Rroxscaffold_2G00091480

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004610
Physical Location & Seq
Reverse (-)
13018545 .. 13022412
3868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00091480.1

Sequence Viewer

Length: 1554 bp
ATGGTGAAGAACAAGGCACAACATTTTGCACAATGGTGCTTTCTTTCTCTCATCTTCGCCATTTTCACGACCGCGAAATCTTTGAATTACGGGGACGCCCTCGCCAAGAGTCTCCTCTACTTTGAATCCCAACGCTCCGGCCGGTTGCCGTACAACCAGAGAGTCACCTGGCGGGACCATTCCGGCCTCACCGACGGCCTAGAACAAGGGGTGGACTTGGTAGGAGGCTATTATGACGCCGGTGACCACGTCAAGTTCGGGCTGCCAATGGCGTTTACTGTGACAATGCTCTCCTGGAGTGTCATAGAATACCGGCAGCAAATCGCCGACGCCGGCGAGCTCGAGCACGCGATGGAGGCCATCAAATGGGGGACCGACTACTTCATCAAAGCTCACACTAGTCCAAATGTGTTATGGGCCGAGGTGGGTGATGGAGACACTGACCATTACTGCTGGCAAAGACCGGAGGACATGACGACATCCCGGCAGGCGTACAAGATAGACCAGAACAATCCCGGGTCGGATCTCGCCGGAGAGACCGCGGCGGCAATGGCGGCCGCAGCCTTAGTGTTCAAGAAAACAAACCCGCATTATGCCCACCTTCTCCTACACCACGCCCAACAATTGTTCGAGTTTGGGGACAACTATAGGGGGAAGTATGATGAGGAGGGGAGGATGGGAGTGGTGAAGAGCTACTATGCGTCGGTGAGTGGTTTCAAGGACGAGTTGTTGTGGGCAGCTCTGTGGTTATACAAGGCCACCGACAATGAGGAGTATTTGAGGTATGTAATTAAAGAGGCTGAAAACTTTGGAGGCATTGGTTGGGCAATAACAGAGTTCAGCTGGGACGTTAAGTACGCTGGTCTTCAAGTCATGGCTGCAAAGTTGCTGAATGAAGAGAAGCCCAAGAAACACAGTACTACACTGGAAAAATATAGATCGAAAGCCGAGTACTACATCTGTTCATGCCTGAACAAAAACATTGACACCAAAAATGTGGAAAGAACACCAGCAGGCCTATTGTACATAAGGCAATGGAACAACATGCAGTATGTTTCCACAGCTTCATTCCTTCTAACCATGTATTCGGATTTCCTCAAAAGCTCGAACCAAATGCTCAGCTGCCATCGAGGGATAGTGGGTCACCAAGAAATCCTCAGCTTTGCAAAATCACAGGTTGATTACATTTTGGGGTCTAACCCATCGAACATGAGCTACTTGGTGGGGTATGGTCCAAACTACCCTCAACGGGTGCACCACAGAGGCGCCTCTGTTGTGTCTTATAGAGAGAACAAAGCGTTCATTGGGTGTACACAAGGGTATGATAACTGGTATGGTAGGGCTGAGCCAAACCCGAATGTTTTGGTTGGGGCTTTGGTTGGAGGGCCCGATTCTCAAGACAATTTTGTGGATCAGAGGGACAATTATATGCAGACTGAGGCCTGTACATATAATACAGCACCATTGGTTGGGGTTTTTGCTAAATTTTCAGAATTAGAGGTCCAAAAGGAGTTGGATCATATTCAGGATTTGGGTTTAGTTGCTTCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

517

Amino Acids

58.52

Weight (kDa)

5.87

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 492 2.3e-168 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1265
AccB7I CCANNNNNTGG 2 cut(s) 366, 1469
AccII CGCG 3 cut(s) 74, 350, 542
AciI CCGC 8 cut(s) 72, 172, 540, 542, 545, 554, 558, 587
AclWI GGATC 3 cut(s) 531, 1419, 1524
AcoI YGGCCR 2 cut(s) 139, 555
AcsI RAATTY 1 cut(s) 1484
AcyI GRCGYC 4 cut(s) 96, 237, 330, 1266
AfaI GTAC 8 cut(s) 152, 494, 857, 919, 953, 1025, 1312, 1447
AfiI CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
AgsI TTSAA 5 cut(s) 85, 125, 574, 718, 869
