AT2G33740

CutA1 divalent ion tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006754
Physical Location & Seq
Forward (+)
14269461 .. 14271723
2263 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33740.1

Sequence Viewer

Length: 471 bp
ATGGCTTCGTCTCTCACCACTAGATTATCGGCGGTAATCGGATCTCGACGGAGTTTTCCGATCGTCGGAGCGTTTTGCGTTCTGTCAACTCTCTCAATCTCTTCTCTTTCTTCTTCATCTCCCTTCAAATCGGGTTGTGCACAGTCTTTCTCTGTTGTTCCTCTCTTAAGGTCTAAGTTTAGCAGTAAGGCTTTTTCTTCGTCGATCAGAATGGAGGAGAGCAGCAAAACTGTGCCCAGCATTGTTGTCTATGTCACTGTTCCTAACAGAGAAGCAGGGAAGAAGTTGGCTAACAGCATTGTCCAGGAAAAGCTTGCAGCGTGTGTGAACATTGTGCCTGGCATTGAATCGGTGTACGAGTGGGAGGGGAAGGTTCAGAGTGATTCAGAGGAGCTCCTGATAATCAAAACAAGGCAATCCCTTCTAGAACCTCTAACCGAGCATGTCAATGCAAATCACGAATACGAGTAA

Protein Analysis

156

Amino Acids

16.9

Weight (kDa)

8.48

Isoelectric Point (pI)

