AT2G35360

Uncharacterized conserved domain (SAYSvFN)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010199
Physical Location & Seq
Reverse (-)
14889048 .. 14891004
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35360.3

Sequence Viewer

Length: 678 bp
ATGATGAATTCAACCGAAGAGCTGAGCTCAGATTCAACCAACAATGGCACCGTCGAGATCACGTACAAAACCATCGGCCCAGCTCGGCCGTCTCAAATTCGAGTGGCGTCTCATATCAAAATTCGTGATTTGAGAAATGCAATTGCGGAAAAGGGTAAATTTCCCGTTTCAACTTTAAGGATGATTCTTCGTGGAAAGGCATTGCAAGACGAAGAAGATGGAGATGATTTATACGTTACGCTCAAGGACCAAGATTCCTTTATTGTTGCTGTAATACCAAATCCACCAGCTGGAGTTGAGTCATTTGATGATGATGATGATGATGATTTGAAGTTTAAACTCCCACCGTCAGCAAGCCGGTGGAAGAGGAAGTTATACTACTTTCTGCGTAACAAGTTGAAGCTGCCAGATATCATTCTTATGGGACTTTTCTCTTTAAGTCTAAAGATGTGGGTTATTATCACCTTGTGGTTTATCTTGGCGCCTATTGCACATCGGTGGGATCTGGGCCCTATATTTATACTAGGGACTGGATTTTCAATCATCTTGCTCAATCTTGGTAAACGACAGCCTGGTGATGTTAGTGCATATTCCATATTCAACGAGGATTTTAGAGAGCTTCCAGGTACATATAATGCAGATCGTATAGACCGGGACATACGAGCAGGCCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAYSvFN ubiquitin

Protein Analysis

225

Amino Acids

25.52

Weight (kDa)

