FvH4_5g21620

Uncharacterized conserved domain (SAYSvFN)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010199
Physical Location & Seq
Forward (+)
13044871 .. 13051281
6411 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21620.t3

Sequence Viewer

Length: 702 bp
ATGGCTGAGATTGTAGAGCAACACATGAATCCCAGGTGCAATGGCGACCCTAGATTTCTTAACAAGGTTGACATCCTTGTACGGACCATCGGCCCATCTCGCCCTTCTCGTCTCCAAGTCTCCTCTTCAATCACTGTGCTTGACTTAAGAAAGTTGATTGCTGAAAATTATCAGTTACCATTAGAAAAGTTGAAGCTTATTATGCGAGGCAATGTGTTGCATGACAGCAAAAGTGGGCAAGATGTGAATCTCCATCTCAAGGATGGCGATTCTCTTATTGTTGCTGTCAAACCAAAGCCACCAGCTAAGCATCTTCGGGATGGATTTGATGATGATGACGATGAAGATGATCTGAAGTTTCAGCTTCCTCAGTCTTCAAGCCGGTGGAAGAGGAGAGTTTATGCTGTTCTACATGATAAGTTGAAGCTTCCTGATATTCTTTTGATGGCCATTTTCTCTCTCAGTTTGAAGATGTGGGTAATTATCATTATGTGGTTTATATTGGCACCTGTGGCCCACAGATGGGATGTTGGCCCTTTATATATACTAGGCACCGGTTTTGGTATCATTCTTCTGAATCTAGGACAGCGGAAGGCTGGCGATGTCAGTGCATATTCGATTTTCAATGAAGATTTTAGAGAGCTTCCAGGGACCCTTAATGCAGAACGTCTTGATGGGGACATACGAACAGGTCAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAYSvFN ubiquitin

Protein Analysis

234

Amino Acids

26.43

Weight (kDa)

