FvH4_4g23290

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015263
Physical Location & Seq
Reverse (-)
25820906 .. 25824897
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23290.t1

Sequence Viewer

Length: 1002 bp
ATGGCAATGGCAGTGAGGCTAACACCTGTTCTGGTTCCAGCTTCAGCTCAGTTTAAACAGTTTGCCTTGGTTTCAAGGAAAACCCAGTTCAGGGTCTCTACTGCTGCAACAATGGCTACAGGTGGACCCCAGAGTAGTAGTAAAGTCATTGACTCACATCTCCATGTTTGGGCTTCTCCTCAGGAGGCTGCTGATAAATATCCTTACTTTCCTGGGCAAGAACCCACTTTGCCTGGAGATGTTGATTTATTGCTCAAGTCTATGGAAGAAGCAGGTGTTGACGGTGCACTTATTGTGCAGCCTATCAATCACAAATTCGATCATTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGGAACTGGAGTTAAGGAGCTTGAAAATCTCATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCTTCAGGTCAAAAGATGACCAACGAAGTGGGAAAGGCAATGTTTTCTAAGGCAGGAGAACTTGGAGCACCAGTTGGTTTTATGTGTATGAAGGGACTCGGTCTGCATATATCCGAAATTGAGGAGCTGTGCACAGAGTTTCCATCAACAGTTGTATTGCTCGATCATTTGGCCTTCTGTAAACCACCAATAAATGATGAGGAAAATCAGGCTTTCTCTGCACTCTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAATTAAGTGCCCTTTTCAGGGTGTCAAGAAAGCCATTTCCGTATGACGATATAGCTCATACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCCAACCGTGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCCGAGTGTGGTTATAAAGGAGCCAAAGAAGCCGTTTCTCTGATTGCCAGTCAAATACCTCTATCATCTTCAGAGCTGGACTGGATCATGGGAAGAACAATCTCACAACTCTTTCAGAATCAGTGGCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

334

Amino Acids

36.58

Weight (kDa)

