Rw4G025400

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015263
Physical Location & Seq
Reverse (-)
51474670 .. 51477558
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025400.1

Sequence Viewer

Length: 987 bp
ATGGCAATGGCAGTGAGGCTAACACCTGTTCTGGCTCAGTTTAAACACTTTGCCTTCAGTTCAAGAGAAAACCAGTTCAGGGTCTCTGCTGCAGCAATGGCTACAGGTGGACCACAGAGCAGAACTAAAGTCATAGATTCACATCTCCATGTGTGGGCCTCACCTCAAGAGGCTGCTGATAAATATCCTTACTTTCCTGGGCAAGAACCCACTTTGCCTGGAGATGTCGATTTATTGCTTAAGTGTATGGAAGAAGCAGGTGTTGACGGTGCACTTATTGTGCAGCCTATTAATCACAAATTCGATCACTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGCGAGTGGGGTTAAGCAGCTTGAACATCTTATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCTTCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCCAAGGCAGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTTTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAAAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAATGCCATTTCCATATGAAGATGTATCTCACACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTCACCGGGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATAAAGGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTATCATCTTCAGAGTTGGAGTGGATCATGGGTAGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

328

Amino Acids

36.39

Weight (kDa)

6.56

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 45 - 322 2.1e-30 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AarI CACCTGC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 248
AclWI GGATC 1 cut(s) 944
AcsI RAATTY 2 cut(s) 299, 347
AcuI CTGAAG 3 cut(s) 40, 399, 906
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 2 cut(s) 79, 154
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 5 cut(s) 63, 331, 407, 448, 609
AjnI CCWGG 2 cut(s) 196, 217
AluBI AGCT 3 cut(s) 377, 403, 694
AluI AGCT 3 cut(s) 377, 403, 694
Alw21I GWGCWC 3 cut(s) 274, 733, 814
Alw26I GTCTC 1 cut(s) 88
Alw44I GTGCAC 2 cut(s) 270, 729
AlwI GGATC 1 cut(s) 944
AoxI GGCC 3 cut(s) 156, 461, 556
ApaLI GTGCAC 2 cut(s) 270, 729
ApeKI GCWGC 5 cut(s) 89, 92, 173, 283, 400
ApoI RAATTY 2 cut(s) 299, 347
AseI ATTAAT 1 cut(s) 291
AspS9I GGNCC 3 cut(s) 110, 156, 556
AsuC2I CCSGG 1 cut(s) 818
AsuHPI GGTGA 3 cut(s) 153, 328, 806
AvaII GGWCC 1 cut(s) 110
BaeGI GKGCMC 2 cut(s) 274, 733
Bbv12I GWGCWC 3 cut(s) 274, 733, 814
BbvI GCAGC 5 cut(s) 76, 104, 160, 295, 412
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 869
BciT130I CCWGG 2 cut(s) 198, 219
BciVI GTATCC 1 cut(s) 348
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 1 cut(s) 88
BfmI CTRYAG 2 cut(s) 90, 102
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 1 cut(s) 248
BfuI GTATCC 1 cut(s) 348
BisI GCNGC 5 cut(s) 90, 93, 174, 284, 401
BlsI GCNGC 5 cut(s) 91, 94, 175, 285, 402
Bme1390I CCNGG 3 cut(s) 198, 219, 818
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 110, 156, 556
BmrFI CCNGG 3 cut(s) 198, 219, 818
BmsI GCATC 1 cut(s) 373
BplI GAGNNNNNCTC 2 cut(s) 662, 694
BpmI CTGGAG 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 818
BsaBI GATNNNNATC 3 cut(s) 141, 183, 429
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 197, 510, 559