AhlI ACTAGT 1 cut(s) 398
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 2 cut(s) 167, 293
AleI CACNNNNGTG 1 cut(s) 34
Alw21I GWGCWC 3 cut(s) 342, 348, 1257
Alw26I GTCTC 3 cut(s) 116, 429, 530
Alw44I GTGCAC 1 cut(s) 1253
AlwI GGATC 3 cut(s) 531, 1419, 1524
Ama87I CYCGRG 2 cut(s) 341, 515
ApaI GGGCCC 1 cut(s) 1389
ApaLI GTGCAC 1 cut(s) 1253
ApeKI GCWGC 6 cut(s) 262, 316, 560, 737, 878, 1122
ApoI RAATTY 1 cut(s) 1484
AspLEI GCGC 1 cut(s) 1268
AspS9I GGNCC 7 cut(s) 175, 372, 417, 1232, 1385, 1386, 1501
AsuC2I CCSGG 3 cut(s) 484, 516, 517
AsuHPI GGTGA 8 cut(s) 16, 157, 181, 254, 440, 697, 718, 1136
AvaI CYCGRG 2 cut(s) 341, 515
AvaII GGWCC 4 cut(s) 175, 372, 1232, 1501
BaeGI GKGCMC 2 cut(s) 1257, 1389
BanI GGYRCC 1 cut(s) 1265
BanII GRGCYC 2 cut(s) 342, 1389
BbsI GAAGAC 1 cut(s) 857
Bbv12I GWGCWC 3 cut(s) 342, 348, 1257
BbvCI CCTCAGC 1 cut(s) 1157
BbvI GCAGC 6 cut(s) 249, 328, 572, 749, 865, 1109
BccI CCATC 6 cut(s) 346, 368, 425, 670, 1134, 1210
BceAI ACGGC 2 cut(s) 133, 211
BcgI CGANNNNNNTGC 2 cut(s) 1096, 1130
BciT130I CCWGG 2 cut(s) 169, 295
BcnI CCSGG 3 cut(s) 484, 516, 517
BcoDI GTCTC 3 cut(s) 116, 429, 530
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 2 cut(s) 200, 399
BfmI CTRYAG 1 cut(s) 646
BfoI RGCGCY 1 cut(s) 1269
BlpI GCTNAGC 2 cut(s) 1118, 1344
BmcAI AGTACT 2 cut(s) 919, 953
Bme1390I CCNGG 5 cut(s) 169, 295, 484, 516, 517
Bme18I GGWCC 4 cut(s) 175, 372, 1232, 1501
BmeT110I CYCGRG 2 cut(s) 341, 515
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 7 cut(s) 175, 372, 417, 1232, 1385, 1386, 1501
BmiI GGNNCC 4 cut(s) 176, 373, 1267, 1387
BmrFI CCNGG 5 cut(s) 169, 295, 484, 516, 517
BpiI GAAGAC 1 cut(s) 857
BpmI CTGGAG 1 cut(s) 316
Bpu10I CCTNAGC 1 cut(s) 1157
Bpu1102I GCTNAGC 2 cut(s) 1118, 1344
BpuEI CTTGAG 1 cut(s) 1380
BpuMI CCSGG 3 cut(s) 484, 516, 517
BsaHI GRCGYC 4 cut(s) 96, 237, 330, 1266
BsaI GGTCTC 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 420, 515, 540
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
Bse118I RCCGGY 4 cut(s) 141, 239, 312, 332
Bse1I ACTGG 2 cut(s) 930, 1334
Bse3DI GCAATG 2 cut(s) 555, 1040
BseBI CCWGG 2 cut(s) 169, 295
BseDI CCNNGG 3 cut(s) 420, 515, 540
BseGI GGATG 2 cut(s) 479, 681
BseLI CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
BseMI GCAATG 2 cut(s) 555, 1040
BseMII CTCAG 4 cut(s) 1132, 1171, 1335, 1428
BseNI ACTGG 2 cut(s) 930, 1334
BseRI GAGGAG 3 cut(s) 104, 680, 785
BseSI GKGCMC 2 cut(s) 1257, 1389
BseX3I CGGCCG 2 cut(s) 139, 555
