MD15G1382000.v1.1

Protein CutA, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006754
Physical Location & Seq
Forward (+)
47100354 .. 47102820
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1382000.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCTTCTACACTCTTCTGCAGACCCAGAGCCCTTCGAACCATCTCCTCCTCCGCCACGTTACGACGCCGTCTTCCCCTGGTGGGAGCATTCTGCGTTGTCACCTTGGGCATCTCCAACCTCCTTCCCACTCTCCCCTTCTCCGCCTTCAGAACCGGCACTGCTCTCTCTCTCCCTTTTGCCCCTCTCTTGAGATCAAGGTTTGGCAGCCAATCAGCAGCCGTTCATTCTGTGAGGATGGAAGGGAGTAGCAGTGCCACTGTTCCAAGTATTGTTGTTTATGTGACTGTGCCCAGCAGAGAAGTAGGAAAGAAGTTGGCTGAAAGCTTAGTCAGAGAACAACTTGCAGCCTGTGTTAACCTAGTGCCAGGCATTGAGTCCGTCTATCAGTGGAAAGGAGAGGTCCAGACAGATTCTGAAGAACTTCTCATAATCAAGACAAGGCAATCCCTTTTTGAAGGTCTGACAGCGCATGTCAAGGCAAACCATCCATACGATGTTCCAGAAGTTATCGCCTTACCCATCAACGCAGGCAGTCTCCCGTACTTAGAATGGATCAAAAACAGCACAAGGGACTGA

Protein Analysis

193

Amino Acids

20.88

Weight (kDa)

9.55

Isoelectric Point (pI)