5.77

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35360 AT2G35360 AT2G35360
fragaria_vesca FvH4_5g21620 FvH4_5g21620 FvH4_5g21620
malus_domestica MD06G1031100.v1.1
prunus_persica Prupe.5G041200_v2.0.a1 Prupe.5G041200_v2.0.a1
pyrus_communis pycom06g02590
rosa_chinensis RchiOBHm_Chr7g0209081
rosa_laevigata RLG00000003169
rosa_multiflora Rmu_co8263433.1_g000001
rosa_roxburghii Rroxscaffold_3G00249770
rosa_rugosa Rorug07G0108800 Rorug07G0108900
rosa_samantha Rh7AG243300 Rh7BG238300 Rh7CG258500 Rh7DG250300
rosa_wichuraiana Rw7G020620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 47, 481
AccB7I CCANNNNNTGG 1 cut(s) 290
AciI CCGC 1 cut(s) 146
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 4 cut(s) 7, 96, 120, 158
AcyI GRCGYC 2 cut(s) 107, 482
AdeI CACNNNGTG 1 cut(s) 468
AfaI GTAC 2 cut(s) 65, 628
AfiI CCNNNNNNNGG 1 cut(s) 290
AgsI TTSAA 7 cut(s) 12, 36, 171, 331, 400, 540, 601
AjnI CCWGG 2 cut(s) 571, 622
AleI CACNNNNGTG 1 cut(s) 496
AluBI AGCT 6 cut(s) 22, 27, 83, 290, 403, 619
AluI AGCT 6 cut(s) 22, 27, 83, 290, 403, 619
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 2 cut(s) 96, 114
AlwI GGATC 1 cut(s) 510
AoxI GGCC 4 cut(s) 76, 86, 508, 667
ApaI GGGCCC 1 cut(s) 512
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 4 cut(s) 7, 96, 120, 158
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 4 cut(s) 77, 247, 508, 509
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 2 cut(s) 454, 587
AvaII GGWCC 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 512
BanI GGYRCC 2 cut(s) 47, 481
BanII GRGCYC 2 cut(s) 29, 512
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 390
BccI CCATC 2 cut(s) 80, 212
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 573, 624
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 2 cut(s) 96, 114
BfaI CTAG 1 cut(s) 524
BfoI RGCGCY 1 cut(s) 485
BisI GCNGC 1 cut(s) 404
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 405
Bme1390I CCNGG 3 cut(s) 573, 624, 653
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 4 cut(s) 77, 247, 508, 509
BmiI GGNNCC 3 cut(s) 49, 483, 510
BmrFI CCNGG 3 cut(s) 573, 624, 653
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 653
BsaAI YACGTR 1 cut(s) 63
BsaHI GRCGYC 2 cut(s) 107, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 535
Bse3DI GCAATG 1 cut(s) 200
BseBI CCWGG 2 cut(s) 573, 624
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMI GCAATG 1 cut(s) 200
BseMII CTCAG 2 cut(s) 14, 42
BseNI ACTGG 1 cut(s) 535
BseSI GKGCMC 1 cut(s) 512
BseX3I CGGCCG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 390
BseYI CCCAGC 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 4 cut(s) 78, 88, 510, 669
BshNI GGYRCC 2 cut(s) 47, 481
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 2 cut(s) 358, 652
BslFI GGGAC 3 cut(s) 438, 541, 668
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 2 cut(s) 96, 114
BsmBI CGTCTC 2 cut(s) 96, 114
BsmFI GGGAC 3 cut(s) 438, 541, 668
BsnI GGCC 4 cut(s) 78, 88, 510, 669
Bsp120I GGGCCC 1 cut(s) 508
Bsp1286I GDGCHC 2 cut(s) 29, 512
Bsp143I GATC 3 cut(s) 57, 502, 640
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 1 cut(s) 146
BspANI GGCC 4 cut(s) 78, 88, 510, 669
BspCNI CTCAG 2 cut(s) 15, 41
BspLI GGNNCC 3 cut(s) 49, 483, 510
BspPI GGATC 1 cut(s) 510
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 47, 481
BsrDI GCAATG 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 357
BsrI ACTGG 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 357
BssMI GATC 3 cut(s) 57, 502, 640
BssNI GRCGYC 2 cut(s) 107, 482
Bst2UI CCWGG 2 cut(s) 573, 624
Bst4CI ACNGT 2 cut(s) 52, 348
Bst6I CTCTTC 2 cut(s) 12, 359
BstACI GRCGYC 2 cut(s) 107, 482
BstBAI YACGTR 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 355, 667
BstDEI CTNAG 2 cut(s) 23, 28
BstF5I GGATG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 3 cut(s) 60, 505, 643
BstMAI GTCTC 2 cut(s) 96, 114
BstMBI GATC 3 cut(s) 57, 502, 640
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 2 cut(s) 573, 624
BstSCI CCNGG 3 cut(s) 571, 622, 651
BstSLI GKGCMC 1 cut(s) 512
BstV1I GCAGC 1 cut(s) 390
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BstZI CGGCCG 1 cut(s) 86
BsuRI GGCC 4 cut(s) 78, 88, 510, 669
BtsCI GGATG 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 355, 667
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 357
Cfr13I GGNCC 4 cut(s) 77, 247, 508, 509
CseI GACGC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 64, 627