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 154
Bse1I ACTGG 3 cut(s) 73, 533, 900
Bse3DI GCAATG 3 cut(s) 12, 102, 507
Bse8I GATNNNNATC 3 cut(s) 141, 183, 429
BseBI CCWGG 2 cut(s) 198, 219
BseDI CCNNGG 3 cut(s) 197, 510, 559
BseJI GATNNNNATC 3 cut(s) 141, 183, 429
BseLI CCNNNNNNNGG 2 cut(s) 79, 154
BseMI GCAATG 3 cut(s) 12, 102, 507
BseMII CTCAG 2 cut(s) 50, 846
BseNI ACTGG 3 cut(s) 73, 533, 900
BseSI GKGCMC 2 cut(s) 274, 733
BseXI GCAGC 5 cut(s) 76, 104, 160, 295, 412
BsgI GTGCAG 2 cut(s) 302, 666
BshFI GGCC 3 cut(s) 158, 463, 558
BsiHKAI GWGCWC 3 cut(s) 274, 733, 814
BsiSI CCGG 1 cut(s) 817
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 2 cut(s) 79, 154
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 772
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 3 cut(s) 158, 463, 558
Bso31I GGTCTC 1 cut(s) 88
Bsp1286I GDGCHC 3 cut(s) 274, 733, 814
Bsp143I GATC 3 cut(s) 304, 625, 936
BspANI GGCC 3 cut(s) 158, 463, 558
BspCNI CTCAG 2 cut(s) 49, 847
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 1 cut(s) 944
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 88
BsrDI GCAATG 3 cut(s) 12, 102, 507
BsrI ACTGG 3 cut(s) 73, 533, 900
BssECI CCNNGG 3 cut(s) 197, 510, 559
BssMI GATC 3 cut(s) 304, 625, 936
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 198, 219
Bst4CI ACNGT 2 cut(s) 269, 786
Bst6I CTCTTC 1 cut(s) 968
BstAFI CTTAAG 1 cut(s) 239
BstDEI CTNAG 2 cut(s) 36, 855
BstKTI GATC 3 cut(s) 307, 628, 939
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 3 cut(s) 304, 625, 936
BstMWI GCNNNNNNNGC 5 cut(s) 98, 374, 383, 680, 881
BstNI CCWGG 2 cut(s) 198, 219
BstSCI CCNGG 3 cut(s) 196, 217, 816
BstSFI CTRYAG 2 cut(s) 90, 102
BstSLI GKGCMC 2 cut(s) 274, 733
BstV1I GCAGC 5 cut(s) 76, 104, 160, 295, 412
BstXI CCANNNNNNTGG 1 cut(s) 459
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 3 cut(s) 158, 463, 558
BtsI GCAGTG 2 cut(s) 18, 838
BtsIMutI CAGTG 4 cut(s) 18, 782, 838, 980
BveI ACCTGC 1 cut(s) 248
Cfr13I GGNCC 3 cut(s) 110, 156, 556
Csp6I GTAC 1 cut(s) 530
CviAII CATG 3 cut(s) 149, 823, 940
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 2 cut(s) 36, 855
DpnI GATC 3 cut(s) 306, 627, 938
DpnII GATC 3 cut(s) 304, 625, 936
DraI TTTAAA 3 cut(s) 43, 420, 690
Eam1104I CTCTTC 1 cut(s) 968
EarI CTCTTC 1 cut(s) 968
Eco130I CCWWGG 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 3 cut(s) 40, 399, 906
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 2 cut(s) 196, 217
EcoT14I CCWWGG 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 3 cut(s) 152, 826, 943
FaqI GGGAC 1 cut(s) 772
FatI CATG 3 cut(s) 148, 822, 939
FauNDI CATATG 1 cut(s) 763
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 5 cut(s) 90, 93, 174, 284, 401
Fsp4HI GCNGC 5 cut(s) 90, 93, 174, 284, 401
GluI GCNGC 5 cut(s) 90, 93, 174, 284, 401
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 3 cut(s) 158, 463, 558
HapII CCGG 1 cut(s) 817
Hin1II CATG 3 cut(s) 152, 826, 943
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 137, 970
HpaII CCGG 1 cut(s) 817
HphI GGTGA 3 cut(s) 153, 328, 806
