MD03G1194800.v1.1

Endoglucanase 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004610
Physical Location & Seq
Forward (+)
26686280 .. 26688758
2479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1194800.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGAAGCAAGGTATTGTCCTCCAATGGTTTTCTCTACTCTCCATTTTCACACTCTTTTCCTTGTCTCAGTCCTTCGACTACGGCGAAGCTCTCTCCAAGAGTCTCCTCTACTTTGAGTCCCAACGTTCCGGCCGCTTGCCCCACAACCAACGGGTCACCTGGCGTCACCATTCCGGCCTCAACGATGGCCTAGAACAAGGGGTAGACTTGGTGGGAGGGTACTATGATGCTGGTGATAATGTGAAATTTGGTCTGCCAATGGCTTTTACAATAACAATGCTCTCTTGGGGTGTAATTGAGTACGGCAAACAAATGGAGGATGCTGGAGAGTACAATCATGCCTTAGCAGCTATCAAATGGGGGACTGACTATTTCATCAAGGCTCATACTCACCCAAATGTTTTGTGGGCTGAGGTTGGCGATGGATCTACTGATCACTACTGTTGGCAAAGGCCGGAGGACATGACGACCTCTCGACAAGCCTACAAGGTTGATGGGAATCATCCCGGATCAGATATTGCCGGTGAGACTGCGGCCGCTACGGCAGCGGCTGCCATTGTGTTTAGGAAAACCAACCCACATTACTCTGGCCTACTTCTACACCATGCACAACAGTTGTTTGAATTTGGTGACAAATATAGAGGGAAGTATGATGAGAGTATTGAAGTGGTGAAAGGGCACTACACATCTTGGAGTGGTTACATGGATGAGTTGCTTTGGGCAGCTTTGTGGTTGTACAAAGCCACAAACAATGAGGATTATTTGAACTATGTTTTGAACAATGCTCAGTCTCTTGGGGGCACTACTTGGGCCATCAAAGAGTTCAGCTGGGATGTTAAGTATGCTGGTGTTCAAATCATTGCTTCAATGTTTCTAAAGGAAGAGAAGCACAAGCAACACATGCACCTACTGCAACAGTACCGTTCTAAAGCAGAGTTTTACATGTGTGCTTGCCTTGACAAAAACAATGTAACCAACGTGCAACGCACCCCAGGAGGATTATTGTACATCCGCCAATGGAACAACATGCAATACGTTTCAAACGCAGCGTTTTTACTCACAATATACTCTGATTACATCCAAGCCGCTCATCACAATCTTACTTGCGACAAAGGACAGGTGGGCCCACAAGAAATCTTATCGTTTACCAAATCACAGGTTGATTACATTCTGGGGTCCAATCCAATGGGCATGAGCTACTTGGTTGGGTATGGACCAAAATACCCTCGAAGGCTGCACCACAGAGGAGCATCCATTGATTCGTATAAGAAAAACGAGGGTTTCATTGGGTGCACTCAGGGGTACCATAACTGGTATGGGAGGCAGGGTCCAAACCCTAACGTGCTTGTTGGGGCACTGGTTGGTGGGCCCGATGGCAACGATGGATTCAGAGATGAAAGGTGGAATTACATGCAGACTGAGGCATGCACTTATAACACTGCAACATTAGTGGGAGTTCTTGCTAAATTGGATGGATTAGAAGGTGATTATCTGTCTAATCAACCATTAATAGCTTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

508

Amino Acids

57.21

Weight (kDa)

6.2

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 27 - 487 5.4e-168 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1434
Acc65I GGTACC 1 cut(s) 1302
AccB1I GGYRCC 1 cut(s) 1302
AccBSI CCGCTC 1 cut(s) 1088
AccI GTMKAC 1 cut(s) 204
AciI CCGC 6 cut(s) 133, 533, 537, 548, 1012, 1086
AclI AACGTT 1 cut(s) 124
AclWI GGATC 2 cut(s) 433, 517
AcoI YGGCCR 2 cut(s) 130, 534
AcsI RAATTY 2 cut(s) 245, 623
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 7 cut(s) 221, 302, 332, 737, 920, 1007, 1304
AflIII ACRYGT 1 cut(s) 942
AgsI TTSAA 8 cut(s) 623, 665, 766, 778, 854, 867, 1041, 1518
AjnI CCWGG 2 cut(s) 158, 991