53.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CutA1 PF03091 91 - 188 8.3e-38 CutA1 divalent ion tolerance protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 54, 144
AclWI GGATC 1 cut(s) 563
AcuI CTGAAG 2 cut(s) 133, 438
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 1 cut(s) 458
AjnI CCWGG 2 cut(s) 78, 367
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 1 cut(s) 563
AlwNI CAGNNNCTG 1 cut(s) 416
ApeKI GCWGC 3 cut(s) 207, 218, 347
Asp700I GAANNNNTTC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 403
BaeGI GKGCMC 1 cut(s) 294
BanII GRGCYC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 219, 230, 359
BccI CCATC 4 cut(s) 50, 232, 495, 530
BceAI ACGGC 2 cut(s) 54, 206
BciT130I CCWGG 2 cut(s) 80, 369
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 3 cut(s) 208, 219, 348
BlsI GCNGC 3 cut(s) 209, 220, 349
Bme1390I CCNGG 2 cut(s) 80, 369
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmrFI CCNGG 2 cut(s) 80, 369
BmsI GCATC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 78, 105
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse118I RCCGGY 1 cut(s) 155
BseBI CCWGG 2 cut(s) 80, 369
BseDI CCNNGG 2 cut(s) 78, 105
BseGI GGATG 2 cut(s) 243, 487
BseLI CCNNNNNNNGG 1 cut(s) 83
BseRI GAGGAG 2 cut(s) 37, 40
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 3 cut(s) 219, 230, 359
BseYI CCCAGC 1 cut(s) 293
BsiSI CCGG 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 34, 294
Bsp143I GATC 2 cut(s) 194, 555
BspACI CCGC 2 cut(s) 54, 144
BspMAI CTGCAG 1 cut(s) 23
BspPI GGATC 1 cut(s) 563
BspT104I TTCGAA 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 78, 105
BssMI GATC 2 cut(s) 194, 555
BssNI GRCGYC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 80, 369
Bst4CI ACNGT 2 cut(s) 262, 289
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 67
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 2 cut(s) 328, 547
BstF5I GGATG 2 cut(s) 243, 487
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 2 cut(s) 197, 558
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 2 cut(s) 194, 555
BstNI CCWGG 2 cut(s) 80, 369
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 78, 367
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 219, 230, 359
BstV2I GAAGAC 1 cut(s) 65
BtsCI GGATG 2 cut(s) 243, 487
BtsI GCAGTG 2 cut(s) 159, 259
BtsIMutI CAGTG 4 cut(s) 159, 258, 259, 395
Cac8I GCNNGC 1 cut(s) 532
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 403
CseI GACGC 1 cut(s) 75
Csp6I GTAC 1 cut(s) 544
CviAII CATG 1 cut(s) 473
CviJI RGCY 7 cut(s) 5, 32, 210, 221, 320, 327, 350
CviKI_1 RGCY 7 cut(s) 5, 32, 210, 221, 320, 327, 350
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 2 cut(s) 328, 547
DpnI GATC 2 cut(s) 196, 557
DpnII GATC 2 cut(s) 194, 555
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 2 cut(s) 43, 133
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 2 cut(s) 133, 438
EcoRII CCWGG 2 cut(s) 78, 367
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 1 cut(s) 476
FaiI YATR 4 cut(s) 282, 431, 474, 493
FatI CATG 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 208, 219, 348
FokI GGATG 2 cut(s) 250, 474
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 3 cut(s) 208, 219, 348
FspBI CTAG 1 cut(s) 362
GlaI GCGC 1 cut(s) 471
GluI GCNGC 3 cut(s) 208, 219, 348
GsaI CCCAGC 1 cut(s) 297
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 75
HhaI GCGC 1 cut(s) 472
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 1 cut(s) 476
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 2 cut(s) 377, 413
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 4 cut(s) 152, 335, 418, 465
Hpy188III TCNNGA 4 cut(s) 190, 406, 436, 503
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 6 cut(s) 44, 134, 148, 157, 236, 452
HpyCH4III ACNGT 2 cut(s) 262, 289
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 21, 347
HpyF3I CTNAG 2 cut(s) 328, 547
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 1 cut(s) 476
HspAI GCGC 1 cut(s) 470
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 194, 555
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 3 cut(s) 219, 230, 359
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 3 cut(s) 60, 100, 283
MalI GATC 2 cut(s) 196, 557
MboI GATC 2 cut(s) 194, 555
MboII GAAGA 3 cut(s) 7, 65, 431
MhlI GDGCHC 2 cut(s) 34, 294
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 6 cut(s) 58, 61, 131, 195, 228, 394
MroXI GAANNNNTTC 1 cut(s) 423
MseI TTAA 1 cut(s) 357
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 80, 369
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 2 cut(s) 80, 369
NdeII GATC 2 cut(s) 194, 555
NlaIII CATG 1 cut(s) 476
NmuCI GTSAC 2 cut(s) 100, 283
NspI RCATGY 1 cut(s) 476
NspV TTCGAA 1 cut(s) 37
PctI GAATGC 1 cut(s) 89
PdmI GAANNNNTTC 1 cut(s) 423
PfeI GAWTC 1 cut(s) 413
PflFI GACNNNGTC 1 cut(s) 69
PkrI GCNGC 3 cut(s) 209, 220, 349
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 78, 367
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 2 cut(s) 78, 367
PspPI GGNCC 1 cut(s) 403
PstI CTGCAG 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 416
PsyI GACNNNGTC 1 cut(s) 69
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 3 cut(s) 208, 219, 348
Sau3AI GATC 2 cut(s) 194, 555
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 80, 369
SduI GDGCHC 2 cut(s) 34, 294
SetI ASST 8 cut(s) 62, 107, 123, 203, 329, 363, 405, 463
SfaNI GCATC 1 cut(s) 120
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 37
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
SsiI CCGC 2 cut(s) 54, 144
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 78, 367
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 262, 289
TaiI ACGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 37
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 4 cut(s) 166, 259, 265, 395
TseFI GTSAC 2 cut(s) 100, 283
TseI GCWGC 3 cut(s) 207, 218, 347
Tsp45I GTSAC 2 cut(s) 100, 283
TspDTI ATGAA 1 cut(s) 215
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 4 cut(s) 166, 259, 265, 395
Tth111I GACNNNGTC 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 403
XceI RCATGY 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 423
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.