Rh4BG299300

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015263
Physical Location & Seq
Reverse (-)
47076053 .. 47078939
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG299300.1

Sequence Viewer

Length: 831 bp
ATGGCAGTGAGGCTAACACCTGTTCTGGTTCCAGCTTCAGCTAAGTTTAAACACTTTGCCTTCAGTTCAAGAAAAAACCAGTTCAGGTGTATGGAAGAAGCAGGTGTTGACGGTGCACTTATTGTGCAGCCTATTAATCACAAATTCGATCACTCTTTGGTGACAAGTGTGTTGAAGAAGTATCCATCTAAATTTGTTGGTTGTTGCCTTGCAAATCCAGCTGAAGATGGGAGTGGGGTTAAGCAGCTTGAACATCTTATTTTAAAGGATAACTATCGTGCTGTTCGCTTCAATCCATATTTATGGCCATCTGGTCAAAAGATGACCAATGAAGTTGGAAAGGCAATGTTCTCTAAGGCGGGAGAACTTGGAGTACCAGTTGGCTTTATGTGTATGAAGGGCCTCGGTCTGCATATTTCAGAAATTGAGGAACTATGCACAGAGTTTCCTTCAACACTTGTATTACTTGATCATTTGGCTTTCTGCAAACCACCAATAAATGATGAGGAAAATGAGGCTTTCTCTGCACTTTTAAAGCTATCCAGATTTCCACAGGTCTATGTAAAAGTAAGTGCACTTTTCAGGGTGTCAAGAACGCCATTTCCATATGAAGATGTATCTCACACACTGTCCCAAGTTGTGTCAAGTTTTGGTGCTAACCGTGTCATGTGGGGCAGTGATTTTCCATTTGTTGTTGCTGAGTGTGGTTATAAAGGAGCAAAAGAAGCCGTTTCTTTGATTGCCAGTAAAATACCTCTATCATCTTCAGAGTTGGAGTGGATCATGGGTAGAACAATCTTGCAACTCTTCCAGAATCAGTGGCTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

276

Amino Acids

30.73

Weight (kDa)

