Rh5BG281100

Endoglucanase 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004610
Physical Location & Seq
Reverse (-)
35487240 .. 35489988
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG281100.1

Sequence Viewer

Length: 1272 bp
ATGGCGTTTACTATAACGATGCTTTCTTGGGGTGTCATTGAATACGGCAAAGAAATAACGGATTCCGGCGAGTACAGCCATGCCCTTGAATCCATTAAATGGGGGACAGACTATTTCATAAAAGCTCATACTCATCCAAATGTTTTGTGGGCGGAGGTGGGTGATGGATTCACGGATCACTACTGTTGGCAAAGACCAGAAGACATGACAACGTCTCGTCAATCCTACAAAGTCGATGAAAAAAACCCCGGATCAGATGTTGCCGGGGAGACAGCCGCGGCAATGGCAGCAGCTGCCATTGTGTTTAGGAAAACAAACCCACATTACTCAGGCCTCCTTTTAGACCATGCAAAACAGTTGTTTGAGTTTGGTGACAAATATAGAGGGAAGTATGATGAGAGCATTAAGGTGGTTAAAGGCCACTATACATCTTGGAGTGGATACATGGATGAGTTGCTCTGGGCAGCTATGTGGTTGTACAAGGCTACAAACAATGAAGATTATTTGAACTATGTTTTGAACAATGCTCAAGCTTTTGGAGGCACCACTTGGTCCATCACAGAGTTTAGCTGGGATGTTAAGTATGCTGGTGTTCAAATCATTGCTTCAATGTTGCTTAAGGAAGCAAAGCACAAGCAGCACGAGCACATACTGAAACAATACCTATCAAAAGCAGAATACTACATGTGTGCTTGCCTTGGCAAGAACAACAAGACCAACGTGCAACGGACCCCAGGAGGCCTACTGTACATCCGCCAATGGAACAACATGCAATACGTTTCAAATGCTGCATTTCTCCTCACAGTTTACTTTGATCATCTCCAGGCCACTCATCAGAATCTAAACTGCGACAAAGGTCAGGTTGGCCCTGAAGAAATGTTAGCTTTTGCCAAGTCACAGGTTGATTACATTTTGGGGTCTAATCCAATGGCCATGAGCTACTTGGTCGGGTACGGTCAAAACTACCCTCTGAGGCTGCACCACAGAGGAGCATCCATTGATTCGTATAAGAGGAACAAGGGATTTATTGGGTGCACGCAGGGGTATGATAACTGGTATGGGAGGAAGGATCCGAACCCAAATGTGGTTGTCGGAGCCTTAGTCGGTGGCCCGAACAGGGATGATGGATTCCGGGATGAGAGGTGGAATTACATGCAGACGGAGGCATGTACTTACAATACCGCAACATTGGTTGGGGTTTTTGCCAAGTTGCATAGATTAGAAGGTGATGGTGATCTGTCTCTTCATCAATCTCTATTAGCTTCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

423

Amino Acids

47.98

Weight (kDa)

