AT2G35450

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015263
Physical Location & Seq
Reverse (-)
14902444 .. 14905388
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35450.1

Sequence Viewer

Length: 1041 bp
ATGGCTGTGACTCTGAGATTAACGAATTCTTTAGCATTTTCCAGTCATCCTCTAACGTCGTCCGTACTGTTCCGAGGAAGCAAATCGATCGGTACTATTACTAAGATAGCATCAACAATCAGGACGGTAAAAATGGCGGCTTTTGCTGGGTCTGACTCAGAAACAACGTCGTCTACCTCGAGAGTTATCGATTCTCATCTCCACATTTGGGCTTCTCCTCAAGAGGCCGAGACATATCCTTATTTTCCTGGCCAAGAACCTACTTTGACTGGTGATGTCAATTTCTTGCTTAAGAACATGGAAGAGGCAAGAGTAGATGGAGCTCTCATTGTGCAACCCATAAATCACAAGTTTGACCATTCTTTAGTAACAAGTGTTCTGAAGAGATACCCATCAAAGTTTGTCGGTTGTTGCCTTGCTAATCCTGCAGAAGATGGCAGTGGAATTACGCACCTTGAGAATCTTGTTTTAGAGAGTAATTATCGTGCTGTTCGGTTTAATCCCTACTTGTGGCCATCGGGTCAGAAGATGACAAATGCTGTTGGTAAAGCCTTGTTCTCTAAAGCAGGGGAGCTTTGTGTGCCTGTTGGCTTCATGTGCATGAAGGGTCTAGATCTTCACATTGCAGAAATTGAGGAACTTTGCACAGAATTTCCAAAGACAACTGTTTTACTAGATCACGCTGGATTCTGCAAAGTACCAGAAAGTGGTGAGGCAAAGCTTGCTTATTCTCAACTCATGAAGCTGTCTAGATTTCCACAGGTATATGTGAAATTCAGTGCTCTATTCAGGATCTCAAGAACCGGCTTCCCGTATCAGGACCTATCGCCTCTCCTATCTCAACTTGTGTCCCATTTTGGGGCAAATCGTGTCATGTGGGGCAGTGACTTCCCATTTGTTGTTCTTGAATGTGGATATAAAGAAGCTAAAGAAGCTGTGACTATCATTGCAAAAGAAGCATCCTTGTCAAGTTCTGAGATGGACTGGATTCTGGGAAAGACTCTGATGCAGCTCTTCCCTGGACAATGGGTTCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

346

Amino Acids

38.16

Weight (kDa)