6.66

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 50 - 324 2.5e-30 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AarI CACCTGC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 824
AclWI GGATC 1 cut(s) 959
AcsI RAATTY 2 cut(s) 314, 362
AcuI CTGAAG 4 cut(s) 27, 414, 465, 921
AfiI CCNNNNNNNGG 5 cut(s) 90, 91, 169, 753, 754
AgsI TTSAA 4 cut(s) 75, 346, 422, 463
AjnI CCWGG 2 cut(s) 211, 232
AluBI AGCT 8 cut(s) 41, 47, 392, 418, 604, 709, 791, 943
AluI AGCT 8 cut(s) 41, 47, 392, 418, 604, 709, 791, 943
Alw21I GWGCWC 3 cut(s) 289, 547, 611
Alw26I GTCTC 1 cut(s) 100
Alw44I GTGCAC 2 cut(s) 285, 607
AlwI GGATC 1 cut(s) 959
AoxI GGCC 2 cut(s) 476, 648
ApaLI GTGCAC 2 cut(s) 285, 607
ApeKI GCWGC 3 cut(s) 104, 188, 298
ApoI RAATTY 2 cut(s) 314, 362
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 125
AxyI CCTNAGG 1 cut(s) 180
BaeGI GKGCMC 3 cut(s) 289, 611, 748
BanI GGYRCC 1 cut(s) 824
Bbv12I GWGCWC 3 cut(s) 289, 547, 611
BbvI GCAGC 3 cut(s) 91, 175, 310
BccI CCATC 3 cut(s) 364, 392, 628
BceAI ACGGC 1 cut(s) 884
BciT130I CCWGG 2 cut(s) 213, 234
BciVI GTATCC 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 100
BfmI CTRYAG 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 363
BisI GCNGC 3 cut(s) 105, 189, 299
BlsI GCNGC 3 cut(s) 106, 190, 300
Bme1390I CCNGG 2 cut(s) 213, 234
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 4 cut(s) 36, 127, 826, 889
BmrFI CCNGG 2 cut(s) 213, 234
BmrI ACTGGG 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 79
BpmI CTGGAG 2 cut(s) 255, 426
BpuEI CTTGAG 1 cut(s) 239
BsaBI GATNNNNATC 2 cut(s) 198, 444
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 66, 212
BsaXI ACNNNNNCTCC 2 cut(s) 534, 564
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 91, 169, 753, 754
Bse1I ACTGG 5 cut(s) 85, 409, 548, 915, 953
Bse21I CCTNAGG 1 cut(s) 180
Bse3DI GCAATG 2 cut(s) 12, 522
Bse8I GATNNNNATC 2 cut(s) 198, 444
BseBI CCWGG 2 cut(s) 213, 234
BseDI CCNNGG 2 cut(s) 66, 212
BseJI GATNNNNATC 2 cut(s) 198, 444
BseLI CCNNNNNNNGG 5 cut(s) 90, 91, 169, 753, 754
BseMI GCAATG 2 cut(s) 12, 522
BseMII CTCAG 2 cut(s) 62, 194
BseNI ACTGG 5 cut(s) 85, 409, 548, 915, 953
BseRI GAGGAG 2 cut(s) 168, 614
BseSI GKGCMC 3 cut(s) 289, 611, 748
BseXI GCAGC 3 cut(s) 91, 175, 310
BsgI GTGCAG 2 cut(s) 317, 681
BshFI GGCC 2 cut(s) 478, 650
BshNI GGYRCC 1 cut(s) 824
BsiHKAI GWGCWC 3 cut(s) 289, 547, 611
BslFI GGGAC 2 cut(s) 585, 787
BslI CCNNNNNNNGG 5 cut(s) 90, 91, 169, 753, 754
BsmAI GTCTC 1 cut(s) 100
BsmFI GGGAC 2 cut(s) 585, 787
BsnI GGCC 2 cut(s) 478, 650
Bso31I GGTCTC 1 cut(s) 100
Bsp1286I GDGCHC 4 cut(s) 289, 547, 611, 748
Bsp143I GATC 3 cut(s) 319, 640, 951
BspANI GGCC 2 cut(s) 478, 650
BspCNI CTCAG 2 cut(s) 61, 193
BspLI GGNNCC 4 cut(s) 36, 127, 826, 889
BspMI ACCTGC 1 cut(s) 263
BspPI GGATC 1 cut(s) 959
BspT107I GGYRCC 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 12, 522
BsrI ACTGG 5 cut(s) 85, 409, 548, 915, 953
BssECI CCNNGG 2 cut(s) 66, 212
BssMI GATC 3 cut(s) 319, 640, 951
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 2 cut(s) 213, 234
Bst4CI ACNGT 5 cut(s) 60, 284, 628, 801, 833
BstDEI CTNAG 3 cut(s) 48, 180, 525
BstKTI GATC 3 cut(s) 322, 643, 954
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 3 cut(s) 319, 640, 951
BstMWI GCNNNNNNNGC 4 cut(s) 113, 389, 695, 896
BstNI CCWGG 2 cut(s) 213, 234
BstSCI CCNGG 2 cut(s) 211, 232
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 3 cut(s) 289, 611, 748
BstV1I GCAGC 3 cut(s) 91, 175, 310
BstXI CCANNNNNNTGG 2 cut(s) 474, 505
Bsu36I CCTNAGG 1 cut(s) 180
BsuI GTATCC 1 cut(s) 363
BsuRI GGCC 2 cut(s) 478, 650
BtsI GCAGTG 2 cut(s) 18, 853
BtsIMutI CAGTG 4 cut(s) 18, 797, 853, 995
BveI ACCTGC 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 125
