TTAA 3 cut(s) 714, 789, 849
TscAI CASTG 3 cut(s) 85, 100, 288
TseFI GTSAC 3 cut(s) 166, 176, 283
TseI GCWGC 5 cut(s) 25, 319, 548, 734, 1119
Tsp45I GTSAC 3 cut(s) 166, 176, 283
TspDTI ATGAA 4 cut(s) 376, 920, 1158, 1288
TspGWI ACGGA 1 cut(s) 1258
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 3 cut(s) 85, 100, 288
Tth111I GACNNNGTC 1 cut(s) 251
VneI GTGCAC 2 cut(s) 1250, 1306
VpaK11BI GGWCC 2 cut(s) 1229, 1498
XceI RCATGY 4 cut(s) 353, 958, 1045, 1429
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 1080
XspI CTAG 3 cut(s) 203, 402, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.