Hpy166II GTNNAC 4 cut(s) 110, 265, 272, 731
Hpy188I TCNGA 2 cut(s) 577, 925
Hpy188III TCNNGA 5 cut(s) 63, 167, 699, 747, 967
Hpy8I GTNNAC 4 cut(s) 110, 265, 272, 731
HpyAV CCTTC 4 cut(s) 64, 474, 547, 615
HpyCH4III ACNGT 2 cut(s) 269, 786
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 374, 383, 680, 881
HpyF3I CTNAG 2 cut(s) 36, 855
Hsp92II CATG 3 cut(s) 152, 826, 943
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 304, 625, 936
LmnI GCTCC 1 cut(s) 872
Lsp1109I GCAGC 5 cut(s) 76, 104, 160, 295, 412
LweI GCATC 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 316
MalI GATC 3 cut(s) 306, 627, 938
MboI GATC 3 cut(s) 304, 625, 936
MboII GAAGA 6 cut(s) 263, 343, 392, 779, 912, 955
MhlI GDGCHC 3 cut(s) 274, 733, 814
MluCI AATT 3 cut(s) 299, 347, 579
MmeI TCCRAC 2 cut(s) 472, 909
MnlI CCTC 9 cut(s) 9, 163, 169, 174, 569, 577, 655, 664, 921
MseI TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
MslI CAYNNNNRTG 2 cut(s) 147, 457
MspA1I CMGCKG 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 1 cut(s) 817
MspR9I CCNGG 3 cut(s) 198, 219, 818
MssI GTTTAAAC 1 cut(s) 43
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 2 cut(s) 198, 219
MwoI GCNNNNNNNGC 5 cut(s) 98, 374, 383, 680, 881
NciI CCSGG 1 cut(s) 818
NdeI CATATG 1 cut(s) 763
NdeII GATC 3 cut(s) 304, 625, 936
NlaIII CATG 3 cut(s) 152, 826, 943
NmuCI GTSAC 1 cut(s) 316
PaqCI CACCTGC 1 cut(s) 248
PctI GAATGC 1 cut(s) 756
PfeI GAWTC 2 cut(s) 137, 970
PkrI GCNGC 5 cut(s) 91, 94, 175, 285, 402
PmeI GTTTAAAC 1 cut(s) 43
PshBI ATTAAT 1 cut(s) 291
PsiI TTATAA 1 cut(s) 867
Psp6I CCWGG 2 cut(s) 196, 217
PspGI CCWGG 2 cut(s) 196, 217
PspPI GGNCC 3 cut(s) 110, 156, 556
PsrI GAACNNNNNNTAC 2 cut(s) 513, 545
PstI CTGCAG 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 2 cut(s) 147, 457
SaqAI TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
SatI GCNGC 5 cut(s) 90, 93, 174, 284, 401
Sau3AI GATC 3 cut(s) 304, 625, 936
Sau96I GGNCC 3 cut(s) 110, 156, 556
ScrFI CCNGG 3 cut(s) 198, 219, 818
SduI GDGCHC 3 cut(s) 274, 733, 814
SetI ASST 9 cut(s) 28, 109, 166, 262, 379, 405, 696, 714, 913
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 2 cut(s) 90, 102
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 147, 457
SmlI CTYRAG 2 cut(s) 165, 239
SmoI CTYRAG 2 cut(s) 165, 239
Sse9I AATT 3 cut(s) 299, 347, 579
StyD4I CCNGG 3 cut(s) 196, 217, 816
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 269, 786
TaqI TCGA 2 cut(s) 228, 303
TaqII GACCGA 1 cut(s) 551
TasI AATT 3 cut(s) 299, 347, 579
TfiI GAWTC 2 cut(s) 137, 970
Tru1I TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
Tru9I TTAA 6 cut(s) 42, 240, 291, 396, 419, 689
TscAI CASTG 4 cut(s) 18, 789, 838, 980
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 5 cut(s) 89, 92, 173, 283, 400
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 3 cut(s) 501, 566, 780
TspRI CASTG 4 cut(s) 18, 789, 838, 980
Vha464I CTTAAG 1 cut(s) 239
VneI GTGCAC 2 cut(s) 270, 729
VpaK11BI GGWCC 1 cut(s) 110
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 2 cut(s) 299, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.