BseXI GCAGC 6 cut(s) 249, 328, 572, 749, 865, 1109
BseYI CCCAGC 1 cut(s) 843
Bsh1236I CGCG 3 cut(s) 74, 350, 542
Bsh1285I CGRYCG 3 cut(s) 72, 142, 558
BshNI GGYRCC 1 cut(s) 1265
BsiEI CGRYCG 3 cut(s) 72, 142, 558
BsiHKAI GWGCWC 3 cut(s) 342, 348, 1257
BsiHKCI CYCGRG 2 cut(s) 341, 515
BslFI GGGAC 6 cut(s) 107, 188, 385, 653, 860, 1433
BslI CCNNNNNNNGG 3 cut(s) 366, 1249, 1469
BsmAI GTCTC 3 cut(s) 116, 429, 530
BsmFI GGGAC 6 cut(s) 107, 188, 385, 653, 860, 1433
Bso31I GGTCTC 1 cut(s) 530
BsoBI CYCGRG 2 cut(s) 341, 515
Bsp120I GGGCCC 1 cut(s) 1385
Bsp1286I GDGCHC 4 cut(s) 342, 348, 1257, 1389
Bsp1407I TGTACA 3 cut(s) 1023, 1310, 1445
Bsp143I GATC 4 cut(s) 523, 938, 1411, 1516
Bsp1720I GCTNAGC 2 cut(s) 1118, 1344
BspACI CCGC 8 cut(s) 72, 172, 540, 542, 545, 554, 558, 587
BspCNI CTCAG 4 cut(s) 1131, 1170, 1336, 1429
BspFNI CGCG 3 cut(s) 74, 350, 542
BspLI GGNNCC 4 cut(s) 176, 373, 1267, 1387
BspPI GGATC 3 cut(s) 531, 1419, 1524
BspQI GCTCTTC 1 cut(s) 683
BspT107I GGYRCC 1 cut(s) 1265
BspTNI GGTCTC 1 cut(s) 530
BsrDI GCAATG 2 cut(s) 555, 1040
BsrFI RCCGGY 4 cut(s) 141, 239, 312, 332
BsrGI TGTACA 3 cut(s) 1023, 1310, 1445
BsrI ACTGG 2 cut(s) 930, 1334
BssAI RCCGGY 4 cut(s) 141, 239, 312, 332
BssECI CCNNGG 3 cut(s) 420, 515, 540
BssMI GATC 4 cut(s) 523, 938, 1411, 1516
BssNI GRCGYC 4 cut(s) 96, 237, 330, 1266
Bst2UI CCWGG 2 cut(s) 169, 295
Bst4CI ACNGT 2 cut(s) 280, 917
Bst6I CTCTTC 2 cut(s) 683, 891
BstACI GRCGYC 4 cut(s) 96, 237, 330, 1266
BstAUI TGTACA 3 cut(s) 1023, 1310, 1445
BstC8I GCNNGC 6 cut(s) 334, 338, 348, 455, 489, 1015
BstDEI CTNAG 5 cut(s) 565, 1118, 1157, 1344, 1437
BstDSI CCRYGG 1 cut(s) 540
BstEII GGTNACC 2 cut(s) 242, 1142
BstF5I GGATG 2 cut(s) 479, 681
BstFNI CGCG 3 cut(s) 74, 350, 542
BstH2I RGCGCY 1 cut(s) 1269
BstHHI GCGC 1 cut(s) 1268
BstKTI GATC 4 cut(s) 526, 941, 1414, 1519
BstMAI GTCTC 3 cut(s) 116, 429, 530
BstMBI GATC 4 cut(s) 523, 938, 1411, 1516
BstMCI CGRYCG 3 cut(s) 72, 142, 558
BstMWI GCNNNNNNNGC 4 cut(s) 356, 551, 554, 560
BstNI CCWGG 2 cut(s) 169, 295
BstNSI RCATGY 1 cut(s) 1048
BstPI GGTNACC 2 cut(s) 242, 1142
BstSCI CCNGG 5 cut(s) 167, 293, 482, 514, 515
BstSFI CTRYAG 1 cut(s) 646
BstSLI GKGCMC 2 cut(s) 1257, 1389
BstUI CGCG 3 cut(s) 74, 350, 542
BstV1I GCAGC 6 cut(s) 249, 328, 572, 749, 865, 1109
BstV2I GAAGAC 1 cut(s) 857
BstX2I RGATCY 1 cut(s) 523
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 1 cut(s) 523
BstZI CGGCCG 2 cut(s) 139, 555
BtgI CCRYGG 1 cut(s) 540
