Rroxscaffold_3G00249770

Uncharacterized conserved domain (SAYSvFN)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010199
Physical Location & Seq
Reverse (-)
43549662 .. 43556503
6842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00249770.1

Sequence Viewer

Length: 708 bp
ATGGCTGAGATTGTAGAGGAAGAGAAGCTGATACCAATCAATCACAGAAACAATGGCGACAAGCAAGTTGGGATCGTTGTACGGACCATTGGCCCAGCTCCCCCTTCTCGTCTTCAGCTCCCCTCTTCAATCAGAGTGCTTGACTTGAGAAAATTGATAGCTGAAAAGTATCAGTTACCATTAGAGAAGTTGAGGCTTATTATGCAAGGCAATGTCTTGCATGACAGCAAAAGTGGGCAAGATGTGAATCTCCAACTCAAGGATGGCGATTCTCTTATTGTTGCTGTCAAACCAAAGCCACCTTCTAAGCATCTTCGGGATGGACTTGACGAGGACGACGAGGACGACGAAGATGATCTGAAGTTTCAGCTTCCCCAATCATCAAGCCGGTGGAAGAGGAGAGTATATGCTGTTCTACATGATAAGTTGAAGCTTCCTGATATCCTTTTGATGGCCATTTTCTCTCTCAGTTTGAAGATGTGGGTAATTATCATTATGTGGTTTATATTGGCACCTGTGGCCCACAGATGGGATCTTGGCCCTTTATATATACTTGGCACTGGTTTTGGTATCATTCTTCTGAATCTAGGACAGCGGAAGCCTGGCGATGTCAGTGCATATTCGATTTTCAATGAAGATTTTAGAGAGCTTCCAGGGACCCTTAATGCAGAACGTCTTGATGGGGACATACGGACAGGTCAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAYSvFN ubiquitin

Protein Analysis

235

Amino Acids

26.62

Weight (kDa)

