MD09G1113100.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004610
Physical Location & Seq
Reverse (-)
8584405 .. 8590382
5978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1113100.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGGAGGAGAAGAAGAAGAAGACACAATGGCATAATCTTCTTCTTCTTCTACTCACCCTCAACATTTTCACCACCGCCGGAGCTTTCAATTACAGTGATGCCCTCACAAAGAGTCTCCTATACTTCGAATCACAACGCTCCGGCCGCCTGCCGTACAACCAGAGAGTCACCTGGCGCGGCCATTCCGGCCTCACCGATGGCCTAGAGCAAGGGGTGGACTTGGTAGGAGGATATTATGATGCCGGCGATCACGTCAAGTTCGGATTGCCGATGGCATTTACAGTGACGATGCTGTCCTGGAGTGTTATAGAATACCGGCAGCAGATTGCGGCCGCCGGAGAATTGGAACATGCAATGGAGGCGATCAAATGGGGGACTGATTATTTCATCAAAGCTCACACTAGTCCCAATGTGTTATGGGCAGAGGTTGGTGATGGGGACACTGATCATTACTGCTGGCAACGGTCGGAGGACATGACGACATCAAGGCGAGCTTACAAAATAGACGAGAAAAATCCCGGGTCGGACCTTGCCGGAGAGAGCGCCGCTGCAATGGCGGCCGCGGCCTTGGTGTTCAAGGAAAGTAATCCACATTATTCCCACCTCCTCCTACACCACGCCCAACAGTTGTTTGAGTTTGGGGACAATTATAGGGAGAAGTATGATGGGAGCTTGGAAGTGGTGAAGAGCTACTATGCGTCGGTGAGTGGGTTTAAGGACGAGTTGCTGTGGGCAGCTTTGTGGCTGTACAAAGCCACCGACAATGAGGAATATTTGAAGTATGTAATTAACAAGGCTAATAGCTTTGGTGGCATTGGTTGGGCAATGACAGAGTTCAGTTGGGATGTTAAGTTCGCTGGCCTTCAACTCCTTGCTTCCAAGTTGCTTTTAGAAGAAAAACACAAGAAACACTCTCACATACTAGAGCTATATAAATCAAAAGCAGACTACTATATGTGCTCATGCCTAAACAAAAATACTGCGGCCAAAAATGTGGAACTCACACCGGCAGGCCTTCTGTACATAAGGCAATGGAATAACATGCAGTACGTTTCAGCAGCGTCATTCCTCCTAACAGTCTACTCAGACTTCCTCAAAACCTCGAACGGAAATCTCAATTGCCATGGTGGAACGGCGGGCCATGAAGAAGTCCTCAGCTTTGCTAAATCACAGATAGATTATATTTTGGGCTCCAACCCTTTGAACATGAGCTACTTGGTGGGATATGGACCAAAATACCCTCAGAGGGTGCACCATAGAGGAGCATCCGTGGAGTCGTATAGGGAGAACAAAGGGTTCATTGGGTGCACCCAGGGGTATGACAGTTGGTACGCCAGGGAGGAGCCAAACCCTAATGTTTTGGTCGGGGCTTTGGTTGGAGGGCCTGATTGCAAAGATAATTTTGTGGACAGGAGGGATAATTATATGCAGACTGAGGCTTGCACTTATAATACAGCACCATTGGTGGGGGTTTTTGCAAAGTTTTCAGACCAAGAGAATATGGATCAGGGGCAAAATTTGGATTTCGTTGCTTCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

514

Amino Acids

57.9

Weight (kDa)

5.8

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 31 - 491 2.9e-166 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1449
AasI GACNNNNNNGTC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 1080
AccII CGCG 2 cut(s) 177, 563
AclWI GGATC 1 cut(s) 1512
AcoI YGGCCR 5 cut(s) 142, 178, 330, 558, 984
AcsI RAATTY 1 cut(s) 1516
AfaI GTAC 5 cut(s) 155, 749, 1022, 1049, 1331
AfiI CCNNNNNNNGG 2 cut(s) 1246, 1466
AgsI TTSAA 5 cut(s) 88, 577, 778, 866, 1204
AhlI ACTAGT 1 cut(s) 401
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 4 cut(s) 170, 296, 1311, 1334
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
Alw21I GWGCWC 3 cut(s) 962, 1254, 1310
Alw26I GTCTC 1 cut(s) 119
Alw44I GTGCAC 2 cut(s) 1250, 1306
AlwI GGATC 1 cut(s) 1512
Ama87I CYCGRG 1 cut(s) 518