BtgZI GCGATG 1 cut(s) 365
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 2 cut(s) 479, 681
BtsIMutI CAGTG 2 cut(s) 438, 923
Cac8I GCNNGC 6 cut(s) 334, 338, 348, 455, 489, 1015
CciNI GCGGCCGC 1 cut(s) 555
CfoI GCGC 1 cut(s) 1268
Cfr10I RCCGGY 4 cut(s) 141, 239, 312, 332
Cfr13I GGNCC 7 cut(s) 175, 372, 417, 1232, 1385, 1386, 1501
Cfr42I CCGCGG 1 cut(s) 543
Cfr9I CCCGGG 1 cut(s) 515
CseI GACGC 4 cut(s) 104, 245, 338, 690
Csp6I GTAC 8 cut(s) 151, 493, 856, 918, 952, 1024, 1311, 1446
CviAII CATG 6 cut(s) 472, 874, 966, 1045, 1081, 1210
CviQI GTAC 8 cut(s) 151, 493, 856, 918, 952, 1024, 1311, 1446
DdeI CTNAG 5 cut(s) 565, 1118, 1157, 1344, 1437
DinI GGCGCC 1 cut(s) 1267
DpnI GATC 4 cut(s) 525, 940, 1413, 1518
DpnII GATC 4 cut(s) 523, 938, 1411, 1516
EaeI YGGCCR 2 cut(s) 139, 555
EagI CGGCCG 2 cut(s) 139, 555
Eam1104I CTCTTC 2 cut(s) 683, 891
EarI CTCTTC 2 cut(s) 683, 891
Ecl136II GAGCTC 1 cut(s) 340
EclXI CGGCCG 2 cut(s) 139, 555
Eco147I AGGCCT 2 cut(s) 1017, 1442
Eco24I GRGCYC 2 cut(s) 342, 1389
Eco31I GGTCTC 1 cut(s) 530
Eco47I GGWCC 4 cut(s) 175, 372, 1232, 1501
Eco52I CGGCCG 2 cut(s) 139, 555
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 2 cut(s) 341, 515
Eco91I GGTNACC 2 cut(s) 242, 1142
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 1385
EcoO65I GGTNACC 2 cut(s) 242, 1142
EcoRII CCWGG 2 cut(s) 167, 293
EcoT38I GRGCYC 2 cut(s) 342, 1389
EgeI GGCGCC 1 cut(s) 1267
EheI GGCGCC 1 cut(s) 1267
FaeI CATG 6 cut(s) 475, 877, 969, 1048, 1084, 1213
FaqI GGGAC 6 cut(s) 107, 188, 385, 653, 860, 1433
FatI CATG 6 cut(s) 471, 873, 965, 1044, 1080, 1209
FauI CCCGC 2 cut(s) 165, 594
FokI GGATG 2 cut(s) 466, 688
FriOI GRGCYC 2 cut(s) 342, 1389
FspBI CTAG 2 cut(s) 200, 399
GlaI GCGC 1 cut(s) 1267
GsaI CCCAGC 1 cut(s) 847
GsuI CTGGAG 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 1269
HgaI GACGC 4 cut(s) 104, 245, 338, 690
HhaI GCGC 1 cut(s) 1268
Hin1I GRCGYC 4 cut(s) 96, 237, 330, 1266
Hin1II CATG 6 cut(s) 475, 877, 969, 1048, 1084, 1213
Hin6I GCGC 1 cut(s) 1266
HinP1I GCGC 1 cut(s) 1266
HinfI GANTC 4 cut(s) 109, 125, 162, 1391
HphI GGTGA 8 cut(s) 16, 157, 181, 254, 440, 697, 718, 1136
Hpy166II GTNNAC 5 cut(s) 214, 276, 1255, 1311, 1313
Hpy188I TCNGA 4 cut(s) 523, 1090, 1416, 1492
Hpy188III TCNNGA 4 cut(s) 67, 574, 1397, 1526
Hpy8I GTNNAC 5 cut(s) 214, 276, 1255, 1311, 1313
Hpy99I CGWCG 3 cut(s) 197, 332, 706
HpyAV CCTTC 2 cut(s) 611, 1082
HpyCH4III ACNGT 2 cut(s) 280, 917