6.35

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 400 1.3e-135 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 99
AccII CGCG 1 cut(s) 278
AciI CCGC 5 cut(s) 152, 276, 278, 754, 1182
AclWI GGATC 4 cut(s) 183, 259, 1064, 1077
AcoI YGGCCR 1 cut(s) 930
AcuI CTGAAG 1 cut(s) 891
AfaI GTAC 5 cut(s) 74, 479, 749, 953, 1171
AfiI CCNNNNNNNGG 3 cut(s) 99, 1084, 1117
AflII CTTAAG 1 cut(s) 617
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 7 cut(s) 41, 89, 508, 520, 596, 609, 783
AjnI CCWGG 2 cut(s) 733, 822
AluBI AGCT 9 cut(s) 125, 293, 467, 533, 570, 884, 939, 1262, 1269
AluI AGCT 9 cut(s) 125, 293, 467, 533, 570, 884, 939, 1262, 1269
Alw21I GWGCWC 2 cut(s) 648, 1037
Alw26I GTCTC 3 cut(s) 219, 263, 1245
Alw44I GTGCAC 1 cut(s) 1033
AlwI GGATC 4 cut(s) 183, 259, 1064, 1077
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 7 cut(s) 331, 418, 739, 825, 865, 930, 1108
ApaLI GTGCAC 1 cut(s) 1033
ApeKI GCWGC 7 cut(s) 287, 290, 293, 464, 637, 788, 976
AspS9I GGNCC 4 cut(s) 552, 729, 866, 1109
AsuC2I CCSGG 3 cut(s) 249, 265, 1133
AsuHPI GGTGA 4 cut(s) 173, 383, 1238, 1244
AvaII GGWCC 2 cut(s) 552, 729
BaeGI GKGCMC 1 cut(s) 1037
BalI TGGCCA 1 cut(s) 932
BamHI GGATCC 1 cut(s) 1069
BanI GGYRCC 1 cut(s) 542
BauI CACGAG 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 2 cut(s) 648, 1037
BbvI GCAGC 7 cut(s) 280, 299, 302, 476, 649, 775, 963
BccI CCATC 4 cut(s) 158, 563, 1118, 1223
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 735, 824
BciVI GTATCC 1 cut(s) 434
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 3 cut(s) 249, 265, 1133
BcoDI GTCTC 3 cut(s) 219, 263, 1245
BfaI CTAG 2 cut(s) 1266, 1270
BfrI CTTAAG 1 cut(s) 617
BfuI GTATCC 1 cut(s) 434
BisI GCNGC 9 cut(s) 276, 279, 288, 291, 294, 465, 638, 789, 977
BlsI GCNGC 9 cut(s) 277, 280, 289, 292, 295, 466, 639, 790, 978
Bme1390I CCNGG 5 cut(s) 249, 265, 735, 824, 1133
Bme18I GGWCC 2 cut(s) 552, 729
BmgT120I GGNCC 4 cut(s) 552, 729, 866, 1109
BmiI GGNNCC 4 cut(s) 544, 731, 1071, 1096
BmrFI CCNGG 5 cut(s) 249, 265, 735, 824, 1133
BmsI GCATC 2 cut(s) 9, 1001
BoxI GACNNNNGTC 1 cut(s) 855
BpiI GAAGAC 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 806
BpuEI CTTGAG 1 cut(s) 513
BpuMI CCSGG 3 cut(s) 249, 265, 1133
BsaBI GATNNNNATC 1 cut(s) 1233
BsaJI CCNNGG 5 cut(s) 247, 264, 276, 697, 733
BsaXI ACNNNNNCTCC 2 cut(s) 1086, 1116
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 1084, 1117
Bse1I ACTGG 1 cut(s) 1058
Bse3DI GCAATG 2 cut(s) 288, 600
Bse8I GATNNNNATC 1 cut(s) 1233
BseBI CCWGG 2 cut(s) 735, 824
BseDI CCNNGG 5 cut(s) 247, 264, 276, 697, 733
BseGI GGATG 7 cut(s) 133, 454, 580, 750, 992, 1126, 1141
BseJI GATNNNNATC 1 cut(s) 1233
BseLI CCNNNNNNNGG 3 cut(s) 99, 1084, 1117
BseMI GCAATG 2 cut(s) 288, 600