63.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CutA1 PF03091 81 - 156 1.2e-26 CutA1 divalent ion tolerance protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 32
AclWI GGATC 1 cut(s) 49
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 65
AflII CTTAAG 1 cut(s) 166
AgsI TTSAA 2 cut(s) 127, 347
AjnI CCWGG 2 cut(s) 303, 337
AluBI AGCT 2 cut(s) 313, 394
AluI AGCT 2 cut(s) 313, 394
Alw21I GWGCWC 2 cut(s) 142, 396
Alw26I GTCTC 1 cut(s) 15
Alw44I GTGCAC 1 cut(s) 138
AlwI GGATC 1 cut(s) 49
ApaLI GTGCAC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 222, 317
AsuHPI GGTGA 1 cut(s) 7
BaeGI GKGCMC 2 cut(s) 142, 237
BanII GRGCYC 1 cut(s) 396
Bbv12I GWGCWC 2 cut(s) 142, 396
BbvI GCAGC 2 cut(s) 234, 329
BciT130I CCWGG 2 cut(s) 305, 339
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 2 cut(s) 21, 425
BfrI CTTAAG 1 cut(s) 166
BisI GCNGC 2 cut(s) 223, 318
BlsI GCNGC 2 cut(s) 224, 319
Bme1390I CCNGG 2 cut(s) 305, 339
BmrFI CCNGG 2 cut(s) 305, 339
Bsc4I CCNNNNNNNGG 1 cut(s) 65
BseBI CCWGG 2 cut(s) 305, 339
BseLI CCNNNNNNNGG 1 cut(s) 65
BseRI GAGGAG 2 cut(s) 230, 404
BseSI GKGCMC 2 cut(s) 142, 237
BseXI GCAGC 2 cut(s) 234, 329
BseYI CCCAGC 1 cut(s) 236
Bsh1285I CGRYCG 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 142, 396
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 15
BsmBI CGTCTC 1 cut(s) 15
Bsp1286I GDGCHC 3 cut(s) 142, 237, 396
Bsp143I GATC 3 cut(s) 41, 60, 204
BspACI CCGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 49
BspTI CTTAAG 1 cut(s) 166
BssMI GATC 3 cut(s) 41, 60, 204
Bst2UI CCWGG 2 cut(s) 305, 339
Bst4CI ACNGT 3 cut(s) 144, 232, 259
Bst6I CTCTTC 1 cut(s) 106
BstAFI CTTAAG 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 1 cut(s) 174
BstKTI GATC 3 cut(s) 44, 63, 207
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 3 cut(s) 41, 60, 204
BstMCI CGRYCG 1 cut(s) 63
BstNI CCWGG 2 cut(s) 305, 339
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 2 cut(s) 303, 337
BstSLI GKGCMC 2 cut(s) 142, 237
BstV1I GCAGC 2 cut(s) 234, 329
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 315
Csp6I GTAC 1 cut(s) 355
CviAII CATG 1 cut(s) 443
CviJI RGCY 5 cut(s) 5, 191, 290, 313, 394
CviKI_1 RGCY 5 cut(s) 5, 191, 290, 313, 394
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 1 cut(s) 174
DpnI GATC 3 cut(s) 43, 62, 206
DpnII GATC 3 cut(s) 41, 60, 204
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 396
Eco53kI GAGCTC 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 303, 337
EcoT38I GRGCYC 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 1 cut(s) 446
FaiI YATR 2 cut(s) 252, 444
FatI CATG 1 cut(s) 442
Fnu4HI GCNGC 2 cut(s) 223, 318
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 2 cut(s) 223, 318
FspBI CTAG 2 cut(s) 21, 425
GluI GCNGC 2 cut(s) 223, 318
GsaI CCCAGC 1 cut(s) 240
Hin1II CATG 1 cut(s) 446
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 2 cut(s) 347, 383
HphI GGTGA 1 cut(s) 7
Hpy166II GTNNAC 4 cut(s) 87, 140, 328, 355
Hpy188I TCNGA 6 cut(s) 41, 60, 68, 209, 378, 388
Hpy188III TCNNGA 4 cut(s) 45, 397, 425, 458
Hpy8I GTNNAC 4 cut(s) 87, 140, 328, 355
Hpy99I CGWCG 3 cut(s) 51, 68, 205
HpyAV CCTTC 3 cut(s) 133, 364, 431
HpyCH4III ACNGT 3 cut(s) 144, 232, 259
HpyCH4V TGCA 3 cut(s) 140, 317, 452
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 1 cut(s) 446
Kzo9I GATC 3 cut(s) 41, 60, 204
LmnI GCTCC 3 cut(s) 68, 391, 399
LpnPI CCDG 7 cut(s) 250, 261, 290, 317, 324, 351, 410
Lsp1109I GCAGC 2 cut(s) 234, 329
MaeI CTAG 2 cut(s) 21, 425
MaeIII GTNAC 1 cut(s) 253
MalI GATC 3 cut(s) 43, 62, 206
MboI GATC 3 cut(s) 41, 60, 204
MboII GAAGA 5 cut(s) 93, 102, 105, 189, 292
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 3 cut(s) 142, 237, 396
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 5 cut(s) 171, 208, 358, 382, 441
MseI TTAA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 166
MspR9I CCNGG 2 cut(s) 305, 339
MvaI CCWGG 2 cut(s) 305, 339
NdeII GATC 3 cut(s) 41, 60, 204
NlaIII CATG 1 cut(s) 446
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 446
PfeI GAWTC 2 cut(s) 347, 383
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 2 cut(s) 224, 319
Ple19I CGATCG 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 303, 337
PspFI CCCAGC 1 cut(s) 236
PspGI CCWGG 2 cut(s) 303, 337
PsuI RGATCY 1 cut(s) 41
PvuI CGATCG 1 cut(s) 63
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 447
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 2 cut(s) 223, 318
Sau3AI GATC 3 cut(s) 41, 60, 204
ScrFI CCNGG 2 cut(s) 305, 339
SduI GDGCHC 3 cut(s) 142, 237, 396
SetI ASST 5 cut(s) 173, 315, 375, 396, 433
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SsiI CCGC 1 cut(s) 32
SspMI CTAG 2 cut(s) 21, 425
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 303, 337
TaaI ACNGT 3 cut(s) 144, 232, 259
TaqI TCGA 2 cut(s) 46, 203
TfiI GAWTC 2 cut(s) 347, 383
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 2 cut(s) 222, 317
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 105
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 262
Vha464I CTTAAG 1 cut(s) 166
VneI GTGCAC 1 cut(s) 138
XbaI TCTAGA 1 cut(s) 424
XceI RCATGY 1 cut(s) 446
XspI CTAG 2 cut(s) 21, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.