MD06G1031100.v1.1

Uncharacterized conserved domain (SAYSvFN)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010199
Physical Location & Seq
Forward (+)
3746394 .. 3751955
5562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1031100.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGGCGAGATTACTGAGGAGGAAGAGCTGATACCCATTTCGAATAACAGCAGCATCGTCAACGGCGACGAGGAGTTTGAGAAGAAGAAGAAGAAGAAGGTGGAGATCGTTGTGAGAACCATTGGCCCAGCTCCCCCTTCTCGTCTCCAAGTCCCCTCATCCATCACAGTGCATGATTTGAGAAAATTGATAGCTGAGAATGGCCACTTACCAATTGAGAGGCTGAGGCTTATTTTGCGGGGCAATGTATTGCATGATAGCAAAAGTGCACAAGATGTGTACCTACAACTCAGTGACGGTGATTCCCTAATTGTTGCTGTCAAACCAAAGCCACCAGTTAAACATCTTAGGGATGGATTTGATGATGACGATGATGATGAAGATCTGTTTCAGCTTCCACTGTCATCGAGCCCATGGAAGAGAGCATTGTACAGTTTTCTACATGATAAGATGAAGCTCCCTGATATCCTTTTGATGGCAATTTTCTCTCTCAGTCTGAAGATGTGGGTAATTATCATTATGTGGTTTATTTTCGCACCTGTTGCCCACAGATGGGACATTGGCCCTTTATATATACTTGCCACAGGTTTTGTTGTCATCCTCGTGAATCTTGGAAAGCGGCAACCTGGCGATGTCAGTGCGTATTCTATTTTCAATGAAGATTTTAGAGAGCTTCCGGGGACTCTTAATGCCGAACGTCTTGATGGTGACATACGAACAGGTCAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAYSvFN ubiquitin

Protein Analysis

244

Amino Acids

27.47

Weight (kDa)

