Rw2G044160

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004610
Physical Location & Seq
Forward (+)
70839560 .. 70842982
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044160.1

Sequence Viewer

Length: 1554 bp
ATGGTGAAGAACAAGGCACAACATTTTGCACAATGGTGCTTTCTTTCTCTCATCTTCGCCATTTTCACGACCGCGAAAGCTCTGAATTACGGCGACGCCCTCGCCAAGAGTCTCCTCTACTTTGAATCCCAACGCTCCGGCCGGTTGCCGTACAACCAGAGAGTCACCTGGCGGGACCATTCCGGCCTCACCGACGGCCTAGAACAAGGGGTGGACTTGGTAGGAGGCTATTATGACGCCGGTGACCACGTCAAGTTCGGGCTGCCAATGGCGTTTACTGTGACAATGCTCTCCTGGAGTGTCATCGAATACCGGCAGCAAATCGCCGACGCCGGCGAGCTCGAGCACGCGATGGAGGCCATCAAATGGGGGACCGACTACTTCATCAAAGCTCACACTAGTCCAAATGTGTTATGGGCCGAGGTGGGTGATGGAGACACTGACCATTACTGCTGGCAAAGACCGGAGGACATGACGACATCCCGGCAGGCGTACAAGATCGACCAGAACAATCCCGGGTCGGATCTCGCCGGAGAGACCGCGGCGGCAATGGCTGCCGCGGCCTTGGTGTTCAAGAAAACAAACCCGCATTACGCCCACCTTCTCCTACACCACGCCCAACAATTGTTCGAGTTTGGGGACAAGTATAGGGGGAAGTATGATGAGGAGGGGAGGATGGGAGTGGTGAAGAGCTACTATGCGTCGGTGAGTGGTTTCAAGGACGAGTTGTTGTGGGCAGCTTTGTGGCTATACAAGGCCACCGACAATGAGGAGTATTTGAGGTATGTAATTAACGAGGCTGAAAACTTTGGAGGCATTGGTTGGGCAATAACAGAGTTCAGCTGGGACGTTAAGTACGCTGGTATTCAAGTCATGGCTTCAAAGTTGCTAAATGAAGAGAAGTCCAAGAAACACAGTTCTACACTGGAAAAATATAGATCGAAAGCCGAGTACTACATCTGTTCATGCCTGAACAAAAACATTGACACCAAAAATGTGGAAAGAACACCAGCAGGCCTATTGTACATAAGGCAATGGAACAACATGCAGTATGTTTCCACAGCTTCATTCCTTCTAACCATGTATTCAGATTTCCTTAAAAGCTCGAACCAAAAGCTCAGCTGCCATGGAGGGATAGTGGGTCACCAAGAAATCCTCAGCTTTGCAAAAACACAGGTTGATTACATTTTGGGGTCTAATCCATCGAACATGAGCTACTTGGTGGGGTATGGTCCAAACTACCCTCAACGGGTGCACCACCGAGGCGCCTCTGTGGTGTCTTATAGAGAGAACAAAGCGTTCATTGGGTGTACACAAGGATATGATAACTGGTATGGTAGAGTTGAGCCAAACCCTAATGTTTTGGTCGGGGCTTTGGTTGGAGGGCCTGATTCTCAAGACAATTTTGTGGATCAGAGGGACAATTATATGCAGACTGAGGCCTGTACATATAATACAGCACCATTGGTTGGGGTTTTTGCTAAATTTTCAGAATTAGAGGTCCAAAAGGAGTTGGATCATATTCAGGATTTGGGTTTAGTTGCTTCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

517

Amino Acids

58.46

Weight (kDa)

5.83

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 492 3.7e-168 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1265
AccB7I CCANNNNNTGG 2 cut(s) 366, 1469
AccII CGCG 4 cut(s) 74, 350, 542, 560
AciI CCGC 8 cut(s) 72, 172, 540, 542, 545, 558, 560, 587
AclWI GGATC 3 cut(s) 531, 1419, 1524
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 1484
AcyI GRCGYC 4 cut(s) 96, 237, 330, 1266
AfaI GTAC 7 cut(s) 152, 494, 857, 953, 1025, 1312, 1447
AfiI CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
AgsI TTSAA 5 cut(s) 125, 574, 718, 869, 882
AhlI ACTAGT 1 cut(s) 398
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 2 cut(s) 167, 293