AluBI AGCT 7 cut(s) 89, 350, 725, 828, 1197, 1514, 1521
AluI AGCT 7 cut(s) 89, 350, 725, 828, 1197, 1514, 1521
Alw21I GWGCWC 1 cut(s) 1295
Alw26I GTCTC 4 cut(s) 69, 107, 521, 795
Alw44I GTGCAC 1 cut(s) 1291
AlwI GGATC 2 cut(s) 433, 517
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 9 cut(s) 130, 175, 187, 452, 534, 589, 810, 1123, 1367
ApaI GGGCCC 2 cut(s) 1127, 1371
ApaLI GTGCAC 1 cut(s) 1291
ApeKI GCWGC 6 cut(s) 347, 545, 551, 722, 1046, 1234
ApoI RAATTY 2 cut(s) 245, 623
AseI ATTAAT 1 cut(s) 1508
Asp718I GGTACC 1 cut(s) 1302
AspS9I GGNCC 8 cut(s) 810, 1123, 1124, 1176, 1214, 1328, 1367, 1368
AsuC2I CCSGG 1 cut(s) 507
AsuHPI GGTGA 8 cut(s) 148, 158, 245, 383, 536, 641, 682, 1496
AvaII GGWCC 3 cut(s) 1176, 1214, 1328
BaeGI GKGCMC 6 cut(s) 681, 803, 1127, 1295, 1357, 1371
BanI GGYRCC 1 cut(s) 1302
BanII GRGCYC 2 cut(s) 1127, 1371
Bbv12I GWGCWC 1 cut(s) 1295
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 6 cut(s) 359, 538, 557, 734, 1058, 1221
BccI CCATC 7 cut(s) 179, 416, 488, 821, 1367, 1376, 1466
BceAI ACGGC 3 cut(s) 97, 319, 558
BciT130I CCWGG 2 cut(s) 160, 993
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 507
BcoDI GTCTC 4 cut(s) 69, 107, 521, 795
BfaI CTAG 2 cut(s) 191, 1522
BglI GCCNNNNNGGC 1 cut(s) 542
Bme1390I CCNGG 3 cut(s) 160, 507, 993
Bme18I GGWCC 3 cut(s) 1176, 1214, 1328
BmgT120I GGNCC 8 cut(s) 810, 1123, 1124, 1176, 1214, 1328, 1367, 1368
BmiI GGNNCC 5 cut(s) 1125, 1177, 1304, 1329, 1369
BmrFI CCNGG 3 cut(s) 160, 507, 993
BmsI GCATC 3 cut(s) 217, 310, 1259
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 2 cut(s) 343, 411
BpuMI CCSGG 1 cut(s) 507
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 991
BsaXI ACNNNNNCTCC 2 cut(s) 1312, 1342
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 2 cut(s) 1316, 1362
Bse3DI GCAATG 1 cut(s) 858
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 2 cut(s) 160, 993
BseDI CCNNGG 1 cut(s) 991
BseGI GGATG 8 cut(s) 325, 502, 712, 838, 1008, 1077, 1250, 1477
BseJI GATNNNNATC 1 cut(s) 498
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 5 cut(s) 80, 402, 800, 1310, 1410
BseNI ACTGG 2 cut(s) 1316, 1362
BseRI GAGGAG 2 cut(s) 95, 1260
BseSI GKGCMC 6 cut(s) 681, 803, 1127, 1295, 1357, 1371
BseX3I CGGCCG 2 cut(s) 130, 534
BseXI GCAGC 6 cut(s) 359, 538, 557, 734, 1058, 1221
BseYI CCCAGC 1 cut(s) 828
BsgI GTGCAG 1 cut(s) 1220
Bsh1285I CGRYCG 2 cut(s) 133, 537
BshFI GGCC 9 cut(s) 132, 177, 189, 454, 536, 591, 812, 1125, 1369
BshNI GGYRCC 1 cut(s) 1302