BseMII CTCAG 2 cut(s) 342, 962
BseNI ACTGG 1 cut(s) 1058
BseRI GAGGAG 2 cut(s) 788, 1002
BseSI GKGCMC 1 cut(s) 1037
BseXI GCAGC 7 cut(s) 280, 299, 302, 476, 649, 775, 963
BseYI CCCAGC 1 cut(s) 570
BsgI GTGCAG 1 cut(s) 962
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 7 cut(s) 333, 420, 741, 827, 867, 932, 1110
BshNI GGYRCC 1 cut(s) 542
BsiHKAI GWGCWC 2 cut(s) 648, 1037
BsiSI CCGG 4 cut(s) 66, 249, 264, 1132
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 3 cut(s) 99, 1084, 1117
BsmAI GTCTC 3 cut(s) 219, 263, 1245
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 7 cut(s) 333, 420, 741, 827, 867, 932, 1110
Bsp1286I GDGCHC 2 cut(s) 648, 1037
Bsp1407I TGTACA 2 cut(s) 477, 747
Bsp143I GATC 5 cut(s) 175, 251, 814, 1069, 1234
BspACI CCGC 5 cut(s) 152, 276, 278, 754, 1182
BspANI GGCC 7 cut(s) 333, 420, 741, 827, 867, 932, 1110
BspCNI CTCAG 2 cut(s) 341, 963
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 4 cut(s) 544, 731, 1071, 1096
BspPI GGATC 4 cut(s) 183, 259, 1064, 1077
BspT107I GGYRCC 1 cut(s) 542
BspTI CTTAAG 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 288, 600
BsrGI TGTACA 2 cut(s) 477, 747
BsrI ACTGG 1 cut(s) 1058
BssECI CCNNGG 5 cut(s) 247, 264, 276, 697, 733
BssMI GATC 5 cut(s) 175, 251, 814, 1069, 1234
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 697
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 2 cut(s) 735, 824
Bst4CI ACNGT 5 cut(s) 185, 357, 747, 805, 956
Bst6I CTCTTC 1 cut(s) 1248
BstAFI CTTAAG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 293
BstAUI TGTACA 2 cut(s) 477, 747
BstC8I GCNNGC 2 cut(s) 694, 1037
BstDEI CTNAG 3 cut(s) 328, 971, 1099
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 7 cut(s) 133, 454, 580, 750, 992, 1126, 1141
BstFNI CGCG 1 cut(s) 278
BstKTI GATC 5 cut(s) 178, 254, 817, 1072, 1237
BstMAI GTCTC 3 cut(s) 219, 263, 1245
BstMBI GATC 5 cut(s) 175, 251, 814, 1069, 1234
BstMWI GCNNNNNNNGC 6 cut(s) 75, 284, 287, 293, 637, 643
BstNI CCWGG 2 cut(s) 735, 824
BstNSI RCATGY 4 cut(s) 688, 772, 1156, 1170
BstPAI GACNNNNGTC 1 cut(s) 855
BstSCI CCNGG 5 cut(s) 247, 263, 733, 822, 1131
BstSLI GKGCMC 1 cut(s) 1037
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 7 cut(s) 280, 299, 302, 476, 649, 775, 963
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 7 cut(s) 333, 420, 741, 827, 867, 932, 1110
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 7 cut(s) 133, 454, 580, 750, 992, 1126, 1141
Cac8I GCNNGC 2 cut(s) 694, 1037
CaiI CAGNNNCTG 1 cut(s) 293
Cfr13I GGNCC 4 cut(s) 552, 729, 866, 1109
Cfr42I CCGCGG 1 cut(s) 279
Csp6I GTAC 5 cut(s) 73, 478, 748, 952, 1170
CviAII CATG 9 cut(s) 80, 205, 347, 445, 685, 769, 934, 1153, 1167
CviQI GTAC 5 cut(s) 73, 478, 748, 952, 1170
DdeI CTNAG 3 cut(s) 328, 971, 1099