ApaLI GTGCAC 2 cut(s) 1250, 1306
ApeKI GCWGC 4 cut(s) 319, 548, 734, 1058
ApoI RAATTY 1 cut(s) 1516
AspLEI GCGC 2 cut(s) 177, 545
AspS9I GGNCC 4 cut(s) 526, 1138, 1229, 1382
AsuC2I CCSGG 2 cut(s) 519, 520
AsuHPI GGTGA 7 cut(s) 46, 61, 160, 184, 443, 694, 715
AsuII TTCGAA 1 cut(s) 126
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 2 cut(s) 526, 1229
BaeGI GKGCMC 2 cut(s) 1254, 1310
BanII GRGCYC 1 cut(s) 1193
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 3 cut(s) 962, 1254, 1310
BbvCI CCTCAGC 1 cut(s) 1154
BbvI GCAGC 4 cut(s) 331, 535, 746, 1070
BccI CCATC 4 cut(s) 191, 265, 428, 659
BceAI ACGGC 2 cut(s) 136, 1149
BciT130I CCWGG 4 cut(s) 172, 298, 1313, 1336
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 2 cut(s) 519, 520
BcoDI GTCTC 1 cut(s) 119
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 3 cut(s) 203, 402, 923
BfoI RGCGCY 1 cut(s) 546
BglI GCCNNNNNGGC 1 cut(s) 186
Bme1390I CCNGG 6 cut(s) 172, 298, 519, 520, 1313, 1336
Bme18I GGWCC 2 cut(s) 526, 1229
BmeT110I CYCGRG 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 4 cut(s) 526, 1138, 1229, 1382
BmiI GGNNCC 2 cut(s) 1192, 1344
BmrFI CCNGG 6 cut(s) 172, 298, 519, 520, 1313, 1336
BmsI GCATC 4 cut(s) 88, 229, 279, 1274
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 1154
Bpu14I TTCGAA 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 519, 520
BsaJI CCNNGG 8 cut(s) 518, 561, 567, 1123, 1269, 1311, 1312, 1335
BsaXI ACNNNNNCTCC 2 cut(s) 1254, 1284
Bsc4I CCNNNNNNNGG 2 cut(s) 1246, 1466
Bse118I RCCGGY 3 cut(s) 242, 315, 1006
Bse3DI GCAATG 4 cut(s) 360, 558, 831, 1037
BseBI CCWGG 4 cut(s) 172, 298, 1313, 1336
BseDI CCNNGG 8 cut(s) 518, 561, 567, 1123, 1269, 1311, 1312, 1335
BseGI GGATG 2 cut(s) 850, 1265
BseLI CCNNNNNNNGG 2 cut(s) 1246, 1466
BseMI GCAATG 4 cut(s) 360, 558, 831, 1037
BseMII CTCAG 4 cut(s) 1098, 1168, 1256, 1425
BseRI GAGGAG 4 cut(s) 20, 596, 1275, 1355
BseSI GKGCMC 2 cut(s) 1254, 1310
BseX3I CGGCCG 3 cut(s) 142, 330, 558
BseXI GCAGC 4 cut(s) 331, 535, 746, 1070
Bsh1236I CGCG 2 cut(s) 177, 563
Bsh1285I CGRYCG 4 cut(s) 145, 333, 467, 561
BsiEI CGRYCG 4 cut(s) 145, 333, 467, 561
BsiHKAI GWGCWC 3 cut(s) 962, 1254, 1310
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 9 cut(s) 78, 141, 186, 243, 316, 336, 519, 534, 1007
BslFI GGGAC 4 cut(s) 388, 390, 452, 656
BslI CCNNNNNNNGG 2 cut(s) 1246, 1466
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 4 cut(s) 388, 390, 452, 656
BsoBI CYCGRG 1 cut(s) 518
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 4 cut(s) 962, 1193, 1254, 1310
Bsp1407I TGTACA 2 cut(s) 747, 1020
Bsp143I GATC 4 cut(s) 247, 363, 445, 1504
Bsp19I CCATGG 1 cut(s) 1123
BspCNI CTCAG 4 cut(s) 1097, 1167, 1255, 1426
