pycom06g02590

Uncharacterized conserved domain (SAYSvFN)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010199
Physical Location & Seq
Forward (+)
3119086 .. 3122755
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g02590.4

Sequence Viewer

Length: 735 bp
ATGGGCGAGATTACTGAGGAGGAAGAGCTGATACCCATTTCGAATAACAGCAGCATCGTCAATGGCGACGAGGAGTTTGAGAAGAAGAAGAAGAAGAAGGTGGAGATCGTTGTGAGAACCATCGGCCCAGCTCCCCCTTCTCGTCTCCAAGTCCCCTCATCCATCACAGTGCATGATTTGAGAAAATTGATAGCTGAGAATGGCCACTTACCAATTGAGAGGCTGAGGCTTATTTTGCGAGGCAATGTATTGCATGATAGCAAAAGTGCGCAAGATGAGTACCTACAACTCAGTGACGGTGATTCCCTAATTGTTGCTGTCAAACCAAAGCCGCCAGTTAAACATCTTAGGGATGGATTTGATGATGACGATGATGATGAAGATCTGAAGTTTCAGCTTCCGCTGTCATCAAGCCCGTGGAAGAGGGCATTGTACAGTTTTCTACATGATAAGATGAAGCTCCCTGATATCCTTTTGATGGCAATTTTCTCTCTCAGTCTGAAGATGTGGGTAATTATCATTATGTGGTTTATTTTCGCACCTGTTGCCCACAGATGGGACATTGGCCCTTTATATATACTTGCCACAGGTTTTGTTGTCATCCTTGTGAATCTTGGAAAGCGGCAACCTGGCGATGTCAGTGCGTATTCTATTTTCAATGAAGATTTTAGAGAGCTCCCCGGGACTCTTAATGCTGAACGTCTTGATGGGGACATACGAACAGGTCAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAYSvFN ubiquitin

Protein Analysis

245

Amino Acids

27.63

Weight (kDa)

