RLG00000001001

Protein CutA, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006754
Physical Location & Seq
Forward (+)
9101613 .. 9104207
2595 bp
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UTR
Exon/CDS
Intron
RLM00000001001

Sequence Viewer

Length: 555 bp
ATGGCTTCCACTTCCACACTCTTCTGCAGAGCCAAAGCAATACTTCCCTTCTCCTCCACGCTACGACGTCGTTTGCCTCTCGTCGGAGCAATCTGCGTACTCGGCTTCGGCCTCCCCAATCTCTTCTCCGCCGCCTGCAGAACCCGCTGCGCTCGGTCTCTCCCTTTTGCCCCTCTCTTGAGATCAAAATTCAGTAGCCAAGCAAAGGCAATGGAAGGGGCTCAAGGCAGCAGCACTGTACCAAGCATCGTTGTTTATGTCACTGTACCCAACAAAGAAGTAGGAAAGAAGTTAGCTGAAAGCTTGGTCAGAGAAAAACTTGCAGCATGTGTTAACATAGTACCAGGCATTCTGTCCATCTATCAGTGGGAAGGTGAGGTTCAGACAGATTCAGAGGAGCTTCTTATAATCAAGACCAGGCAATCCCTTTTTGAAGCTCTAACAGAGCATGTCAAGACAAACCACCCATATGATGTCCCAGAAGTTATTGCCTTGCCCATCAACGCGGGCAGTCTCAGCTACTTAGAATGGATCAAGAACAGCACAAAGGACTGA

Protein Analysis

185

Amino Acids

20.1

Weight (kDa)