6.23

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 39 - 98 3e-06 Ubiquitin family
SAYSvFN PF10260 164 - 230 8.8e-25 Uncharacterized conserved domain (SAYSvFN)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35360 AT2G35360 AT2G35360
fragaria_vesca FvH4_5g21620 FvH4_5g21620 FvH4_5g21620
malus_domestica MD06G1031100.v1.1
prunus_persica Prupe.5G041200_v2.0.a1 Prupe.5G041200_v2.0.a1
pyrus_communis pycom06g02590
rosa_chinensis RchiOBHm_Chr7g0209081
rosa_laevigata RLG00000003169
rosa_multiflora Rmu_co8263433.1_g000001
rosa_roxburghii Rroxscaffold_3G00249770
rosa_rugosa Rorug07G0108800 Rorug07G0108900
rosa_samantha Rh7AG243300 Rh7BG238300 Rh7CG258500 Rh7DG250300
rosa_wichuraiana Rw7G020620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 511
AciI CCGC 1 cut(s) 595
AclWI GGATC 2 cut(s) 80, 540
AcoI YGGCCR 1 cut(s) 453
AcuI CTGAAG 2 cut(s) 98, 380
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 4 cut(s) 259, 451, 528, 529
AgsI TTSAA 4 cut(s) 129, 430, 475, 631
AjnI CCWGG 2 cut(s) 601, 652
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AluBI AGCT 7 cut(s) 28, 98, 118, 161, 370, 433, 649
AluI AGCT 7 cut(s) 28, 98, 118, 161, 370, 433, 649
AlwI GGATC 2 cut(s) 80, 540
AoxI GGCC 4 cut(s) 91, 453, 519, 538
AspS9I GGNCC 5 cut(s) 84, 92, 520, 539, 657
AvaII GGWCC 2 cut(s) 84, 657
BalI TGGCCA 1 cut(s) 455
BanI GGYRCC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 104
BccI CCATC 5 cut(s) 257, 314, 445, 522, 674
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 2 cut(s) 603, 654
BfaI CTAG 1 cut(s) 587
Bme1390I CCNGG 2 cut(s) 603, 654
Bme18I GGWCC 2 cut(s) 84, 657
BmgT120I GGNCC 5 cut(s) 84, 92, 520, 539, 657
BmiI GGNNCC 3 cut(s) 513, 658, 659
BmrFI CCNGG 2 cut(s) 603, 654
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 166, 242
BsaBI GATNNNNATC 2 cut(s) 35, 246
BsaJI CCNNGG 1 cut(s) 653
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 451, 528, 529
Bse118I RCCGGY 1 cut(s) 387
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 1 cut(s) 217
Bse8I GATNNNNATC 2 cut(s) 35, 246
BseBI CCWGG 2 cut(s) 603, 654
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 2 cut(s) 268, 325
BseJI GATNNNNATC 2 cut(s) 35, 246
BseLI CCNNNNNNNGG 4 cut(s) 259, 451, 528, 529
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 481
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 1 cut(s) 412
BseYI CCCAGC 1 cut(s) 94
BshFI GGCC 4 cut(s) 93, 455, 521, 540
BshNI GGYRCC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 388
BslFI GGGAC 2 cut(s) 670, 698
BslI CCNNNNNNNGG 4 cut(s) 259, 451, 528, 529
BsmFI GGGAC 2 cut(s) 670, 698
BsnI GGCC 4 cut(s) 93, 455, 521, 540
Bsp143I GATC 3 cut(s) 72, 355, 532
BspACI CCGC 1 cut(s) 595
BspANI GGCC 4 cut(s) 93, 455, 521, 540
BspCNI CTCAG 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 513, 658, 659
BspPI GGATC 2 cut(s) 80, 540
BspT107I GGYRCC 1 cut(s) 511
BsrDI GCAATG 1 cut(s) 217
BsrFI RCCGGY 1 cut(s) 387
BsrI ACTGG 1 cut(s) 565
BssAI RCCGGY 1 cut(s) 387
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 3 cut(s) 72, 355, 532
Bst2UI CCWGG 2 cut(s) 603, 654
Bst6I CTCTTC 3 cut(s) 15, 130, 389
BstDEI CTNAG 3 cut(s) 6, 306, 467
BstF5I GGATG 2 cut(s) 268, 325
BstKTI GATC 3 cut(s) 75, 358, 535
BstMBI GATC 3 cut(s) 72, 355, 532
BstMWI GCNNNNNNNGC 2 cut(s) 202, 518
BstNI CCWGG 2 cut(s) 603, 654
BstSCI CCNGG 2 cut(s) 601, 652
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuRI GGCC 4 cut(s) 93, 455, 521, 540
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 2 cut(s) 268, 325
BtsIMutI CAGTG 2 cut(s) 558, 619
Cfr10I RCCGGY 1 cut(s) 387
Cfr13I GGNCC 5 cut(s) 84, 92, 520, 539, 657
Csp6I GTAC 1 cut(s) 80
CviAII CATG 2 cut(s) 221, 419
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 3 cut(s) 6, 306, 467
DpnI GATC 3 cut(s) 74, 357, 534
DpnII GATC 3 cut(s) 72, 355, 532
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 3 cut(s) 15, 130, 389
EarI CTCTTC 3 cut(s) 15, 130, 389
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 84, 657
Eco57I CTGAAG 2 cut(s) 98, 380
EcoO109I RGGNCCY 1 cut(s) 657
EcoRII CCWGG 2 cut(s) 601, 652
EcoRV GATATC 1 cut(s) 442
FaeI CATG 2 cut(s) 224, 422
FaqI GGGAC 2 cut(s) 670, 698
FatI CATG 2 cut(s) 220, 418
FokI GGATG 2 cut(s) 275, 332
FspBI CTAG 1 cut(s) 587
GsaI CCCAGC 1 cut(s) 98
HaeIII GGCC 4 cut(s) 93, 455, 521, 540
HapII CCGG 1 cut(s) 388
Hin1II CATG 2 cut(s) 224, 422
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 3 cut(s) 247, 269, 583
HpaII CCGG 1 cut(s) 388
Hpy188I TCNGA 4 cut(s) 134, 360, 582, 702
Hpy188III TCNNGA 3 cut(s) 317, 437, 677
Hpy99I CGWCG 2 cut(s) 341, 350
HpyAV CCTTC 2 cut(s) 114, 312
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 4 cut(s) 205, 220, 617, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 518
HpyF3I CTNAG 3 cut(s) 6, 306, 467
HpySE526I ACGT 1 cut(s) 673
Hsp92II CATG 2 cut(s) 224, 422
KflI GGGWCCC 1 cut(s) 657
Kzo9I GATC 3 cut(s) 72, 355, 532
LmnI GCTCC 2 cut(s) 103, 123
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 1 cut(s) 174
MalI GATC 3 cut(s) 74, 357, 534
MboI GATC 3 cut(s) 72, 355, 532
MboII GAAGA 9 cut(s) 32, 104, 117, 305, 362, 406, 487, 569, 647
MflI RGATCY 1 cut(s) 532
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 2 cut(s) 152, 486
MluNI TGGCCA 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 6 cut(s) 10, 133, 186, 325, 334, 390
Mox20I TGGCCA 1 cut(s) 455
MscI TGGCCA 1 cut(s) 455
MseI TTAA 1 cut(s) 663
Msp20I TGGCCA 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 2 cut(s) 603, 654
MvaI CCWGG 2 cut(s) 603, 654
MwoI GCNNNNNNNGC 2 cut(s) 202, 518
NdeII GATC 3 cut(s) 72, 355, 532
NlaIII CATG 2 cut(s) 224, 422
NlaIV GGNNCC 3 cut(s) 513, 658, 659
PcsI WCGNNNNNNNCGW 3 cut(s) 336, 342, 345
PfeI GAWTC 3 cut(s) 247, 269, 583
PpuMI RGGWCCY 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 601, 652
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 2 cut(s) 601, 652
PspN4I GGNNCC 3 cut(s) 513, 658, 659
PspPI GGNCC 5 cut(s) 84, 92, 520, 539, 657
PspPPI RGGWCCY 1 cut(s) 657
PsrI GAACNNNNNNTAC 2 cut(s) 396, 428
PsuI RGATCY 1 cut(s) 532
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 663
Sau3AI GATC 3 cut(s) 72, 355, 532
Sau96I GGNCC 5 cut(s) 84, 92, 520, 539, 657
ScrFI CCNGG 2 cut(s) 603, 654
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 2 cut(s) 84, 657
SmlI CTYRAG 2 cut(s) 145, 257
SmoI CTYRAG 2 cut(s) 145, 257
Sse9I AATT 2 cut(s) 152, 486
SsiI CCGC 1 cut(s) 595
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 2 cut(s) 601, 652
TaiI ACGT 1 cut(s) 676
TaqI TCGA 1 cut(s) 623
TasI AATT 2 cut(s) 152, 486
TfiI GAWTC 3 cut(s) 247, 269, 583
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 2 cut(s) 565, 619
TspDTI ATGAA 1 cut(s) 648
TspGWI ACGGA 2 cut(s) 97, 706
TspRI CASTG 2 cut(s) 565, 619
VpaK11BI GGWCC 2 cut(s) 84, 657
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.