BsiEI CGRYCG 2 cut(s) 133, 537
BsiHKAI GWGCWC 1 cut(s) 1295
BsiSI CCGG 5 cut(s) 129, 174, 455, 507, 522
BslFI GGGAC 2 cut(s) 103, 376
BsmAI GTCTC 4 cut(s) 69, 107, 521, 795
BsmFI GGGAC 2 cut(s) 103, 376
BsnI GGCC 9 cut(s) 132, 177, 189, 454, 536, 591, 812, 1125, 1369
Bsp120I GGGCCC 2 cut(s) 1123, 1367
Bsp1286I GDGCHC 6 cut(s) 681, 803, 1127, 1295, 1357, 1371
Bsp1407I TGTACA 2 cut(s) 735, 1005
Bsp143I GATC 3 cut(s) 425, 433, 509
BspACI CCGC 6 cut(s) 133, 533, 537, 548, 1012, 1086
BspANI GGCC 9 cut(s) 132, 177, 189, 454, 536, 591, 812, 1125, 1369
BspCNI CTCAG 5 cut(s) 79, 403, 799, 1309, 1411
BspLI GGNNCC 5 cut(s) 1125, 1177, 1304, 1329, 1369
BspPI GGATC 2 cut(s) 433, 517
BspT107I GGYRCC 1 cut(s) 1302
BsrBI CCGCTC 1 cut(s) 1088
BsrDI GCAATG 1 cut(s) 858
BsrFI RCCGGY 1 cut(s) 521
BsrGI TGTACA 2 cut(s) 735, 1005
BsrI ACTGG 2 cut(s) 1316, 1362
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 1 cut(s) 991
BssMI GATC 3 cut(s) 425, 433, 509
BssNI GRCGYC 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 160, 993
Bst4CI ACNGT 4 cut(s) 443, 615, 918, 923
Bst6I CTCTTC 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 163
BstAPI GCANNNNNTGC 3 cut(s) 551, 901, 910
BstAUI TGTACA 2 cut(s) 735, 1005
BstC8I GCNNGC 3 cut(s) 137, 952, 1426
BstDEI CTNAG 6 cut(s) 66, 343, 411, 786, 1296, 1419
BstEII GGTNACC 1 cut(s) 154
BstF5I GGATG 8 cut(s) 325, 502, 712, 838, 1008, 1077, 1250, 1477
BstKTI GATC 3 cut(s) 428, 436, 512
BstMAI GTCTC 4 cut(s) 69, 107, 521, 795
BstMBI GATC 3 cut(s) 425, 433, 509
BstMCI CGRYCG 2 cut(s) 133, 537
BstMWI GCNNNNNNNGC 6 cut(s) 347, 542, 545, 551, 901, 910
BstNI CCWGG 2 cut(s) 160, 993
BstNSI RCATGY 5 cut(s) 904, 946, 1030, 1414, 1428
BstPI GGTNACC 1 cut(s) 154
BstSCI CCNGG 3 cut(s) 158, 505, 991
BstSLI GKGCMC 6 cut(s) 681, 803, 1127, 1295, 1357, 1371
BstV1I GCAGC 6 cut(s) 359, 538, 557, 734, 1058, 1221
BstX2I RGATCY 1 cut(s) 425
BstXI CCANNNNNNTGG 1 cut(s) 1186
BstYI RGATCY 1 cut(s) 425
BstZI CGGCCG 2 cut(s) 130, 534
BsuRI GGCC 9 cut(s) 132, 177, 189, 454, 536, 591, 812, 1125, 1369
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 8 cut(s) 325, 502, 712, 838, 1008, 1077, 1250, 1477
BtsI GCAGTG 1 cut(s) 1437
BtsIMutI CAGTG 2 cut(s) 1355, 1437
Cac8I GCNNGC 3 cut(s) 137, 952, 1426
CaiI CAGNNNCTG 1 cut(s) 551
CciNI GCGGCCGC 1 cut(s) 534
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 8 cut(s) 810, 1123, 1124, 1176, 1214, 1328, 1367, 1368
CseI GACGC 1 cut(s) 152
Csp6I GTAC 7 cut(s) 220, 301, 331, 736, 919, 1006, 1303
CspCI CAANNNNNGTGG 2 cut(s) 1431, 1466
