MD08G1193500.v1.1

Protein CutA, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006754
Physical Location & Seq
Forward (+)
24905281 .. 24908325
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1193500.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCTTCTACACTCTGCTGCAGAGCCGACGCCCTTCGAACCATCTCCTCCTCCGCCACAATTCGACGCCGTCTTCCCCTGGTGGGAGCATTCTGCGTATTCACCTTGGGCATCTCCAACCTCCTTCCCACTCTCTCCTTCTCCGCCTTCAGAACGGGCACCGCTCAGTCTCTCCCTTTTGCCCCTTTCTTGAGATCAAAGTTTGGCAGCCGATCAGCAGGCGTTGGTTCTGTGAGAATGGAAGGGAGTAGCGATACTACTGTTCCAAGCATTGTTGTTTATGTTACTGTGCCCAGCAGAGAAGTAGGAAAGAAGTTGGCTGAAAGCTTAGTCAGAGAAAAACTTGCAGCCTGTGTTAACCTAGTGCCAGGCATTGAGTCCGTCTATGAGTGGAAAGGAGAGGTCCAGACAGATTCTGAAGAACTTCTTATAATCAAGACAAGGCAATCCCTTTTTGAAGCTTTGACTGCGCATGTCAAGGCAAACCATCCATACGATGTTCCGGAAGTTATTGCCTTGCCTATCAACGCAGGCAGTCTCCCGTACCTAGAATGGATCAAAAACAGCACAAGGGACTGA

Protein Analysis

193

Amino Acids

20.83

Weight (kDa)