7.1

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 63 - 334 1.5e-28 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 173
AciI CCGC 1 cut(s) 137
AclWI GGATC 1 cut(s) 800
AcoI YGGCCR 2 cut(s) 250, 512
AcsI RAATTY 3 cut(s) 25, 650, 773
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 3 cut(s) 66, 94, 699
AfiI CCNNNNNNNGG 6 cut(s) 208, 510, 707, 817, 858, 859
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 1 cut(s) 908
AjnI CCWGG 2 cut(s) 247, 1018
AloI GAACNNNNNNTCC 1 cut(s) 1014
AluBI AGCT 7 cut(s) 323, 574, 721, 745, 926, 935, 1012
AluI AGCT 7 cut(s) 323, 574, 721, 745, 926, 935, 1012
Alw21I GWGCWC 2 cut(s) 325, 784
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 1 cut(s) 800
Ama87I CYCGRG 1 cut(s) 178
AoxI GGCC 3 cut(s) 225, 250, 512
ApeKI GCWGC 1 cut(s) 1009
ApoI RAATTY 3 cut(s) 25, 650, 773
ArsI GACNNNNNNTTYG 2 cut(s) 878, 910
AspS9I GGNCC 1 cut(s) 820
AsuHPI GGTGA 2 cut(s) 284, 722
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 820
BalI TGGCCA 2 cut(s) 252, 514
BanII GRGCYC 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 325, 784
BbvI GCAGC 1 cut(s) 1021
BccI CCATC 5 cut(s) 311, 400, 428, 523, 973
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 2 cut(s) 249, 1020
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 3 cut(s) 611, 674, 750
BfmI CTRYAG 1 cut(s) 426
BfrI CTTAAG 1 cut(s) 290
BglII AGATCT 1 cut(s) 613
BisI GCNGC 2 cut(s) 138, 1010
BlsI GCNGC 2 cut(s) 139, 1011
Bme1390I CCNGG 2 cut(s) 249, 1020
Bme18I GGWCC 1 cut(s) 820
BmeT110I CYCGRG 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 820
BmrFI CCNGG 2 cut(s) 249, 1020
BmsI GCATC 3 cut(s) 119, 968, 996
BpuEI CTTGAG 3 cut(s) 204, 476, 781
Bsa29I ATCGAT 2 cut(s) 86, 189
BsaBI GATNNNNATC 2 cut(s) 195, 391
BsaJI CCNNGG 2 cut(s) 73, 1018
Bsc4I CCNNNNNNNGG 6 cut(s) 208, 510, 707, 817, 858, 859
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 3 cut(s) 42, 274, 989
Bse3DI GCAATG 2 cut(s) 621, 945
Bse8I GATNNNNATC 2 cut(s) 195, 391
BseBI CCWGG 2 cut(s) 249, 1020
BseCI ATCGAT 2 cut(s) 86, 189
BseDI CCNNGG 2 cut(s) 73, 1018
BseGI GGATG 2 cut(s) 46, 959
BseJI GATNNNNATC 2 cut(s) 195, 391
BseLI CCNNNNNNNGG 6 cut(s) 208, 510, 707, 817, 858, 859
BseMI GCAATG 2 cut(s) 621, 945
BseMII CTCAG 3 cut(s) 5, 171, 966
BseNI ACTGG 3 cut(s) 42, 274, 989
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 1 cut(s) 1021
BseYI CCCAGC 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 227, 252, 514
BshVI ATCGAT 2 cut(s) 86, 189
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 2 cut(s) 325, 784
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 1 cut(s) 804
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 6 cut(s) 208, 510, 707, 817, 858, 859
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 3 cut(s) 227, 252, 514
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 325, 784
Bsp143I GATC 4 cut(s) 87, 613, 676, 792
BspACI CCGC 1 cut(s) 137
BspANI GGCC 3 cut(s) 227, 252, 514
BspCNI CTCAG 3 cut(s) 6, 170, 967
BspDI ATCGAT 2 cut(s) 86, 189
BspHI TCATGA 1 cut(s) 738
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 1 cut(s) 800
BspQI GCTCTTC 1 cut(s) 1019
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 621, 945