5.43

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35360 AT2G35360 AT2G35360
fragaria_vesca FvH4_5g21620 FvH4_5g21620 FvH4_5g21620
malus_domestica MD06G1031100.v1.1
prunus_persica Prupe.5G041200_v2.0.a1 Prupe.5G041200_v2.0.a1
pyrus_communis pycom06g02590
rosa_chinensis RchiOBHm_Chr7g0209081
rosa_laevigata RLG00000003169
rosa_multiflora Rmu_co8263433.1_g000001
rosa_roxburghii Rroxscaffold_3G00249770
rosa_rugosa Rorug07G0108800 Rorug07G0108900
rosa_samantha Rh7AG243300 Rh7BG238300 Rh7CG258500 Rh7DG250300
rosa_wichuraiana Rw7G020620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 270
AciI CCGC 3 cut(s) 332, 401, 622
AcoI YGGCCR 1 cut(s) 202
AcuI CTGAAG 2 cut(s) 407, 521
AfaI GTAC 2 cut(s) 281, 434
AfiI CCNNNNNNNGG 3 cut(s) 478, 555, 556
AgsI TTSAA 1 cut(s) 658
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 6 cut(s) 28, 131, 194, 397, 460, 676
AluI AGCT 6 cut(s) 28, 131, 194, 397, 460, 676
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 3 cut(s) 124, 202, 565
ApeKI GCWGC 1 cut(s) 51
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 2 cut(s) 125, 566
AsuC2I CCSGG 2 cut(s) 681, 682
AsuHPI GGTGA 1 cut(s) 311
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 680
BalI TGGCCA 1 cut(s) 204
BanII GRGCYC 1 cut(s) 678
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
Bbv12I GWGCWC 1 cut(s) 678
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 63
BccI CCATC 6 cut(s) 128, 170, 347, 472, 549, 701
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BciT130I CCWGG 1 cut(s) 630
BcnI CCSGG 2 cut(s) 681, 682
BcoDI GTCTC 1 cut(s) 149
BglII AGATCT 1 cut(s) 382
BisI GCNGC 3 cut(s) 52, 332, 623
BlsI GCNGC 3 cut(s) 53, 333, 624
Bme1390I CCNGG 3 cut(s) 630, 681, 682
BmeT110I CYCGRG 1 cut(s) 680
BmgT120I GGNCC 2 cut(s) 125, 566
BmrFI CCNGG 3 cut(s) 630, 681, 682
BmsI GCATC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 224
Bpu14I TTCGAA 1 cut(s) 41
BpuMI CCSGG 2 cut(s) 681, 682
BsaBI GATNNNNATC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 416, 679, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 478, 555, 556
Bse1I ACTGG 1 cut(s) 335
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 381
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 3 cut(s) 416, 679, 680
BseGI GGATG 3 cut(s) 158, 358, 600
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 3 cut(s) 478, 555, 556
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 5 cut(s) 6, 186, 215, 304, 508
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 2 cut(s) 32, 86
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 127
BshFI GGCC 3 cut(s) 126, 204, 567
BsiHKAI GWGCWC 1 cut(s) 678
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 1 cut(s) 681
BslFI GGGAC 4 cut(s) 137, 572, 697, 725
BslI CCNNNNNNNGG 3 cut(s) 478, 555, 556
BsmAI GTCTC 1 cut(s) 149
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 4 cut(s) 137, 572, 697, 725
BsnI GGCC 3 cut(s) 126, 204, 567
BsoBI CYCGRG 1 cut(s) 680
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 678
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 2 cut(s) 105, 382
BspACI CCGC 3 cut(s) 332, 401, 622
BspANI GGCC 3 cut(s) 126, 204, 567
BspCNI CTCAG 5 cut(s) 7, 187, 216, 303, 507
BspQI GCTCTTC 1 cut(s) 18
BspT104I TTCGAA 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 250
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 416, 679, 680
BssMI GATC 2 cut(s) 105, 382
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 3 cut(s) 169, 299, 437
Bst6I CTCTTC 2 cut(s) 18, 416
BstAPI GCANNNNNTGC 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 41
BstDEI CTNAG 6 cut(s) 15, 195, 224, 290, 347, 494
BstDSI CCRYGG 1 cut(s) 416
BstF5I GGATG 3 cut(s) 158, 358, 600
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 2 cut(s) 108, 385
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 2 cut(s) 105, 382
BstMWI GCNNNNNNNGC 2 cut(s) 235, 545
BstNI CCWGG 1 cut(s) 630
BstSCI CCNGG 3 cut(s) 628, 679, 680
BstV1I GCAGC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 3 cut(s) 126, 204, 567
BtgI CCRYGG 1 cut(s) 416
BtgZI GCGATG 1 cut(s) 648
BtsCI GGATG 3 cut(s) 158, 358, 600
BtsIMutI CAGTG 3 cut(s) 174, 298, 646
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 125, 566
Cfr9I CCCGGG 1 cut(s) 680
Csp6I GTAC 2 cut(s) 280, 433
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 3 cut(s) 173, 254, 446