AleI CACNNNNGTG 1 cut(s) 34
Alw21I GWGCWC 3 cut(s) 342, 348, 1257
Alw26I GTCTC 3 cut(s) 116, 429, 530
Alw44I GTGCAC 1 cut(s) 1253
AlwI GGATC 3 cut(s) 531, 1419, 1524
Ama87I CYCGRG 2 cut(s) 341, 515
ApaLI GTGCAC 1 cut(s) 1253
ApeKI GCWGC 5 cut(s) 262, 316, 554, 737, 1122
ApoI RAATTY 1 cut(s) 1484
AspLEI GCGC 1 cut(s) 1268
AspS9I GGNCC 6 cut(s) 175, 372, 417, 1232, 1385, 1501
AsuC2I CCSGG 3 cut(s) 484, 516, 517
AsuHPI GGTGA 8 cut(s) 16, 157, 181, 254, 440, 697, 718, 1136
AvaI CYCGRG 2 cut(s) 341, 515
AvaII GGWCC 4 cut(s) 175, 372, 1232, 1501
BaeGI GKGCMC 1 cut(s) 1257
BanI GGYRCC 1 cut(s) 1265
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 3 cut(s) 342, 348, 1257
BbvCI CCTCAGC 1 cut(s) 1157
BbvI GCAGC 5 cut(s) 249, 328, 541, 749, 1109
BccI CCATC 5 cut(s) 346, 368, 425, 670, 1210
BceAI ACGGC 3 cut(s) 106, 133, 211
BciT130I CCWGG 2 cut(s) 169, 295
BcnI CCSGG 3 cut(s) 484, 516, 517
BcoDI GTCTC 3 cut(s) 116, 429, 530
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 2 cut(s) 200, 399
BfoI RGCGCY 1 cut(s) 1269
BisI GCNGC 9 cut(s) 263, 317, 543, 546, 555, 558, 561, 738, 1123
BlpI GCTNAGC 1 cut(s) 1118
BlsI GCNGC 9 cut(s) 264, 318, 544, 547, 556, 559, 562, 739, 1124
BmcAI AGTACT 1 cut(s) 953
Bme1390I CCNGG 5 cut(s) 169, 295, 484, 516, 517
Bme18I GGWCC 4 cut(s) 175, 372, 1232, 1501
BmeT110I CYCGRG 2 cut(s) 341, 515
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 6 cut(s) 175, 372, 417, 1232, 1385, 1501
BmiI GGNNCC 3 cut(s) 176, 373, 1267
BmrFI CCNGG 5 cut(s) 169, 295, 484, 516, 517
BpmI CTGGAG 1 cut(s) 316
Bpu10I CCTNAGC 1 cut(s) 1157
Bpu1102I GCTNAGC 1 cut(s) 1118
BpuEI CTTGAG 1 cut(s) 1380
BpuMI CCSGG 3 cut(s) 484, 516, 517
BsaHI GRCGYC 4 cut(s) 96, 237, 330, 1266
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 7 cut(s) 420, 515, 540, 558, 564, 1126, 1261
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 2 cut(s) 1122, 1152
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
Bse118I RCCGGY 4 cut(s) 141, 239, 312, 332
Bse1I ACTGG 2 cut(s) 930, 1334
Bse3DI GCAATG 2 cut(s) 555, 1040
BseBI CCWGG 2 cut(s) 169, 295
BseDI CCNNGG 7 cut(s) 420, 515, 540, 558, 564, 1126, 1261
BseGI GGATG 2 cut(s) 479, 681
BseLI CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
BseMI GCAATG 2 cut(s) 555, 1040
BseMII CTCAG 3 cut(s) 1132, 1171, 1428
BseNI ACTGG 2 cut(s) 930, 1334
BseRI GAGGAG 3 cut(s) 104, 680, 785
BseSI GKGCMC 1 cut(s) 1257
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 5 cut(s) 249, 328, 541, 749, 1109
BseYI CCCAGC 1 cut(s) 843