BspFNI CGCG 2 cut(s) 177, 563
BspLI GGNNCC 2 cut(s) 1192, 1344
BspPI GGATC 1 cut(s) 1512
BspQI GCTCTTC 1 cut(s) 680
BspT104I TTCGAA 1 cut(s) 126
BsrDI GCAATG 4 cut(s) 360, 558, 831, 1037
BsrFI RCCGGY 3 cut(s) 242, 315, 1006
BsrGI TGTACA 2 cut(s) 747, 1020
BssAI RCCGGY 3 cut(s) 242, 315, 1006
BssECI CCNNGG 8 cut(s) 518, 561, 567, 1123, 1269, 1311, 1312, 1335
BssMI GATC 4 cut(s) 247, 363, 445, 1504
BssT1I CCWWGG 2 cut(s) 567, 1123
Bst2UI CCWGG 4 cut(s) 172, 298, 1313, 1336
Bst4CI ACNGT 6 cut(s) 95, 283, 465, 627, 1078, 1325
Bst6I CTCTTC 1 cut(s) 680
BstAUI TGTACA 2 cut(s) 747, 1020
BstBI TTCGAA 1 cut(s) 126
BstC8I GCNNGC 8 cut(s) 149, 244, 458, 492, 859, 1012, 1138, 1441
BstDEI CTNAG 4 cut(s) 1084, 1154, 1242, 1434
BstDSI CCRYGG 3 cut(s) 561, 1123, 1269
BstF5I GGATG 2 cut(s) 850, 1265
BstFNI CGCG 2 cut(s) 177, 563
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 2 cut(s) 177, 545
BstKTI GATC 4 cut(s) 250, 366, 448, 1507
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 4 cut(s) 247, 363, 445, 1504
BstMCI CGRYCG 4 cut(s) 145, 333, 467, 561
BstMWI GCNNNNNNNGC 7 cut(s) 144, 186, 359, 554, 557, 563, 810
BstNI CCWGG 4 cut(s) 172, 298, 1313, 1336
BstNSI RCATGY 2 cut(s) 353, 1045
BstSCI CCNGG 6 cut(s) 170, 296, 517, 518, 1311, 1334
BstSLI GKGCMC 2 cut(s) 1254, 1310
BstUI CGCG 2 cut(s) 177, 563
BstV1I GCAGC 4 cut(s) 331, 535, 746, 1070
BstV2I GAAGAC 1 cut(s) 26
BstXI CCANNNNNNTGG 1 cut(s) 994
BstZI CGGCCG 3 cut(s) 142, 330, 558
BtgI CCRYGG 3 cut(s) 561, 1123, 1269
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 2 cut(s) 850, 1265
BtsIMutI CAGTG 3 cut(s) 100, 288, 441
Cac8I GCNNGC 8 cut(s) 149, 244, 458, 492, 859, 1012, 1138, 1441
CciNI GCGGCCGC 2 cut(s) 330, 558
CfoI GCGC 2 cut(s) 177, 545
Cfr10I RCCGGY 3 cut(s) 242, 315, 1006
Cfr13I GGNCC 4 cut(s) 526, 1138, 1229, 1382
Cfr42I CCGCGG 1 cut(s) 564
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 2 cut(s) 687, 1050
Csp6I GTAC 5 cut(s) 154, 748, 1021, 1048, 1330
CviAII CATG 7 cut(s) 350, 475, 963, 1042, 1124, 1142, 1207
CviQI GTAC 5 cut(s) 154, 748, 1021, 1048, 1330
DdeI CTNAG 4 cut(s) 1084, 1154, 1242, 1434
DpnI GATC 4 cut(s) 249, 365, 447, 1506
DpnII GATC 4 cut(s) 247, 363, 445, 1504
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 5 cut(s) 142, 178, 330, 558, 984
EagI CGGCCG 3 cut(s) 142, 330, 558
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
EclXI CGGCCG 3 cut(s) 142, 330, 558
Eco130I CCWWGG 2 cut(s) 567, 1123
Eco147I AGGCCT 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 1193
Eco47I GGWCC 2 cut(s) 526, 1229
Eco52I CGGCCG 3 cut(s) 142, 330, 558
Eco88I CYCGRG 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 1382
EcoRII CCWGG 4 cut(s) 170, 296, 1311, 1334