CviQI GTAC 2 cut(s) 64, 627
DdeI CTNAG 2 cut(s) 23, 28
DinI GGCGCC 1 cut(s) 483
DpnI GATC 3 cut(s) 59, 504, 642
DpnII GATC 3 cut(s) 57, 502, 640
DraI TTTAAA 1 cut(s) 337
DraIII CACNNNGTG 1 cut(s) 468
EaeI YGGCCR 1 cut(s) 86
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 2 cut(s) 12, 359
EarI CTCTTC 2 cut(s) 12, 359
Ecl136II GAGCTC 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 86
Eco24I GRGCYC 2 cut(s) 29, 512
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 247
Eco52I CGGCCG 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 571, 622
EcoRV GATATC 1 cut(s) 412
EcoT38I GRGCYC 2 cut(s) 29, 512
EgeI GGCGCC 1 cut(s) 483
EheI GGCGCC 1 cut(s) 483
Esp3I CGTCTC 2 cut(s) 96, 114
FaqI GGGAC 3 cut(s) 438, 541, 668
Fnu4HI GCNGC 1 cut(s) 404
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 2 cut(s) 29, 512
Fsp4HI GCNGC 1 cut(s) 404
FspBI CTAG 1 cut(s) 524
GlaI GCGC 1 cut(s) 483
GluI GCNGC 1 cut(s) 404
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 312
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 4 cut(s) 78, 88, 510, 669
HapII CCGG 2 cut(s) 358, 652
HgaI GACGC 1 cut(s) 96
HhaI GCGC 1 cut(s) 484
Hin1I GRCGYC 2 cut(s) 107, 482
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HinfI GANTC 4 cut(s) 32, 184, 254, 299
HpaII CCGG 2 cut(s) 358, 652
HphI GGTGA 2 cut(s) 454, 587
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 1 cut(s) 31
Hpy188III TCNNGA 2 cut(s) 55, 125
Hpy8I GTNNAC 1 cut(s) 563
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 52, 348
HpyCH4IV ACGT 2 cut(s) 62, 234
HpyCH4V TGCA 5 cut(s) 140, 205, 491, 587, 638
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 2 cut(s) 23, 28
HpySE526I ACGT 2 cut(s) 62, 234
Hsp92I GRCGYC 2 cut(s) 107, 482
HspAI GCGC 1 cut(s) 482
KasI GGCGCC 1 cut(s) 481
Kzo9I GATC 3 cut(s) 57, 502, 640
LguI GCTCTTC 1 cut(s) 12
Lsp1109I GCAGC 1 cut(s) 390
MaeI CTAG 1 cut(s) 524
MaeII ACGT 2 cut(s) 62, 234
MaeIII GTNAC 2 cut(s) 235, 389
MalI GATC 3 cut(s) 59, 504, 642
MboI GATC 3 cut(s) 57, 502, 640
MboII GAAGA 5 cut(s) 29, 179, 224, 227, 376
MfeI CAATTG 1 cut(s) 141
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 2 cut(s) 29, 512
MluCI AATT 5 cut(s) 7, 96, 120, 141, 158
Mly113I GGCGCC 1 cut(s) 482
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 2 cut(s) 360, 598
MseI TTAA 3 cut(s) 176, 336, 437
MslI CAYNNNNRTG 2 cut(s) 419, 496
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 358, 652
MspR9I CCNGG 3 cut(s) 573, 624, 653
MssI GTTTAAAC 1 cut(s) 337
MunI CAATTG 1 cut(s) 141
MvaI CCWGG 2 cut(s) 573, 624
MwoI GCNNNNNNNGC 1 cut(s) 488
NarI GGCGCC 1 cut(s) 482
NciI CCSGG 1 cut(s) 653
NdeII GATC 3 cut(s) 57, 502, 640
NlaIV GGNNCC 3 cut(s) 49, 483, 510
NmeAIII GCCGAG 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 496
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 32, 184, 254
PflMI CCANNNNNTGG 1 cut(s) 290
PkrI GCNGC 1 cut(s) 405
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 1 cut(s) 485
PmeI GTTTAAAC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 307
Ppu21I YACGTR 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 571, 622
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 2 cut(s) 571, 622
PspN4I GGNNCC 3 cut(s) 49, 483, 510
PspOMI GGGCCC 1 cut(s) 508
PspPI GGNCC 4 cut(s) 77, 247, 508, 509
PsuI RGATCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 65, 628
RsaNI GTAC 2 cut(s) 64, 627
RseI CAYNNNNRTG 2 cut(s) 419, 496
SacI GAGCTC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 176, 336, 437
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 3 cut(s) 57, 502, 640
Sau96I GGNCC 4 cut(s) 77, 247, 508, 509
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 573, 624, 653
SduI GDGCHC 2 cut(s) 29, 512
SfoI GGCGCC 1 cut(s) 483
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 419, 496
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 5 cut(s) 7, 96, 120, 141, 158
SsiI CCGC 1 cut(s) 146
SspDI GGCGCC 1 cut(s) 481
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 571, 622, 651
TaaI ACNGT 2 cut(s) 52, 348
TaiI ACGT 2 cut(s) 65, 237
TaqI TCGA 2 cut(s) 54, 100
TasI AATT 5 cut(s) 7, 96, 120, 141, 158
TfiI GAWTC 3 cut(s) 32, 184, 254
Tru1I TTAA 3 cut(s) 176, 336, 437
Tru9I TTAA 3 cut(s) 176, 336, 437
TseI GCWGC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 20
Van91I CCANNNNNTGG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 247
XapI RAATTY 4 cut(s) 7, 96, 120, 158
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.