Bsh1236I CGCG 4 cut(s) 74, 350, 542, 560
Bsh1285I CGRYCG 2 cut(s) 72, 142
BshNI GGYRCC 1 cut(s) 1265
BsiEI CGRYCG 2 cut(s) 72, 142
BsiHKAI GWGCWC 3 cut(s) 342, 348, 1257
BsiHKCI CYCGRG 2 cut(s) 341, 515
BslFI GGGAC 5 cut(s) 188, 385, 653, 860, 1433
BslI CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
BsmAI GTCTC 3 cut(s) 116, 429, 530
BsmFI GGGAC 5 cut(s) 188, 385, 653, 860, 1433
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 2 cut(s) 341, 515
Bsp1286I GDGCHC 3 cut(s) 342, 348, 1257
Bsp1407I TGTACA 3 cut(s) 1023, 1310, 1445
Bsp143I GATC 5 cut(s) 498, 523, 938, 1411, 1516
Bsp1720I GCTNAGC 1 cut(s) 1118
Bsp19I CCATGG 1 cut(s) 1126
BspACI CCGC 8 cut(s) 72, 172, 540, 542, 545, 558, 560, 587
BspCNI CTCAG 3 cut(s) 1131, 1170, 1429
BspFNI CGCG 4 cut(s) 74, 350, 542, 560
BspLI GGNNCC 3 cut(s) 176, 373, 1267
BspPI GGATC 3 cut(s) 531, 1419, 1524
BspQI GCTCTTC 1 cut(s) 683
BspT107I GGYRCC 1 cut(s) 1265
BspTNI GGTCTC 1 cut(s) 530
BsrDI GCAATG 2 cut(s) 555, 1040
BsrFI RCCGGY 4 cut(s) 141, 239, 312, 332
BsrGI TGTACA 3 cut(s) 1023, 1310, 1445
BsrI ACTGG 2 cut(s) 930, 1334
BssAI RCCGGY 4 cut(s) 141, 239, 312, 332
BssECI CCNNGG 7 cut(s) 420, 515, 540, 558, 564, 1126, 1261
BssMI GATC 5 cut(s) 498, 523, 938, 1411, 1516
BssNI GRCGYC 4 cut(s) 96, 237, 330, 1266
BssT1I CCWWGG 2 cut(s) 564, 1126
Bst2UI CCWGG 2 cut(s) 169, 295
Bst4CI ACNGT 2 cut(s) 280, 917
Bst6I CTCTTC 2 cut(s) 683, 891
BstACI GRCGYC 4 cut(s) 96, 237, 330, 1266
BstAPI GCANNNNNTGC 1 cut(s) 554
BstAUI TGTACA 3 cut(s) 1023, 1310, 1445
BstC8I GCNNGC 6 cut(s) 334, 338, 348, 455, 489, 1015
BstDEI CTNAG 3 cut(s) 1118, 1157, 1437
BstDSI CCRYGG 3 cut(s) 540, 558, 1126
BstEII GGTNACC 2 cut(s) 242, 1142
BstF5I GGATG 2 cut(s) 479, 681
BstFNI CGCG 4 cut(s) 74, 350, 542, 560
BstH2I RGCGCY 1 cut(s) 1269
BstHHI GCGC 1 cut(s) 1268
BstKTI GATC 5 cut(s) 501, 526, 941, 1414, 1519
BstMAI GTCTC 3 cut(s) 116, 429, 530
BstMBI GATC 5 cut(s) 498, 523, 938, 1411, 1516
BstMCI CGRYCG 2 cut(s) 72, 142
BstMWI GCNNNNNNNGC 4 cut(s) 356, 551, 554, 560
BstNI CCWGG 2 cut(s) 169, 295
BstNSI RCATGY 1 cut(s) 1048
BstPI GGTNACC 2 cut(s) 242, 1142
BstSCI CCNGG 5 cut(s) 167, 293, 482, 514, 515
BstSLI GKGCMC 1 cut(s) 1257
BstUI CGCG 4 cut(s) 74, 350, 542, 560
BstV1I GCAGC 5 cut(s) 249, 328, 541, 749, 1109
BstX2I RGATCY 1 cut(s) 523
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 1 cut(s) 523
BstZI CGGCCG 1 cut(s) 139
BtgI CCRYGG 3 cut(s) 540, 558, 1126
BtgZI GCGATG 1 cut(s) 365
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 2 cut(s) 479, 681