CviQI GTAC 7 cut(s) 220, 301, 331, 736, 919, 1006, 1303
DdeI CTNAG 6 cut(s) 66, 343, 411, 786, 1296, 1419
DpnI GATC 3 cut(s) 427, 435, 511
DpnII GATC 3 cut(s) 425, 433, 509
EaeI YGGCCR 2 cut(s) 130, 534
EagI CGGCCG 2 cut(s) 130, 534
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
EciI GGCGGA 1 cut(s) 1001
EclXI CGGCCG 2 cut(s) 130, 534
Eco24I GRGCYC 2 cut(s) 1127, 1371
Eco47I GGWCC 3 cut(s) 1176, 1214, 1328
Eco52I CGGCCG 2 cut(s) 130, 534
Eco91I GGTNACC 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 158, 991
EcoT38I GRGCYC 2 cut(s) 1127, 1371
FaqI GGGAC 2 cut(s) 103, 376
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 1 cut(s) 204
FokI GGATG 8 cut(s) 332, 489, 719, 845, 995, 1064, 1237, 1484
FriOI GRGCYC 2 cut(s) 1127, 1371
FspBI CTAG 2 cut(s) 191, 1522
GsaI CCCAGC 1 cut(s) 832
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 9 cut(s) 132, 177, 189, 454, 536, 591, 812, 1125, 1369
HapII CCGG 5 cut(s) 129, 174, 455, 507, 522
HgaI GACGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 163
HinfI GANTC 5 cut(s) 100, 116, 499, 1259, 1386
HpaII CCGG 5 cut(s) 129, 174, 455, 507, 522
HphI GGTGA 8 cut(s) 148, 158, 245, 383, 536, 641, 682, 1496
Hpy166II GTNNAC 3 cut(s) 205, 1146, 1293
Hpy188I TCNGA 3 cut(s) 514, 1072, 1391
Hpy188III TCNNGA 1 cut(s) 474
Hpy8I GTNNAC 3 cut(s) 205, 1146, 1293
HpyAV CCTTC 3 cut(s) 82, 1224, 1475
HpyCH4III ACNGT 4 cut(s) 443, 615, 918, 923
HpyCH4IV ACGT 4 cut(s) 124, 978, 1035, 1341
HpyF10VI GCNNNNNNNGC 6 cut(s) 347, 542, 545, 551, 901, 910
HpyF3I CTNAG 6 cut(s) 66, 343, 411, 786, 1296, 1419
HpySE526I ACGT 4 cut(s) 124, 978, 1035, 1341
Hsp92I GRCGYC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 1306
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 3 cut(s) 425, 433, 509
LmnI GCTCC 1 cut(s) 1247
Lsp1109I GCAGC 6 cut(s) 359, 538, 557, 734, 1058, 1221
LweI GCATC 3 cut(s) 217, 310, 1259
MaeI CTAG 2 cut(s) 191, 1522
MaeII ACGT 4 cut(s) 124, 978, 1035, 1341
MaeIII GTNAC 5 cut(s) 154, 164, 629, 698, 970
MalI GATC 3 cut(s) 427, 435, 511
MbiI CCGCTC 1 cut(s) 1088
MboI GATC 3 cut(s) 425, 433, 509
MboII GAAGA 1 cut(s) 893
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 6 cut(s) 681, 803, 1127, 1295, 1357, 1371
MluCI AATT 5 cut(s) 245, 294, 623, 1405, 1466
MlyI GAGTC 2 cut(s) 109, 125
MseI TTAA 2 cut(s) 837, 1508
MslI CAYNNNNRTG 1 cut(s) 396
MspA1I CMGCKG 2 cut(s) 548, 828
MspI CCGG 5 cut(s) 129, 174, 455, 507, 522
MspR9I CCNGG 3 cut(s) 160, 507, 993
MvaI CCWGG 2 cut(s) 160, 993