5.42

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 49 - 109 3.4e-07 Ubiquitin family
SAYSvFN PF10260 172 - 238 7.1e-25 Uncharacterized conserved domain (SAYSvFN)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35360 AT2G35360 AT2G35360
fragaria_vesca FvH4_5g21620 FvH4_5g21620 FvH4_5g21620
malus_domestica MD06G1031100.v1.1
prunus_persica Prupe.5G041200_v2.0.a1 Prupe.5G041200_v2.0.a1
pyrus_communis pycom06g02590
rosa_chinensis RchiOBHm_Chr7g0209081
rosa_laevigata RLG00000003169
rosa_multiflora Rmu_co8263433.1_g000001
rosa_roxburghii Rroxscaffold_3G00249770
rosa_rugosa Rorug07G0108800 Rorug07G0108900
rosa_samantha Rh7AG243300 Rh7BG238300 Rh7CG258500 Rh7DG250300
rosa_wichuraiana Rw7G020620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 238, 619
AcoI YGGCCR 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 2 cut(s) 281, 431
AfiI CCNNNNNNNGG 3 cut(s) 475, 552, 553
AgsI TTSAA 1 cut(s) 655
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 6 cut(s) 28, 131, 194, 394, 457, 673
AluI AGCT 6 cut(s) 28, 131, 194, 394, 457, 673
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 1 cut(s) 149
Alw44I GTGCAC 1 cut(s) 267
AoxI GGCC 3 cut(s) 124, 202, 562
ApaLI GTGCAC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 51
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspS9I GGNCC 2 cut(s) 125, 563
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 2 cut(s) 311, 719
AsuII TTCGAA 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 204
BanII GRGCYC 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BauI CACGAG 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 271
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 63
BccI CCATC 5 cut(s) 170, 347, 469, 546, 698
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 1 cut(s) 627
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 1 cut(s) 149
BglII AGATCT 1 cut(s) 382
BisI GCNGC 2 cut(s) 52, 620
BlsI GCNGC 2 cut(s) 53, 621
Bme1390I CCNGG 2 cut(s) 627, 678
BmgT120I GGNCC 2 cut(s) 125, 563
BmrFI CCNGG 2 cut(s) 627, 678
BmsI GCATC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 224
Bpu14I TTCGAA 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 413, 677
Bsc4I CCNNNNNNNGG 3 cut(s) 475, 552, 553
Bse1I ACTGG 1 cut(s) 335
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 381
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 2 cut(s) 413, 677
BseGI GGATG 3 cut(s) 158, 358, 597
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 3 cut(s) 475, 552, 553
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 5 cut(s) 6, 186, 215, 304, 505
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 2 cut(s) 32, 86
BseSI GKGCMC 1 cut(s) 271
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 127
BshFI GGCC 3 cut(s) 126, 204, 564
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 3 cut(s) 137, 569, 694
BslI CCNNNNNNNGG 3 cut(s) 475, 552, 553
BsmAI GTCTC 1 cut(s) 149
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 3 cut(s) 137, 569, 694
BsnI GGCC 3 cut(s) 126, 204, 564
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 271, 413
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 2 cut(s) 105, 382
Bsp19I CCATGG 1 cut(s) 413
BspACI CCGC 2 cut(s) 238, 619
BspANI GGCC 3 cut(s) 126, 204, 564
BspCNI CTCAG 5 cut(s) 7, 187, 216, 303, 504
BspQI GCTCTTC 1 cut(s) 18
BspT104I TTCGAA 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 250
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 413, 677
BssMI GATC 2 cut(s) 105, 382
BssSI CACGAG 1 cut(s) 602
BssT1I CCWWGG 1 cut(s) 413
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 4 cut(s) 169, 299, 402, 434
Bst6I CTCTTC 2 cut(s) 18, 413
BstAPI GCANNNNNTGC 1 cut(s) 542
BstAUI TGTACA 1 cut(s) 429
BstBI TTCGAA 1 cut(s) 41
BstDEI CTNAG 6 cut(s) 15, 195, 224, 290, 347, 491
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 3 cut(s) 158, 358, 597
BstKTI GATC 2 cut(s) 108, 385
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 2 cut(s) 105, 382
BstMWI GCNNNNNNNGC 2 cut(s) 235, 542
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 625, 676
BstSLI GKGCMC 1 cut(s) 271
BstV1I GCAGC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 3 cut(s) 126, 204, 564
BtgI CCRYGG 1 cut(s) 413
BtgZI GCGATG 1 cut(s) 645