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 3 cut(s) 42, 274, 989
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 2 cut(s) 73, 1018
BssMI GATC 4 cut(s) 87, 613, 676, 792
Bst2UI CCWGG 2 cut(s) 249, 1020
Bst4CI ACNGT 3 cut(s) 69, 127, 667
Bst6I CTCTTC 3 cut(s) 297, 377, 1019
BstAFI CTTAAG 1 cut(s) 290
BstAPI GCANNNNNTGC 1 cut(s) 722
BstC8I GCNNGC 1 cut(s) 723
BstDEI CTNAG 4 cut(s) 14, 102, 157, 975
BstF5I GGATG 2 cut(s) 46, 959
BstKTI GATC 4 cut(s) 90, 616, 679, 795
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 4 cut(s) 87, 613, 676, 792
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 7 cut(s) 143, 425, 580, 597, 722, 932, 956
BstNI CCWGG 2 cut(s) 249, 1020
BstSCI CCNGG 2 cut(s) 247, 1018
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 1021
BstX2I RGATCY 2 cut(s) 613, 792
BstYI RGATCY 2 cut(s) 613, 792
Bsu15I ATCGAT 2 cut(s) 86, 189
BsuRI GGCC 3 cut(s) 227, 252, 514
BsuTUI ATCGAT 2 cut(s) 86, 189
BtsCI GGATG 2 cut(s) 46, 959
BtsI GCAGTG 2 cut(s) 445, 889
BtsIMutI CAGTG 3 cut(s) 445, 784, 889
Cac8I GCNNGC 1 cut(s) 723
CciI TCATGA 1 cut(s) 738
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 1 cut(s) 820
ClaI ATCGAT 2 cut(s) 86, 189
Csp6I GTAC 3 cut(s) 65, 93, 698
CviAII CATG 5 cut(s) 298, 595, 601, 739, 874
CviQI GTAC 3 cut(s) 65, 93, 698
DdeI CTNAG 4 cut(s) 14, 102, 157, 975
DpnI GATC 4 cut(s) 89, 615, 678, 794
DpnII GATC 4 cut(s) 87, 613, 676, 792
EaeI YGGCCR 2 cut(s) 250, 512
Eam1104I CTCTTC 3 cut(s) 297, 377, 1019
EarI CTCTTC 3 cut(s) 297, 377, 1019
Ecl136II GAGCTC 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 325
Eco47I GGWCC 1 cut(s) 820
Eco53kI GAGCTC 1 cut(s) 323
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 820
EcoRI GAATTC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 247, 1018
EcoT38I GRGCYC 1 cut(s) 325
FaeI CATG 5 cut(s) 301, 598, 604, 742, 877
FaqI GGGAC 1 cut(s) 835
FatI CATG 5 cut(s) 297, 594, 600, 738, 873
FblI GTMKAC 1 cut(s) 173
Fnu4HI GCNGC 2 cut(s) 138, 1010
FokI GGATG 2 cut(s) 33, 946
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 138, 1010
FspBI CTAG 3 cut(s) 611, 674, 750
GluI GCNGC 2 cut(s) 138, 1010
GsaI CCCAGC 1 cut(s) 150
HaeIII GGCC 3 cut(s) 227, 252, 514
HapII CCGG 1 cut(s) 804
Hin1II CATG 5 cut(s) 301, 598, 604, 742, 877
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 7 cut(s) 10, 155, 191, 460, 687, 988, 1000
HpaII CCGG 1 cut(s) 804
HphI GGTGA 2 cut(s) 284, 722
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 8 cut(s) 15, 74, 154, 160, 381, 525, 976, 1005
Hpy8I GTNNAC 1 cut(s) 174
Hpy99I CGWCG 2 cut(s) 61, 172
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 3 cut(s) 69, 127, 667
HpyCH4IV ACGT 2 cut(s) 56, 167
HpyCH4V TGCA 8 cut(s) 334, 428, 600, 626, 645, 693, 950, 1009
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 425, 580, 597, 722, 932, 956
HpyF3I CTNAG 4 cut(s) 14, 102, 157, 975
HpySE526I ACGT 2 cut(s) 56, 167
Hsp92II CATG 5 cut(s) 301, 598, 604, 742, 877
Kzo9I GATC 4 cut(s) 87, 613, 676, 792
LguI GCTCTTC 1 cut(s) 1019
LmnI GCTCC 2 cut(s) 320, 571
Lsp1109I GCAGC 1 cut(s) 1021
LweI GCATC 3 cut(s) 119, 968, 996
MaeI CTAG 3 cut(s) 611, 674, 750
MaeII ACGT 2 cut(s) 56, 167
MaeIII GTNAC 4 cut(s) 7, 367, 884, 937
MalI GATC 4 cut(s) 89, 615, 678, 794