8.98

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CutA1 PF03091 91 - 188 1.7e-38 CutA1 divalent ion tolerance protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
Acc16I TGCGCA 1 cut(s) 471
AccB1I GGYRCC 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 164
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 3 cut(s) 54, 144, 162
AclWI GGATC 1 cut(s) 563
AcuI CTGAAG 2 cut(s) 133, 438
AcyI GRCGYC 2 cut(s) 30, 67
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 1 cut(s) 458
AjnI CCWGG 2 cut(s) 78, 367
AluBI AGCT 2 cut(s) 327, 461
AluI AGCT 2 cut(s) 327, 461
Alw26I GTCTC 2 cut(s) 174, 542
AlwI GGATC 1 cut(s) 563
AlwNI CAGNNNCTG 1 cut(s) 416
Aor13HI TCCGGA 1 cut(s) 502
ApeKI GCWGC 3 cut(s) 18, 207, 347
Asp700I GAANNNNTTC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 403
BaeGI GKGCMC 2 cut(s) 161, 294
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 5, 219, 359
BccI CCATC 2 cut(s) 50, 495
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 2 cut(s) 80, 369
BcoDI GTCTC 2 cut(s) 174, 542
BfaI CTAG 2 cut(s) 362, 548
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 3 cut(s) 19, 208, 348
BlsI GCNGC 3 cut(s) 20, 209, 349
Bme1390I CCNGG 2 cut(s) 80, 369
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 80, 369
BmsI GCATC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 211
BsaHI GRCGYC 2 cut(s) 30, 67
BsaJI CCNNGG 2 cut(s) 78, 105
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 2 cut(s) 80, 369
BseDI CCNNGG 2 cut(s) 78, 105
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 179
BseRI GAGGAG 2 cut(s) 37, 40
BseSI GKGCMC 2 cut(s) 161, 294
BseXI GCAGC 3 cut(s) 5, 219, 359
BseYI CCCAGC 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 158
BsiSI CCGG 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 174, 542
BsmI GAATGC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 161, 294
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 3 cut(s) 194, 212, 555
BspACI CCGC 3 cut(s) 54, 144, 162
BspCNI CTCAG 1 cut(s) 178
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 160
BspMAI CTGCAG 1 cut(s) 23
BspPI GGATC 1 cut(s) 563
BspT104I TTCGAA 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 78, 105
BssMI GATC 3 cut(s) 194, 212, 555
BssNI GRCGYC 2 cut(s) 30, 67
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 80, 369
Bst4CI ACNGT 2 cut(s) 262, 289
BstACI GRCGYC 2 cut(s) 30, 67
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 220, 532
BstDEI CTNAG 2 cut(s) 165, 328
BstF5I GGATG 1 cut(s) 487
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 3 cut(s) 197, 215, 558
BstMAI GTCTC 2 cut(s) 174, 542
BstMBI GATC 3 cut(s) 194, 212, 555
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstNI CCWGG 2 cut(s) 80, 369
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 78, 367
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 2 cut(s) 161, 294
BstV1I GCAGC 3 cut(s) 5, 219, 359
BstV2I GAAGAC 1 cut(s) 65
BtsCI GGATG 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 220, 532
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 472
Cfr13I GGNCC 1 cut(s) 403
CseI GACGC 2 cut(s) 38, 75
Csp6I GTAC 1 cut(s) 544
CviAII CATG 1 cut(s) 473
CviJI RGCY 7 cut(s) 5, 26, 210, 320, 327, 350, 461
CviKI_1 RGCY 7 cut(s) 5, 26, 210, 320, 327, 350, 461
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 2 cut(s) 165, 328
DpnI GATC 3 cut(s) 196, 214, 557
DpnII GATC 3 cut(s) 194, 212, 555
EciI GGCGGA 2 cut(s) 43, 133
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 2 cut(s) 133, 438
EcoRII CCWGG 2 cut(s) 78, 367
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 1 cut(s) 476
FaiI YATR 5 cut(s) 282, 387, 431, 474, 493
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 19, 208, 348
FokI GGATG 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 19, 208, 348
FspBI CTAG 2 cut(s) 362, 548
FspI TGCGCA 1 cut(s) 471
GlaI GCGC 1 cut(s) 471
GluI GCNGC 3 cut(s) 19, 208, 348
GsaI CCCAGC 1 cut(s) 297
HapII CCGG 1 cut(s) 503
HgaI GACGC 2 cut(s) 38, 75
HhaI GCGC 1 cut(s) 472
Hin1I GRCGYC 2 cut(s) 30, 67
Hin1II CATG 1 cut(s) 476
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 2 cut(s) 325, 459
HinfI GANTC 2 cut(s) 377, 413
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 1 cut(s) 503
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 3 cut(s) 152, 335, 418
Hpy188III TCNNGA 4 cut(s) 190, 406, 436, 503
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 2 cut(s) 32, 69
HpyAV CCTTC 5 cut(s) 44, 134, 148, 157, 236
HpyCH4III ACNGT 2 cut(s) 262, 289
HpyCH4V TGCA 2 cut(s) 21, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 2 cut(s) 165, 328
Hsp92I GRCGYC 2 cut(s) 30, 67
Hsp92II CATG 1 cut(s) 476
HspAI GCGC 1 cut(s) 470
Kpn2I TCCGGA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 3 cut(s) 194, 212, 555
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 3 cut(s) 5, 219, 359
LweI GCATC 1 cut(s) 120
MaeI CTAG 2 cut(s) 362, 548
MaeIII GTNAC 1 cut(s) 283
MalI GATC 3 cut(s) 196, 214, 557
MbiI CCGCTC 1 cut(s) 164
MboI GATC 3 cut(s) 194, 212, 555
MboII GAAGA 2 cut(s) 65, 431
MhlI GDGCHC 2 cut(s) 161, 294
MluCI AATT 1 cut(s) 60
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 4 cut(s) 58, 61, 131, 394
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 423
MseI TTAA 1 cut(s) 357
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 2 cut(s) 80, 369
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 2 cut(s) 80, 369
MwoI GCNNNNNNNGC 1 cut(s) 467
NdeII GATC 3 cut(s) 194, 212, 555
NlaIII CATG 1 cut(s) 476
NlaIV GGNNCC 1 cut(s) 160
NsbI TGCGCA 1 cut(s) 471
NspI RCATGY 1 cut(s) 476
NspV TTCGAA 1 cut(s) 37
PctI GAATGC 1 cut(s) 89
PdmI GAANNNNTTC 1 cut(s) 423
PfeI GAWTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 69
PkrI GCNGC 3 cut(s) 20, 209, 349
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PsiI TTATAA 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 78, 367
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 2 cut(s) 78, 367
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 403
PstI CTGCAG 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 416
PsyI GACNNNGTC 1 cut(s) 69
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 3 cut(s) 19, 208, 348
Sau3AI GATC 3 cut(s) 194, 212, 555
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 80, 369
SduI GDGCHC 2 cut(s) 161, 294
SetI ASST 7 cut(s) 107, 123, 329, 363, 405, 463, 549
SfaNI GCATC 1 cut(s) 120
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 37
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 1 cut(s) 60
SsiI CCGC 3 cut(s) 54, 144, 162
SspMI CTAG 2 cut(s) 362, 548
StyD4I CCNGG 2 cut(s) 78, 367
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 262, 289
TaqI TCGA 2 cut(s) 37, 64
TasI AATT 1 cut(s) 60
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseI GCWGC 3 cut(s) 18, 207, 347
TspGWI ACGGA 1 cut(s) 370
Tth111I GACNNNGTC 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 403
XceI RCATGY 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 423
XspI CTAG 2 cut(s) 362, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.