FvH4_5g35790

Protein CutA, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006754
Physical Location & Seq
Reverse (-)
26199245 .. 26202170
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35790.t6

Sequence Viewer

Length: 591 bp
ATGGCTTCCAGACTCTGCTTCAGAGCCAAAGCAATACCTTCCTTCTCCTTTTCCTCATCCTCAACGATACGACGTCGTTTGCCACTCGTCGGAGCATTCTGCGTGCTCAGCTTCGGCCTCCCCAGTCTCTTCTCCGCTGCGTGCAGAACAACCAGCTGCGCGCCTCACTCTCTCCCTTTTGCCCCTCTCTTGAGGGAATTGTGGGTTTTCAGATCAAACTTTGGTAGCCAAGCAAAGGCAATGGAAGGGCCTCAAGGGAGTAGCAGCACCACTGTACCAAGCATTGTTGTCTATGTCACTGTACCCAACAAAGAAGTAGGAAAGAAGTTAGCTGAAAGCTTGGTCAGAGAAAAACTTGCAGCATGTGTAAACATAGTACCAGGCATTCAGTCTGTGTATCAGTGGGAAGGTGAGGTACAGACAGATTCAGAGGAGCTTCTTATAATCAAGACCAGACAATCCCTTTTTGAAGCTCTCACAGAGCATGTCAAGTCAAACCATCCATATGATGTCCCAGAAGTTATTGCCTTGCCCATCAACGCAGGCAGTCTCAACTACCTAGAATGGATCAAGAACAGCACAAGGGACTGA

Protein Analysis

197

Amino Acids

21.53

Weight (kDa)