MboI GATC 4 cut(s) 87, 613, 676, 792
MboII GAAGA 7 cut(s) 314, 394, 443, 538, 608, 1006, 1025
MflI RGATCY 2 cut(s) 613, 792
MhlI GDGCHC 2 cut(s) 325, 784
MlsI TGGCCA 2 cut(s) 252, 514
MluCI AATT 7 cut(s) 25, 280, 444, 478, 630, 650, 773
MluNI TGGCCA 2 cut(s) 252, 514
MlyI GAGTC 3 cut(s) 4, 149, 994
MnlI CCTC 9 cut(s) 60, 68, 187, 217, 228, 298, 628, 706, 840
Mox20I TGGCCA 2 cut(s) 252, 514
MscI TGGCCA 2 cut(s) 252, 514
MseI TTAA 3 cut(s) 20, 291, 498
MslI CAYNNNNRTG 1 cut(s) 599
Msp20I TGGCCA 2 cut(s) 252, 514
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 804
MspR9I CCNGG 2 cut(s) 249, 1020
MvaI CCWGG 2 cut(s) 249, 1020
MwoI GCNNNNNNNGC 7 cut(s) 143, 425, 580, 597, 722, 932, 956
NdeII GATC 4 cut(s) 87, 613, 676, 792
NlaIII CATG 5 cut(s) 301, 598, 604, 742, 877
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 3 cut(s) 7, 884, 937
PaeR7I CTCGAG 1 cut(s) 178
PagI TCATGA 1 cut(s) 738
PciSI GCTCTTC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PfeI GAWTC 4 cut(s) 191, 460, 687, 988
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 2 cut(s) 139, 1011
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 3 cut(s) 4, 149, 994
PpsI GAGTC 3 cut(s) 4, 149, 994
PpuMI RGGWCCY 1 cut(s) 820
Psp124BI GAGCTC 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 820
Psp6I CCWGG 2 cut(s) 247, 1018
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 2 cut(s) 247, 1018
PspPI GGNCC 1 cut(s) 820
PspPPI RGGWCCY 1 cut(s) 820
PsrI GAACNNNNNNTAC 2 cut(s) 360, 392
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 2 cut(s) 613, 792
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 3 cut(s) 66, 94, 699
RsaNI GTAC 3 cut(s) 65, 93, 698
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 1019
SaqAI TTAA 3 cut(s) 20, 291, 498
SatI GCNGC 2 cut(s) 138, 1010
Sau3AI GATC 4 cut(s) 87, 613, 676, 792
Sau96I GGNCC 1 cut(s) 820
SchI GAGTC 3 cut(s) 4, 149, 994
ScrFI CCNGG 2 cut(s) 249, 1020
SduI GDGCHC 2 cut(s) 325, 784
SfaNI GCATC 3 cut(s) 119, 968, 996
SfcI CTRYAG 1 cut(s) 426
Sfr274I CTCGAG 1 cut(s) 178
SinI GGWCC 1 cut(s) 820
SlaI CTCGAG 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 5 cut(s) 178, 219, 290, 455, 796
SmoI CTYRAG 5 cut(s) 178, 219, 290, 455, 796
Sse9I AATT 7 cut(s) 25, 280, 444, 478, 630, 650, 773
SsiI CCGC 1 cut(s) 137
SspMI CTAG 3 cut(s) 611, 674, 750
SstI GAGCTC 1 cut(s) 325
StyD4I CCNGG 2 cut(s) 247, 1018
TaaI ACNGT 3 cut(s) 69, 127, 667
TaiI ACGT 2 cut(s) 59, 170
TaqI TCGA 3 cut(s) 86, 179, 189
TasI AATT 7 cut(s) 25, 280, 444, 478, 630, 650, 773
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 4 cut(s) 191, 460, 687, 988
Tru1I TTAA 3 cut(s) 20, 291, 498
Tru9I TTAA 3 cut(s) 20, 291, 498
TscAI CASTG 3 cut(s) 445, 784, 889
TseFI GTSAC 3 cut(s) 7, 884, 937
TseI GCWGC 1 cut(s) 1009
Tsp45I GTSAC 3 cut(s) 7, 884, 937
TspDTI ATGAA 3 cut(s) 583, 617, 755
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 3 cut(s) 445, 784, 889
Van91I CCANNNNNTGG 1 cut(s) 707
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 820
XapI RAATTY 3 cut(s) 25, 650, 773
XbaI TCTAGA 2 cut(s) 610, 749
XhoI CTCGAG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 173
XspI CTAG 3 cut(s) 611, 674, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.