DpnI GATC 5 cut(s) 177, 253, 816, 1071, 1236
DpnII GATC 5 cut(s) 175, 251, 814, 1069, 1234
EaeI YGGCCR 1 cut(s) 930
Eam1104I CTCTTC 1 cut(s) 1248
EarI CTCTTC 1 cut(s) 1248
EciI GGCGGA 2 cut(s) 167, 743
Eco130I CCWWGG 1 cut(s) 697
Eco147I AGGCCT 2 cut(s) 333, 741
Eco47I GGWCC 2 cut(s) 552, 729
Eco57I CTGAAG 1 cut(s) 891
EcoRII CCWGG 2 cut(s) 733, 822
EcoT14I CCWWGG 1 cut(s) 697
ErhI CCWWGG 1 cut(s) 697
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 9 cut(s) 83, 208, 350, 448, 688, 772, 937, 1156, 1170
FaqI GGGAC 1 cut(s) 118
FatI CATG 9 cut(s) 79, 204, 346, 444, 684, 768, 933, 1152, 1166
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 9 cut(s) 276, 279, 288, 291, 294, 465, 638, 789, 977
FokI GGATG 7 cut(s) 120, 461, 587, 737, 979, 1133, 1148
Fsp4HI GCNGC 9 cut(s) 276, 279, 288, 291, 294, 465, 638, 789, 977
FspBI CTAG 2 cut(s) 1266, 1270
GluI GCNGC 9 cut(s) 276, 279, 288, 291, 294, 465, 638, 789, 977
GsaI CCCAGC 1 cut(s) 574
GsuI CTGGAG 1 cut(s) 806
HaeIII GGCC 7 cut(s) 333, 420, 741, 827, 867, 932, 1110
HapII CCGG 4 cut(s) 66, 249, 264, 1132
Hin1II CATG 9 cut(s) 83, 208, 350, 448, 688, 772, 937, 1156, 1170
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 6 cut(s) 62, 89, 168, 838, 1001, 1128
HpaII CCGG 4 cut(s) 66, 249, 264, 1132
HphI GGTGA 4 cut(s) 173, 383, 1238, 1244
Hpy166II GTNNAC 3 cut(s) 9, 808, 1035
Hpy188I TCNGA 5 cut(s) 256, 837, 972, 1074, 1094
Hpy8I GTNNAC 3 cut(s) 9, 808, 1035
HpyAV CCTTC 2 cut(s) 1060, 1217
HpyCH4III ACNGT 5 cut(s) 185, 357, 747, 805, 956
HpyCH4IV ACGT 3 cut(s) 212, 720, 777
HpyCH4V TGCA 8 cut(s) 350, 724, 772, 791, 979, 1035, 1156, 1213
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 284, 287, 293, 637, 643
HpyF3I CTNAG 3 cut(s) 328, 971, 1099
HpySE526I ACGT 3 cut(s) 212, 720, 777
Hsp92II CATG 9 cut(s) 83, 208, 350, 448, 688, 772, 937, 1156, 1170
Ksp22I TGATCA 1 cut(s) 814
KspI CCGCGG 1 cut(s) 279
Kzo9I GATC 5 cut(s) 175, 251, 814, 1069, 1234
LmnI GCTCC 2 cut(s) 989, 1094
Lsp1109I GCAGC 7 cut(s) 280, 299, 302, 476, 649, 775, 963
LweI GCATC 2 cut(s) 9, 1001
MaeI CTAG 2 cut(s) 1266, 1270
MaeII ACGT 3 cut(s) 212, 720, 777
MaeIII GTNAC 2 cut(s) 371, 894
MalI GATC 5 cut(s) 177, 253, 816, 1071, 1236
MboI GATC 5 cut(s) 175, 251, 814, 1069, 1234
MboII GAAGA 4 cut(s) 212, 509, 884, 1235
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 2 cut(s) 648, 1037
MlsI TGGCCA 1 cut(s) 932
MluCI AATT 1 cut(s) 1147
MluNI TGGCCA 1 cut(s) 932
MmeI TCCRAC 1 cut(s) 1072
Mox20I TGGCCA 1 cut(s) 932
MscI TGGCCA 1 cut(s) 932
MseI TTAA 5 cut(s) 96, 405, 414, 579, 618
MslI CAYNNNNRTG 2 cut(s) 138, 407
Msp20I TGGCCA 1 cut(s) 932
MspA1I CMGCKG 2 cut(s) 278, 293
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 4 cut(s) 66, 249, 264, 1132