8.87

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CutA1 PF03091 95 - 140 1e-15 CutA1 divalent ion tolerance protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
AatII GACGTC 1 cut(s) 76
AccII CGCG 1 cut(s) 161
AciI CCGC 1 cut(s) 135
AclWI GGATC 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 73
AfaI GTAC 4 cut(s) 276, 303, 378, 417
AfiI CCNNNNNNNGG 2 cut(s) 89, 235
AgsI TTSAA 1 cut(s) 470
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 6 cut(s) 111, 156, 332, 339, 436, 473
AluI AGCT 6 cut(s) 111, 156, 332, 339, 436, 473
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 2 cut(s) 131, 554
AlwI GGATC 1 cut(s) 575
AlwNI CAGNNNCTG 1 cut(s) 15
AoxI GGCC 2 cut(s) 115, 248
ApeKI GCWGC 4 cut(s) 137, 156, 264, 359
AspLEI GCGC 2 cut(s) 161, 163
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 422
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 4 cut(s) 124, 143, 276, 371
BccI CCATC 2 cut(s) 507, 542
BciT130I CCWGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 131, 554
BfaI CTAG 1 cut(s) 560
BisI GCNGC 4 cut(s) 138, 157, 265, 360
BlpI GCTNAGC 1 cut(s) 107
BlsI GCNGC 4 cut(s) 139, 158, 266, 361
Bme1390I CCNGG 1 cut(s) 381
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 381
BmrI ACTGGG 1 cut(s) 117
BmuI ACTGGG 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 211, 237
BsaHI GRCGYC 1 cut(s) 73
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 235
Bse1I ACTGG 1 cut(s) 123
Bse3DI GCAATG 1 cut(s) 246
BseBI CCWGG 1 cut(s) 381
BseGI GGATG 2 cut(s) 56, 499
BseLI CCNNNNNNNGG 2 cut(s) 89, 235
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 123
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 4 cut(s) 124, 143, 276, 371
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 117, 250
BsiHKAI GWGCWC 1 cut(s) 108
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 2 cut(s) 89, 235
BsmAI GTCTC 2 cut(s) 131, 554
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 2 cut(s) 95, 384
BsnI GGCC 2 cut(s) 117, 250
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 212, 567
Bsp1720I GCTNAGC 1 cut(s) 107
BspACI CCGC 1 cut(s) 135
BspANI GGCC 2 cut(s) 117, 250
BspCNI CTCAG 1 cut(s) 120
BspFNI CGCG 1 cut(s) 161
BspPI GGATC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 246
BsrI ACTGG 1 cut(s) 123
BssHII GCGCGC 1 cut(s) 159
BssMI GATC 2 cut(s) 212, 567
BssNI GRCGYC 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 274, 301
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 73
BstC8I GCNNGC 4 cut(s) 104, 142, 161, 544
BstDEI CTNAG 1 cut(s) 107
BstF5I GGATG 2 cut(s) 56, 499
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 2 cut(s) 161, 163
BstKTI GATC 2 cut(s) 215, 570
BstMAI GTCTC 2 cut(s) 131, 554
BstMBI GATC 2 cut(s) 212, 567
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 1 cut(s) 381
BstNSI RCATGY 2 cut(s) 366, 488
BstSCI CCNGG 1 cut(s) 379
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 4 cut(s) 124, 143, 276, 371
BsuRI GGCC 2 cut(s) 117, 250
BtsCI GGATG 2 cut(s) 56, 499
BtsIMutI CAGTG 3 cut(s) 270, 297, 407
Cac8I GCNNGC 4 cut(s) 104, 142, 161, 544
CaiI CAGNNNCTG 1 cut(s) 15
CfoI GCGC 2 cut(s) 161, 163
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 4 cut(s) 275, 302, 377, 416
CviAII CATG 2 cut(s) 363, 485
CviQI GTAC 4 cut(s) 275, 302, 377, 416
DdeI CTNAG 1 cut(s) 107
DpnI GATC 2 cut(s) 214, 569
DpnII GATC 2 cut(s) 212, 567
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 4
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 379
FaeI CATG 2 cut(s) 366, 488
FaiI YATR 7 cut(s) 294, 364, 374, 443, 486, 505, 507
FaqI GGGAC 1 cut(s) 497
FatI CATG 2 cut(s) 362, 484
FauNDI CATATG 1 cut(s) 505
Fnu4HI GCNGC 4 cut(s) 138, 157, 265, 360
FokI GGATG 2 cut(s) 43, 486
Fsp4HI GCNGC 4 cut(s) 138, 157, 265, 360
FspBI CTAG 1 cut(s) 560
GlaI GCGC 2 cut(s) 160, 162
GluI GCNGC 4 cut(s) 138, 157, 265, 360
HaeIII GGCC 2 cut(s) 117, 250
HhaI GCGC 2 cut(s) 161, 163
Hin1I GRCGYC 1 cut(s) 73
Hin1II CATG 2 cut(s) 366, 488
Hin6I GCGC 2 cut(s) 159, 161
HinP1I GCGC 2 cut(s) 159, 161
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 2 cut(s) 12, 425
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 5 cut(s) 23, 92, 212, 347, 430
Hpy188III TCNNGA 4 cut(s) 9, 190, 448, 571
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 3 cut(s) 75, 78, 92
HpyAV CCTTC 4 cut(s) 48, 52, 239, 401
HpyCH4III ACNGT 2 cut(s) 274, 301
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 2 cut(s) 144, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 1 cut(s) 73
Hsp92I GRCGYC 1 cut(s) 73
Hsp92II CATG 2 cut(s) 366, 488
HspAI GCGC 2 cut(s) 159, 161
Kzo9I GATC 2 cut(s) 212, 567
LmnI GCTCC 2 cut(s) 92, 433
LpnPI CCDG 8 cut(s) 22, 136, 166, 366, 393, 466, 528, 528
Lsp1109I GCAGC 4 cut(s) 124, 143, 276, 371
MaeI CTAG 1 cut(s) 560
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 1 cut(s) 295
MalI GATC 2 cut(s) 214, 569
MboI GATC 2 cut(s) 212, 567
MboII GAAGA 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 1 cut(s) 197
MlyI GAGTC 1 cut(s) 6
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 9 cut(s) 64, 70, 128, 174, 186, 195, 261, 406, 424
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 2 cut(s) 137, 156
MspR9I CCNGG 1 cut(s) 381
Mva1269I GAATGC 2 cut(s) 95, 384
MvaI CCWGG 1 cut(s) 381
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeI CATATG 1 cut(s) 505
NdeII GATC 2 cut(s) 212, 567
NlaIII CATG 2 cut(s) 366, 488
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 2 cut(s) 366, 488
PauI GCGCGC 1 cut(s) 159
PctI GAATGC 2 cut(s) 95, 384
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 4 cut(s) 139, 158, 266, 361
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
PsiI TTATAA 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspPI GGNCC 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 15
PteI GCGCGC 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 156
RsaI GTAC 4 cut(s) 276, 303, 378, 417
RsaNI GTAC 4 cut(s) 275, 302, 377, 416
RseI CAYNNNNRTG 1 cut(s) 504
SatI GCNGC 4 cut(s) 138, 157, 265, 360
Sau3AI GATC 2 cut(s) 212, 567
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 1 cut(s) 108
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 2 cut(s) 190, 252
SmoI CTYRAG 2 cut(s) 190, 252
Sse9I AATT 1 cut(s) 197
SsiI CCGC 1 cut(s) 135
SspMI CTAG 1 cut(s) 560
StyD4I CCNGG 1 cut(s) 379
TaaI ACNGT 2 cut(s) 274, 301
TaiI ACGT 1 cut(s) 76
TasI AATT 1 cut(s) 197
TfiI GAWTC 1 cut(s) 425
TscAI CASTG 3 cut(s) 277, 304, 407
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 4 cut(s) 137, 156, 264, 359
Tsp45I GTSAC 1 cut(s) 295
TspRI CASTG 3 cut(s) 277, 304, 407
XceI RCATGY 2 cut(s) 366, 488
XspI CTAG 1 cut(s) 560
ZraI GACGTC 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.