EcoT14I CCWWGG 2 cut(s) 567, 1123
EcoT38I GRGCYC 1 cut(s) 1193
ErhI CCWWGG 2 cut(s) 567, 1123
FaeI CATG 7 cut(s) 353, 478, 966, 1045, 1127, 1145, 1210
FalI AAGNNNNNCTT 2 cut(s) 478, 510
FaqI GGGAC 4 cut(s) 388, 390, 452, 656
FatI CATG 7 cut(s) 349, 474, 962, 1041, 1123, 1141, 1206
FauI CCCGC 1 cut(s) 1129
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 1 cut(s) 1080
FokI GGATG 2 cut(s) 857, 1252
FriOI GRGCYC 1 cut(s) 1193
FspBI CTAG 3 cut(s) 203, 402, 923
GlaI GCGC 2 cut(s) 176, 544
GsuI CTGGAG 1 cut(s) 319
HaeII RGCGCY 1 cut(s) 546
HapII CCGG 9 cut(s) 78, 141, 186, 243, 316, 336, 519, 534, 1007
HgaI GACGC 2 cut(s) 687, 1050
HhaI GCGC 2 cut(s) 177, 545
Hin1II CATG 7 cut(s) 353, 478, 966, 1045, 1127, 1145, 1210
Hin6I GCGC 2 cut(s) 175, 543
HinP1I GCGC 2 cut(s) 175, 543
HinfI GANTC 4 cut(s) 112, 128, 165, 1274
HpaII CCGG 9 cut(s) 78, 141, 186, 243, 316, 336, 519, 534, 1007
HphI GGTGA 7 cut(s) 46, 61, 160, 184, 443, 694, 715
Hpy166II GTNNAC 5 cut(s) 217, 1081, 1252, 1308, 1408
Hpy188I TCNGA 6 cut(s) 263, 469, 526, 1087, 1245, 1489
Hpy8I GTNNAC 5 cut(s) 217, 1081, 1252, 1308, 1408
Hpy99I CGWCG 1 cut(s) 703
HpyAV CCTTC 2 cut(s) 872, 1025
HpyCH4III ACNGT 6 cut(s) 95, 283, 465, 627, 1078, 1325
HpyCH4IV ACGT 2 cut(s) 252, 1050
HpyCH4V TGCA 9 cut(s) 353, 551, 1045, 1252, 1308, 1392, 1429, 1443, 1478
HpyF10VI GCNNNNNNNGC 7 cut(s) 144, 186, 359, 554, 557, 563, 810
HpyF3I CTNAG 4 cut(s) 1084, 1154, 1242, 1434
HpySE526I ACGT 2 cut(s) 252, 1050
Hsp92II CATG 7 cut(s) 353, 478, 966, 1045, 1127, 1145, 1210
HspAI GCGC 2 cut(s) 175, 543
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 445
KspI CCGCGG 1 cut(s) 564
Kzo9I GATC 4 cut(s) 247, 363, 445, 1504
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 6 cut(s) 80, 143, 669, 1196, 1262, 1342
Lsp1109I GCAGC 4 cut(s) 331, 535, 746, 1070
LweI GCATC 4 cut(s) 88, 229, 279, 1274
MaeI CTAG 3 cut(s) 203, 402, 923
MaeII ACGT 2 cut(s) 252, 1050
MaeIII GTNAC 2 cut(s) 166, 283
MalI GATC 4 cut(s) 249, 365, 447, 1506
MboI GATC 4 cut(s) 247, 363, 445, 1504
MfeI CAATTG 1 cut(s) 1117
MhlI GDGCHC 4 cut(s) 962, 1193, 1254, 1310
MluCI AATT 8 cut(s) 88, 341, 646, 786, 1117, 1399, 1420, 1516
MlyI GAGTC 3 cut(s) 121, 174, 1283
MmeI TCCRAC 4 cut(s) 447, 504, 1218, 1357
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 3 cut(s) 714, 789, 849
MspA1I CMGCKG 2 cut(s) 548, 563
MspI CCGG 9 cut(s) 78, 141, 186, 243, 316, 336, 519, 534, 1007
MspR9I CCNGG 6 cut(s) 172, 298, 519, 520, 1313, 1336
MunI CAATTG 1 cut(s) 1117
MvaI CCWGG 4 cut(s) 172, 298, 1313, 1336
MvnI CGCG 2 cut(s) 177, 563
MwoI GCNNNNNNNGC 7 cut(s) 144, 186, 359, 554, 557, 563, 810