HpyCH4IV ACGT 2 cut(s) 249, 849
HpyCH4V TGCA 6 cut(s) 29, 881, 1048, 1166, 1255, 1432
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 551, 554, 560
HpyF3I CTNAG 5 cut(s) 565, 1118, 1157, 1344, 1437
HpySE526I ACGT 2 cut(s) 249, 849
Hsp92I GRCGYC 4 cut(s) 96, 237, 330, 1266
Hsp92II CATG 6 cut(s) 475, 877, 969, 1048, 1084, 1213
HspAI GCGC 1 cut(s) 1266
KasI GGCGCC 1 cut(s) 1265
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
KspI CCGCGG 1 cut(s) 543
Kzo9I GATC 4 cut(s) 523, 938, 1411, 1516
LguI GCTCTTC 1 cut(s) 683
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 6 cut(s) 249, 328, 572, 749, 865, 1109
MaeI CTAG 2 cut(s) 200, 399
MaeII ACGT 2 cut(s) 249, 849
MaeIII GTNAC 4 cut(s) 163, 242, 280, 1142
MalI GATC 4 cut(s) 525, 940, 1413, 1518
MboI GATC 4 cut(s) 523, 938, 1411, 1516
MboII GAAGA 5 cut(s) 19, 46, 700, 857, 908
MfeI CAATTG 1 cut(s) 623
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 4 cut(s) 342, 348, 1257, 1389
MluCI AATT 7 cut(s) 85, 623, 789, 1402, 1423, 1484, 1493
Mly113I GGCGCC 1 cut(s) 1266
MlyI GAGTC 2 cut(s) 118, 171
MmeI TCCRAC 3 cut(s) 501, 1360, 1494
MreI CGCCGGCG 1 cut(s) 332
MroNI GCCGGC 1 cut(s) 332
MseI TTAA 2 cut(s) 792, 852
MslI CAYNNNNRTG 1 cut(s) 34
MspA1I CMGCKG 3 cut(s) 542, 843, 1122
MspR9I CCNGG 5 cut(s) 169, 295, 484, 516, 517
MunI CAATTG 1 cut(s) 623
MvaI CCWGG 2 cut(s) 169, 295
MvnI CGCG 3 cut(s) 74, 350, 542
MwoI GCNNNNNNNGC 4 cut(s) 356, 551, 554, 560
NaeI GCCGGC 1 cut(s) 334
NarI GGCGCC 1 cut(s) 1266
NciI CCSGG 3 cut(s) 484, 516, 517
NdeII GATC 4 cut(s) 523, 938, 1411, 1516
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 6 cut(s) 475, 877, 969, 1048, 1084, 1213
NlaIV GGNNCC 4 cut(s) 176, 373, 1267, 1387
NmeAIII GCCGAG 2 cut(s) 445, 973
NmuCI GTSAC 4 cut(s) 163, 242, 280, 1142
NotI GCGGCCGC 1 cut(s) 555
NspI RCATGY 1 cut(s) 1048
OliI CACNNNNGTG 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 341
PceI AGGCCT 2 cut(s) 1017, 1442
PciSI GCTCTTC 1 cut(s) 683
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 2 cut(s) 125, 1391
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 2 cut(s) 366, 1469
PfoI TCCNGGA 1 cut(s) 293
PleI GAGTC 2 cut(s) 117, 170
PluTI GGCGCC 1 cut(s) 1269
PpsI GAGTC 2 cut(s) 117, 170
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 167, 293
PspEI GGTNACC 2 cut(s) 242, 1142
PspFI CCCAGC 1 cut(s) 843
PspGI CCWGG 2 cut(s) 167, 293
PspN4I GGNNCC 4 cut(s) 176, 373, 1267, 1387
PspOMI GGGCCC 1 cut(s) 1385