CviAII CATG 3 cut(s) 164, 838, 955
DdeI CTNAG 3 cut(s) 48, 180, 525
DpnI GATC 3 cut(s) 321, 642, 953
DpnII GATC 3 cut(s) 319, 640, 951
DraI TTTAAA 2 cut(s) 55, 435
Eco130I CCWWGG 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 4 cut(s) 27, 414, 465, 921
Eco81I CCTNAGG 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 211, 232
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 3 cut(s) 167, 841, 958
FaqI GGGAC 2 cut(s) 585, 787
FatI CATG 3 cut(s) 163, 837, 954
Fnu4HI GCNGC 3 cut(s) 105, 189, 299
Fsp4HI GCNGC 3 cut(s) 105, 189, 299
GluI GCNGC 3 cut(s) 105, 189, 299
GsuI CTGGAG 2 cut(s) 255, 426
HaeIII GGCC 2 cut(s) 478, 650
Hin1II CATG 3 cut(s) 167, 841, 958
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 3 cut(s) 152, 573, 985
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 5 cut(s) 125, 280, 287, 609, 659
Hpy188I TCNGA 4 cut(s) 592, 909, 940, 984
Hpy188III TCNNGA 3 cut(s) 182, 714, 762
Hpy8I GTNNAC 5 cut(s) 125, 280, 287, 609, 659
HpyAV CCTTC 3 cut(s) 489, 562, 661
HpyCH4III ACNGT 5 cut(s) 60, 284, 628, 801, 833
HpyCH4V TGCA 7 cut(s) 107, 287, 298, 383, 583, 609, 698
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 389, 695, 896
HpyF3I CTNAG 3 cut(s) 48, 180, 525
Hsp92II CATG 3 cut(s) 167, 841, 958
Kzo9I GATC 3 cut(s) 319, 640, 951
LmnI GCTCC 4 cut(s) 415, 542, 601, 887
Lsp1109I GCAGC 3 cut(s) 91, 175, 310
MaeIII GTNAC 1 cut(s) 331
MalI GATC 3 cut(s) 321, 642, 953
MboI GATC 3 cut(s) 319, 640, 951
MboII GAAGA 5 cut(s) 278, 358, 407, 927, 972
MhlI GDGCHC 4 cut(s) 289, 547, 611, 748
MluCI AATT 4 cut(s) 314, 362, 594, 737
MlyI GAGTC 2 cut(s) 146, 567
MnlI CCTC 6 cut(s) 9, 178, 189, 592, 670, 936
MseI TTAA 5 cut(s) 54, 411, 434, 704, 740
MslI CAYNNNNRTG 2 cut(s) 162, 472
MspA1I CMGCKG 1 cut(s) 392
MspR9I CCNGG 2 cut(s) 213, 234
MssI GTTTAAAC 1 cut(s) 55
MvaI CCWGG 2 cut(s) 213, 234
MwoI GCNNNNNNNGC 4 cut(s) 113, 389, 695, 896
NdeII GATC 3 cut(s) 319, 640, 951
NlaIII CATG 3 cut(s) 167, 841, 958
NlaIV GGNNCC 4 cut(s) 36, 127, 826, 889
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 1 cut(s) 331
PaqCI CACCTGC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 985
PflFI GACNNNGTC 1 cut(s) 576
PkrI GCNGC 3 cut(s) 106, 190, 300
PleI GAGTC 2 cut(s) 146, 567
PmeI GTTTAAAC 1 cut(s) 55
PpsI GAGTC 2 cut(s) 146, 567
PsiI TTATAA 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 211, 232
PspGI CCWGG 2 cut(s) 211, 232
PspN4I GGNNCC 4 cut(s) 36, 127, 826, 889
PspPI GGNCC 1 cut(s) 125
PsyI GACNNNGTC 1 cut(s) 576
PvuII CAGCTG 1 cut(s) 392
RseI CAYNNNNRTG 2 cut(s) 162, 472
SaqAI TTAA 5 cut(s) 54, 411, 434, 704, 740
SatI GCNGC 3 cut(s) 105, 189, 299
Sau3AI GATC 3 cut(s) 319, 640, 951
Sau96I GGNCC 1 cut(s) 125
SchI GAGTC 2 cut(s) 146, 567
ScrFI CCNGG 2 cut(s) 213, 234
SduI GDGCHC 4 cut(s) 289, 547, 611, 748
SfcI CTRYAG 1 cut(s) 117
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 162, 472
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 314, 362, 594, 737
StyD4I CCNGG 2 cut(s) 211, 232
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 5 cut(s) 60, 284, 628, 801, 833
TaqI TCGA 2 cut(s) 318, 639
TaqII GACCGA 1 cut(s) 566
TasI AATT 4 cut(s) 314, 362, 594, 737
TfiI GAWTC 1 cut(s) 985
Tru1I TTAA 5 cut(s) 54, 411, 434, 704, 740
Tru9I TTAA 5 cut(s) 54, 411, 434, 704, 740
TscAI CASTG 4 cut(s) 18, 804, 853, 995
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 3 cut(s) 104, 188, 298
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 581, 987
TspGWI ACGGA 1 cut(s) 765
TspRI CASTG 4 cut(s) 18, 804, 853, 995
Tth111I GACNNNGTC 1 cut(s) 576
VneI GTGCAC 2 cut(s) 285, 607
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 2 cut(s) 314, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.