8.41

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_2 PF04909 31 - 270 5.6e-26 Amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35450
fragaria_vesca FvH4_4g23290 FvH4_4g23290
malus_domestica MD16G1123900.v1.1
prunus_persica Prupe.1G221700_v2.0.a1
pyrus_communis pycom16g10590
rosa_chinensis RchiOBHm_Chr4g0429651
rosa_laevigata RLG00000007055
rosa_multiflora Rmu_sc0006590.1_g000006
rosa_roxburghii Rroxscaffold_5G00371330
rosa_rugosa Rorug04G0237500
rosa_samantha Rh4AG293500 Rh4BG299300 Rh4CG315100 Rh4DG296400
rosa_wichuraiana Rw4G025400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
AarI CACCTGC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 92
AciI CCGC 1 cut(s) 359
AclWI GGATC 1 cut(s) 788
AcoI YGGCCR 1 cut(s) 305
AcsI RAATTY 2 cut(s) 143, 191
AcuI CTGAAG 4 cut(s) 21, 46, 243, 750
AfaI GTAC 1 cut(s) 375
AgsI TTSAA 5 cut(s) 69, 175, 251, 292, 453
AloI GAACNNNNNNTCC 2 cut(s) 586, 618
AluBI AGCT 5 cut(s) 35, 41, 221, 247, 538
AluI AGCT 5 cut(s) 35, 41, 221, 247, 538
Alw21I GWGCWC 2 cut(s) 118, 577
Alw44I GTGCAC 2 cut(s) 114, 573
AlwI GGATC 1 cut(s) 788
AoxI GGCC 2 cut(s) 305, 400
ApaLI GTGCAC 2 cut(s) 114, 573
ApeKI GCWGC 2 cut(s) 127, 244
ApoI RAATTY 2 cut(s) 143, 191
AseI ATTAAT 1 cut(s) 135
AspS9I GGNCC 1 cut(s) 400
AsuHPI GGTGA 1 cut(s) 172
BaeGI GKGCMC 2 cut(s) 118, 577
BalI TGGCCA 1 cut(s) 307
Bbv12I GWGCWC 2 cut(s) 118, 577
BbvI GCAGC 2 cut(s) 139, 256
BccI CCATC 3 cut(s) 193, 221, 316
BceAI ACGGC 1 cut(s) 713
BciVI GTATCC 1 cut(s) 192
BclI TGATCA 1 cut(s) 469
BfuAI ACCTGC 1 cut(s) 92
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 2 cut(s) 128, 245
BlsI GCNGC 2 cut(s) 129, 246
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 506, 538
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bse1I ACTGG 3 cut(s) 79, 377, 744
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 403
BseJI GATNNNNATC 1 cut(s) 273
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 690
BseNI ACTGG 3 cut(s) 79, 377, 744
BseSI GKGCMC 2 cut(s) 118, 577
BseXI GCAGC 2 cut(s) 139, 256
BsgI GTGCAG 2 cut(s) 146, 510
BshFI GGCC 2 cut(s) 307, 402
BsiHKAI GWGCWC 2 cut(s) 118, 577
BslFI GGGAC 1 cut(s) 616
BsmFI GGGAC 1 cut(s) 616
BsnI GGCC 2 cut(s) 307, 402
Bsp1286I GDGCHC 2 cut(s) 118, 577
Bsp143I GATC 3 cut(s) 148, 469, 780
BspACI CCGC 1 cut(s) 359
BspANI GGCC 2 cut(s) 307, 402
BspCNI CTCAG 1 cut(s) 691
BspLI GGNNCC 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 92
BspPI GGATC 1 cut(s) 788
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 3 cut(s) 79, 377, 744
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 3 cut(s) 148, 469, 780
Bst4CI ACNGT 3 cut(s) 113, 630, 662
Bst6I CTCTTC 1 cut(s) 812
BstDEI CTNAG 3 cut(s) 42, 354, 699
BstKTI GATC 3 cut(s) 151, 472, 783
BstMBI GATC 3 cut(s) 148, 469, 780
BstMWI GCNNNNNNNGC 3 cut(s) 218, 524, 725
BstSLI GKGCMC 2 cut(s) 118, 577
BstV1I GCAGC 2 cut(s) 139, 256
BstXI CCANNNNNNTGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 2 cut(s) 307, 402
BtsI GCAGTG 2 cut(s) 12, 682
BtsIMutI CAGTG 4 cut(s) 12, 626, 682, 824
BveI ACCTGC 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 374
CviAII CATG 2 cut(s) 667, 784
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 3 cut(s) 42, 354, 699
DpnI GATC 3 cut(s) 150, 471, 782
DpnII GATC 3 cut(s) 148, 469, 780
DraI TTTAAA 3 cut(s) 49, 264, 534
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 812
EarI CTCTTC 1 cut(s) 812
Eco57I CTGAAG 4 cut(s) 21, 46, 243, 750
EcoO109I RGGNCCY 1 cut(s) 400
FaeI CATG 2 cut(s) 670, 787
FaqI GGGAC 1 cut(s) 616
FatI CATG 2 cut(s) 666, 783
FauI CCCGC 1 cut(s) 352
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 128, 245
Fsp4HI GCNGC 2 cut(s) 128, 245
GluI GCNGC 2 cut(s) 128, 245
HaeIII GGCC 2 cut(s) 307, 402
Hin1II CATG 2 cut(s) 670, 787
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 1 cut(s) 814
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 3 cut(s) 109, 116, 575
Hpy188I TCNGA 2 cut(s) 421, 769
Hpy188III TCNNGA 4 cut(s) 69, 543, 591, 811
Hpy8I GTNNAC 3 cut(s) 109, 116, 575
HpyAV CCTTC 3 cut(s) 70, 391, 459
HpyCH4III ACNGT 3 cut(s) 113, 630, 662
HpyCH4V TGCA 9 cut(s) 116, 127, 212, 412, 438, 486, 527, 575, 802
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 524, 725
HpyF3I CTNAG 3 cut(s) 42, 354, 699
Hsp92II CATG 2 cut(s) 670, 787
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 148, 469, 780
LmnI GCTCC 1 cut(s) 716
Lsp1109I GCAGC 2 cut(s) 139, 256
MaeIII GTNAC 1 cut(s) 160
MalI GATC 3 cut(s) 150, 471, 782
MboI GATC 3 cut(s) 148, 469, 780
MboII GAAGA 6 cut(s) 107, 187, 236, 623, 756, 799
MhlI GDGCHC 2 cut(s) 118, 577
MlsI TGGCCA 1 cut(s) 307
MluCI AATT 3 cut(s) 143, 191, 423
MluNI TGGCCA 1 cut(s) 307
MmeI TCCRAC 2 cut(s) 316, 753
MnlI CCTC 6 cut(s) 3, 413, 421, 499, 508, 765
Mox20I TGGCCA 1 cut(s) 307
MscI TGGCCA 1 cut(s) 307
MseI TTAA 5 cut(s) 48, 135, 240, 263, 533
MslI CAYNNNNRTG 1 cut(s) 301
Msp20I TGGCCA 1 cut(s) 307
MspA1I CMGCKG 1 cut(s) 221
MssI GTTTAAAC 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 218, 524, 725
NdeI CATATG 1 cut(s) 607
NdeII GATC 3 cut(s) 148, 469, 780
NlaIII CATG 2 cut(s) 670, 787
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 160
PaqCI CACCTGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 814
PkrI GCNGC 2 cut(s) 129, 246
PmeI GTTTAAAC 1 cut(s) 49
PshBI ATTAAT 1 cut(s) 135
PsiI TTATAA 1 cut(s) 711
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 400
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 301
SaqAI TTAA 5 cut(s) 48, 135, 240, 263, 533
SatI GCNGC 2 cut(s) 128, 245
Sau3AI GATC 3 cut(s) 148, 469, 780
Sau96I GGNCC 1 cut(s) 400
SduI GDGCHC 2 cut(s) 118, 577
SmiMI CAYNNNNRTG 1 cut(s) 301
Sse9I AATT 3 cut(s) 143, 191, 423
SsiI CCGC 1 cut(s) 359
TaaI ACNGT 3 cut(s) 113, 630, 662
TaqI TCGA 1 cut(s) 147
TaqII GACCGA 1 cut(s) 395
TasI AATT 3 cut(s) 143, 191, 423
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 5 cut(s) 48, 135, 240, 263, 533
Tru9I TTAA 5 cut(s) 48, 135, 240, 263, 533
TscAI CASTG 4 cut(s) 12, 633, 682, 824
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 2 cut(s) 127, 244
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 3 cut(s) 345, 410, 624
TspRI CASTG 4 cut(s) 12, 633, 682, 824
VneI GTGCAC 2 cut(s) 114, 573
VspI ATTAAT 1 cut(s) 135
XapI RAATTY 2 cut(s) 143, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.