BtsCI GGATG 3 cut(s) 158, 358, 597
BtsIMutI CAGTG 4 cut(s) 174, 298, 398, 643
Cfr13I GGNCC 2 cut(s) 125, 563
Csp6I GTAC 2 cut(s) 280, 430
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 4 cut(s) 173, 254, 414, 443
CviQI GTAC 2 cut(s) 280, 430
DdeI CTNAG 6 cut(s) 15, 195, 224, 290, 347, 491
DpnI GATC 2 cut(s) 107, 384
DpnII GATC 2 cut(s) 105, 382
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 18, 413
EarI CTCTTC 2 cut(s) 18, 413
Eco130I CCWWGG 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 625
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 413
EcoT38I GRGCYC 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 4 cut(s) 176, 257, 417, 446
FaiI YATR 9 cut(s) 174, 255, 415, 444, 521, 571, 573, 575, 713
FaqI GGGAC 3 cut(s) 137, 569, 694
FatI CATG 4 cut(s) 172, 253, 413, 442
FauI CCCGC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 52, 620
FokI GGATG 3 cut(s) 145, 365, 584
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 2 cut(s) 52, 620
GluI GCNGC 2 cut(s) 52, 620
GsaI CCCAGC 1 cut(s) 131
HaeIII GGCC 3 cut(s) 126, 204, 564
HapII CCGG 1 cut(s) 677
Hin1II CATG 4 cut(s) 176, 257, 417, 446
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 3 cut(s) 302, 607, 682
HpaII CCGG 1 cut(s) 677
HphI GGTGA 2 cut(s) 311, 719
Hpy166II GTNNAC 3 cut(s) 61, 269, 280
Hpy188I TCNGA 1 cut(s) 498
Hpy188III TCNNGA 2 cut(s) 604, 701
Hpy8I GTNNAC 3 cut(s) 61, 269, 280
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 91, 147
HpyCH4III ACNGT 4 cut(s) 169, 299, 402, 434
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 3 cut(s) 172, 253, 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 542
HpyF3I CTNAG 6 cut(s) 15, 195, 224, 290, 347, 491
HpySE526I ACGT 1 cut(s) 697
Hsp92II CATG 4 cut(s) 176, 257, 417, 446
Kzo9I GATC 2 cut(s) 105, 382
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 2 cut(s) 136, 462
LpnPI CCDG 9 cut(s) 141, 348, 474, 552, 570, 612, 639, 690, 705
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 1 cut(s) 63
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 2 cut(s) 293, 707
MalI GATC 2 cut(s) 107, 384
MboI GATC 2 cut(s) 105, 382
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 271, 413
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 5 cut(s) 185, 213, 309, 480, 510
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 1 cut(s) 676
MnlI CCTC 7 cut(s) 10, 13, 64, 166, 213, 219, 611
Mox20I TGGCCA 1 cut(s) 204
MscI TGGCCA 1 cut(s) 204
MseI TTAA 2 cut(s) 339, 687
MslI CAYNNNNRTG 2 cut(s) 167, 602
Msp20I TGGCCA 1 cut(s) 204
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 2 cut(s) 627, 678
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 2 cut(s) 235, 542
NciI CCSGG 1 cut(s) 678
NcoI CCATGG 1 cut(s) 413
NdeII GATC 2 cut(s) 105, 382
NlaIII CATG 4 cut(s) 176, 257, 417, 446
NmuCI GTSAC 2 cut(s) 293, 707
NspV TTCGAA 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 302, 607
PkrI GCNGC 2 cut(s) 53, 621
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
Psp6I CCWGG 1 cut(s) 625
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 625
PspPI GGNCC 2 cut(s) 125, 563
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 2 cut(s) 281, 431
RsaNI GTAC 2 cut(s) 280, 430
RseI CAYNNNNRTG 2 cut(s) 167, 602
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 339, 687
SatI GCNGC 2 cut(s) 52, 620
Sau3AI GATC 2 cut(s) 105, 382
Sau96I GGNCC 2 cut(s) 125, 563
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 2 cut(s) 627, 678
SduI GDGCHC 2 cut(s) 271, 413
SfaNI GCATC 1 cut(s) 63
SfuI TTCGAA 1 cut(s) 41
SmiMI CAYNNNNRTG 2 cut(s) 167, 602
Sse9I AATT 5 cut(s) 185, 213, 309, 480, 510
SsiI CCGC 2 cut(s) 238, 619
StyD4I CCNGG 2 cut(s) 625, 676
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 4 cut(s) 169, 299, 402, 434
TaiI ACGT 1 cut(s) 700
TaqI TCGA 2 cut(s) 41, 407
TasI AATT 5 cut(s) 185, 213, 309, 480, 510
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 622
TfiI GAWTC 2 cut(s) 302, 607
Tru1I TTAA 2 cut(s) 339, 687
Tru9I TTAA 2 cut(s) 339, 687
TscAI CASTG 4 cut(s) 174, 298, 405, 643
TseFI GTSAC 2 cut(s) 293, 707
TseI GCWGC 1 cut(s) 51
Tsp45I GTSAC 2 cut(s) 293, 707
TspDTI ATGAA 3 cut(s) 393, 467, 672
TspRI CASTG 4 cut(s) 174, 298, 405, 643
VneI GTGCAC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.