PspPI GGNCC 7 cut(s) 175, 372, 417, 1232, 1385, 1386, 1501
PspXI VCTCGAGB 1 cut(s) 341
PsrI GAACNNNNNNTAC 2 cut(s) 1199, 1231
PsuI RGATCY 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 2 cut(s) 843, 1122
RsaI GTAC 8 cut(s) 152, 494, 857, 919, 953, 1025, 1312, 1447
RsaNI GTAC 8 cut(s) 151, 493, 856, 918, 952, 1024, 1311, 1446
RseI CAYNNNNRTG 1 cut(s) 34
SacI GAGCTC 1 cut(s) 342
SacII CCGCGG 1 cut(s) 543
SapI GCTCTTC 1 cut(s) 683
SaqAI TTAA 2 cut(s) 792, 852
Sau3AI GATC 4 cut(s) 523, 938, 1411, 1516
Sau96I GGNCC 7 cut(s) 175, 372, 417, 1232, 1385, 1386, 1501
ScaI AGTACT 2 cut(s) 919, 953
SchI GAGTC 2 cut(s) 118, 171
ScrFI CCNGG 5 cut(s) 169, 295, 484, 516, 517
SduI GDGCHC 4 cut(s) 342, 348, 1257, 1389
SfcI CTRYAG 1 cut(s) 646
SfoI GGCGCC 1 cut(s) 1267
Sfr274I CTCGAG 1 cut(s) 341
Sfr303I CCGCGG 1 cut(s) 543
SgrAI CRCCGGYG 2 cut(s) 239, 332
SgrBI CCGCGG 1 cut(s) 543
SinI GGWCC 4 cut(s) 175, 372, 1232, 1501
SlaI CTCGAG 1 cut(s) 341
SmaI CCCGGG 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 2 cut(s) 341, 1395
SmoI CTYRAG 2 cut(s) 341, 1395
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 7 cut(s) 85, 623, 789, 1402, 1423, 1484, 1493
SseBI AGGCCT 2 cut(s) 1017, 1442
SsiI CCGC 8 cut(s) 72, 172, 540, 542, 545, 554, 558, 587
SspDI GGCGCC 1 cut(s) 1265
SspMI CTAG 2 cut(s) 200, 399
SstI GAGCTC 1 cut(s) 342
StuI AGGCCT 2 cut(s) 1017, 1442
StyD4I CCNGG 5 cut(s) 167, 293, 482, 514, 515
TaaI ACNGT 2 cut(s) 280, 917
TaiI ACGT 2 cut(s) 252, 852
TaqI TCGA 6 cut(s) 342, 630, 941, 1106, 1129, 1205
TaqII GACCGA 1 cut(s) 389
TasI AATT 7 cut(s) 85, 623, 789, 1402, 1423, 1484, 1493
TatI WGTACW 5 cut(s) 917, 951, 1023, 1310, 1445
TauI GCSGC 4 cut(s) 545, 548, 557, 560
TfiI GAWTC 2 cut(s) 125, 1391
Tru1I TTAA 2 cut(s) 792, 852
Tru9I TTAA 2 cut(s) 792, 852
TscAI CASTG 2 cut(s) 445, 930
TseFI GTSAC 4 cut(s) 163, 242, 280, 1142
TseI GCWGC 6 cut(s) 262, 316, 560, 737, 878, 1122
Tsp45I GTSAC 4 cut(s) 163, 242, 280, 1142
TspDTI ATGAA 5 cut(s) 373, 909, 954, 1056, 1291
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 2 cut(s) 445, 930
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 2 cut(s) 366, 1469
VneI GTGCAC 1 cut(s) 1253
VpaK11BI GGWCC 4 cut(s) 175, 372, 1232, 1501
XapI RAATTY 1 cut(s) 1484
XceI RCATGY 1 cut(s) 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XhoI CTCGAG 1 cut(s) 341
XmaI CCCGGG 1 cut(s) 515
XspI CTAG 2 cut(s) 200, 399
ZrmI AGTACT 2 cut(s) 919, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.