BtsIMutI CAGTG 2 cut(s) 438, 923
Cac8I GCNNGC 6 cut(s) 334, 338, 348, 455, 489, 1015
CfoI GCGC 1 cut(s) 1268
Cfr10I RCCGGY 4 cut(s) 141, 239, 312, 332
Cfr13I GGNCC 6 cut(s) 175, 372, 417, 1232, 1385, 1501
Cfr42I CCGCGG 2 cut(s) 543, 561
Cfr9I CCCGGG 1 cut(s) 515
CseI GACGC 4 cut(s) 104, 245, 338, 690
Csp6I GTAC 7 cut(s) 151, 493, 856, 952, 1024, 1311, 1446
CviAII CATG 7 cut(s) 472, 874, 966, 1045, 1081, 1127, 1210
CviQI GTAC 7 cut(s) 151, 493, 856, 952, 1024, 1311, 1446
DdeI CTNAG 3 cut(s) 1118, 1157, 1437
DinI GGCGCC 1 cut(s) 1267
DpnI GATC 5 cut(s) 500, 525, 940, 1413, 1518
DpnII GATC 5 cut(s) 498, 523, 938, 1411, 1516
EaeI YGGCCR 1 cut(s) 139
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 683, 891
EarI CTCTTC 2 cut(s) 683, 891
Ecl136II GAGCTC 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 564, 1126
Eco147I AGGCCT 2 cut(s) 1017, 1442
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 4 cut(s) 175, 372, 1232, 1501
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 2 cut(s) 341, 515
Eco91I GGTNACC 2 cut(s) 242, 1142
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 1385
EcoO65I GGTNACC 2 cut(s) 242, 1142
EcoRII CCWGG 2 cut(s) 167, 293
EcoT14I CCWWGG 2 cut(s) 564, 1126
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 1267
EheI GGCGCC 1 cut(s) 1267
ErhI CCWWGG 2 cut(s) 564, 1126
FaeI CATG 7 cut(s) 475, 877, 969, 1048, 1084, 1130, 1213
FaqI GGGAC 5 cut(s) 188, 385, 653, 860, 1433
FatI CATG 7 cut(s) 471, 873, 965, 1044, 1080, 1126, 1209
FauI CCCGC 2 cut(s) 165, 594
Fnu4HI GCNGC 9 cut(s) 263, 317, 543, 546, 555, 558, 561, 738, 1123
FokI GGATG 2 cut(s) 466, 688
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 9 cut(s) 263, 317, 543, 546, 555, 558, 561, 738, 1123
FspBI CTAG 2 cut(s) 200, 399
GlaI GCGC 1 cut(s) 1267
GluI GCNGC 9 cut(s) 263, 317, 543, 546, 555, 558, 561, 738, 1123
GsaI CCCAGC 1 cut(s) 847
GsuI CTGGAG 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 1269
HgaI GACGC 4 cut(s) 104, 245, 338, 690
HhaI GCGC 1 cut(s) 1268
Hin1I GRCGYC 4 cut(s) 96, 237, 330, 1266
Hin1II CATG 7 cut(s) 475, 877, 969, 1048, 1084, 1130, 1213
Hin6I GCGC 1 cut(s) 1266
HinP1I GCGC 1 cut(s) 1266
HinfI GANTC 4 cut(s) 109, 125, 162, 1391
HphI GGTGA 8 cut(s) 16, 157, 181, 254, 440, 697, 718, 1136
Hpy166II GTNNAC 5 cut(s) 214, 276, 1255, 1311, 1313
Hpy188I TCNGA 5 cut(s) 84, 523, 1090, 1416, 1492
Hpy188III TCNNGA 4 cut(s) 67, 574, 1397, 1526
Hpy8I GTNNAC 5 cut(s) 214, 276, 1255, 1311, 1313
Hpy99I CGWCG 4 cut(s) 98, 197, 332, 706
HpyAV CCTTC 2 cut(s) 611, 1082
HpyCH4III ACNGT 2 cut(s) 280, 917