7.86

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 26 - 98 1.9e-07 Ubiquitin family
SAYSvFN PF10260 162 - 228 1.2e-24 Uncharacterized conserved domain (SAYSvFN)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35360 AT2G35360 AT2G35360
fragaria_vesca FvH4_5g21620 FvH4_5g21620 FvH4_5g21620
malus_domestica MD06G1031100.v1.1
prunus_persica Prupe.5G041200_v2.0.a1 Prupe.5G041200_v2.0.a1
pyrus_communis pycom06g02590
rosa_chinensis RchiOBHm_Chr7g0209081
rosa_laevigata RLG00000003169
rosa_multiflora Rmu_co8263433.1_g000001
rosa_roxburghii Rroxscaffold_3G00249770
rosa_rugosa Rorug07G0108800 Rorug07G0108900
rosa_samantha Rh7AG243300 Rh7BG238300 Rh7CG258500 Rh7DG250300
rosa_wichuraiana Rw7G020620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 505, 551
AciI CCGC 1 cut(s) 589
AcoI YGGCCR 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 3 cut(s) 259, 522, 523
AflII CTTAAG 1 cut(s) 145
AgeI ACCGGT 1 cut(s) 554
AgsI TTSAA 6 cut(s) 129, 193, 378, 424, 469, 625
AjnI CCWGG 2 cut(s) 32, 646
AluBI AGCT 5 cut(s) 196, 305, 364, 427, 643
AluI AGCT 5 cut(s) 196, 305, 364, 427, 643
Alw26I GTCTC 2 cut(s) 116, 124
AoxI GGCC 4 cut(s) 91, 447, 513, 532
AsiGI ACCGGT 1 cut(s) 554
AspS9I GGNCC 5 cut(s) 84, 92, 514, 533, 651
AvaII GGWCC 2 cut(s) 84, 651
BalI TGGCCA 1 cut(s) 449
BanI GGYRCC 2 cut(s) 505, 551
BbsI GAAGAC 1 cut(s) 366
BccI CCATC 8 cut(s) 95, 103, 257, 261, 314, 439, 516, 668
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 2 cut(s) 34, 648
BcoDI GTCTC 2 cut(s) 116, 124
BfaI CTAG 3 cut(s) 51, 548, 581
BfrI CTTAAG 1 cut(s) 145
BlpI GCTNAGC 1 cut(s) 306
Bme1390I CCNGG 2 cut(s) 34, 648
Bme18I GGWCC 2 cut(s) 84, 651
BmgT120I GGNCC 5 cut(s) 84, 92, 514, 533, 651
BmiI GGNNCC 4 cut(s) 507, 553, 652, 653
BmrFI CCNGG 2 cut(s) 34, 648
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 32, 647
BsaWI WCCGGW 1 cut(s) 554
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 522, 523
Bse118I RCCGGY 2 cut(s) 381, 554
Bse3DI GCAATG 2 cut(s) 46, 217
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 2 cut(s) 34, 648
BseDI CCNNGG 2 cut(s) 32, 647
BseGI GGATG 4 cut(s) 72, 268, 325, 532
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 3 cut(s) 259, 522, 523
BseMI GCAATG 2 cut(s) 46, 217
BseMII CTCAG 2 cut(s) 383, 475
BseRI GAGGAG 2 cut(s) 112, 406
BshFI GGCC 4 cut(s) 93, 449, 515, 534
BshNI GGYRCC 2 cut(s) 505, 551
BshTI ACCGGT 1 cut(s) 554
BsiSI CCGG 2 cut(s) 382, 555
BslFI GGGAC 2 cut(s) 664, 692
BslI CCNNNNNNNGG 3 cut(s) 259, 522, 523
BsmAI GTCTC 2 cut(s) 116, 124
BsmBI CGTCTC 1 cut(s) 116
BsmFI GGGAC 2 cut(s) 664, 692
BsnI GGCC 4 cut(s) 93, 449, 515, 534
Bsp143I GATC 1 cut(s) 349
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 1 cut(s) 589
BspANI GGCC 4 cut(s) 93, 449, 515, 534
BspCNI CTCAG 2 cut(s) 382, 474
BspLI GGNNCC 4 cut(s) 507, 553, 652, 653
BspT107I GGYRCC 2 cut(s) 505, 551
BspTI CTTAAG 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 46, 217
BsrFI RCCGGY 2 cut(s) 381, 554
BssAI RCCGGY 2 cut(s) 381, 554
BssECI CCNNGG 2 cut(s) 32, 647
BssMI GATC 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 34, 648
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 2 cut(s) 130, 383
BstAFI CTTAAG 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 598
BstDEI CTNAG 4 cut(s) 6, 306, 369, 461
BstF5I GGATG 4 cut(s) 72, 268, 325, 532
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 2 cut(s) 116, 124
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 99, 202, 512
BstNI CCWGG 2 cut(s) 34, 648
BstSCI CCNGG 2 cut(s) 32, 646
BstV2I GAAGAC 1 cut(s) 366
BsuRI GGCC 4 cut(s) 93, 449, 515, 534
BtgZI GCGATG 1 cut(s) 615
BtsCI GGATG 4 cut(s) 72, 268, 325, 532
BtsIMutI CAGTG 2 cut(s) 132, 613
Cac8I GCNNGC 1 cut(s) 598
Cfr10I RCCGGY 2 cut(s) 381, 554
Cfr13I GGNCC 5 cut(s) 84, 92, 514, 533, 651
Csp6I GTAC 1 cut(s) 80
CspAI ACCGGT 1 cut(s) 554
CviAII CATG 3 cut(s) 25, 221, 413
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 4 cut(s) 6, 306, 369, 461
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 130, 383
EarI CTCTTC 2 cut(s) 130, 383
Eco47I GGWCC 2 cut(s) 84, 651
Eco57I CTGAAG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 651
EcoRII CCWGG 2 cut(s) 32, 646
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 3 cut(s) 28, 224, 416
FaqI GGGAC 2 cut(s) 664, 692
FatI CATG 3 cut(s) 24, 220, 412
FokI GGATG 4 cut(s) 59, 275, 332, 539
FspBI CTAG 3 cut(s) 51, 548, 581
HaeIII GGCC 4 cut(s) 93, 449, 515, 534
HapII CCGG 2 cut(s) 382, 555
Hin1II CATG 3 cut(s) 28, 224, 416
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 2 cut(s) 194, 425
HinfI GANTC 4 cut(s) 28, 247, 269, 577
HpaII CCGG 2 cut(s) 382, 555
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 3 cut(s) 354, 576, 696
Hpy188III TCNNGA 3 cut(s) 317, 431, 671
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 2 cut(s) 114, 586
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 1 cut(s) 667
HpyCH4V TGCA 4 cut(s) 39, 220, 611, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 202, 512
HpyF3I CTNAG 4 cut(s) 6, 306, 369, 461
HpySE526I ACGT 1 cut(s) 667
Hsp92II CATG 3 cut(s) 28, 224, 416
KflI GGGWCCC 1 cut(s) 651
Kzo9I GATC 1 cut(s) 349
LweI GCATC 1 cut(s) 319
MaeI CTAG 3 cut(s) 51, 548, 581
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 1 cut(s) 174
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 8 cut(s) 117, 305, 356, 366, 400, 481, 563, 641
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 2 cut(s) 166, 480
MluNI TGGCCA 1 cut(s) 449
MnlI CCTC 4 cut(s) 133, 200, 378, 384
Mox20I TGGCCA 1 cut(s) 449
MscI TGGCCA 1 cut(s) 449
MseI TTAA 3 cut(s) 60, 146, 657
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 589
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 2 cut(s) 382, 555
MspR9I CCNGG 2 cut(s) 34, 648
MvaI CCWGG 2 cut(s) 34, 648
MwoI GCNNNNNNNGC 3 cut(s) 99, 202, 512
NdeII GATC 1 cut(s) 349
NlaIII CATG 3 cut(s) 28, 224, 416
NlaIV GGNNCC 4 cut(s) 507, 553, 652, 653
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PfeI GAWTC 4 cut(s) 28, 247, 269, 577
PinAI ACCGGT 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 651
Psp6I CCWGG 2 cut(s) 32, 646
PspGI CCWGG 2 cut(s) 32, 646
PspN4I GGNNCC 4 cut(s) 507, 553, 652, 653
PspPI GGNCC 5 cut(s) 84, 92, 514, 533, 651
PspPPI RGGWCCY 1 cut(s) 651
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 60, 146, 657
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 5 cut(s) 84, 92, 514, 533, 651
ScrFI CCNGG 2 cut(s) 34, 648
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 2 cut(s) 84, 651
SmlI CTYRAG 2 cut(s) 145, 257
SmoI CTYRAG 2 cut(s) 145, 257
Sse9I AATT 2 cut(s) 166, 480
SsiI CCGC 1 cut(s) 589
SspMI CTAG 3 cut(s) 51, 548, 581
StyD4I CCNGG 2 cut(s) 32, 646
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 1 cut(s) 670
TaqI TCGA 1 cut(s) 617
TasI AATT 2 cut(s) 166, 480
TfiI GAWTC 4 cut(s) 28, 247, 269, 577
Tru1I TTAA 3 cut(s) 60, 146, 657
Tru9I TTAA 3 cut(s) 60, 146, 657
TscAI CASTG 2 cut(s) 139, 613
TspDTI ATGAA 3 cut(s) 41, 357, 642
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 139, 613
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 84, 651
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XspI CTAG 3 cut(s) 51, 548, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.