MspR9I CCNGG 5 cut(s) 249, 265, 735, 824, 1133
MvaI CCWGG 2 cut(s) 735, 824
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 6 cut(s) 75, 284, 287, 293, 637, 643
NciI CCSGG 3 cut(s) 249, 265, 1133
NdeII GATC 5 cut(s) 175, 251, 814, 1069, 1234
NlaIII CATG 9 cut(s) 83, 208, 350, 448, 688, 772, 937, 1156, 1170
NlaIV GGNNCC 4 cut(s) 544, 731, 1071, 1096
NmuCI GTSAC 2 cut(s) 371, 894
NspI RCATGY 4 cut(s) 688, 772, 1156, 1170
PceI AGGCCT 2 cut(s) 333, 741
PciI ACATGT 1 cut(s) 684
PfeI GAWTC 6 cut(s) 62, 89, 168, 838, 1001, 1128
PflFI GACNNNGTC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 99
PfoI TCCNGGA 1 cut(s) 1131
PkrI GCNGC 9 cut(s) 277, 280, 289, 292, 295, 466, 639, 790, 978
PscI ACATGT 1 cut(s) 684
PshAI GACNNNNGTC 1 cut(s) 855
Psp6I CCWGG 2 cut(s) 733, 822
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 2 cut(s) 733, 822
PspN4I GGNNCC 4 cut(s) 544, 731, 1071, 1096
PspPI GGNCC 4 cut(s) 552, 729, 866, 1109
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 1069
PsyI GACNNNGTC 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 5 cut(s) 74, 479, 749, 953, 1171
RsaNI GTAC 5 cut(s) 73, 478, 748, 952, 1170
RseI CAYNNNNRTG 2 cut(s) 138, 407
SacII CCGCGG 1 cut(s) 279
SaqAI TTAA 5 cut(s) 96, 405, 414, 579, 618
SatI GCNGC 9 cut(s) 276, 279, 288, 291, 294, 465, 638, 789, 977
Sau3AI GATC 5 cut(s) 175, 251, 814, 1069, 1234
Sau96I GGNCC 4 cut(s) 552, 729, 866, 1109
ScrFI CCNGG 5 cut(s) 249, 265, 735, 824, 1133
SduI GDGCHC 2 cut(s) 648, 1037
SfaNI GCATC 2 cut(s) 9, 1001
Sfr303I CCGCGG 1 cut(s) 279
SgrBI CCGCGG 1 cut(s) 279
SinI GGWCC 2 cut(s) 552, 729
SmiMI CAYNNNNRTG 2 cut(s) 138, 407
SmlI CTYRAG 2 cut(s) 528, 617
SmoI CTYRAG 2 cut(s) 528, 617
Sse9I AATT 1 cut(s) 1147
SseBI AGGCCT 2 cut(s) 333, 741
SsiI CCGC 5 cut(s) 152, 276, 278, 754, 1182
SspMI CTAG 2 cut(s) 1266, 1270
StuI AGGCCT 2 cut(s) 333, 741
StyD4I CCNGG 5 cut(s) 247, 263, 733, 822, 1131
StyI CCWWGG 1 cut(s) 697
TaaI ACNGT 5 cut(s) 185, 357, 747, 805, 956
TaiI ACGT 3 cut(s) 215, 723, 780
TaqI TCGA 1 cut(s) 234
TasI AATT 1 cut(s) 1147
TatI WGTACW 4 cut(s) 72, 477, 747, 1169
TauI GCSGC 2 cut(s) 278, 281
TfiI GAWTC 6 cut(s) 62, 89, 168, 838, 1001, 1128
Tru1I TTAA 5 cut(s) 96, 405, 414, 579, 618
Tru9I TTAA 5 cut(s) 96, 405, 414, 579, 618
TseFI GTSAC 2 cut(s) 371, 894
TseI GCWGC 7 cut(s) 287, 290, 293, 464, 637, 788, 976
Tsp45I GTSAC 2 cut(s) 371, 894
TspDTI ATGAA 4 cut(s) 106, 252, 510, 1235
TspGWI ACGGA 4 cut(s) 74, 188, 742, 1175
Tth111I GACNNNGTC 1 cut(s) 211
Van91I CCANNNNNTGG 1 cut(s) 99
Vha464I CTTAAG 1 cut(s) 617
VneI GTGCAC 1 cut(s) 1033
VpaK11BI GGWCC 2 cut(s) 552, 729
XceI RCATGY 4 cut(s) 688, 772, 1156, 1170
XcmI CCANNNNNNNNNTGG 2 cut(s) 144, 940
XspI CTAG 2 cut(s) 1266, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.