HpyCH4IV ACGT 2 cut(s) 249, 849
HpyCH4V TGCA 5 cut(s) 29, 1048, 1166, 1255, 1432
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 551, 554, 560
HpyF3I CTNAG 3 cut(s) 1118, 1157, 1437
HpySE526I ACGT 2 cut(s) 249, 849
Hsp92I GRCGYC 4 cut(s) 96, 237, 330, 1266
Hsp92II CATG 7 cut(s) 475, 877, 969, 1048, 1084, 1130, 1213
HspAI GCGC 1 cut(s) 1266
KasI GGCGCC 1 cut(s) 1265
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
KspI CCGCGG 2 cut(s) 543, 561
Kzo9I GATC 5 cut(s) 498, 523, 938, 1411, 1516
LguI GCTCTTC 1 cut(s) 683
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 5 cut(s) 249, 328, 541, 749, 1109
MaeI CTAG 2 cut(s) 200, 399
MaeII ACGT 2 cut(s) 249, 849
MaeIII GTNAC 4 cut(s) 163, 242, 280, 1142
MalI GATC 5 cut(s) 500, 525, 940, 1413, 1518
MboI GATC 5 cut(s) 498, 523, 938, 1411, 1516
MboII GAAGA 4 cut(s) 19, 46, 700, 908
MfeI CAATTG 1 cut(s) 623
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 3 cut(s) 342, 348, 1257
MluCI AATT 7 cut(s) 85, 623, 789, 1402, 1423, 1484, 1493
Mly113I GGCGCC 1 cut(s) 1266
MlyI GAGTC 2 cut(s) 118, 171
MmeI TCCRAC 3 cut(s) 501, 1360, 1494
MreI CGCCGGCG 1 cut(s) 332
MroNI GCCGGC 1 cut(s) 332
MseI TTAA 3 cut(s) 792, 852, 1098
MslI CAYNNNNRTG 1 cut(s) 34
MspA1I CMGCKG 4 cut(s) 542, 560, 843, 1122
MspR9I CCNGG 5 cut(s) 169, 295, 484, 516, 517
MunI CAATTG 1 cut(s) 623
MvaI CCWGG 2 cut(s) 169, 295
MvnI CGCG 4 cut(s) 74, 350, 542, 560
MwoI GCNNNNNNNGC 4 cut(s) 356, 551, 554, 560
NaeI GCCGGC 1 cut(s) 334
NarI GGCGCC 1 cut(s) 1266
NciI CCSGG 3 cut(s) 484, 516, 517
NcoI CCATGG 1 cut(s) 1126
NdeII GATC 5 cut(s) 498, 523, 938, 1411, 1516
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 7 cut(s) 475, 877, 969, 1048, 1084, 1130, 1213
NlaIV GGNNCC 3 cut(s) 176, 373, 1267
NmeAIII GCCGAG 2 cut(s) 445, 973
NmuCI GTSAC 4 cut(s) 163, 242, 280, 1142
NspI RCATGY 1 cut(s) 1048
OliI CACNNNNGTG 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 341
PceI AGGCCT 2 cut(s) 1017, 1442
PciSI GCTCTTC 1 cut(s) 683
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 2 cut(s) 125, 1391
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 2 cut(s) 366, 1469
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 9 cut(s) 264, 318, 544, 547, 556, 559, 562, 739, 1124
PleI GAGTC 2 cut(s) 117, 170
PluTI GGCGCC 1 cut(s) 1269
PpsI GAGTC 2 cut(s) 117, 170
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 167, 293
PspEI GGTNACC 2 cut(s) 242, 1142
PspFI CCCAGC 1 cut(s) 843
PspGI CCWGG 2 cut(s) 167, 293
PspN4I GGNNCC 3 cut(s) 176, 373, 1267