9.18

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CutA1 PF03091 83 - 180 1.5e-38 CutA1 divalent ion tolerance protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 407
AatII GACGTC 1 cut(s) 70
AccII CGCG 1 cut(s) 506
AciI CCGC 4 cut(s) 129, 132, 145, 506
AclWI GGATC 1 cut(s) 539
AcsI RAATTY 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 4 cut(s) 99, 240, 267, 342
AfiI CCNNNNNNNGG 2 cut(s) 83, 205
AgsI TTSAA 1 cut(s) 434
AjnI CCWGG 2 cut(s) 343, 416
AluBI AGCT 5 cut(s) 296, 303, 400, 437, 519
AluI AGCT 5 cut(s) 296, 303, 400, 437, 519
Alw26I GTCTC 2 cut(s) 162, 518
AlwI GGATC 1 cut(s) 539
AoxI GGCC 1 cut(s) 109
ApeKI GCWGC 4 cut(s) 147, 228, 231, 323
ApoI RAATTY 1 cut(s) 188
AspLEI GCGC 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 223
BbvI GCAGC 4 cut(s) 134, 240, 243, 335
BccI CCATC 2 cut(s) 365, 506
BciT130I CCWGG 2 cut(s) 345, 418
BcoDI GTCTC 2 cut(s) 162, 518
BfmI CTRYAG 2 cut(s) 25, 136
BisI GCNGC 5 cut(s) 132, 148, 229, 232, 324
BlsI GCNGC 5 cut(s) 133, 149, 230, 233, 325
Bme1390I CCNGG 2 cut(s) 345, 418
BmrFI CCNGG 2 cut(s) 345, 418
BmsI GCATC 1 cut(s) 255
BpuEI CTTGAG 2 cut(s) 199, 207
BsaHI GRCGYC 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 162
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 205
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 2 cut(s) 345, 418
BseLI CCNNNNNNNGG 2 cut(s) 83, 205
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 529
BseRI GAGGAG 2 cut(s) 43, 410
BseXI GCAGC 4 cut(s) 134, 240, 243, 335
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 1 cut(s) 111
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 2 cut(s) 83, 205
BsmAI GTCTC 2 cut(s) 162, 518
BsmFI GGGAC 1 cut(s) 461
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 111
Bso31I GGTCTC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 2 cut(s) 182, 531
BspACI CCGC 4 cut(s) 129, 132, 145, 506
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 1 cut(s) 528
BspFNI CGCG 1 cut(s) 506
BspMAI CTGCAG 2 cut(s) 29, 140
BspPI GGATC 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 216
BssMI GATC 2 cut(s) 182, 531
BssNI GRCGYC 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 345, 418
Bst4CI ACNGT 2 cut(s) 238, 265
Bst6I CTCTTC 2 cut(s) 26, 128
BstACI GRCGYC 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 136, 508
BstDEI CTNAG 2 cut(s) 515, 523
BstFNI CGCG 1 cut(s) 506
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 2 cut(s) 185, 534
BstMAI GTCTC 2 cut(s) 162, 518
BstMBI GATC 2 cut(s) 182, 531
BstMWI GCNNNNNNNGC 3 cut(s) 102, 144, 516
BstNI CCWGG 2 cut(s) 345, 418
BstNSI RCATGY 2 cut(s) 330, 452
BstSCI CCNGG 2 cut(s) 343, 416
BstSFI CTRYAG 2 cut(s) 25, 136
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 4 cut(s) 134, 240, 243, 335
BsuRI GGCC 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 234, 261, 371
Cac8I GCNNGC 2 cut(s) 136, 508
CfoI GCGC 1 cut(s) 152
Csp6I GTAC 4 cut(s) 98, 239, 266, 341
CviAII CATG 2 cut(s) 327, 449
CviQI GTAC 4 cut(s) 98, 239, 266, 341
DdeI CTNAG 2 cut(s) 515, 523
DpnI GATC 2 cut(s) 184, 533
DpnII GATC 2 cut(s) 182, 531
Eam1104I CTCTTC 2 cut(s) 26, 128
EarI CTCTTC 2 cut(s) 26, 128
EciI GGCGGA 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 343, 416
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 2 cut(s) 330, 452
FaiI YATR 7 cut(s) 258, 328, 338, 407, 450, 469, 471
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 461
FatI CATG 2 cut(s) 326, 448
FauI CCCGC 2 cut(s) 152, 499
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 5 cut(s) 132, 148, 229, 232, 324
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 5 cut(s) 132, 148, 229, 232, 324
GlaI GCGC 1 cut(s) 151
GluI GCNGC 5 cut(s) 132, 148, 229, 232, 324
HaeIII GGCC 1 cut(s) 111
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 2 cut(s) 330, 452
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 1 cut(s) 389
HpaI GTTAAC 1 cut(s) 334
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 86, 311, 384, 394
Hpy188III TCNNGA 4 cut(s) 178, 412, 454, 535
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 3 cut(s) 69, 72, 86
HpyAV CCTTC 3 cut(s) 58, 209, 365
HpyCH4III ACNGT 2 cut(s) 238, 265
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 3 cut(s) 27, 138, 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 144, 516
HpyF3I CTNAG 2 cut(s) 515, 523
HpySE526I ACGT 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 2 cut(s) 330, 452
HspAI GCGC 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 2 cut(s) 182, 531
LmnI GCTCC 2 cut(s) 86, 397
LpnPI CCDG 6 cut(s) 148, 330, 357, 403, 430, 492
Lsp1109I GCAGC 4 cut(s) 134, 240, 243, 335
LweI GCATC 1 cut(s) 255
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 1 cut(s) 259
MalI GATC 2 cut(s) 184, 533
MboI GATC 2 cut(s) 182, 531
MboII GAAGA 2 cut(s) 13, 115
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 6 cut(s) 64, 87, 122, 183, 370, 388
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 468
MspA1I CMGCKG 1 cut(s) 147
MspR9I CCNGG 2 cut(s) 345, 418
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 2 cut(s) 345, 418
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 3 cut(s) 102, 144, 516
NdeI CATATG 1 cut(s) 469
NdeII GATC 2 cut(s) 182, 531
NlaIII CATG 2 cut(s) 330, 452
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 2 cut(s) 330, 452
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 389
PkrI GCNGC 5 cut(s) 133, 149, 230, 233, 325
PsiI TTATAA 1 cut(s) 407
Psp6I CCWGG 2 cut(s) 343, 416
PspGI CCWGG 2 cut(s) 343, 416
PstI CTGCAG 2 cut(s) 29, 140
RsaI GTAC 4 cut(s) 99, 240, 267, 342
RsaNI GTAC 4 cut(s) 98, 239, 266, 341
RseI CAYNNNNRTG 1 cut(s) 468
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 5 cut(s) 132, 148, 229, 232, 324
Sau3AI GATC 2 cut(s) 182, 531
ScrFI CCNGG 2 cut(s) 345, 418
SduI GDGCHC 1 cut(s) 223
SetI ASST 8 cut(s) 70, 298, 305, 376, 381, 402, 439, 521
SfaNI GCATC 1 cut(s) 255
SfcI CTRYAG 2 cut(s) 25, 136
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 2 cut(s) 178, 222
SmoI CTYRAG 2 cut(s) 178, 222
Sse9I AATT 1 cut(s) 188
SsiI CCGC 4 cut(s) 129, 132, 145, 506
StyD4I CCNGG 2 cut(s) 343, 416
TaaI ACNGT 2 cut(s) 238, 265
TaiI ACGT 1 cut(s) 70
TaqII GACCGA 1 cut(s) 144
TasI AATT 1 cut(s) 188
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 3 cut(s) 241, 268, 371
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 4 cut(s) 147, 228, 231, 323
Tsp45I GTSAC 1 cut(s) 259
TspRI CASTG 3 cut(s) 241, 268, 371
XapI RAATTY 1 cut(s) 188
XceI RCATGY 2 cut(s) 330, 452
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.