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 2 cut(s) 519, 520
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 4 cut(s) 247, 363, 445, 1504
NgoMIV GCCGGC 1 cut(s) 242
NlaIII CATG 7 cut(s) 353, 478, 966, 1045, 1127, 1145, 1210
NlaIV GGNNCC 2 cut(s) 1192, 1344
NmuCI GTSAC 2 cut(s) 166, 283
NotI GCGGCCGC 2 cut(s) 330, 558
NspI RCATGY 2 cut(s) 353, 1045
NspV TTCGAA 1 cut(s) 126
PasI CCCWGGG 1 cut(s) 1312
PceI AGGCCT 1 cut(s) 1014
PciSI GCTCTTC 1 cut(s) 680
PdiI GCCGGC 1 cut(s) 244
PfeI GAWTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 296
PleI GAGTC 3 cut(s) 120, 173, 1282
PpsI GAGTC 3 cut(s) 120, 173, 1282
PsiI TTATAA 1 cut(s) 1449
Psp6I CCWGG 4 cut(s) 170, 296, 1311, 1334
PspGI CCWGG 4 cut(s) 170, 296, 1311, 1334
PspN4I GGNNCC 2 cut(s) 1192, 1344
PspPI GGNCC 4 cut(s) 526, 1138, 1229, 1382
PsrI GAACNNNNNNTAC 2 cut(s) 1196, 1228
RsaI GTAC 5 cut(s) 155, 749, 1022, 1049, 1331
RsaNI GTAC 5 cut(s) 154, 748, 1021, 1048, 1330
SacII CCGCGG 1 cut(s) 564
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 714, 789, 849
Sau3AI GATC 4 cut(s) 247, 363, 445, 1504
Sau96I GGNCC 4 cut(s) 526, 1138, 1229, 1382
SchI GAGTC 3 cut(s) 121, 174, 1283
ScrFI CCNGG 6 cut(s) 172, 298, 519, 520, 1313, 1336
SduI GDGCHC 4 cut(s) 962, 1193, 1254, 1310
SfaNI GCATC 4 cut(s) 88, 229, 279, 1274
SfiI GGCCNNNNNGGCC 1 cut(s) 186
Sfr303I CCGCGG 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 126
SgrBI CCGCGG 1 cut(s) 564
SinI GGWCC 2 cut(s) 526, 1229
SmaI CCCGGG 1 cut(s) 520
SpeI ACTAGT 1 cut(s) 401
Sse9I AATT 8 cut(s) 88, 341, 646, 786, 1117, 1399, 1420, 1516
SseBI AGGCCT 1 cut(s) 1014
SspI AATATT 1 cut(s) 773
SspMI CTAG 3 cut(s) 203, 402, 923
StuI AGGCCT 1 cut(s) 1014
StyD4I CCNGG 6 cut(s) 170, 296, 517, 518, 1311, 1334
StyI CCWWGG 2 cut(s) 567, 1123
TaaI ACNGT 6 cut(s) 95, 283, 465, 627, 1078, 1325
TaiI ACGT 2 cut(s) 255, 1053
TaqI TCGA 2 cut(s) 126, 1103
TasI AATT 8 cut(s) 88, 341, 646, 786, 1117, 1399, 1420, 1516
TatI WGTACW 2 cut(s) 747, 1020
TauI GCSGC 9 cut(s) 147, 180, 332, 335, 548, 560, 563, 566, 986
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 3 cut(s) 714, 789, 849
Tru9I TTAA 3 cut(s) 714, 789, 849
TscAI CASTG 3 cut(s) 100, 288, 448
TseFI GTSAC 2 cut(s) 166, 283
TseI GCWGC 4 cut(s) 319, 548, 734, 1058
Tsp45I GTSAC 2 cut(s) 166, 283
TspDTI ATGAA 4 cut(s) 376, 1158, 1288, 1524
TspGWI ACGGA 2 cut(s) 1122, 1258
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 3 cut(s) 100, 288, 448
VneI GTGCAC 2 cut(s) 1250, 1306
VpaK11BI GGWCC 2 cut(s) 526, 1229
XapI RAATTY 1 cut(s) 1516
XceI RCATGY 2 cut(s) 353, 1045
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 1080
XspI CTAG 3 cut(s) 203, 402, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.