PspPI GGNCC 6 cut(s) 175, 372, 417, 1232, 1385, 1501
PspXI VCTCGAGB 1 cut(s) 341
PsrI GAACNNNNNNTAC 2 cut(s) 1199, 1231
PsuI RGATCY 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 2 cut(s) 843, 1122
RsaI GTAC 7 cut(s) 152, 494, 857, 953, 1025, 1312, 1447
RsaNI GTAC 7 cut(s) 151, 493, 856, 952, 1024, 1311, 1446
RseI CAYNNNNRTG 1 cut(s) 34
SacI GAGCTC 1 cut(s) 342
SacII CCGCGG 2 cut(s) 543, 561
SapI GCTCTTC 1 cut(s) 683
SaqAI TTAA 3 cut(s) 792, 852, 1098
SatI GCNGC 9 cut(s) 263, 317, 543, 546, 555, 558, 561, 738, 1123
Sau3AI GATC 5 cut(s) 498, 523, 938, 1411, 1516
Sau96I GGNCC 6 cut(s) 175, 372, 417, 1232, 1385, 1501
ScaI AGTACT 1 cut(s) 953
SchI GAGTC 2 cut(s) 118, 171
ScrFI CCNGG 5 cut(s) 169, 295, 484, 516, 517
SduI GDGCHC 3 cut(s) 342, 348, 1257
SfoI GGCGCC 1 cut(s) 1267
Sfr274I CTCGAG 1 cut(s) 341
Sfr303I CCGCGG 2 cut(s) 543, 561
SgrAI CRCCGGYG 2 cut(s) 239, 332
SgrBI CCGCGG 2 cut(s) 543, 561
SinI GGWCC 4 cut(s) 175, 372, 1232, 1501
SlaI CTCGAG 1 cut(s) 341
SmaI CCCGGG 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 2 cut(s) 341, 1395
SmoI CTYRAG 2 cut(s) 341, 1395
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 7 cut(s) 85, 623, 789, 1402, 1423, 1484, 1493
SseBI AGGCCT 2 cut(s) 1017, 1442
SsiI CCGC 8 cut(s) 72, 172, 540, 542, 545, 558, 560, 587
SspDI GGCGCC 1 cut(s) 1265
SspMI CTAG 2 cut(s) 200, 399
SstI GAGCTC 1 cut(s) 342
StuI AGGCCT 2 cut(s) 1017, 1442
StyD4I CCNGG 5 cut(s) 167, 293, 482, 514, 515
StyI CCWWGG 2 cut(s) 564, 1126
TaaI ACNGT 2 cut(s) 280, 917
TaiI ACGT 2 cut(s) 252, 852
TaqI TCGA 7 cut(s) 306, 342, 501, 630, 941, 1106, 1205
TaqII GACCGA 1 cut(s) 389
TasI AATT 7 cut(s) 85, 623, 789, 1402, 1423, 1484, 1493
TatI WGTACW 4 cut(s) 951, 1023, 1310, 1445
TauI GCSGC 4 cut(s) 545, 548, 560, 563
TfiI GAWTC 2 cut(s) 125, 1391
Tru1I TTAA 3 cut(s) 792, 852, 1098
Tru9I TTAA 3 cut(s) 792, 852, 1098
TscAI CASTG 2 cut(s) 445, 930
TseFI GTSAC 4 cut(s) 163, 242, 280, 1142
TseI GCWGC 5 cut(s) 262, 316, 554, 737, 1122
Tsp45I GTSAC 4 cut(s) 163, 242, 280, 1142
TspDTI ATGAA 5 cut(s) 373, 909, 954, 1056, 1291
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 2 cut(s) 445, 930
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 2 cut(s) 366, 1469
VneI GTGCAC 1 cut(s) 1253
VpaK11BI GGWCC 4 cut(s) 175, 372, 1232, 1501
XapI RAATTY 1 cut(s) 1484
XceI RCATGY 1 cut(s) 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XhoI CTCGAG 1 cut(s) 341
XmaI CCCGGG 1 cut(s) 515
XspI CTAG 2 cut(s) 200, 399
ZrmI AGTACT 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.