MwoI GCNNNNNNNGC 6 cut(s) 347, 542, 545, 551, 901, 910
NciI CCSGG 1 cut(s) 507
NdeII GATC 3 cut(s) 425, 433, 509
NlaIV GGNNCC 5 cut(s) 1125, 1177, 1304, 1329, 1369
NmuCI GTSAC 3 cut(s) 154, 164, 629
NotI GCGGCCGC 1 cut(s) 534
NspI RCATGY 5 cut(s) 904, 946, 1030, 1414, 1428
PaeI GCATGC 1 cut(s) 1428
PciI ACATGT 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 3 cut(s) 499, 1259, 1386
PfoI TCCNGGA 1 cut(s) 505
PleI GAGTC 2 cut(s) 108, 124
PpsI GAGTC 2 cut(s) 108, 124
PscI ACATGT 1 cut(s) 942
PshBI ATTAAT 1 cut(s) 1508
PsiI TTATAA 1 cut(s) 1434
Psp1406I AACGTT 1 cut(s) 124
Psp6I CCWGG 2 cut(s) 158, 991
PspEI GGTNACC 1 cut(s) 154
PspFI CCCAGC 1 cut(s) 828
PspGI CCWGG 2 cut(s) 158, 991
PspN4I GGNNCC 5 cut(s) 1125, 1177, 1304, 1329, 1369
PspOMI GGGCCC 2 cut(s) 1123, 1367
PspPI GGNCC 8 cut(s) 810, 1123, 1124, 1176, 1214, 1328, 1367, 1368
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 828
RsaI GTAC 7 cut(s) 221, 302, 332, 737, 920, 1007, 1304
RsaNI GTAC 7 cut(s) 220, 301, 331, 736, 919, 1006, 1303
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 2 cut(s) 837, 1508
Sau3AI GATC 3 cut(s) 425, 433, 509
Sau96I GGNCC 8 cut(s) 810, 1123, 1124, 1176, 1214, 1328, 1367, 1368
SchI GAGTC 2 cut(s) 109, 125
ScrFI CCNGG 3 cut(s) 160, 507, 993
SduI GDGCHC 6 cut(s) 681, 803, 1127, 1295, 1357, 1371
SfaNI GCATC 3 cut(s) 217, 310, 1259
SinI GGWCC 3 cut(s) 1176, 1214, 1328
SmiMI CAYNNNNRTG 1 cut(s) 396
SphI GCATGC 1 cut(s) 1428
Sse9I AATT 5 cut(s) 245, 294, 623, 1405, 1466
SsiI CCGC 6 cut(s) 133, 533, 537, 548, 1012, 1086
SspMI CTAG 2 cut(s) 191, 1522
StyD4I CCNGG 3 cut(s) 158, 505, 991
TaaI ACNGT 4 cut(s) 443, 615, 918, 923
TaiI ACGT 4 cut(s) 127, 981, 1038, 1344
TaqI TCGA 3 cut(s) 75, 475, 1228
TasI AATT 5 cut(s) 245, 294, 623, 1405, 1466
TatI WGTACW 3 cut(s) 330, 735, 1005
TauI GCSGC 5 cut(s) 135, 536, 539, 551, 1088
TfiI GAWTC 3 cut(s) 499, 1259, 1386
Tru1I TTAA 2 cut(s) 837, 1508
Tru9I TTAA 2 cut(s) 837, 1508
TscAI CASTG 2 cut(s) 1362, 1444
TseFI GTSAC 3 cut(s) 154, 164, 629
TseI GCWGC 6 cut(s) 347, 545, 551, 722, 1046, 1234
Tsp45I GTSAC 3 cut(s) 154, 164, 629
TspDTI ATGAA 4 cut(s) 17, 364, 1273, 1410
TspRI CASTG 2 cut(s) 1362, 1444
VneI GTGCAC 1 cut(s) 1291
VpaK11BI GGWCC 3 cut(s) 1176, 1214, 1328
VspI ATTAAT 1 cut(s) 1508
XapI RAATTY 2 cut(s) 245, 623
XceI RCATGY 5 cut(s) 904, 946, 1030, 1414, 1428
XcmI CCANNNNNNNNNTGG 2 cut(s) 402, 1313
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 2 cut(s) 191, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.