CviQI GTAC 2 cut(s) 280, 433
DdeI CTNAG 6 cut(s) 15, 195, 224, 290, 347, 494
DpnI GATC 2 cut(s) 107, 384
DpnII GATC 2 cut(s) 105, 382
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 18, 416
EarI CTCTTC 2 cut(s) 18, 416
Ecl136II GAGCTC 1 cut(s) 676
Eco24I GRGCYC 1 cut(s) 678
Eco32I GATATC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 676
Eco57I CTGAAG 2 cut(s) 407, 521
Eco88I CYCGRG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 628
EcoRV GATATC 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 678
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 3 cut(s) 176, 257, 449
FaiI YATR 8 cut(s) 174, 255, 447, 524, 574, 576, 578, 716
FaqI GGGAC 4 cut(s) 137, 572, 697, 725
FatI CATG 3 cut(s) 172, 253, 445
Fnu4HI GCNGC 3 cut(s) 52, 332, 623
FokI GGATG 3 cut(s) 145, 365, 587
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 3 cut(s) 52, 332, 623
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 52, 332, 623
GsaI CCCAGC 1 cut(s) 131
HaeIII GGCC 3 cut(s) 126, 204, 567
HapII CCGG 1 cut(s) 681
HhaI GCGC 1 cut(s) 271
Hin1II CATG 3 cut(s) 176, 257, 449
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 3 cut(s) 302, 610, 685
HpaII CCGG 1 cut(s) 681
HphI GGTGA 1 cut(s) 311
Hpy188I TCNGA 2 cut(s) 387, 501
Hpy188III TCNNGA 1 cut(s) 704
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 91, 147
HpyCH4III ACNGT 3 cut(s) 169, 299, 437
HpyCH4IV ACGT 1 cut(s) 700
HpyCH4V TGCA 2 cut(s) 172, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 545
HpyF3I CTNAG 6 cut(s) 15, 195, 224, 290, 347, 494
HpySE526I ACGT 1 cut(s) 700
Hsp92II CATG 3 cut(s) 176, 257, 449
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 2 cut(s) 105, 382
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 3 cut(s) 136, 465, 681
LpnPI CCDG 9 cut(s) 141, 348, 477, 555, 573, 615, 642, 694, 708
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 1 cut(s) 63
MaeII ACGT 1 cut(s) 700
MaeIII GTNAC 1 cut(s) 293
MalI GATC 2 cut(s) 107, 384
MboI GATC 2 cut(s) 105, 382
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 1 cut(s) 678
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 5 cut(s) 185, 213, 309, 483, 513
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 1 cut(s) 679
MnlI CCTC 8 cut(s) 10, 13, 64, 166, 213, 219, 233, 417
Mox20I TGGCCA 1 cut(s) 204
MscI TGGCCA 1 cut(s) 204
MseI TTAA 2 cut(s) 339, 690
MslI CAYNNNNRTG 2 cut(s) 167, 605
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 1 cut(s) 681
MspR9I CCNGG 3 cut(s) 630, 681, 682
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 630
MwoI GCNNNNNNNGC 2 cut(s) 235, 545
NciI CCSGG 2 cut(s) 681, 682
NdeII GATC 2 cut(s) 105, 382
NlaIII CATG 3 cut(s) 176, 257, 449
NmuCI GTSAC 1 cut(s) 293
NsbI TGCGCA 1 cut(s) 270
NspV TTCGAA 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 302, 610
PkrI GCNGC 3 cut(s) 53, 333, 624
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
Psp124BI GAGCTC 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 628
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 628
PspPI GGNCC 2 cut(s) 125, 566
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 2 cut(s) 281, 434
RsaNI GTAC 2 cut(s) 280, 433
RseI CAYNNNNRTG 2 cut(s) 167, 605
SacI GAGCTC 1 cut(s) 678
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 339, 690
SatI GCNGC 3 cut(s) 52, 332, 623
Sau3AI GATC 2 cut(s) 105, 382
Sau96I GGNCC 2 cut(s) 125, 566
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 3 cut(s) 630, 681, 682
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 1 cut(s) 63
SfuI TTCGAA 1 cut(s) 41
SmaI CCCGGG 1 cut(s) 682
SmiMI CAYNNNNRTG 2 cut(s) 167, 605
Sse9I AATT 5 cut(s) 185, 213, 309, 483, 513
SsiI CCGC 3 cut(s) 332, 401, 622
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 3 cut(s) 628, 679, 680
TaaI ACNGT 3 cut(s) 169, 299, 437
TaiI ACGT 1 cut(s) 703
TaqI TCGA 1 cut(s) 41
TasI AATT 5 cut(s) 185, 213, 309, 483, 513
TatI WGTACW 1 cut(s) 432
TauI GCSGC 2 cut(s) 334, 625
TfiI GAWTC 2 cut(s) 302, 610
Tru1I TTAA 2 cut(s) 339, 690
Tru9I TTAA 2 cut(s) 339, 690
TscAI CASTG 3 cut(s) 174, 298, 646
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 51
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 3 cut(s) 393, 470, 675
TspMI CCCGGG 1 cut(s) 680
TspRI CASTG 3 cut(s) 174, 298, 646
XmaI CCCGGG 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.